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    Cellular arrays comprising encoded cells
    5.
    发明申请
    Cellular arrays comprising encoded cells 审中-公开
    包含编码细胞的细胞阵列

    公开(公告)号：US20050158702A1

    公开(公告)日：2005-07-21

    申请号：US10363240

    申请日：2001-09-05

    申请人： John Stuelpnagel ,  Mark Chee

    发明人： John Stuelpnagel ,  Mark Chee

    IPC分类号： C07B61/00 ,  G01N33/50 ,  G01N33/543 ,  C12Q1/00 ,  C12M1/34 ,  G01N33/53 ,  G01N33/567

    CPC分类号： G01N33/502 ,  C40B40/00 ,  G01N33/5008 ,  G01N33/54373

    摘要： A biosensor, sensor array, sensing method and sensing apparatus are provided in which individual cells or randomly mixed populations of cells, having unique response characteristics to chemical and biological materials, are deployed in a plurality of discrete sites on a substrate. In a preferred embodiment, the discrete sites comprise microwells formed at the distal end of individual fibers within a fiber optic array. The biosensor array utilizes an optically interrogatable encoding scheme for determining the identity and location of each cell type in the array and provides for simultaneous measurements of large number of individual cell respnses to target analyses. The sensing method utilizes the unique ability of cell populations to respond to biologically significant compounds in a characteristic and detectable manner. The biosensor array and measurement method may be employed in the study of biologically active materials in situ environmental monitoring, monitoring of a variety of bioprocesses, and for high throughput screening of large combinatorial chemical libraries.

    摘要翻译： 提供了生物传感器，传感器阵列，感测方法和感测装置，其中对化学和生物材料具有独特响应特性的单个细胞或随机混合的细胞群体部署在基底上的多个离散位置。 在优选实施例中，离散位置包括在光纤阵列内的各个纤维的远端处形成的微孔。 生物传感器阵列利用光学可询问编码方案来确定阵列中每种细胞类型的身份和位置，并提供大量单个细胞反应同时测量以进行目标分析。 感测方法利用细胞群体以特征和可检测方式对生物学重要化合物的独特能力。 生物传感器阵列和测量方法可用于研究生物活性材料的原位环境监测，各种生物过程的监测以及大量组合化学文库的高通量筛选。

    Detection of nucleic acid reactions on bead arrays
    8.
    发明申请
    Detection of nucleic acid reactions on bead arrays 审中-公开
    检测珠阵列上的核酸反应

    公开(公告)号：US20050100893A1

    公开(公告)日：2005-05-12

    申请号：US10272384

    申请日：2002-10-15

    申请人： Kevin Gunderson ,  John Stuelpnagel ,  Mark Chee

    发明人： Kevin Gunderson ,  John Stuelpnagel ,  Mark Chee

    IPC分类号： C12P19/34 ,  C12Q1/68

    CPC分类号： C12Q1/6874 ,  C12Q2565/537 ,  C12Q2565/518 ,  C12Q2521/501

    摘要： The present invention is directed to methods and compositions for the use of microsphere arrays to detect and quantify a number of nucleic acid reactions. The invention finds use in genotyping, i.e. the determination of the sequence of nucleic acids, particularly alterations such as nucleotide substitutions (mismatches) and single nucleotide polymorphisms (SNPs). Similarly, the invention finds use in the detection and quantification of a nucleic acid target using a variety of amplification techniques, including both signal amplification and target amplification. The methods and compositions of the invention can be used in nucleic acid sequencing reactions as well. All applications can include the use of adapter sequences to allow for universal arrays.

    摘要翻译： 本发明涉及使用微球阵列来检测和定量许多核酸反应的方法和组合物。 本发明用于基因分型，即确定核酸序列，特别是改变，例如核苷酸取代（错配）和单核苷酸多态性（SNP）。 类似地，本发明用于使用多种扩增技术检测和定量核酸靶，包括信号放大和靶扩增两者。 本发明的方法和组合物也可用于核酸测序反应。 所有应用程序可以包括使用适配器序列来允许通用阵列。