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公开(公告)号：US20050237528A1
公开(公告)日：2005-10-27
申请号：US11086799
申请日：2005-03-22
申请人： Mark Oldham , H. Pin Kao
发明人： Mark Oldham , H. Pin Kao
IPC分类号： B01L3/00 , B01L7/00 , B01L9/00 , B01L99/00 , G01N21/25 , G01N21/27 , G01N21/64 , G01N35/00 , G01N35/02 , G01N35/04 , G01N35/10
CPC分类号： G01N35/028 , B01L3/0293 , B01L3/5025 , B01L3/5027 , B01L3/50851 , B01L3/50853 , B01L7/52 , B01L9/523 , B01L9/56 , B01L2200/021 , B01L2200/025 , B01L2200/0605 , B01L2200/0642 , B01L2200/0689 , B01L2200/142 , B01L2300/021 , B01L2300/022 , B01L2300/044 , B01L2300/046 , B01L2300/0829 , B01L2300/0851 , B01L2300/0864 , B01L2300/1883 , B01L2400/0406 , B01L2400/0409 , B01L2400/0487 , G01N21/274 , G01N21/6452 , G01N21/6486 , G01N35/0099 , G01N35/1002 , G01N35/1074 , G01N2035/00287 , G01N2035/00366 , G01N2035/0405 , G01N2035/0425 , G01N2035/1039
摘要： A heating apparatus having a support base and a microplate having a first surface and an opposing second surface. The microplate being positioned adjacent the support base. A plurality of wells are formed in the first surface of the microplate and are each sized to receive an assay therein. A transparent window is positioned adjacent the microplate opposing the support base. A transparent heating element is formed on the transparent window and is operable to heat the transparent window. A control system operably controls the transparent heating element.
摘要翻译： 一种具有支撑基座和微板的加热装置，其具有第一表面和相对的第二表面。 微板位于支撑基座附近。 在微板的第一表面中形成多个孔，并且每个孔的尺寸被设计成在其中接受测定。 透明窗口定位在与支撑基座相对的微孔板附近。 透明加热元件形成在透明窗口上并可操作以加热透明窗口。 控制系统可操作地控制透明加热元件。
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公开(公告)号：US20090176661A1
公开(公告)日：2009-07-09
申请号：US12407234
申请日：2009-03-19
申请人： Ian A. Harding , Donald Sandell , Kevin Bodner , Tim Woudenberg , Mark Oldham , H. Pin Kao , Gary Lim , John P. Bodeau , Adrian Fawcett , Robert C. Jones
发明人： Ian A. Harding , Donald Sandell , Kevin Bodner , Tim Woudenberg , Mark Oldham , H. Pin Kao , Gary Lim , John P. Bodeau , Adrian Fawcett , Robert C. Jones
IPC分类号： C40B30/10
CPC分类号： C12N15/1072 , B01L3/50851 , B01L3/50853 , B01L7/52 , B01L2200/0689 , B01L2300/021 , B01L2300/041 , B01L2300/0829 , B01L2300/12 , B01L2300/1805
摘要： A method for simultaneously determining a genetic expression profile for an individual member of a species relative to an entire standard genome for the species. The method can comprise distributing a liquid sample into an array of reaction chambers of a substrate. The array can comprise a primer set and a probe for each polynucleotide target along the entire standard genome. The liquid sample can comprise substantially all genetic material of the member. Each of the reaction chambers can comprise the primer set and the probe for at least one of the polynucleotide targets and a polymerase. The method can further comprise amplifying the liquid sample in the array, detecting a signal emitted by at least one of the probes, and identifying the genetic expression profile in response to the signal.
摘要翻译： 一种相对于物种的整个标准基因组同时测定物种的个体成员的遗传表达谱的方法。 该方法可以包括将液体样品分配到基底的反应室的阵列中。 该阵列可以包含沿着整个标准基因组的每个多核苷酸靶的引物组和探针。 液体样品可以包括构件的基本上所有的遗传物质。 每个反应室可以包含用于至少一个多核苷酸靶标和聚合酶的引物组和探针。 该方法还可以包括放大阵列中的液体样品，检测由至少一个探针发射的信号，以及响应于该信号鉴定遗传表达谱。
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公开(公告)号：US08638509B2
公开(公告)日：2014-01-28
申请号：US13252058
申请日：2011-10-03
申请人： Barry Blasenheim , H. Pin Kao , Mark Oldham
发明人： Barry Blasenheim , H. Pin Kao , Mark Oldham
IPC分类号： G02B7/02
CPC分类号： G01N21/253 , B01L3/0293 , B01L3/5025 , B01L3/5027 , B01L3/50851 , B01L3/50853 , B01L3/563 , B01L7/52 , B01L9/50 , B01L9/523 , B01L2200/021 , B01L2200/025 , B01L2200/0605 , B01L2200/0642 , B01L2200/0689 , B01L2200/142 , B01L2300/021 , B01L2300/022 , B01L2300/044 , B01L2300/046 , B01L2300/0829 , B01L2300/0851 , B01L2300/0864 , B01L2300/1883 , B01L2400/0406 , B01L2400/0409 , B01L2400/0487 , G01N21/274 , G01N21/6452 , G01N21/6486 , G01N35/0099 , G01N35/028 , G01N35/1002 , G01N35/1074 , G01N2035/00287 , G01N2035/00366 , G01N2035/0405 , G01N2035/0425 , G01N2035/1039
摘要： A camera alignment system that can enable alignment in at least one of three planes and about an axis of at least one of the planes. An alignment mount can mate to a camera and lens. The alignment mount can comprise a mechanism to adjust the camera relative to the lens to that an image plane of the camera aligns with an image plane of the lens in a predetermined orientation. One predetermined orientation can be that the image plane of the camera being parallel to the image plane of the lens.
摘要翻译： 一种相机对准系统，其能够实现三个平面中的至少一个和至少一个平面的轴线的对准。 对准安装座可与摄像机和镜头配合使用。 对准安装座可以包括一个机构，用于相机相对于镜头调整到照相机的图像平面与预定取向的透镜的图像平面对准。 一个预定方向可以是相机的图像平面平行于透镜的图像平面。
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公开(公告)号：US20100220321A1
公开(公告)日：2010-09-02
申请号：US12543459
申请日：2009-08-18
申请人： H. Pin Kao , Ian A. Harding , Achim Karger , Mark F. Oldham , Omead Ostadan , Greg Young
发明人： H. Pin Kao , Ian A. Harding , Achim Karger , Mark F. Oldham , Omead Ostadan , Greg Young
IPC分类号： G01J1/10
CPC分类号： G01N35/028 , B01L3/0293 , B01L3/5025 , B01L3/50851 , B01L3/50853 , B01L3/563 , B01L7/52 , B01L9/50 , B01L9/523 , B01L2200/021 , B01L2200/025 , B01L2200/0605 , B01L2200/0642 , B01L2200/0689 , B01L2300/022 , B01L2300/044 , B01L2300/046 , B01L2300/0829 , B01L2300/0851 , B01L2300/0864 , B01L2300/1805 , B01L2400/0406 , B01L2400/0409 , B01L2400/0487 , G01N21/274 , G01N21/6428 , G01N21/6452 , G01N2035/00366 , H04N5/361 , H04N5/367 , Y10T436/10
摘要： Methods for normalizing output from an instrument employing a reference standard or non-fluorescing substance disposed within at least one of a plurality of reaction chambers. The method comprises collecting and analyzing a signal associated with the reference standard or non-fluorescing substance to determine a normalizing bias. The normalizing bias is then applied to the data signal collected from a remainder of the plurality of reaction chambers.
摘要翻译： 用于使用设置在多个反应室中的至少一个中的参考标准或非荧光物质的仪器的输出正常化的方法。 该方法包括收集和分析与参考标准或非荧光物质相关联的信号以确定归一化偏差。 然后将归一化偏压施加到从多个反应室的其余部分收集的数据信号。
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公开(公告)号：US08040619B2
公开(公告)日：2011-10-18
申请号：US12534802
申请日：2009-08-03
申请人： Barry J. Blasenheim , H. Pin Kao , Mark F. Oldham
发明人： Barry J. Blasenheim , H. Pin Kao , Mark F. Oldham
IPC分类号： G02B7/02
CPC分类号： G01N21/253 , B01L3/0293 , B01L3/5025 , B01L3/5027 , B01L3/50851 , B01L3/50853 , B01L3/563 , B01L7/52 , B01L9/50 , B01L9/523 , B01L2200/021 , B01L2200/025 , B01L2200/0605 , B01L2200/0642 , B01L2200/0689 , B01L2200/142 , B01L2300/021 , B01L2300/022 , B01L2300/044 , B01L2300/046 , B01L2300/0829 , B01L2300/0851 , B01L2300/0864 , B01L2300/1883 , B01L2400/0406 , B01L2400/0409 , B01L2400/0487 , G01N21/274 , G01N21/6452 , G01N21/6486 , G01N35/0099 , G01N35/028 , G01N35/1002 , G01N35/1074 , G01N2035/00287 , G01N2035/00366 , G01N2035/0405 , G01N2035/0425 , G01N2035/1039
摘要： A camera alignment system that can enable alignment in at least one of three planes and about an axis of at least one of the planes. An alignment mount can mate to a camera and lens. The alignment mount can comprise a mechanism to adjust the camera relative to the lens to that an image plane of the camera aligns with an image plane of the lens in a predetermined orientation. One predetermined orientation can be that the image plane of the camera being parallel to the image plane of the lens.
摘要翻译： 一种相机对准系统，其能够实现三个平面中的至少一个和至少一个平面的轴线的对准。 对准安装座可与摄像机和镜头配合使用。 对准安装座可以包括一个机构，用于相机相对于镜头调整到照相机的图像平面与预定取向的透镜的图像平面对准。 一个预定方向可以是相机的图像平面平行于透镜的图像平面。
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公开(公告)号：US20080213755A1
公开(公告)日：2008-09-04
申请号：US10585382
申请日：2005-01-12
申请人： Timothy G. Geiser , H. Pin Kao
发明人： Timothy G. Geiser , H. Pin Kao
CPC分类号： B01L3/502715 , B01L3/502723 , B01L3/502738 , B01L7/52 , B01L2200/0684 , B01L2200/10 , B01L2300/0627 , B01L2300/0816 , B01L2300/0864 , B01L2300/1805 , B01L2400/0406 , C12Q2565/629 , Y10T436/143333
摘要： A device (420) and method for real-time amplification and detection of a large number of target nucleic acids contained in a large number of biological samples is provided. The device (420) can comprise a substrate (422) and an optically transparent cover, wherein the substrate can comprise a first surface, at least one sample receiving chamber (430), a distributor channel (434), at least one reaction chamber (428), and a vent (440) for each reaction chamber. The substrate can comprise a material having a thermal conductivity of at least about 0.25 W/m° K.
摘要翻译： 提供了一种用于实时扩增和检测包含在大量生物样品中的大量靶核酸的装置（420）和方法。 所述装置（420）可包括基底（422）和光学透明盖，其中所述基底可包括第一表面，至少一个样品接收室（430），分配器通道（434），至少一个反应室 428）和每个反应室的排气口（440）。 衬底可以包括具有至少约0.25W / m°K的热导率的材料。
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公开(公告)号：US08945481B1
公开(公告)日：2015-02-03
申请号：US12145333
申请日：2008-06-24
申请人： Mark F. Oldham , Kenneth J. Livak , Jason E. Babcoke , H. Pin Kao , Stephen J. Gunstream , Kevin S. Bodner , Douglas P. Greiner , Nigel P. Beard , Dar Bahatt
发明人： Mark F. Oldham , Kenneth J. Livak , Jason E. Babcoke , H. Pin Kao , Stephen J. Gunstream , Kevin S. Bodner , Douglas P. Greiner , Nigel P. Beard , Dar Bahatt
IPC分类号： B01L3/00
CPC分类号： B01J19/0046 , B01L3/502715 , B01L3/502738 , B01L7/52 , B01L2200/027 , B01L2200/10 , B01L2300/0654 , B01L2300/0816 , B01L2300/0819 , B01L2300/0867 , B01L2300/185 , B01L2400/0655 , G01N21/17 , G01N2201/0407
摘要： Exemplary embodiments provide microfludic devices and methods for their use. The microfluidic device can include an array of M×N reaction sites formed by intersecting a first and second plurality of fluid channels of a flow layer. The flow layer can have a matrix design and/or a blind channel design to analyze a large number of samples under a limited number of conditions. The microfluidic device can also include a control layer including a valve system for regulating solution flow through fluid channels. In addition, by aligning the control layer with the fluid channels, the detection of the microfluidic devices, e.g., optical signal collection, can be improved by piping lights to/from the reaction sites. In an exemplary embodiment, guard channels can be included in the microfluidic device for thermal cycling and/or reducing evaporation from the reaction sites.
摘要翻译： 示例性实施例提供了用于它们的微流体装置和方法。 微流体装置可以包括通过与流动层的第一和第二多个流体通道相交形成的M×N反应位点的阵列。 流层可以具有矩阵设计和/或盲通道设计，以在有限数量的条件下分析大量样品。 微流体装置还可以包括控制层，其包括用于调节通过流体通道的溶液流动的阀门系统。 此外，通过使控制层与流体通道对准，可以通过向/从反应位置的管道灯来改善微流体装置的检测，例如光学信号收集。 在示例性实施例中，保护通道可以包括在微流体装置中用于热循环和/或减少从反应位置的蒸发。
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公开(公告)号：US20120080610A1
公开(公告)日：2012-04-05
申请号：US13252058
申请日：2011-10-03
申请人： Barry J. BLASENHEIM , H. Pin Kao , Mark F. Oldham
发明人： Barry J. BLASENHEIM , H. Pin Kao , Mark F. Oldham
IPC分类号： G01N21/64
CPC分类号： G01N21/253 , B01L3/0293 , B01L3/5025 , B01L3/5027 , B01L3/50851 , B01L3/50853 , B01L3/563 , B01L7/52 , B01L9/50 , B01L9/523 , B01L2200/021 , B01L2200/025 , B01L2200/0605 , B01L2200/0642 , B01L2200/0689 , B01L2200/142 , B01L2300/021 , B01L2300/022 , B01L2300/044 , B01L2300/046 , B01L2300/0829 , B01L2300/0851 , B01L2300/0864 , B01L2300/1883 , B01L2400/0406 , B01L2400/0409 , B01L2400/0487 , G01N21/274 , G01N21/6452 , G01N21/6486 , G01N35/0099 , G01N35/028 , G01N35/1002 , G01N35/1074 , G01N2035/00287 , G01N2035/00366 , G01N2035/0405 , G01N2035/0425 , G01N2035/1039
摘要： A camera alignment system that can enable alignment in at least one of three planes and about an axis of at least one of the planes. An alignment mount can mate to a camera and lens. The alignment mount can comprise a mechanism to adjust the camera relative to the lens to that an image plane of the camera aligns with an image plane of the lens in a predetermined orientation. One predetermined orientation can be that the image plane of the camera being parallel to the image plane of the lens.
摘要翻译： 一种相机对准系统，其能够实现三个平面中的至少一个和至少一个平面的轴线的对准。 对准安装座可与摄像机和镜头配合使用。 对准安装座可以包括一个机构，用于相机相对于镜头调整到照相机的图像平面与预定取向的透镜的图像平面对准。 一个预定方向可以是相机的图像平面平行于透镜的图像平面。
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公开(公告)号：US20100193672A1
公开(公告)日：2010-08-05
申请号：US12534802
申请日：2009-08-03
申请人： Barry J. Blasenheim , H. Pin Kao , Mark F. Oldham
发明人： Barry J. Blasenheim , H. Pin Kao , Mark F. Oldham
IPC分类号： G01N21/63
CPC分类号： G01N21/253 , B01L3/0293 , B01L3/5025 , B01L3/5027 , B01L3/50851 , B01L3/50853 , B01L3/563 , B01L7/52 , B01L9/50 , B01L9/523 , B01L2200/021 , B01L2200/025 , B01L2200/0605 , B01L2200/0642 , B01L2200/0689 , B01L2200/142 , B01L2300/021 , B01L2300/022 , B01L2300/044 , B01L2300/046 , B01L2300/0829 , B01L2300/0851 , B01L2300/0864 , B01L2300/1883 , B01L2400/0406 , B01L2400/0409 , B01L2400/0487 , G01N21/274 , G01N21/6452 , G01N21/6486 , G01N35/0099 , G01N35/028 , G01N35/1002 , G01N35/1074 , G01N2035/00287 , G01N2035/00366 , G01N2035/0405 , G01N2035/0425 , G01N2035/1039
摘要： A camera alignment system that can enable alignment in at least one of three planes and about an axis of at least one of the planes. An alignment mount can mate to a camera and lens. The alignment mount can comprise a mechanism to adjust the camera relative to the lens to that an image plane of the camera aligns with an image plane of the lens in a predetermined orientation. One predetermined orientation can be that the image plane of the camera being parallel to the image plane of the lens.
摘要翻译： 一种相机对准系统，其能够实现三个平面中的至少一个和至少一个平面的轴线的对准。 对准安装座可与摄像机和镜头配合使用。 对准安装座可以包括一个机构，用于相机相对于镜头调整到照相机的图像平面与预定取向的透镜的图像平面对准。 一个预定方向可以是相机的图像平面平行于透镜的图像平面。
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公开(公告)号：US07586600B2
公开(公告)日：2009-09-08
申请号：US12176656
申请日：2008-07-21
申请人： H. Pin Kao , Ian A. Harding , Achim Karger , Mark F. Oldham , Omead Ostadan , Greg Young
发明人： H. Pin Kao , Ian A. Harding , Achim Karger , Mark F. Oldham , Omead Ostadan , Greg Young
CPC分类号： G01N35/028 , B01L3/0293 , B01L3/5025 , B01L3/50851 , B01L3/50853 , B01L3/563 , B01L7/52 , B01L9/50 , B01L9/523 , B01L2200/021 , B01L2200/025 , B01L2200/0605 , B01L2200/0642 , B01L2200/0689 , B01L2300/022 , B01L2300/044 , B01L2300/046 , B01L2300/0829 , B01L2300/0851 , B01L2300/0864 , B01L2300/1805 , B01L2400/0406 , B01L2400/0409 , B01L2400/0487 , G01N21/274 , G01N21/6428 , G01N21/6452 , G01N2035/00366 , H04N5/361 , H04N5/367 , Y10T436/10
摘要： Methods for normalizing output from an instrument employing a reference standard or non-fluorescing substance disposed within at least one of a plurality of reaction chambers. The method comprises collecting and analyzing a signal associated with the reference standard or non-fluorescing substance to determine a normalizing bias. The normalizing bias is then applied to the data signal collected from a remainder of the plurality of reaction chambers.
摘要翻译： 用于使用设置在多个反应室中的至少一个中的参考标准或非荧光物质的仪器的输出正常化的方法。 该方法包括收集和分析与参考标准或非荧光物质相关联的信号以确定归一化偏差。 然后将归一化偏压施加到从多个反应室的其余部分收集的数据信号。
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