公开(公告)号：US11493526B2

公开(公告)日：2022-11-08

申请号：US16852830

申请日：2020-04-20

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Eliodor Ghenciu ,  Theodore E. Straub ,  Evan W. Foster ,  Michael C. Pallas

IPC: G01N35/10 ,  C40B60/14 ,  B01L3/00 ,  B01J19/00

Abstract: An apparatus for biological reactions is provided. The apparatus includes a substrate and a plurality of reaction sites within the substrate. A surface of the substrate is configured to have a first hydrophilicity and each surface of the plurality of reaction sites is configured to have a second hydrophilicity to load a substantial number of reaction sites with a sample volume. The sample volume of each loaded reaction site is substantially confined to its respective reaction site. The sample volume is configured to undergo a biological reaction within the reaction site.

公开(公告)号：US20150080247A1

公开(公告)日：2015-03-19

申请号：US14385730

申请日：2013-03-15

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Michael C. Pallas ,  James C. Nurse ,  Gary Lim ,  Theodore E. Straub ,  Eliodor Ghenciu ,  Evan W. Foster ,  Jorge Fonseca ,  Kevin Maher

IPC: B01L3/00 ,  C12Q1/68

CPC classification number: B01L3/50857 ,  B01L3/50 ,  B01L3/50851 ,  B01L7/52 ,  B01L2200/0642 ,  B01L2200/142 ,  B01L2300/046 ,  B01L2300/0654 ,  B01L2300/0829 ,  B01L2300/0893 ,  B01L2300/0896 ,  B01L2300/161 ,  C12Q1/6806 ,  C12Q1/686 ,  G03F7/2002

Abstract: A system for performing biological reactions is provided. The system includes a chip including a substrate and a plurality of reaction sites. The plurality of reaction sites are each configured to include a liquid sample of at most one nanoliter. Further, the system includes a control system configured to initiate biological reactions within the liquid samples. The system further includes a detection system configured to detect biological reactions on the chip. According to various embodiments, the chip includes at least 20000 reaction sites. In other embodiments, the chip includes at least 30000 reaction sites.

Abstract translation: 提供了用于进行生物反应的系统。 该系统包括包括基底和多个反应位点的芯片。 多个反应位点各自被配置为包括至多一个纳升级的液体样品。 此外，该系统包括被配置为启动液体样品内的生物反应的控制系统。 该系统还包括被配置为检测芯片上的生物反应的检测系统。 根据各种实施方案，芯片包括至少20000个反应位点。 在其它实施方案中，芯片包括至少30000个反应位点。

公开(公告)号：US20150072897A1

公开(公告)日：2015-03-12

申请号：US14385735

申请日：2013-03-15

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Michael C. Pallas ,  James C. Nurse ,  Gary Lim ,  Theodore E. Straub ,  Evan W. Foster

IPC: G01N35/10 ,  B01J19/00

CPC classification number: G01N35/1002 ,  B01J19/0046 ,  B01J2219/00286 ,  B01J2219/00317 ,  B01J2219/00351 ,  B01J2219/0038 ,  B01J2219/00599 ,  B01J2219/00619 ,  B01J2219/00637 ,  B01L3/5088 ,  B01L2200/0642 ,  B01L2200/12 ,  B01L2300/0893 ,  B01L2300/0896 ,  B01L2300/161 ,  B01L2300/165 ,  B01L2400/0406 ,  C40B60/14

Abstract: A sample loader for loading a liquid sample into a plurality of reaction sites within a substrate is provided. The sample loader includes a first blade, and a second blade coupled to the first blade. The sample loader further comprises a flow path between the first blade and second blade configured to dispense a liquid sample to a substrate including a plurality of reaction sites. Further, in various embodiments the liquid sample has an advancing contact angle of 85+/−15 degrees with the first and second blade. Furthermore, loading of the liquid sample dispensed from the flow path to the plurality of reaction sites may be based on capillary action.

Abstract translation: 提供了一种用于将液体样品加载到基底内的多个反应位点的样品装载器。 样品装载器包括第一叶片和联接到第一叶片的第二叶片。 样品装载器还包括在第一叶片和第二叶片之间的流动路径，其构造成将液体样品分配到包括多个反应位点的基底。 此外，在各种实施例中，液体样品与第一和第二叶片具有85 +/- 15度的前进接触角。 此外，从流动路径分配到多个反应位点的液体样品的装载可以基于毛细作用。

公开(公告)号：US20150051118A1

公开(公告)日：2015-02-19

申请号：US14385740

申请日：2013-03-15

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Eliodor Ghenciu ,  Theodore E. Straub ,  Evan W. Foster ,  Michael C. Pallas

IPC: B01L3/00

Abstract: An apparatus for biological reactions is provided. The apparatus includes a substrate and a plurality of reaction sites within the substrate. A surface of the substrate is configured to have a first hydrophilicity and each surface of the plurality of reaction sites is configured to have a second hydrophilicity to load a substantial number of reaction sites with a sample volume. The sample volume of each loaded reaction site is substantially confined to its respective reaction site. The sample volume is configured to undergo a biological reaction within the reaction site.

Abstract translation: 提供了用于生物反应的装置。 该装置包括衬底和衬底内的多个反应位点。 衬底的表面被配置为具有第一亲水性，并且多个反应位点的每个表面被配置为具有第二亲水性，以加载具有样品体积的大量反应位点。 每个加载的反应位点的样品体积基本上限于其各自的反应位点。 样品体积被配置为在反应位点内进行生物反应。