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    SYSTEM FOR RAPID HIGH-RESOLUTION GEL ELECTROPHORESIS
    3.
    发明申请
    SYSTEM FOR RAPID HIGH-RESOLUTION GEL ELECTROPHORESIS 审中-公开

    公开(公告)号：US20190391113A1

    公开(公告)日：2019-12-26

    申请号：US16551364

    申请日：2019-08-26

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Timothy UPDYKE ,  Jennifer MILLER

    IPC: G01N27/447

    Abstract: Electrophoretic systems, formulations and methods are described which allow a user to perform electrophoresis experiments under conditions of high voltage and with reduced run time. An electrophoretic system, formulation or method may be run at 50% higher field strength than comparable systems already in use in the art. The presently described systems and formulations may be run at voltages above 225 V, above 250 V, above 275 V, above 300 V, above 325 V or above 350 V. The time required for performing an electrophoresis experiment may be reduced to less than about 30 minutes, less than about 20 minutes, less than about 15 minutes or less than about 12 minutes.

    LIQUID PROTEIN MARKERS FOR NATIVE GEL ELECTROPHORESIS
    5.
    发明申请
    LIQUID PROTEIN MARKERS FOR NATIVE GEL ELECTROPHORESIS 审中-公开
    液体蛋白标记用于原始凝胶电泳

    公开(公告)号：US20150204815A1

    公开(公告)日：2015-07-23

    申请号：US14516315

    申请日：2014-10-16

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Thomas BEARDSLEE ,  Roumen BOGOEV ,  Cinnamon BOLZ ,  Regina ROONEY ,  Timothy UPDYKE

    IPC: G01N27/447

    CPC classification number: G01N27/44704 ,  C07K1/16 ,  C07K1/26

    Abstract: Marker sets are provided for use on nondenaturing gels. The protein molecular weight markers are provided in liquid form, and are stable in liquid form for at least two months at 4 degrees C. and at least one year at −20 degrees C. Methods of using the markers and kits containing stable native protein markers in liquid form for determining molecular mass of proteins using electrophoresis are also provided. Furthermore, methods for generating revenue by selling the liquid molecular weight markers, are provided.

    Abstract translation: 标记组提供用于非变性凝胶。 蛋白质分子量标记物以液体形式提供，并且在4℃下以液体形式稳定至少两个月，在-20℃下至少一年。使用含有稳定天然蛋白质标记物的标记物和试剂盒的方法 还提供用于使用电泳测定蛋白质分子量的液体形式。 此外，提供了通过销售液体分子量标记来产生收入的方法。

    GEL ELECTROPHORESIS, IMAGINE, AND ANALYSIS METHODS, DEVICES, SYSTEMS, AND MATERIALS
    6.
    发明申请
    GEL ELECTROPHORESIS, IMAGINE, AND ANALYSIS METHODS, DEVICES, SYSTEMS, AND MATERIALS 有权
    凝胶电泳，成像和分析方法，器件，系统和材料

    公开(公告)号：US20130175172A1

    公开(公告)日：2013-07-11

    申请号：US13717650

    申请日：2012-12-17

    Applicant: Life Technologies Corporation

    Inventor： Timothy UPDYKE ,  Paul Predki ,  Evangeline Gonzalez ,  Randall Lowe ,  Tad Simons

    IPC: G01N27/447

    CPC classification number: G01N27/44721 ,  G01N27/44778

    Abstract: The present teachings provide methods, devices, systems, and materials for performing electrophoresis in an automated fashion. The electrophoresis system may simultaneously image gel during an electrophoresis run. In some embodiments, the electrophoresis system may analyze an imaged gel during or after electrophoresis. The device may comprise a gel processing system, a gel illumination system, an image capture system, and an image analysis all housed within a housing.

    Abstract translation: 本教导提供了以自动方式进行电泳的方法，装置，系统和材料。 电泳系统可以在电泳过程中同时成像凝胶。 在一些实施方案中，电泳系统可以在电泳期间或之后分析成像的凝胶。 该装置可以包括凝胶加工系统，凝胶照明系统，图像捕获系统和全部容纳在壳体内的图像分析。

    GEL ELECTROPHORESIS, IMAGING, AND ANALYSIS METHODS, DEVICES, SYSTEMS, AND MATERIALS
    7.
    发明申请
    GEL ELECTROPHORESIS, IMAGING, AND ANALYSIS METHODS, DEVICES, SYSTEMS, AND MATERIALS 审中-公开
    凝胶电泳，成像和分析方法，设备，系统和材料

    公开(公告)号：US20160061774A1

    公开(公告)日：2016-03-03

    申请号：US14817143

    申请日：2015-08-03

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Timothy UPDYKE ,  Paul PREDKI ,  Evangeline GONZALEZ ,  Randall LOWE ,  Tad SIMONS

    IPC: G01N27/447

    CPC classification number: G01N27/44721 ,  G01N27/44778

    Abstract: The present teachings provide methods, devices, systems, and materials for performing electrophoresis in an automated fashion. The electrophoresis system may simultaneously image gel during an electrophoresis run. In some embodiments, the electrophoresis system may analyze an imaged gel during or after electrophoresis. The device may comprise a gel processing system, a gel illumination system, an image capture system, and an image analysis all housed within a housing.

    Abstract translation: 本教导提供了以自动方式进行电泳的方法，装置，系统和材料。 电泳系统可以在电泳过程中同时成像凝胶。 在一些实施方案中，电泳系统可以在电泳期间或之后分析成像的凝胶。 该装置可以包括凝胶加工系统，凝胶照明系统，图像捕获系统和全部容纳在壳体内的图像分析。

    SYSTEM FOR RAPID HIGH-RESOLUTION GEL ELECTROPHORESIS
    8.
    发明申请
    SYSTEM FOR RAPID HIGH-RESOLUTION GEL ELECTROPHORESIS 审中-公开
    快速高分辨率凝胶电泳系统

    公开(公告)号：US20140061050A1

    公开(公告)日：2014-03-06

    申请号：US13951378

    申请日：2013-07-25

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Timothy UPDYKE ,  Jennifer C. MILLER

    IPC: G01N27/447

    CPC classification number: G01N27/44747 ,  G01N33/561

    Abstract: Electrophoretic systems, formulations and methods are described which allow a user to perform electrophoresis experiments under conditions of high voltage and with reduced run time. An electrophoretic system, formulation or method may be run at 50% higher field strength than comparable systems already in use in the art. The presently described systems and formulations may be run at voltages above 225 V, above 250 V, above 275 V, above 300 V, above 325 V or above 350 V. The time required for performing an electrophoresis experiment may be reduced to less than about 30 minutes, less than about 20 minutes, less than about 15 minutes or less than about 12 minutes.

    Abstract translation: 描述了电泳系统，配方和方法，其允许用户在高电压和减少运行时间的条件下进行电泳实验。 电泳系统，制剂或方法可以比现有技术中已经使用的可比系统高50％的场强。 目前描述的系统和制剂可以在高于225V，高于250V，高于275V，高于300V，高于325V或高于350V的电压下运行。进行电泳实验所需的时间可以减少到小于约 30分钟，小于约20分钟，小于约15分钟或小于约12分钟。

    COMPOSITIONS AND METHODS FOR IMPROVING RESOLUTION OF BIOMOLECULES SEPARATED ON POLYACRYLAMIDE GELS
    9.
    发明申请
    COMPOSITIONS AND METHODS FOR IMPROVING RESOLUTION OF BIOMOLECULES SEPARATED ON POLYACRYLAMIDE GELS 有权
    用于改善分离在聚丙烯酰胺凝胶上的生物分子分解的组合物和方法

    公开(公告)号：US20140034500A1

    公开(公告)日：2014-02-06

    申请号：US13928137

    申请日：2013-06-26

    Applicant: Life Technologies Corporation

    Inventor： Timothy UPDYKE ,  Thomas BEARDSLEE

    IPC: G01N27/447

    CPC classification number: G01N27/44756 ,  C07K1/26 ,  C08J3/075 ,  C08J2333/26 ,  C08K5/0008 ,  C08L33/26 ,  G01N27/44747

    Abstract: Gels, such as polyacrylamide gels, are provided that include linear polyacrylamide in the stacking gel. Native gels that include linear polyacrylamide in the stacker can be used to separate biomolecular complexes, such as protein complexes. Gel cassettes in which the gap width between front and back plates does not vary by more than 5% at the upper edge of the cassette are also provided. The gel cassettes can be used for electrophoretic separation of proteins and protein complexes on native gels, such as native gels that include linear polyacrylamide in the stacker. The native gels can have multiple wells for electrophoresing at least one sample and/or at least one molecular weight standard.

    Abstract translation: 提供凝胶，如聚丙烯酰胺凝胶，其包括在聚集凝胶中的线性聚丙烯酰胺。 在堆垛机中包括线性聚丙烯酰胺的天然凝胶可用于分离生物分子复合物，例如蛋白质复合物。 还提供了其中前板和后板之间的间隙宽度在盒的上边缘处不变化大于5％的凝胶盒。 凝胶盒可以用于天然凝胶上的蛋白质和蛋白质复合物的电泳分离，例如在堆积器中包括线性聚丙烯酰胺的天然凝胶。 天然凝胶可以具有多个用于电泳至少一个样品和/或至少一个分子量标准的孔。

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