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    Systems and methods for performing digital PCR
    3.
    发明授权
    Systems and methods for performing digital PCR 有权

    公开(公告)号：US11085074B2

    公开(公告)日：2021-08-10

    申请号：US15280160

    申请日：2016-09-29

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： David N. Keys ,  Nivedita Sumi Majumdar ,  Theodore E. Straub

    IPC: C12Q1/686 ,  C12Q1/6851 ,  B01L7/00

    Abstract: Systems and methods are described for quantifying a target nucleic acid. A sample comprising a target nucleic acid is segregated into a first plurality of the reaction volumes containing at least one target nucleic acid molecule and a second plurality of the reaction volumes containing no target nucleic acid molecules. The reaction volumes are subjected to an amplification assay, wherein the amplification assay is configured to amplify the target nucleic acid. An indicator of amplification is detected or measured in at least some of the plurality of reaction volumes. The target nucleic acid is quantified based on the detection or measurement. After discontinuing the amplification assay, the plurality of reaction volumes may be heated and changes in the indicators of amplification of two or more of the at least some of the reaction volumes may be detected or measured.

    Visualization Tools For Digital PCR Data
    4.
    发明申请
    Visualization Tools For Digital PCR Data 审中-公开
    数字PCR数据可视化工具

    公开(公告)号：US20150269756A1

    公开(公告)日：2015-09-24

    申请号：US14441500

    申请日：2013-11-07

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Harrison Leong ,  Nivedita Sumi Majumdar ,  Jeffrey A. Marks ,  Theodore E. Straub ,  Ryan J. Talbot ,  Kevin S. Bodner ,  David Hoard

    IPC: G06T11/20

    CPC classification number: G06T11/206 ,  G06T11/203 ,  G16B45/00

    Abstract: A method for generating a data visualization is provided. The method includes displaying a representation of a portion of detected data from a substrate to a user. The method further includes generating a data quality value for the portion of detected data and displaying, along with the representation of the portion of detected data, an indication of data quality value for the portion of detected data. The method further includes selecting, by the user, a quality value threshold, and displaying an adjusted indication of data quality value for the portion of detected data meeting the quality value threshold.

    Abstract translation: 提供了一种用于生成数据可视化的方法。 该方法包括将检测到的数据的一部分从衬底显示给用户。 该方法还包括为检测到的数据的部分生成数据质量值，以及与检测到的数据的部分的表示一起显示检测到的数据的部分的数据质量值的指示。 该方法还包括由用户选择质量值阈值，并且显示对于满足质量值阈值的检测数据的部分的数据质量值的调整指示。

    Methods and systems for visualizing data quality
    5.
    发明授权
    Methods and systems for visualizing data quality 有权

    公开(公告)号：US11461338B2

    公开(公告)日：2022-10-04

    申请号：US14392323

    申请日：2014-06-27

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Nivedita Sumi Majumdar ,  Harrison Leong ,  Theodore E. Straub ,  David Hoard

    IPC: G06F16/2457 ,  G06F16/248 ,  G16B45/00

    Abstract: A method for generating a data visualization is provided. The method includes receiving a plurality of data points related to fluorescent emissions values from a plurality of reaction sites. The fluorescent emission values include information for a first type of dye and a second type of dye. The method further includes displaying a first portion of the plurality of data points related to the first type of dye in a representation of location of the plurality of reaction sites, and displaying a second portion of the plurality of data points related to the second type of dye in the representation. The method further includes displaying the first portion of the plurality of data points in a scatter plot display. The scatter plot shows fluorescent values related to the first dye on the y-axis and fluorescent values related to the second dye on the x-axis. The method includes displaying the second portion of the plurality of data points in the scatter plot display.

    SYSTEMS AND METHODS FOR PERFORMING DIGITAL PCR
    6.
    发明申请
    SYSTEMS AND METHODS FOR PERFORMING DIGITAL PCR 有权

    公开(公告)号：US20220119859A1

    公开(公告)日：2022-04-21

    申请号：US17385853

    申请日：2021-07-26

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： David N. Keys ,  Nivedita Sumi Majumdar ,  Theodore E. Straub

    IPC: C12Q1/686 ,  C12Q1/6851

    Abstract: Systems and methods are described for quantifying a target nucleic acid. A sample comprising a target nucleic acid is segregated into a first plurality of the reaction volumes containing at least one target nucleic acid molecule and a second plurality of the reaction volumes containing no target nucleic acid molecules. The reaction volumes are subjected to an amplification assay, wherein the amplification assay is configured to amplify the target nucleic acid. An indicator of amplification is detected or measured in at least some of the plurality of reaction volumes. The target nucleic acid is quantified based on the detection or measurement. After discontinuing the amplification assay, the plurality of reaction volumes may be heated and changes in the indicators of amplification of two or more of the at least some of the reaction volumes may be detected or measured.

    Methods and Systems for Visualizing Data Quality
    10.
    发明申请
    Methods and Systems for Visualizing Data Quality 审中-公开
    数据质量可视化方法与系统

    公开(公告)号：US20160275149A1

    公开(公告)日：2016-09-22

    申请号：US14392323

    申请日：2014-06-27

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Nivedita Sumi Majumdar ,  Harrison Leong ,  Theodore E. Straub ,  David Hoard

    IPC: G06F17/30

    CPC classification number: G06F16/24575 ,  G06F16/248 ,  G16B45/00

    Abstract: A method for generating a data visualization is provided. The method includes receiving a plurality of data points related to fluorescent emissions values from a plurality of reaction sites. The fluorescent emission values include information for a first type of dye and a second type of dye. The method further includes displaying a first portion of the plurality of data points related to the first type of dye in a representation of location of the plurality of reaction sites, and displaying a second portion of the plurality of data points related to the second type of dye in the representation. The method further includes displaying the first portion of the plurality of data points in a scatter plot display. The scatter plot shows fluorescent values related to the first dye on the y-axis and fluorescent values related to the second dye on the x-axis. The method includes displaying the second portion of the plurality of data points in the scatter plot display.

    Abstract translation: 提供了一种用于生成数据可视化的方法。 该方法包括从多个反应位点接收与荧光发射值有关的多个数据点。 荧光发射值包括第一类染料和第二类染料的信息。 该方法还包括在多个反应位置的位置的表示中显示与第一类型的染料相关的多个数据点的第一部分，以及显示与第二类型的染色相关的多个数据点的第二部分 染料代表。 该方法还包括在散点图显示中显示多个数据点的第一部分。 散点图显示与y轴上的第一染料相关的荧光值和与x轴上的第二染料相关的荧光值。 该方法包括在散点图显示中显示多个数据点的第二部分。

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