公开(公告)号：US06338802B1

公开(公告)日：2002-01-15

申请号：US09565566

申请日：2000-05-04

申请人： Kevin S. Bodner ,  Alfred P. Madden ,  Joseph Jackson ,  Jason H. Halsey ,  Mark T. Reed ,  Ward Frye ,  Mark F. Oldham ,  Stephen E. Moring ,  Jon Hoshizaki

发明人： Kevin S. Bodner ,  Alfred P. Madden ,  Joseph Jackson ,  Jason H. Halsey ,  Mark T. Reed ,  Ward Frye ,  Mark F. Oldham ,  Stephen E. Moring ,  Jon Hoshizaki

IPC分类号： B01D6100

CPC分类号： B01L3/5025 ,  B01D61/18 ,  B01L3/50255 ,  B01L2400/049 ,  G01N35/0099 ,  G01N35/028 ,  G01N35/1074 ,  G01N2035/00485 ,  G01N2035/102 ,  Y10T436/25

摘要： The present invention provides multi-well plates and column arrays in which samples (e.g., cell lysates containing nucleic acids of interest, such as RNA) can be analyzed and/or processed. In one embodiment, the microfiltration arrangement is a multilayer structure, including (i) a column plate having an array of minicolumns into which samples can be placed, (ii) a discrete filter element disposed in each minicolumn, (iii) a drip-director plate having a corresponding array of drip directors through which filtrate may egress, and (iv) a receiving-well plate having a corresponding array of receiving wells into which filtrate can flow. The invention provides multi-well microfiltration arrangements that are relatively simple to manufacture and that overcome many of the problems associated with the prior arrangements relating to (i) cross-contamination due to wicking across a common filter sheet or (ii) individual filter elements entrapping sample constituents within substantial dead volumes. Further, the invention provides multi-well microfiltration arrangements that adequately support discrete filter elements disposed in the wells without creating substantial preferential flow. Additionally, the invention provides multi-well microfiltration arrangements that avoid cross-contamination due to aerosol formation, pendent drops and/or splattering. Other disclosed features of the invention provide for the automated covering or heat-sealing of filtrate samples separately collected in an array of wells.

摘要翻译： 本发明提供了多孔板和柱阵列，其中可以分析和/或处理样品（例如，含有目标核酸的细胞裂解物，例如RNA）。 在一个实施方案中，微滤装置是多层结构，包括（i）具有可以放置样品的小柱阵列的柱板，（ii）设置在每个小柱中的离散过滤元件，（iii）滴液导向器 板具有相应的滴液导向器阵列，滤液可以通过其排出;以及（iv）接收孔板，其具有滤液能够流过的相应阵列的接收孔。 本发明提供了相对简单的多孔微量过滤装置，并且克服了许多与先前布置有关的问题，这些问题涉及（i）由于芯毛穿过公共过滤片引起的交叉污染或（ii）截留的单个过滤器元件 大量死体积的样品成分。 此外，本发明提供了多孔微过滤装置，其充分地支撑设置在井中的分立的过滤元件，而不产生实质的优先流动。 此外，本发明提供了多孔微滤装置，其避免了由于气溶胶形成，悬垂滴和/或飞溅而导致的交叉污染。 本发明的其它公开的特征提供了分别收集在孔阵列中的滤液样品的自动覆盖或热封。

公开(公告)号：US06451261B1

公开(公告)日：2002-09-17

申请号：US09565673

申请日：2000-05-04

申请人： Kevin S. Bodner ,  Alfred P. Madden ,  Joseph Jackson ,  Jason H. Halsey ,  Mark T. Reed ,  Ward Frye ,  Mark F. Oldham ,  Stephen E. Moring ,  Jon Hoshizaki

发明人： Kevin S. Bodner ,  Alfred P. Madden ,  Joseph Jackson ,  Jason H. Halsey ,  Mark T. Reed ,  Ward Frye ,  Mark F. Oldham ,  Stephen E. Moring ,  Jon Hoshizaki

IPC分类号： B01L300

CPC分类号： B01L3/5025 ,  B01D61/18 ,  B01L3/50255 ,  B01L2400/049 ,  G01N35/0099 ,  G01N35/028 ,  G01N35/1074 ,  G01N2035/00485 ,  G01N2035/102 ,  Y10T436/25

摘要： The present invention provides multi-well plates and column arrays in which samples (e.g., cell lysates containing nucleic acids of interest, such as RNA) can be analyzed and/or processed. In one embodiment, the microfiltration arrangement is a multilayer structure, including (i) a column plate having an array of minicolumns into which samples can be placed, (ii) a discrete filter element disposed in each minicolumn, (iii) a drip-director plate having a corresponding array of drip directors through which filtrate may egress, and (iv) a receiving-well plate having a corresponding array of receiving wells into which filtrate can flow. The invention provides multi-well microfiltration arrangements that are relatively simple to manufacture and that overcome many of the problems associated with the prior arrangements relating to (i) cross-contamination due to wicking across a common filter sheet or (ii) individual filter elements entrapping sample constituents within substantial dead volumes. Further, the invention provides multi-well microfiltration arrangements that adequately support discrete filter elements disposed in the wells without creating substantial preferential flow. Additionally, the invention provides multi-well microfiltration arrangements that avoid cross-contamination due to aerosol formation, pendent drops and/or splattering. Other disclosed features of the invention provide for the automated covering or heat-sealing of filtrate samples separately collected in an array of wells.

公开(公告)号：US6159368A

公开(公告)日：2000-12-12

申请号：US182946

申请日：1998-10-29

申请人： Stephen E. Moring ,  Kevin S. Bodner ,  Jason H. Halsey ,  Jon Hoshizaki ,  Joseph Jackson ,  Alfred P. Madden ,  Mark F. Oldham ,  Mark T. Reed

发明人： Stephen E. Moring ,  Kevin S. Bodner ,  Jason H. Halsey ,  Jon Hoshizaki ,  Joseph Jackson ,  Alfred P. Madden ,  Mark F. Oldham ,  Mark T. Reed

IPC分类号： G01N1/00 ,  B01D29/00 ,  B01D35/02 ,  B01D53/22 ,  B01D61/18 ,  B01L3/00 ,  C12N15/09 ,  G01N35/00 ,  G01N35/02 ,  G01N35/10 ,  B01D63/00

CPC分类号： B01L3/5025 ,  B01D61/18 ,  B01L3/50255 ,  G01N35/0099 ,  B01L2400/049 ,  G01N2035/00485 ,  G01N2035/102 ,  G01N35/028 ,  G01N35/1074 ,  Y10T436/25

摘要： The present invention provides multi-well plates and column arrays in which samples (e.g., cell lysates containing nucleic acids of interest, such as RNA) can be analyzed and/or processed. In one embodiment, the microfiltration arrangement is a multilayer structure, including (i) a column plate having an array of minicolumns into which samples can be placed, (ii) a discrete filter element disposed in each minicolumn, (iii) a drip-director plate having a corresponding array of drip directors through which filtrate may egress, and (iv) a receiving-well plate having a corresponding array of receiving wells into which filtrate can flow. The invention provides multi-well microfiltration arrangements that are relatively simple to manufacture and that overcome many of the problems associated with is the prior arrangements relating to (i) cross-contamination due to wicking across a common filter sheet or (ii) individual filter elements entrapping sample constituents within substantial dead volumes. Further, the invention provides multi-well microfiltration arrangements that adequately support discrete filter elements disposed in the wells without creating substantial preferential flow. Additionally, the invention provides multi-well microfiltration arrangements that avoid cross-contamination due to aerosol formation, pendent drops and/or splattering. Other disclosed features of the invention provide for the automated covering or heat-sealing of filtrate samples separately collected in an array of wells.

摘要翻译： 本发明提供了多孔板和柱阵列，其中可以分析和/或处理样品（例如，含有目标核酸的细胞裂解物，例如RNA）。 在一个实施方案中，微滤装置是多层结构，包括（i）具有可以放置样品的小柱阵列的柱板，（ii）设置在每个小柱中的离散过滤元件，（iii）滴液导向器 板具有相应的滴液导向器阵列，滤液可以通过其排出;以及（iv）接收孔板，其具有滤液能够流过的相应阵列的接收孔。 本发明提供了相对简单的多孔微过滤装置，并且克服了许多与以下问题有关的问题：（i）由于穿过公共过滤片芯线而引起的交叉污染或（ii）单个过滤元件 在大量死体积内捕获样品成分。 此外，本发明提供了多孔微过滤装置，其充分地支撑设置在井中的分立的过滤元件，而不产生实质的优先流动。 另外，本发明提供了多孔微量过滤装置，其避免了由于气溶胶形成，悬挂液滴和/或飞溅而导致的交叉污染。 本发明的其它公开的特征提供了分别收集在孔阵列中的滤液样品的自动覆盖或热封。

公开(公告)号：US06783732B2

公开(公告)日：2004-08-31

申请号：US10199743

申请日：2002-07-19

申请人： Alfred P Madden ,  Jon Hoshizaki ,  Jason H. Halsey ,  Stephen E. Moring ,  Mark F. Oldham ,  Kevin S. Bodner

发明人： Alfred P Madden ,  Jon Hoshizaki ,  Jason H. Halsey ,  Stephen E. Moring ,  Mark F. Oldham ,  Kevin S. Bodner

IPC分类号： G01N3500

CPC分类号： B01L3/5025 ,  B01D61/18 ,  B01L3/50255 ,  B01L2400/049 ,  G01N35/0099 ,  G01N35/028 ,  G01N35/1074 ,  G01N2035/00485 ,  G01N2035/102 ,  Y10T436/25

摘要： The present invention provides multi-well plates and column arrays in which samples (e.g., cell lysates containing nucleic acids of interest, such as RNA) can be analyzed and/or processed. In one embodiment, the microfiltration arrangement is a multilayer structure, including (i) a column plate having an array of minicolumns into which samples can be placed, (ii) a discrete filter element disposed in each minicolumn, (iii) a drip-director plate having a corresponding array of drip directors through which filtrate may egress, and (iv) a receiving-well plate having a corresponding array of receiving wells into which filtrate can flow. The invention provides multi-well microfiltration arrangements that are relatively simple to manufacture and that overcome many of the problems associated with the prior arrangements relating to (i) cross-contamination due to wicking across a common filter sheet or (ii) individual filter elements entrapping sample constituents within substantial dead volumes. Further, the invention provides multi-well microfiltration arrangements that adequately support discrete filter elements disposed in the wells without creating substantial preferential flow. Additionally, the invention provides multi-well microfiltration arrangements that avoid cross-contamination due to aerosol formation, pendent drops and/or splattering. Other disclosed features of the invention provide for the automated covering or heat-sealing of filtrate samples separately collected in an array of wells.

摘要翻译： 本发明提供了多孔板和柱阵列，其中可以分析和/或处理样品（例如，含有目标核酸的细胞裂解物，例如RNA）。 在一个实施方案中，微滤装置是多层结构，包括（i）具有可以放置样品的小柱阵列的柱板，（ii）设置在每个小柱中的离散过滤元件，（iii）滴液导向器 板具有相应的滴液导向器阵列，滤液可以通过其排出;以及（iv）接收孔板，其具有滤液能够流过的相应阵列的接收孔。 本发明提供了相对简单的多孔微量过滤装置，并且克服了许多与先前布置有关的问题，这些问题涉及（i）由于芯毛穿过公共过滤片引起的交叉污染或（ii）截留的单个过滤器元件 大量死体积的样品成分。 此外，本发明提供了多孔微过滤装置，其充分地支撑设置在井中的分立的过滤元件，而不产生实质的优先流动。 此外，本发明提供了多孔微滤装置，其避免了由于气溶胶形成，悬垂滴和/或飞溅而导致的交叉污染。 本发明的其它公开的特征提供了分别收集在孔阵列中的滤液样品的自动覆盖或热封。

公开(公告)号：US06506343B1

公开(公告)日：2003-01-14

申请号：US09565202

申请日：2000-05-04

申请人： Kevin S. Bodner ,  Alfred P. Madden ,  Jason H. Halsey ,  Mark F. Oldham ,  Stephen E. Moring ,  Jon Hoshizaki

发明人： Kevin S. Bodner ,  Alfred P. Madden ,  Jason H. Halsey ,  Mark F. Oldham ,  Stephen E. Moring ,  Jon Hoshizaki

IPC分类号： B01D3500

CPC分类号： B01L3/5025 ,  B01D61/18 ,  B01L3/50255 ,  B01L2400/049 ,  G01N35/0099 ,  G01N35/028 ,  G01N35/1074 ,  G01N2035/00485 ,  G01N2035/102 ,  Y10T436/25

摘要： The present invention provides multi-well plates and column arrays in which samples (e.g., cell lysates containing nucleic acids of interest, such as RNA) can be analyzed and/or processed. In one embodiment, the microfiltration arrangement is a multilayer structure, including (i) a column plate having an array of minicolumns into which samples can be placed, (ii) a discrete filter element disposed in each minicolumn, (iii) a drip-director plate having a corresponding array of drip directors through which filtrate may egress, and (iv) a receiving-well plate having a corresponding array of receiving wells into which filtrate can flow. The invention provides multi-well microfiltration arrangements that are relatively simple to manufacture and that overcome many of the problems associated with the prior arrangements relating to (i) cross-contamination due to wicking across a common filter sheet or (ii) individual filter elements entrapping sample constituents within substantial dead volumes. Further, the invention provides multi-well microfiltration arrangements that adequately support discrete filter elements disposed in the wells without creating substantial preferential flow. Additionally, the invention provides multi-well microfiltration arrangements that avoid cross-contamination due to aerosol formation, pendent drops and/or splattering. Other disclosed features of the invention provide for the automated covering or heat-sealing of filtrate samples separately collected in an array of wells.

摘要翻译： 本发明提供了多孔板和柱阵列，其中可以分析和/或处理样品（例如，含有目标核酸的细胞裂解物，例如RNA）。 在一个实施方案中，微滤装置是多层结构，包括（i）具有可以放置样品的小柱阵列的柱板，（ii）设置在每个小柱中的离散过滤元件，（iii）滴液导向器 板具有相应的滴液导向器阵列，滤液可以通过其排出;以及（iv）接收孔板，其具有滤液能够流过的相应阵列的接收孔。 本发明提供了相对简单的多孔微量过滤装置，并且克服了许多与先前布置有关的问题，这些问题涉及（i）由于芯毛穿过公共过滤片引起的交叉污染或（ii）截留的单个过滤器元件 大量死体积的样品成分。 此外，本发明提供了多孔微过滤装置，其充分地支撑设置在井中的分立的过滤元件，而不产生实质的优先流动。 此外，本发明提供了多孔微滤装置，其避免了由于气溶胶形成，悬垂滴和/或飞溅而导致的交叉污染。 本发明的其它公开的特征提供了分别收集在孔阵列中的滤液样品的自动覆盖或热封。

公开(公告)号：US5384024A

公开(公告)日：1995-01-24

申请号：US850764

申请日：1992-03-13

申请人： Stephen E. Moring ,  Michael S. Albin ,  Reid B. Kowallis ,  Thomas E. Lee ,  Dennis E. Mead ,  John H. Nickel ,  Mark F. Oldham ,  Richard T. Reel ,  Timothy S. Orpin ,  Janice C. Woods

发明人： Stephen E. Moring ,  Michael S. Albin ,  Reid B. Kowallis ,  Thomas E. Lee ,  Dennis E. Mead ,  John H. Nickel ,  Mark F. Oldham ,  Richard T. Reel ,  Timothy S. Orpin ,  Janice C. Woods

IPC分类号： G01N27/447 ,  B01D61/46 ,  B01D57/02 ,  G01N27/26

CPC分类号： G01N27/44782

摘要： An instrument for capillary electrophoresis has a vertically translatable carrier holding one end of the capillary and an electrode connected to a power supply, and a rotary carousel for presenting containers of sample and buffer solutions to a load position where the end of the capillary and the electrode may be inserted by operating the carrier. The other end of the capillary and a second electrode connected to the power supply are immersed in an outlet buffer reservoir. The instrument has a vacuum supply for providing a relative vacuum over the solution in the outlet buffer reservoir, so simple material may be injected into the capillary either by electromigration or by differential pressure. After injection, the first end of the capillary and the electrode may be immersed in buffer to accomplish electrophoresis. By manipulating the carousel and carrier, multiple samples may be electrophoresed in series. In a preferred embodiment the instrument includes a flexible duck-billed septum for closing containers while permitting easy access for the electrode and the capillary tube. Also in a preferred embodiment the instrument is controlled by a microprocessor-based controller.

摘要翻译： 用于毛细管电泳的仪器具有保持毛细管的一端和连接到电源的电极的可垂直平移的载体，以及用于将样品和缓冲溶液的容器呈现到毛细管和电极的端部的负载位置的旋转转盘 可以通过操作载体来插入。 毛细管的另一端和连接到电源的第二电极浸入出口缓冲储存器中。 该仪器具有用于在出口缓冲储存器中的溶液上提供相对真空的真空源，因此可以通过电迁移或通过差压将简单的材料注入毛细管。 注射后，将毛细管和电极的第一端浸入缓冲液中以完成电泳。 通过操纵转盘和载体，可以将多个样品串联进行电泳。 在优选实施例中，该仪器包括用于封闭容器的柔性鸭嘴孔隔膜，同时允许容易地接近电极和毛细管。 同样在优选实施例中，仪器由基于微处理器的控制器控制。

公开(公告)号：US5972613A

公开(公告)日：1999-10-26

申请号：US987329

申请日：1997-12-09

申请人： Ralph Somack ,  Stephen E. Moring

发明人： Ralph Somack ,  Stephen E. Moring

IPC分类号： C12N15/09 ,  C07H1/06 ,  C07H21/00 ,  C12N15/10 ,  C12Q1/68

CPC分类号： C12N15/1003 ,  C12Q1/6806 ,  Y10S435/803

摘要： The invention relates to new methods of RNA isolation that exploit the surprising discovery that RNA may be differentially precipitated from DNA. The subject methods result in the formation of an RNA-containing precipitate that has an RNA content at least two fold enriched with respect to DNA, as compared with the RNA to DNA ratio of the solution from which the RNA-containing precipitate is derived. The invention also includes various methods of DNA isolation that employ the selective precipitation of RNA. The degree of RNA enrichment achieved is often much greater than two fold; enrichment by a factor of ten or greater is frequently obtained. By precipitating RNA from a solution, the RNA may be collected by simple procedures such as centrifugation or filtration, thereby avoiding the need to bind the RNA to solid phase. The collected RNA precipitate may then be solubilized. One embodiment of the subject methods involves forming an RNA-containing nucleic acid composition, adding an RNA precipitate forming chaotropic salt to the nucleic acid composition in a sufficient concentration to form an RNA-containing precipitate, collecting the RNA-containing precipitate, and (optionally) solubilizing the precipitate. Preferred precipitate forming chaotropic salts are guanidine hydrochloride and guanidine thiocyanate. Other embodiments of the invention include kits for performing the methods of the invention. The invention also includes systems for performing the methods of the invention.

摘要翻译： 本发明涉及RNA分离的新方法，其利用RNA可能从DNA差异沉淀的惊人发现。 与衍生含RNA的沉淀物的溶液的RNA与DNA的比例相比，主题方法导致形成含RNA沉淀物，其RNA含量相对于DNA富集至少两倍。 本发明还包括采用RNA选择性沉淀的各种DNA分离方法。 实现的RNA浓缩程度通常远大于2倍; 浓缩十倍以上时经常得到。 通过从溶液中沉淀RNA，可以通过简单的方法如离心或过滤收集RNA，从而避免将RNA结合到固相。 然后可以将收集的RNA沉淀物溶解。 本发明方法的一个实施方案包括形成含RNA的核酸组合物，将足够浓度的形成离液盐的RNA沉淀物加入到核酸组合物中以形成含RNA沉淀物，收集含RNA沉淀物，和（任选地 ）溶解沉淀物。 形成离液盐的优选沉淀物是盐酸胍和硫氰酸胍。 本发明的其它实施方案包括用于实施本发明方法的试剂盒。 本发明还包括用于执行本发明的方法的系统。

公开(公告)号：US5274227A

公开(公告)日：1993-12-28

申请号：US965827

申请日：1992-10-23

申请人： Stephen E. Moring

发明人： Stephen E. Moring

IPC分类号： G01N30/60 ,  G01N21/03 ,  G01N21/05 ,  G01N30/74 ,  H01J5/16

CPC分类号： G01N21/05 ,  G01N2021/0346 ,  G01N2021/052 ,  G01N21/0303

摘要： A detector cell, having a capillary entrance elbow through which a beam of exposing light can be directed into the bore of a central leg of the detector cell capillary. Ray traces, to determine the effect of the entrance elbow on the beam of exposing light are used to optimize performance of this detector cell. Back ray tracing is implemented to illustrate an optimized embodiment. Forward ray tracing is also illustrated and can be used to determine system performance for a range or choices of a single parameter or for concurrent ranges of choices of a subset of the parameters of the beam of exposing light.

公开(公告)号：US5269599A

公开(公告)日：1993-12-14

申请号：US907130

申请日：1992-07-01

申请人： Stephen E. Moring

发明人： Stephen E. Moring

IPC分类号： A47B46/00 ,  A47B81/00

CPC分类号： A47B46/005 ,  Y10S312/902

摘要： A cabinet designed for the storage of basic kitchen utensils, cookware and food stuffs which is designed for use by campers and work crews in remote locations. It consists of a trunk-like cabinet with multiple compartments, a convertible top and front panels which open into a portable kitchen. Unique features include a drawer and slideable trays for maximum accessibility of contents, a paper towel rack, fluorescent lamp and convertible utility compartment.

摘要翻译： 设计用于储存基本厨房用具，炊具和食品的柜子，专为远程位置的露营者和工作人员使用而设计。 它包括一个带有多个隔间的行李箱，一个可转换的顶部和前面板，通向便携式厨房。 独特的功能包括一个抽屉和可滑动的托盘，最大限度地提供内容，纸巾架，荧光灯和敞篷车厢。

公开(公告)号：US5110431A

公开(公告)日：1992-05-05

申请号：US486893

申请日：1990-02-28

申请人： Stephen E. Moring

发明人： Stephen E. Moring

IPC分类号： G01N27/447 ,  G01N30/02 ,  G01N30/28 ,  G01N30/60 ,  G01N30/84

CPC分类号： G01N27/44717 ,  G01N27/44721 ,  G01N27/44773 ,  G01N2030/285 ,  G01N2030/8435 ,  G01N30/02 ,  G01N30/60

摘要： A junction reactor that aligns a pair of capillaries substantially collinearly, end-to-end that allows a small gap to be produced between these two ends. An applied voltage difference between the other ends of these two capillaries produces in the gap electric field lines that extend across the gap. Empirical evidence shows that the gap introduces only a small reduction in resolution of an electrophoretic or electrochromatographic separation. The gap enables sample liquid to be coupled between capillaries of different internal diameters and enables on-capillary reactions such as attaching a fluorescent tag to a sample components.

摘要翻译： 连接反应器使一对毛细管基本上共线地对齐，端对端，允许在这两个端部之间产生小的间隙。 这两个毛细管的另一端之间施加的电压差在间隙中产生延伸的间隙的电场线。 经验证据表明，差距仅仅导致电泳或电色谱分离的分辨率降低。 该间隙使得样品液体能够连接在不同内径的毛细管之间，并使得能够进行毛细管反应，例如将荧光标签附接到样品组分。