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公开(公告)号：US08153085B2
公开(公告)日：2012-04-10
申请号：US12949981
申请日：2010-11-19
申请人： Kyu-youn Hwang , Joon-ho Kim , Chin-sung Park , Sung-young Jeong
发明人： Kyu-youn Hwang , Joon-ho Kim , Chin-sung Park , Sung-young Jeong
IPC分类号： B01L3/00
CPC分类号： B01L3/502707 , B01L3/502753 , B01L2200/12 , B01L2300/16 , Y10T428/14
摘要： Provided are a microfluidic chip and a method of fabricating the same. The microfluidic chip includes: a lower substrate; an upper substrate formed of a silicone resin, wherein the lower substrate and the upper substrate, bonded together, provide a channel through which a fluid can flow and a chamber to receive the fluid; and an organic thin film formed on the upper surface of the lower substrate except for portions on which the lower substrate and the upper substrate are attached to each other.
摘要翻译： 提供了一种微流控芯片及其制造方法。 微流体芯片包括：下基板; 由硅树脂形成的上基板，其中所述下基板和所述上基板接合在一起，提供流体可以流过的通道和容纳所述流体的室; 以及形成在下基板的上表面上的有机薄膜，除了下基板和上基板彼此附接的部分之外。
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2.发明申请METHOD OF PRODUCING SOLID SUPPORT FOR BIOLOGICAL ANALYSIS USING PLASTIC MATERIAL 审中-公开使用塑料材料生产生物分析的固体支持方法公开(公告)号：US20090098291A1
公开(公告)日：2009-04-16
申请号：US12100700
申请日：2008-04-10
申请人： Kyu-youn HWANG , Joon-ho Kim , Sung-young Jeong , Chin-sung Park
发明人： Kyu-youn HWANG , Joon-ho Kim , Sung-young Jeong , Chin-sung Park
CPC分类号： G01N33/54353 , C23C14/025 , C23C14/028 , C23C14/10 , G01N33/553
摘要： The present invention provides a method of manufacturing a solid support for biological analysis using a plastic material, the method including: depositing a metal film on a plastic substrate on which a microstructure is formed; depositing an inorganic oxide on the metal film; and anchoring a compound with an amino functional group or a compound with a water contact angle of 70 to 95 degrees on the inorganic oxide.
摘要翻译： 本发明提供一种使用塑料材料制造用于生物分析的固体支持物的方法，所述方法包括：在形成微结构的塑料基板上沉积金属膜; 在金属膜上沉积无机氧化物; 并且将具有氨基官能团的化合物或具有70至95度的水接触角的化合物锚定在无机氧化物上。
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公开(公告)号：US20080305011A1
公开(公告)日：2008-12-11
申请号：US11934811
申请日：2007-11-05
申请人： Kyu-youn HWANG , Joon-ho Kim , Chin-sung Park , Sung-young Jeong
发明人： Kyu-youn HWANG , Joon-ho Kim , Chin-sung Park , Sung-young Jeong
CPC分类号： B01L3/502707 , B01L3/502753 , B01L2200/12 , B01L2300/16 , Y10T428/14
摘要： Provided are a microfluidic chip and a method of fabricating the same. The microfluidic chip includes: a lower substrate; an upper substrate formed of a silicone resin, wherein the lower substrate and the upper substrate, bonded together, provide a channel through which a fluid can flow and a chamber to receive the fluid; and an organic thin film formed on the upper surface of the lower substrate except for portions on which the lower substrate and the upper substrate are attached to each other.
摘要翻译： 提供了一种微流控芯片及其制造方法。 微流体芯片包括：下基板; 由硅树脂形成的上基板，其中所述下基板和所述上基板接合在一起，提供流体可以流过的通道和容纳所述流体的室; 以及形成在下基板的上表面上的有机薄膜，除了下基板和上基板彼此附接的部分之外。
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公开(公告)号：US07858042B2
公开(公告)日：2010-12-28
申请号：US11934811
申请日：2007-11-05
申请人： Kyu-youn Hwang , Joon-ho Kim , Chin-sung Park , Sung-young Jeong
发明人： Kyu-youn Hwang , Joon-ho Kim , Chin-sung Park , Sung-young Jeong
IPC分类号： B01N3/00
CPC分类号： B01L3/502707 , B01L3/502753 , B01L2200/12 , B01L2300/16 , Y10T428/14
摘要： Provided are a microfluidic chip and a method of fabricating the same. The microfluidic chip includes: a lower substrate; an upper substrate formed of a silicone resin, wherein the lower substrate and the upper substrate, bonded together, provide a channel through which a fluid can flow and a chamber to receive the fluid; and an organic thin film formed on the upper surface of the lower substrate except for portions on which the lower substrate and the upper substrate are attached to each other.
摘要翻译： 提供了一种微流控芯片及其制造方法。 微流体芯片包括：下基板; 由硅树脂形成的上基板，其中所述下基板和所述上基板接合在一起，提供流体可以流过的通道和容纳所述流体的室; 以及形成在下基板的上表面上的有机薄膜，除了下基板和上基板彼此附接的部分之外。
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公开(公告)号：US08859272B2
公开(公告)日：2014-10-14
申请号：US13275871
申请日：2011-10-18
申请人： Kyu-youn Hwang , Joon-ho Kim , Sung-hong Kwon , Chin-sung Park , Hee-kyun Lim , Sun-ok Jung , Won-jong Jung
发明人： Kyu-youn Hwang , Joon-ho Kim , Sung-hong Kwon , Chin-sung Park , Hee-kyun Lim , Sun-ok Jung , Won-jong Jung
CPC分类号： C12Q1/6806 , C12M1/33 , C12M47/06
摘要： A micro-device for disrupting cells includes a first chamber in which the cells are disrupted, a second chamber which is pressurized and depressurized, a flexible membrane which separates the first chamber and the second chamber and is vibrated by pressuring and depressurizing the second chamber, and a micro-unit confined in the first chamber, where the micro-unit disrupts the cells in the first chamber
摘要翻译： 用于破坏电池的微型装置包括其中电池被破坏的第一室，被加压和减压的第二室，分离第一室和第二室的柔性膜，并且通过对第二室进行加压和减压而振动， 以及限制在第一室中的微单元，其中微单元破坏第一室中的单元
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公开(公告)号：US20060234379A1
公开(公告)日：2006-10-19
申请号：US11405106
申请日：2006-04-17
申请人： Hee-kyun Lim , Kyu-youn Hwang , Joon-ho Kim , Kak Namkoong , Chin-sung Park
发明人： Hee-kyun Lim , Kyu-youn Hwang , Joon-ho Kim , Kak Namkoong , Chin-sung Park
CPC分类号： B01L3/502753 , B01L3/502746 , B01L2300/0681 , B01L2400/086 , B82Y30/00 , B82Y40/00 , C12M47/02
摘要： Provided is a method of separating cells using a hydrophobic solid support. The method comprises contacting a solution containing cells with a hydrophobic solid support having a water contact angle between 70 and 90 degrees. By allowing cells to be adsorbed to the hydrophobic solid substrate, the cells can be separated. Thus, the cell separation efficiency can be rapidly and simply increased.
摘要翻译： 提供了使用疏水性固体支持物分离细胞的方法。 该方法包括将含有细胞的溶液与70-90度的水接触角的疏水性固体支持物接触。 通过使细胞被吸附到疏水性固体底物上，可以分离细胞。 因此，可以快速且简单地增加细胞分离效率。
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公开(公告)号：US08715965B2
公开(公告)日：2014-05-06
申请号：US11746722
申请日：2007-05-10
申请人： Hun-joo Lee , Sung-young Jeong , Joon-ho Kim , Kyu-youn Hwang
发明人： Hun-joo Lee , Sung-young Jeong , Joon-ho Kim , Kyu-youn Hwang
CPC分类号： B01D61/422 , B01D2325/20 , C12Q1/6806 , C12Q2565/629
摘要： A method of amplifying nucleic acid from blood, the method including performing an electrodialysis on a blood sample to reduce the ionic strength of the sample, and performing a Polymerase Chain Reaction (“PCR”) using the blood sample on which the electrodialysis is performed as a template.
摘要翻译： 一种从血液中扩增核酸的方法，所述方法包括对血液样品进行电渗析以降低样品的离子强度，并使用进行电渗析的血液样品进行聚合酶链式反应（“PCR”）， 一个模板
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8.发明授权Method of separating microorganism using nonplanar solid substrate and device for separating microorganism using the same 有权使用非平面固体底物分离微生物的方法和使用其分离微生物的装置公开(公告)号：US08158411B2
公开(公告)日：2012-04-17
申请号：US11841117
申请日：2007-08-20
申请人： Kyu-youn Hwang , Sung-young Jeong , Joon-ho Kim
发明人： Kyu-youn Hwang , Sung-young Jeong , Joon-ho Kim
IPC分类号： C12M1/00
摘要： Provided is a method of separating microorganisms from a sample including contacting the sample containing microorganisms with an inorganic ion exchange material such that the sample reacts with the inorganic ion exchange material, and contacting the reacted sample with a means for capturing microorganisms.
摘要翻译： 提供了从样品中分离微生物的方法，包括使含有微生物的样品与无机离子交换材料接触，使得样品与无机离子交换材料反应，并使反应的样品与捕获微生物的方法接触。
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9.发明授权Method of isolating nucleic acid using material positively charged at first pH and containing amino group and carboxyl group 有权使用在第一个pH下带正电并含有氨基和羧基的物质分离核酸的方法公开(公告)号：US07402390B2
公开(公告)日：2008-07-22
申请号：US11317949
申请日：2005-12-23
申请人： Kyu-youn Hwang , Joon-ho Kim , Chang-eun Yoo , Hun-joo Lee , Hee-kyun Lim , Sung-young Jeong , Jeo-young Shim
发明人： Kyu-youn Hwang , Joon-ho Kim , Chang-eun Yoo , Hun-joo Lee , Hee-kyun Lim , Sung-young Jeong , Jeo-young Shim
CPC分类号： C12N15/1006 , B01D15/361 , B01D15/364 , B01J20/3285 , B01J43/00 , C12Q1/6806 , C12Q2527/119 , C12Q2523/308
摘要： A method of isolating nucleic acid from a sample containing nucleic acid is provided. The method includes contacting the sample with a bifunctional material that contains an amino group and a carboxyl group and is positively charged at a first pH to allow binding of the nucleic acid to the bifunctional material; and extracting the nucleic acid at a second pH higher than the first pH from the complex.
摘要翻译： 提供了从含有核酸的样品中分离核酸的方法。 该方法包括使样品与含有氨基和羧基的双官能材料接触并在第一pH下带正电荷以允许核酸与双功能材料的结合; 以及从所述络合物中提取比所述第一pH高的第二pH的所述核酸。
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10.发明申请METHOD AND DEVICE FOR SEPARATING CELLS FROM A SAMPLE USING A NONPLANAR SOLID SUBSTRATE 审中-公开使用非固体基质从样品分离细胞的方法和装置公开(公告)号：US20080044884A1
公开(公告)日：2008-02-21
申请号：US11841089
申请日：2007-08-20
申请人： Kyu-youn Hwang , Sung-young Jeong , Joon-ho Kim , Hun-joo Lee , Jung-im Han
发明人： Kyu-youn Hwang , Sung-young Jeong , Joon-ho Kim , Hun-joo Lee , Jung-im Han
CPC分类号： C12N15/1006 , B01L7/52 , B01L2300/0636 , C12N2533/30 , C12Q1/6806
摘要： Provided is a method and device for separating a cell from a sample. The method comprises contacting a nonplanar solid substrate with a cell-containing sample in a liquid medium having a pH of 3.0 to 6.0.
摘要翻译： 提供了用于从样品中分离细胞的方法和装置。 该方法包括使非平面固体基质与含有细胞的样品在pH为3.0至6.0的液体培养基中接触。
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