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公开(公告)号:US20070095667A1
公开(公告)日:2007-05-03
申请号:US11426222
申请日:2006-06-23
申请人: Aldrich Lau
发明人: Aldrich Lau
CPC分类号: B03C5/026 , B03C5/005 , B03C5/028 , C07K1/26 , G01N27/305
摘要: The present teachings relate to systems and methods for separation of substances such as cells, nucleic acids, and carbon nanotubes. The substances are combined with a separation medium in a liquid sample cavity, for example a microchannel, and transit through the separation by optically activated dielectrophoretic forces. The substances are advantageously labeled and visualized using a microscope and camera.
摘要翻译: 本教导涉及用于分离诸如细胞,核酸和碳纳米管的物质的系统和方法。 这些物质与液体样品腔(例如微通道)中的分离介质组合,并通过光学活化的介电电泳力穿过分离。 使用显微镜和照相机有利地将物质标记和可视化。
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公开(公告)号:US20060160122A1
公开(公告)日:2006-07-20
申请号:US11355872
申请日:2006-02-15
申请人: Michael Harrold , Aldrich Lau , Ben Johnson , Gilbert Amparo , Frank Mercer
发明人: Michael Harrold , Aldrich Lau , Ben Johnson , Gilbert Amparo , Frank Mercer
IPC分类号: C12Q1/68
CPC分类号: C12Q1/6869 , B01D15/34 , B01J13/10 , B01J13/22 , B01J47/018 , B01J47/04 , C12Q1/6806 , C12Q2527/125 , C12Q2565/631 , C12Q2565/125 , C12Q2563/113
摘要: A polyelectrolyte-coated particle, devices for using the particle, methods for using the particle for separating PCR reaction products and/or DNA sequencing reaction products, and compositions for coating the particle are provided.
摘要翻译: 提供聚电解质涂覆的颗粒,使用该颗粒的装置,使用该颗粒分离PCR反应产物和/或DNA测序反应产物的方法,以及用于涂覆颗粒的组合物。
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公开(公告)号:US08945509B2
公开(公告)日:2015-02-03
申请号:US13215157
申请日:2011-08-22
申请人: Aldrich Lau , Robert Eason , Maxim Brevnov , Handong Li , Kevin Hacker
发明人: Aldrich Lau , Robert Eason , Maxim Brevnov , Handong Li , Kevin Hacker
CPC分类号: G01N33/54326 , C12N1/02 , C12N13/00 , C12Q1/689 , G01N33/54353 , G01N33/56911
摘要: Magnetic beads that include polyvalent ligands comprising various carbohydrates are described. Methods for fabricating such magnetic beads are also provided as well as methods of their use to capture and enrich pathogen cell population for subsequent culture, lysis and identification.
摘要翻译: 描述包括包含各种碳水化合物的多价配体的磁珠。 还提供了制备这种磁珠的方法以及它们用于捕获和富集病原体细胞群以用于随后的培养,裂解和鉴定的方法。
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4.
公开(公告)号:US08834698B2
公开(公告)日:2014-09-16
申请号:US12702137
申请日:2010-02-08
申请人: Aldrich Lau , Joon Yang , Huan Phan , Steven Sherwood , Hans Fuernkranz
发明人: Aldrich Lau , Joon Yang , Huan Phan , Steven Sherwood , Hans Fuernkranz
CPC分类号: B03C5/026 , B03C5/005 , B03C5/028 , C07K1/26 , G01N27/305
摘要: A method for sorting cells in a biological sample comprising a first type of cells and a second type of cells may comprise introducing the biological sample into a chamber comprising a first surface and a second surface, wherein the first surface is associated with a transparent electrode and the second surface is associated with a photoconductive portion of an electrode. The method may further comprise moving incident light and the photoconductive portion relative to one another so as to illuminate regions of the photoconductive portion and modulate an electric field in the chamber in proximity to the illuminated regions. The method may further comprise separating the first type of cells from the second type of cells in the chamber via dielectrophoretic movement of the first type of cells and the second type of cells caused by the modulated electric field, wherein a dielectrophoretic characteristic of at least one of the first type of cells and the second type of cells has been modified.
摘要翻译: 用于分选生物样品中包含第一类型细胞和第二类型细胞的细胞的方法可以包括将生物样品引入包含第一表面和第二表面的室中,其中第一表面与透明电极相关联, 第二表面与电极的光电导部分相关联。 该方法还可以包括相对于彼此移动入射光和光电导部分,以照射光电导部分的区域并且调制在邻近照明区域的室中的电场。 该方法还可以包括通过由调制电场引起的第一类型的细胞和第二类型的细胞的介电电泳运动,将第一类型的细胞与腔室中的第二类型的细胞分离,其中至少一种的介电电泳特性 的第一类型的细胞和第二类型的细胞已被修改。
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公开(公告)号:US20070099211A1
公开(公告)日:2007-05-03
申请号:US11488439
申请日:2006-07-17
申请人: Vissarion Aivazachvili , Kristian Scaboo , Aldrich Lau , Konrad Faulstich , Robert Eason , John Van Camp , Timothy Liu
发明人: Vissarion Aivazachvili , Kristian Scaboo , Aldrich Lau , Konrad Faulstich , Robert Eason , John Van Camp , Timothy Liu
CPC分类号: C12Q1/6823 , B01L3/5027 , B01L7/525 , B01L2200/10 , B01L2300/0645 , B01L2300/0681 , B01L2300/1844 , B01L2300/185 , B01L2300/1872 , B01L2300/1894 , B01L2400/0418 , C12Q1/6825 , C12Q2565/607 , C12Q2563/131 , C12Q2565/629 , C12Q2531/113 , C12Q2521/301 , C12Q2523/107
摘要: Methods for detecting a target polynucleotide sequences are provided that utilize a probe having a target-complementary segment and a detectable tag. By cleaving the detectable tab and associating the tag with a tag complement coupled to an electrode, an electrochemical signal can be detected that is related to the presence of the tag:tag complement complex.
摘要翻译: 提供了检测靶多核苷酸序列的方法,其利用具有靶互补区段和可检测标签的探针。 通过切割可检测的标签并将标签与耦合到电极的标签补体相关联,可以检测到与标签:标签补体复合物的存在相关的电化学信号。
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公开(公告)号:US20060093528A1
公开(公告)日:2006-05-04
申请号:US11252821
申请日:2005-10-18
申请人: Debjyoti Banerjee , Konrad Faulstich , Aldrich Lau , Umberto Ulmanella , Jun Xie
发明人: Debjyoti Banerjee , Konrad Faulstich , Aldrich Lau , Umberto Ulmanella , Jun Xie
IPC分类号: B01L3/00
CPC分类号: F16K99/0001 , B01L3/502738 , B01L3/502746 , B01L2200/0621 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/087 , B01L2400/0406 , B01L2400/0415 , B01L2400/0481 , B01L2400/0487 , B01L2400/0655 , B01L2400/0661 , B01L2400/0677 , B01L2400/0683 , B01L2400/082 , B01L2400/084 , B01L2400/086 , F16K99/003 , F16K99/0036 , F16K99/0057 , F16K2099/0084 , Y10S422/901 , Y10T137/0318 , Y10T137/0324 , Y10T137/1624 , Y10T137/1812 , Y10T137/2191 , Y10T137/8376 , Y10T137/8593 , Y10T137/85978 , Y10T436/143333 , Y10T436/2575
摘要: A diagnostic device is provided that includes a plurality of retainment regions, with the retainment regions that are separated by at least one dissolvable barrier. The retainment regions can be interconnected through at least one fluid processing passageway. A retainment region can include a container such as a retainment region, well, chamber, or other receptacle, or a retainment region such as a surface on which the material is retained. The retainment regions can include a reaction retainment region, one or more reagent retainment regions, each containing unreacted reagents, and a sample retainment region. A pressure-actuated valve can be positioned in each fluid processing passageway interconnecting the one or more reagent retainment regions with the respective intermediate retainment regions interposed between each of the one or more reagent retainment regions and the reaction retainment region. The dissolvable barrier can be a fluid flow modulator in the at least one fluid processing passageway.
摘要翻译: 提供了一种诊断装置,其包括多个保持区域,其中保留区域被至少一个可溶解屏障隔开。 保持区域可以通过至少一个流体处理通道相互连接。 保持区域可以包括诸如保持区域,孔,腔室或其它容器的容器,或诸如保持材料的表面的保持区域。 保留区域可以包括反应保留区域,一个或多个试剂保留区域,每个含有未反应的试剂和样品保留区域。 压力致动阀可以定位在每个流体处理通道中,其将一个或多个试剂保留区域与插入在一个或多个试剂保留区域中的每一个和反应保留区域之间的各个中间保留区域互连。 可溶解屏障可以是至少一个流体处理通道中的流体流动调节器。
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7.
公开(公告)号:US20060093526A1
公开(公告)日:2006-05-04
申请号:US11252915
申请日:2005-10-18
申请人: Konrad Faulstich , Aldrich Lau , Debjyoti Banerjee , Umberto Ulmanella , Jun Xie
发明人: Konrad Faulstich , Aldrich Lau , Debjyoti Banerjee , Umberto Ulmanella , Jun Xie
IPC分类号: B01L3/00
CPC分类号: F16K99/0001 , B01L3/502738 , B01L3/502746 , B01L2200/0621 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/087 , B01L2400/0406 , B01L2400/0415 , B01L2400/0481 , B01L2400/0487 , B01L2400/0655 , B01L2400/0661 , B01L2400/0677 , B01L2400/0683 , B01L2400/082 , B01L2400/084 , B01L2400/086 , F16K99/003 , F16K99/0036 , F16K99/0057 , F16K2099/0084 , Y10S422/901 , Y10T137/0318 , Y10T137/0324 , Y10T137/1624 , Y10T137/1812 , Y10T137/2191 , Y10T137/8376 , Y10T137/8593 , Y10T137/85978 , Y10T436/143333 , Y10T436/2575
摘要: A fluid processing device is provided that includes a substrate, a plurality of fluid retainment regions formed in or on the substrate, and a barrier at least partially separating two or more of the fluid retainment regions. The barrier includes a mixture of a sequestering material and a reaction component. The reaction component can be at least one of a reactant, a reagent, a catalyst, an initiator, a promoter, a cofactor, an enzyme, a salt, or a combination thereof. The sequestering material can be a porous material, a dissolvable material, or both.
摘要翻译: 提供了一种流体处理装置,其包括衬底,形成在衬底中或衬底上的多个流体保持区域,以及至少部分地分离两个或更多个流体保持区域的屏障。 屏障包括螯合材料和反应组分的混合物。 反应组分可以是反应物,试剂,催化剂,引发剂,助催化剂,辅因子,酶,盐或其组合中的至少一种。 隔离材料可以是多孔材料,可溶解材料或两者。
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公开(公告)号:US20050208543A1
公开(公告)日:2005-09-22
申请号:US11031983
申请日:2005-01-06
申请人: Charles Vann , Charles Connell , Aldrich Lau , Meng Taing , Steven Menchen
发明人: Charles Vann , Charles Connell , Aldrich Lau , Meng Taing , Steven Menchen
IPC分类号: C12Q1/68 , C40B20/04 , C40B70/00 , G01N33/53 , G01N33/533 , G01N33/543 , G01N33/58
CPC分类号: B82Y5/00 , C12Q2563/155 , G01N33/54346 , G01N33/587
摘要: Beads coded with phosphor particles and methods of making and using them are provided.
摘要翻译: 提供用磷光体颗粒编码的珠子,并制作和使用它们的方法。
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公开(公告)号:US20050181378A1
公开(公告)日:2005-08-18
申请号:US10780963
申请日:2004-02-18
申请人: Michael Harrold , Aldrich Lau
发明人: Michael Harrold , Aldrich Lau
CPC分类号: C12N15/101 , B01D15/34 , B01J20/3293 , B01J43/00 , B01J47/018 , B01J47/04 , Y10T428/29
摘要: A polyelectrolyte-coated particle, devices for using the particle, methods for using the particle for separating PCR reaction products and/or DNA sequencing reaction products, and compositions for coating the particle are provided.
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公开(公告)号:US20120045767A1
公开(公告)日:2012-02-23
申请号:US13215157
申请日:2011-08-22
申请人: Aldrich LAU , Robert Eason , Maxim Brevnov , Handong Li , Kevin Hacker
发明人: Aldrich LAU , Robert Eason , Maxim Brevnov , Handong Li , Kevin Hacker
CPC分类号: G01N33/54326 , C12N1/02 , C12N13/00 , C12Q1/689 , G01N33/54353 , G01N33/56911
摘要: Magnetic beads that include polyvalent ligands comprising various carbohydrates are described. Methods for fabricating such magnetic beads are also provided as well as methods of their use to capture and enrich pathogen cell population for subsequent culture, lysis and identification.
摘要翻译: 描述包括包含各种碳水化合物的多价配体的磁珠。 还提供了制备这种磁珠的方法以及它们用于捕获和富集病原体细胞群以用于随后的培养,裂解和鉴定的方法。
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