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公开(公告)号：US20110014617A1
公开(公告)日：2011-01-20
申请号：US12816006
申请日：2010-06-15
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6834 , C12Q1/6823 , C12Q1/6825 , C12Q2531/113 , C12Q2521/307 , C12Q2525/204 , C12Q2565/607 , C12Q2561/109 , C12Q2565/519
摘要： Methods and kits for detecting a target nucleic acid in a sample are described. In some embodiments, the sample to be analyzed includes a primer which hybridizes to at least a portion of the target nucleic acid, a probe having a first region which hybridizes to at least a portion of the target nucleic acid and a second region having a detectable label, a polymerase which extends the hybridized primer and an enzyme comprising nuclease activity that can cleave the hybridized hybridization probe to thereby release a labeled probe fragment. In some embodiments, the sample can then be contacted with a solid support comprising surface bound capture probes which can hybridize to the labeled probe fragment(s). These capture probes more readily bind to the probe fragment(s) than to the intact hybridization probe. The label can then be detected on the support surface. In this manner, improved discrimination between the probe fragments and the intact hybridization probes can be achieved.
摘要翻译： 描述了用于检测样品中靶核酸的方法和试剂盒。 在一些实施方案中，待分析的样品包括与靶核酸的至少一部分杂交的引物，具有与靶核酸的至少一部分杂交的第一区的探针和具有可检测的第二区 标记，扩增杂交引物的聚合酶和包含核酸酶活性的酶，其可以切割杂交的杂交探针，从而释放标记的探针片段。 在一些实施方案中，然后可以将样品与包含可以与标记的探针片段杂交的表面结合捕获探针的固体支持物接触。 这些捕获探针比完整的杂交探针更容易与探针片段结合。 然后可以在支撑表面上检测标签。 以这种方式，可以实现探针片段与完整杂交探针之间的改善的区别。
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公开(公告)号：US20080241838A1
公开(公告)日：2008-10-02
申请号：US11965837
申请日：2007-12-28
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6834 , C12Q1/6823 , C12Q1/6825 , C12Q2531/113 , C12Q2521/307 , C12Q2525/204 , C12Q2565/607 , C12Q2561/109 , C12Q2565/519
摘要： Methods and kits for detecting a target nucleic acid in a sample are described. In some embodiments, the sample to be analyzed includes a primer which hybridizes to at least a portion of the target nucleic acid, a probe having a first region which hybridizes to at least a portion of the target nucleic acid and a second region having a detectable label, a polymerase which extends the hybridized primer and an enzyme comprising nuclease activity that can cleave the hybridized hybridization probe to thereby release a labeled probe fragment. In some embodiments, the sample can then be contacted with a solid support comprising surface bound capture probes which can hybridize to the labeled probe fragment(s). These capture probes more readily bind to the probe fragment(s) than to the intact hybridization probe. The label can then be detected on the support surface. In this manner, improved discrimination between the probe fragments and the intact hybridization probes can be achieved.
摘要翻译： 描述了用于检测样品中靶核酸的方法和试剂盒。 在一些实施方案中，待分析的样品包括与靶核酸的至少一部分杂交的引物，具有与靶核酸的至少一部分杂交的第一区的探针和具有可检测的第二区 标记，扩增杂交引物的聚合酶和包含核酸酶活性的酶，其可以切割杂交的杂交探针，从而释放标记的探针片段。 在一些实施方案中，然后可以将样品与包含可以与标记的探针片段杂交的表面结合捕获探针的固体支持物接触。 这些捕获探针比完整的杂交探针更容易与探针片段结合。 然后可以在支撑表面上检测标签。 以这种方式，可以实现探针片段与完整杂交探针之间的改善的区别。
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公开(公告)号：US20070099211A1
公开(公告)日：2007-05-03
申请号：US11488439
申请日：2006-07-17
申请人： Vissarion Aivazachvili , Kristian Scaboo , Aldrich Lau , Konrad Faulstich , Robert Eason , John Van Camp , Timothy Liu
发明人： Vissarion Aivazachvili , Kristian Scaboo , Aldrich Lau , Konrad Faulstich , Robert Eason , John Van Camp , Timothy Liu
CPC分类号： C12Q1/6823 , B01L3/5027 , B01L7/525 , B01L2200/10 , B01L2300/0645 , B01L2300/0681 , B01L2300/1844 , B01L2300/185 , B01L2300/1872 , B01L2300/1894 , B01L2400/0418 , C12Q1/6825 , C12Q2565/607 , C12Q2563/131 , C12Q2565/629 , C12Q2531/113 , C12Q2521/301 , C12Q2523/107
摘要： Methods for detecting a target polynucleotide sequences are provided that utilize a probe having a target-complementary segment and a detectable tag. By cleaving the detectable tab and associating the tag with a tag complement coupled to an electrode, an electrochemical signal can be detected that is related to the presence of the tag:tag complement complex.
摘要翻译： 提供了检测靶多核苷酸序列的方法，其利用具有靶互补区段和可检测标签的探针。 通过切割可检测的标签并将标签与耦合到电极的标签补体相关联，可以检测到与标签：标签补体复合物的存在相关的电化学信号。
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公开(公告)号：US20120045767A1
公开(公告)日：2012-02-23
申请号：US13215157
申请日：2011-08-22
申请人： Aldrich LAU , Robert Eason , Maxim Brevnov , Handong Li , Kevin Hacker
发明人： Aldrich LAU , Robert Eason , Maxim Brevnov , Handong Li , Kevin Hacker
CPC分类号： G01N33/54326 , C12N1/02 , C12N13/00 , C12Q1/689 , G01N33/54353 , G01N33/56911
摘要： Magnetic beads that include polyvalent ligands comprising various carbohydrates are described. Methods for fabricating such magnetic beads are also provided as well as methods of their use to capture and enrich pathogen cell population for subsequent culture, lysis and identification.
摘要翻译： 描述包括包含各种碳水化合物的多价配体的磁珠。 还提供了制备这种磁珠的方法以及它们用于捕获和富集病原体细胞群以用于随后的培养，裂解和鉴定的方法。
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公开(公告)号：US06952307B2
公开(公告)日：2005-10-04
申请号：US10481902
申请日：2002-06-26
CPC分类号： G02F1/3558 , G02F1/3775
摘要： A method of inducing a periodic variation of nonlinearity in a sample of ferroelectric material, comprises applying an electrically insulting mask to surface of the sample, applying an electric field across the sample to produce domain inversion in the sample, and removing the electric field when non-inverted regions of the sample remain only in the vicinity of the surface of the sample beneath parts of the surface covered by the mask. This method can be used to engineer accurate domain periods of submicron dimensions or larger which are confined to a surface region of the ferroelectric material, so that the poled material can be used to fabricate planar waveguide devices for nonlinear optical applications. In particular, the submicron periods can be exploited in the fabrication of one and two-dimensional photonic band gap devices.
摘要翻译： 在铁电材料样品中引起非线性周期性变化的方法包括将电绝缘掩模施加到样品的表面上，在样品上施加电场以在样品中产生畴反转，并且在非电场样品中除去电场 样品的相反区域仅保留在由掩模覆盖的表面部分下方的样品表面附近。 该方法可以用于设计限于铁电材料的表面区域的亚微米尺寸或更大的精确域周期，使得极化材料可用于制造用于非线性光学应用的平面波导器件。 特别地，亚微米周期可以用于制造一维和二维光子带隙器件。
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公开(公告)号：US08945509B2
公开(公告)日：2015-02-03
申请号：US13215157
申请日：2011-08-22
申请人： Aldrich Lau , Robert Eason , Maxim Brevnov , Handong Li , Kevin Hacker
发明人： Aldrich Lau , Robert Eason , Maxim Brevnov , Handong Li , Kevin Hacker
CPC分类号： G01N33/54326 , C12N1/02 , C12N13/00 , C12Q1/689 , G01N33/54353 , G01N33/56911
摘要： Magnetic beads that include polyvalent ligands comprising various carbohydrates are described. Methods for fabricating such magnetic beads are also provided as well as methods of their use to capture and enrich pathogen cell population for subsequent culture, lysis and identification.
摘要翻译： 描述包括包含各种碳水化合物的多价配体的磁珠。 还提供了制备这种磁珠的方法以及它们用于捕获和富集病原体细胞群以用于随后的培养，裂解和鉴定的方法。
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7.发明申请Synthesis of 2', 3'-Dideoxynucleosides for Automated DNA Synthesis and Pyrophosphorolysis Activated Polymerization 有权用于自动DNA合成和焦磷酸裂解活化聚合的2'，3'-二脱氧核苷的合成公开(公告)号：US20110294122A1
公开(公告)日：2011-12-01
申请号：US13117029
申请日：2011-05-26
CPC分类号： C07H19/16 , C07D239/54 , C07D405/04 , C07D473/18 , C07D473/34 , C07H19/06 , C07H21/00 , C07H21/02 , C07H21/04 , C12Q1/6806
摘要： Methods for preparation of 2′,3′-dideoxynucleotides support structures, such as 2′,3′-dideoxyguanosine, 2′,3′-dideoxyadenosine, and 3′-deoxythymidine support structures are disclosed. Various methods of using such structures are also provided, such as their use for automated DNA synthesis and pyrophosphorolysis activated polymerization.
摘要翻译： 公开了制备2'，3'-二脱氧核苷酸支持结构的方法，例如2'，3'-二脱氧鸟苷，2'，3'-二脱氧腺苷和3'-脱氧胸苷支持结构。 还提供了使用这些结构的各种方法，例如其用于自动DNA合成和焦磷酸裂解活化聚合的用途。
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8.发明申请公开(公告)号：US20060104568A1
公开(公告)日：2006-05-18
申请号：US10522042
申请日：2003-07-21
IPC分类号： G02B6/34
CPC分类号： G02F1/035 , G02B6/122 , G02B6/124 , G02B6/125 , G02B6/13 , G02F1/3558 , G02F2202/13
摘要： A method of inducing refractive index modifications in ferroelectric materials through the application of light in the form of scanned or patterned irradiation, removing the light to leave a permanent refractive index modified structure. This method can be used to design and engineer surface or near-surface structures in the form of waveguides, junctions, splitters and couplers, for application in optical circuitry, integrated optics, and active waveguide devices.
摘要翻译： 通过施加以扫描或图案化照射的形式的光来在铁电材料中引起折射率修饰的方法，去除光以留下永久折射率修饰的结构。 该方法可用于设计和制造波导，结，分离器和耦合器形式的表面或近表面结构，用于光电路，集成光学器件和有源波导器件。
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