公开(公告)号：US09399986B2

公开(公告)日：2016-07-26

申请号：US13599131

申请日：2012-08-30

Applicant: John Richard Nelson ,  Li Zhu ,  Xiaohui Chen ,  Christopher Michael Puleo ,  Patrick McCoy Spooner

Inventor： John Richard Nelson ,  Li Zhu ,  Xiaohui Chen ,  Christopher Michael Puleo ,  Patrick McCoy Spooner

IPC: C07H21/00 ,  C07H21/02 ,  C07H21/04 ,  B01D11/02 ,  F04B19/00 ,  F04B19/04 ,  F04B19/20

CPC classification number: F04B19/006 ,  F04B19/04 ,  F04B19/20

Abstract: A device, a system, and a method for isolating biomolecules from biological materials are provided. The device comprises a quartz-based solid phase extraction matrix comprising one or more reagents impregnated therein; and an electroosmotic pump (EOP) operationally coupled to the quartz-based solid phase extraction matrix to elute the nucleic acids, wherein the EOP comprises a plurality of electroosmotic membranes comprising one or more positive electroosmotic membranes and one or more negative electroosmotic membranes disposed alternatively and a plurality of electrodes comprising one or more cathodes and one or more anodes, wherein at least one cathode is disposed on one side of one of the membranes and at least one anode is disposed on another side of that membrane and at least one cathode or anode is disposed between a positive electroosmotic membrane and a negative electroosmotic membrane.

Abstract translation: 提供了一种从生物材料中分离生物分子的装置，系统和方法。 该装置包括石英基固相萃取基质，其包含一种或多种浸渍在其中的试剂; 和电渗泵（EOP），其可操作地耦合到所述基于石英的固相萃取基质以洗脱所述核酸，其中所述EOP包括多个电渗膜，所述电渗膜包含一个或多个正电渗透膜和一个或多个负电渗渗膜， 包括一个或多个阴极和一个或多个阳极的多个电极，其中至少一个阴极设置在一个膜的一侧上，并且至少一个阳极设置在该膜的另一侧上，并且至少一个阴极或阳极 设置在正电渗透膜和负电渗膜之间。

公开(公告)号：US20150259671A1

公开(公告)日：2015-09-17

申请号：US13562947

申请日：2012-07-31

Applicant: Christopher Michael Puleo ,  Ralf Lenigk ,  John Richard Nelson ,  Xiaohui Chen ,  Li Zhu ,  Erin Jean Finehout ,  Patrick McCoy Spooner

Inventor： Christopher Michael Puleo ,  Ralf Lenigk ,  John Richard Nelson ,  Xiaohui Chen ,  Li Zhu ,  Erin Jean Finehout ,  Patrick McCoy Spooner

IPC: C12N15/10

CPC classification number: C12N15/101 ,  B01L3/50273 ,  B01L3/502738 ,  B01L2200/0631 ,  B01L2200/16 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2400/0418 ,  B01L2400/0487 ,  B01L2400/0683

Abstract: A device, a system, a cartridge and a method for isolating biomolecules from biological materials are provided. The device comprises a substrate; a reagent storage location; and a self-rupturing component comprising a fluid and a pressure source embedded therein, wherein the substrate, the reagent storage location and the self-rupturing component are operationally coupled to each other. A system is provided, wherein the system comprises an extraction matrix, an enclosed matrix housing comprising a biological sample inlet, one or more biomolecule extraction reagents to extract biomolecules and at least one pressure source embedded therein, a fluidic extraction circuit; and a controller for activating the embedded pressure source. A method of isolating nucleic acids from biological materials is also provided.

Abstract translation: 提供了一种用于从生物材料中分离生物分子的装置，系统，盒和方法。 该装置包括基板; 试剂储存位置; 以及包含嵌入其中的流体和压力源的自破裂部件，其中所述基板，所述试剂储存位置和所述自破裂部件在操作上彼此耦合。 提供了一种系统，其中所述系统包括提取矩阵，包括生物样品入口的封闭矩阵壳体，用于提取生物分子的一种或多种生物分子提取试剂和嵌入其中的至少一种压力源，流体萃取回路; 以及用于启动嵌入式压力源的控制器。 还提供了从生物材料中分离核酸的方法。

公开(公告)号：US20130227644A1

公开(公告)日：2013-08-29

申请号：US13703117

申请日：2011-05-31

Applicant: Li Zhu

Inventor： Li Zhu

IPC: H04W12/06

CPC classification number: H04W12/06 ,  H04W12/08 ,  H04W84/045

Abstract: The disclosure claims a Selected Internet Protocol Traffic Offload (SIPTO) decision method and device for a Home (evolved) Node-B (H(e)NB) system, both the method and device can judge whether a H(e)NB has the SIPTO authority, and can allow the H(e)NB to implement the SIPTO if the H(e)NB has the SIPTO authority. The method and device of the disclosure can support the SIPTO of the H(e)NB.

Abstract translation: 本公开要求用于家庭（演进）节点B（H（e）NB）系统的选定的因特网协议业务卸载（SIPTO）决策方法和设备，所述方法和设备都可以判断H（e）NB是否具有 如果H（e）NB具有SIPTO权限，则可以允许H（e）NB实现SIPTO。 本公开的方法和装置可以支持H（e）NB的SIPTO。

公开(公告)号：US20130139242A1

公开(公告)日：2013-05-30

申请号：US13574470

申请日：2011-08-10

Applicant: Li Zhu

Inventor： Li Zhu

IPC: H04L29/06

CPC classification number: H04L63/0823 ,  H04L63/0869 ,  H04W12/06 ,  H04W84/045

Abstract: A method for a mutual authentication between access network devices and an access network device are disclosed by the present invention. The method includes: configuring a certificate on the access network device; performing a mutual authentication based on the certificate between the access network devices. The present invention realizes the authentication between the access network devices, thus the user data can be transmitted directly.

Abstract translation: 本发明公开了一种用于接入网络设备和接入网络设备之间的相互认证的方法。 该方法包括：在接入网络设备上配置证书; 基于接入网络设备之间的证书执行相互认证。 本发明实现了接入网络设备之间的认证，从而可以直接发送用户数据。

公开(公告)号：US07927476B2

公开(公告)日：2011-04-19

申请号：US12341233

申请日：2008-12-22

Applicant: Wei-Cheng Tian ,  Erin Jean Finehout ,  Li Zhu ,  Jun Xie

Inventor： Wei-Cheng Tian ,  Erin Jean Finehout ,  Li Zhu ,  Jun Xie

IPC: G01N27/447 ,  G01N27/453

CPC classification number: B01D57/02

Abstract: Methods and systems for injecting a sample during electrophoresis, that generally comprise: loading a sieving matrix through a first end of a separation channel; having the an end of the sieving matrix at a set distance from the intersection of the separation channel and a loading channel; pressure loading a sample through the loading channel and filling an empty portion of the separation channel; applying an electric field across the separation channel while flowing a washing buffer through the loading channel; and injecting a portion of the sample into the separation channel, wherein the portion of the sample injected is of a size that is determined by a distance between the end of the sieving matrix and the intersection of the loading and separation channels.

Abstract translation: 用于在电泳期间注射样品的方法和系统，其通常包括：通过分离通道的第一端装载筛分基质; 筛分矩阵的结束距离分离通道和加载通道的交点处一定距离; 通过加载通道加载样品并填充分离通道的空的部分; 在使洗涤缓冲液流过所述装载通道的同时，在所述分离通道上施加电场; 以及将所述样品的一部分注入所述分离通道中，其中所述注入的所述样品的所述部分具有由所述筛分矩阵的末端与所述加载和分离通道的交点之间的距离确定的尺寸。

公开(公告)号：US20090166203A1

公开(公告)日：2009-07-02

申请号：US11965794

申请日：2007-12-28

Applicant: Wei-Cheng Tian ,  Erin Jean Finehout ,  Li Zhu ,  Jun Xie ,  Shashi Thutupalli

Inventor： Wei-Cheng Tian ,  Erin Jean Finehout ,  Li Zhu ,  Jun Xie ,  Shashi Thutupalli

IPC: G01N27/26 ,  G01N27/447

CPC classification number: G01N27/44743

Abstract: A microchip for capillary electrophoresis is provided. The microchip comprises an injection channel and a separation channel configured to receive a sample through a sample well disposed on a first end of the separation channel; wherein the injection channel and the separation channel intersect to form a ‘T’ junction. The microchip further comprises a first valve disposed adjacent to the ‘T’ junction and on the separation channel and a second valve disposed at the ‘T’ junction. The second valve is a two-way valve. A sample plug is injected into an area between the ‘T’ junction and a second end of the separation channel.

Abstract translation: 提供了用于毛细管电泳的微芯片。 微芯片包括注入通道和分离通道，其被配置为通过设置在分离通道的第一端上的样品阱接收样品; 其中注入通道和分离通道相交以形成“T”结。 所述微芯片还包括邻近所述“T”结和所述分离通道设置的第一阀，以及设置在所述“T”结处的第二阀。 第二个阀是一个二通阀。 将样品塞注入到“T”结和分离通道的第二端之间的区域中。

公开(公告)号：US20090166202A1

公开(公告)日：2009-07-02

申请号：US11965791

申请日：2007-12-28

Applicant: Wei-Cheng Tian ,  Erin Jean Finehout ,  Li Zhu ,  Jun Xie ,  Shashi Thutupalli

Inventor： Wei-Cheng Tian ,  Erin Jean Finehout ,  Li Zhu ,  Jun Xie ,  Shashi Thutupalli

IPC: B01D57/02

CPC classification number: G01N27/44743 ,  G01N27/44791

Abstract: A microchip for electrophoresis is provided. The microchip comprises an injection channel and a separation channel configured to receive a sample through a sample well. The injection channel and the separation channel form a ‘T’ junction. The microchip comprises a first electrode disposed at a first end of the separation channel, a second electrode disposed in front of the ‘T’ junction and adjacent to the first electrode, a third electrode disposed at a first end of the injection channel and a fourth electrode disposed at a second end of the separation channel. A portion of the sample is injected and separated into an area between the ‘T’ junction and the fourth electrode.

Abstract translation: 提供用于电泳的微芯片。 微芯片包括注入通道和配置成通过样品阱接收样品的分离通道。 注入通道和分离通道形成“T”结。 微芯片包括设置在分离通道的第一端的第一电极，设置在“T”结的前面并与第一电极相邻的第二电极，设置在注入通道的第一端的第三电极和第四电极 电极设置在分离通道的第二端。 样品的一部分被注入并分离成“T”结和第四电极之间的区域。

公开(公告)号：US20060147449A1

公开(公告)日：2006-07-06

申请号：US11280785

申请日：2005-11-15

Applicant: Lawrence Brass ,  Li Zhu

Inventor： Lawrence Brass ,  Li Zhu

IPC: A61K39/395 ,  A61K38/46 ,  C12P21/06

CPC classification number: A61K38/1774 ,  C07K16/2803 ,  G01N33/5044 ,  G01N33/5064

Abstract: This invention relates to methods of treating platelet disorders and/or endothelial cell disorders by modulating sema4D/CD100 activity. Specifically, this invention involves the use of compounds to increase or decrease the level of soluble sema4D/CD100.

Abstract translation: 本发明涉及通过调节sema4D / CD100活性来治疗血小板障碍和/或内皮细胞疾病的方法。 具体地说，本发明涉及使用化合物来提高或降低可溶性sema4D / CD100的水平。

公开(公告)号：US20050123996A1

公开(公告)日：2005-06-09

申请号：US10947610

申请日：2004-09-21

Applicant: Li Zhu ,  Shaobing Hua ,  James Sheridan ,  Yu-Huei Lin

Inventor： Li Zhu ,  Shaobing Hua ,  James Sheridan ,  Yu-Huei Lin

IPC: C07K16/00 ,  C12N1/19 ,  C12Q1/68 ,  C40B30/04 ,  G01N33/68 ,  G01N33/53 ,  C12N1/18 ,  C12N15/74 ,  G01N33/543

CPC classification number: C40B30/04 ,  C07K16/00 ,  C07K2317/21 ,  C07K2317/55 ,  C12Q1/6897 ,  G01N33/6845 ,  C12Q2565/201

Abstract: Compositions, methods, and kits are provided for efficiently generating and screening a library of highly diverse protein complexes for their ability to bind to other proteins or oligonucleotide sequences. In one aspect of the invention, a library of expression vectors is provided for expressing the library of protein complexes. The library comprises a first nucleotide sequence encoding a first polypeptide subunit; and a second nucleotide sequence encoding a second polypeptide subunit. The first and second nucleotide sequences each independently vary within the library of expression vectors. In addition, the first and second polypeptide subunit are expressed as separate proteins which self-assemble to form a protein complex, such as a double-chain antibody fragment (dcFv or Fab) and a fully assembled antibody, in cells into which the library of expression vectors are introduced. The library of expression vectors can be efficiently generated in yeast cells through homologous recombination; and the encoded proteins complexes with high binding affinity to their target molecule can be selected by high throughput screening in vivo or in vitro.

Abstract translation: 提供了组合物，方法和试剂盒，用于有效地产生和筛选高度多样化的蛋白质复合物的文库以获得与其它蛋白质或寡核苷酸序列结合的能力。 在本发明的一个方面，提供表达载体文库用于表达蛋白质复合物的文库。 该文库包含编码第一多肽亚基的第一核苷酸序列; 和编码第二多肽亚基的第二核苷酸序列。 第一和第二核苷酸序列各自独立地在表达载体的文库内变化。 此外，第一和第二多肽亚基表达为分离的蛋白，其自组装形成蛋白复合物，例如双链抗体片段（dcFv或Fab）和完全组装的抗体，其中文库 引入表达载体。 表达载体文库可以通过同源重组在酵母细胞中有效产生; 并且可以通过体内或体外的高通量筛选来选择对其靶分子具有高结合亲和力的编码蛋白复合物。

公开(公告)号：US20050123973A1

公开(公告)日：2005-06-09

申请号：US10988485

申请日：2004-11-12

Applicant: Shaobing Hua ,  Michelle Haynes Pauling ,  Li Zhu

Inventor： Shaobing Hua ,  Michelle Haynes Pauling ,  Li Zhu

IPC: A61K39/395 ,  C07K16/00 ,  C07K16/28 ,  C12Q1/68 ,  C07K14/705 ,  G01N33/53 ,  G01N33/543

CPC classification number: C07K16/2866 ,  A61K39/39541 ,  A61K2039/505 ,  C07K16/00 ,  C07K2317/21 ,  C07K2317/622 ,  C07K2319/00 ,  A61K2300/00

Abstract: Methods are provided for selecting proteins, in particular antibodies, against a wide variety of membrane proteins. In one aspect, a method is provided for eliciting monoclonal antibody in a non-human animal administered with a target membrane protein by using a fusion protein as an immunogen that contains a peptide fragment derived from the target membrane protein. The fusion protein can also be used as a ligand for screening a library of proteins for protein-ligand binding pairs in vitro by using mRNA, ribosome, phage, or cell display. The selected proteins can be used for therapeutics and diagnostics targeting the membrane proteins.

Abstract translation: 提供了针对各种膜蛋白质选择蛋白质，特别是抗体的方法。 一方面，提供了通过使用融合蛋白作为包含衍生自靶膜蛋白的肽片段的免疫原，在施用靶膜蛋白的非人动物中引发单克隆抗体的方法。 融合蛋白也可用作通过使用mRNA，核糖体，噬菌体或细胞显示体外筛选蛋白质 - 配体结合对的蛋白质文库的配体。 所选择的蛋白质可用于靶向膜蛋白的治疗和诊断。