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    Fermentative production of fine chemicals
    4.
    发明申请
    Fermentative production of fine chemicals 有权
    发酵精细化学品生产

    公开(公告)号：US20090162892A1

    公开(公告)日：2009-06-25

    申请号：US11597782

    申请日：2005-05-27

    申请人： Markus Pompejus ,  Stephan Freyer ,  Markus Lohscheidt ,  Oskar Zelder ,  Matthias Boy

    发明人： Markus Pompejus ,  Stephan Freyer ,  Markus Lohscheidt ,  Oskar Zelder ,  Matthias Boy

    IPC分类号： C12P23/00 ,  C12P1/00 ,  C12P21/00 ,  C12P19/38 ,  C12P19/30 ,  C12P19/12 ,  C12P19/04 ,  C12P13/04 ,  C12P17/18 ,  C12P17/12 ,  C12P7/64 ,  C12P7/40 ,  C12P7/48 ,  C12P7/46 ,  C12P7/42 ,  C12P7/26 ,  C12P7/02

    CPC分类号： C12P25/00 ,  C12P7/46 ,  C12P13/02 ,  C12P13/08 ,  C12P13/10 ,  C12P13/12 ,  C12P13/14 ,  C12P13/24 ,  C12P19/02 ,  C12P19/14 ,  C12P19/20 ,  Y02P20/582

    摘要： The invention relates to a process for the production of at least one microbial metabolite having at least 3 carbon atoms or at least 2 carbon atoms and at least 1 nitrogen atom by means of sugar-based microbial fermentation, comprising: a) the preparation of a sugar-containing liquid medium with a monosaccharide content of more than 20% by weight from a starch feedstock, the sugar-containing liquid medium also comprising non-starchy solid constituents of the starch feedstock; b) the fermentation of the sugar-containing liquid medium for the production of the metabolite(s); and c) depletion or isolation of at least one metabolite from the fermentation liquor, wherein a microorganism strain which produces the desired metabolite(s) is cultivated with the sugar-containing liquid medium, said liquid medium being obtained by: a1) milling the starch feedstock; and a2) liquefying the millbase in an aqueous liquid in the presence of at least one starch-liquefying enzyme, followed by saccharification using at least one saccharifying enzyme, where at least some of the millbase is liquefied by continuous or batchwise addition to the aqueous liquid.

    摘要翻译： 本发明涉及通过糖基微生物发酵生产具有至少3个碳原子或至少2个碳原子和至少1个氮原子的至少一种微生物代谢物的方法，包括：a）制备 含糖液体介质，其单糖含量大于淀粉原料的20重量％以上，所述含糖液体介质还包含淀粉原料的非淀粉状固体成分; b）用于生产代谢物的含糖液体培养基的发酵; 和c）从所述发酵液中消耗或分离至少一种代谢物，其中产生所需代谢物的微生物菌株与所述含糖液体培养基一起培养，所述液体培养基通过以下步骤获得：a1）研磨所述淀粉 原料; 和a2）在至少一种淀粉 - 液化酶的存在下在水性液体中液化所述研磨料，然后使用至少一种糖化酶进行糖化，其中至少一些所述絮凝液通过连续或分批添加至所述水性液体而液化 。

    Fermentative Production of Organic Compounds
    5.
    发明申请
    Fermentative Production of Organic Compounds 失效
    有机化合物的发酵生产

    公开(公告)号：US20080299606A1

    公开(公告)日：2008-12-04

    申请号：US12094626

    申请日：2006-11-27

    申请人： Markus Pompejus ,  Stephan Freyer ,  Markus Lohscheidt ,  Oskar Zelder ,  Matthias Boy

    发明人： Markus Pompejus ,  Stephan Freyer ,  Markus Lohscheidt ,  Oskar Zelder ,  Matthias Boy

    IPC分类号： C12P23/00 ,  C12P1/00 ,  C12P21/04 ,  C12P13/00 ,  C12P7/64 ,  C12P7/18 ,  C12P7/40 ,  C12P19/38 ,  C12P19/40 ,  C12P19/30

    CPC分类号： C12P13/08 ,  C12N1/20 ,  C12N1/22

    摘要： A process for the fermentative production of at least one organic compound having at least 3 C atoms or having at least 2 C atoms and at least one 1 N atom, comprising the following steps: a1) milling a starch feedstock, thus obtaining a millbase which comprises at least part of the nonstarchy solid constituents of the starch feedstock; a2) suspending the millbase in an aqueous liquid and hydrolysis of the starch portion in the millbase by enzymatic liquefaction and, if appropriate, subsequent saccharification, whereby a first liquid (1) which comprises mono- or oligosaccharides is obtained; and b) addition of the liquid (1) which comprises mono- or oligosaccharides together with metabolizable mono-, di- or oligosaccharides or together with a composition which comprises metabolizable mono-, di- or oligosaccharide in a concentration of at least 50% by weight and which is essentially free from solids which are insoluble in water to a fermentation medium comprising a microorganism which is capable of overproducing the organic compound under fermentation conditions.

    摘要翻译： 一种用于发酵生产至少一种具有至少3个C原子或具有至少2个C原子和至少一个1 N原子的有机化合物的方法，包括以下步骤：a1）研磨淀粉原料，从而获得一种 包括淀粉原料的至少部分非淀粉样固体成分; a2）将研磨料悬浮在含水液体中，并通过酶液化和适当的后续糖化水解淀粉部分，由此获得包含单糖或寡糖的第一液体（1）; 和b）添加包含单糖或寡糖以及可代谢的单糖，二糖或寡糖的液体（1），或与包含至少50％浓度的可代谢的单糖，二糖或寡糖的组合物一起加入 并且其基本上不含不溶于包含微生物的发酵培养基的固体，所述微生物能够在发酵条件下过量产生有机化合物。

    Fermentative production of organic compounds
    6.
    发明授权
    Fermentative production of organic compounds 失效
    发酵生产有机化合物

    公开(公告)号：US08741599B2

    公开(公告)日：2014-06-03

    申请号：US12094626

    申请日：2006-11-27

    申请人： Markus Pompejus ,  Stephan Freyer ,  Markus Lohscheidt ,  Oskar Zelder ,  Matthias Boy

    发明人： Markus Pompejus ,  Stephan Freyer ,  Markus Lohscheidt ,  Oskar Zelder ,  Matthias Boy

    IPC分类号： C12P23/00 ,  C12P19/14 ,  C12P19/02 ,  C12P13/04 ,  C12P13/08 ,  C12P13/00 ,  C12P7/66 ,  C12P1/00 ,  C12P19/38 ,  C12P7/14 ,  C12N1/00 ,  C12N1/20

    CPC分类号： C12P13/08 ,  C12N1/20 ,  C12N1/22

    摘要： A process for the fermentative production of at least one organic compound having at least 3 C atoms or having at least 2 C atoms and at least one 1 N atom, comprising the following steps: a1) milling a starch feedstock, thus obtaining a millbase which comprises at least part of the nonstarchy solid constituents of the starch feedstock; a2) suspending the millbase in an aqueous liquid and hydrolysis of the starch portion in the millbase by enzymatic liquefaction and, if appropriate, subsequent saccharification, whereby a first liquid (1) which comprises mono- or oligosaccharides is obtained; and b) addition of the liquid (1) which comprises mono- or oligosaccharides together with metabolizable mono-, di- or oligosaccharides or together with a composition which comprises metabolizable mono-, di- or oligosaccharide in a concentration of at least 50% by weight and which is essentially free from solids which are insoluble in water to a fermentation medium comprising a microorganism which is capable of overproducing the organic compound under fermentation conditions.

    摘要翻译： 一种用于发酵生产至少一种具有至少3个C原子或具有至少2个C原子和至少一个1 N原子的有机化合物的方法，包括以下步骤：a1）研磨淀粉原料，从而获得一种 包括淀粉原料的至少部分非淀粉样固体成分; a2）将研磨料悬浮在含水液体中，并通过酶液化和适当的后续糖化水解淀粉部分，由此获得包含单糖或寡糖的第一液体（1）; 和b）添加包含单糖或寡糖以及可代谢的单糖，二糖或寡糖的液体（1），或与包含至少50％浓度的可代谢的单糖，二糖或寡糖的组合物一起加入 并且其基本上不含不溶于包含微生物的发酵培养基的固体，所述微生物能够在发酵条件下过量产生有机化合物。

    Method for the production of glutaconate
    9.
    发明授权
    Method for the production of glutaconate 有权
    生产戊二酸的方法

    公开(公告)号：US08778645B2

    公开(公告)日：2014-07-15

    申请号：US13144616

    申请日：2010-01-15

    申请人： Oskar Zelder ,  Wolfgang Buckel ,  Ivana Djurdjevic

    发明人： Oskar Zelder ,  Wolfgang Buckel ,  Ivana Djurdjevic

    IPC分类号： C12P21/06 ,  C12P7/44 ,  C12N9/00 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04

    CPC分类号： C12P7/44 ,  C12N9/0006 ,  C12N9/13 ,  C12N9/88 ,  C12P7/50

    摘要： The present invention relates to a novel method for the biocatalytic production of unsaturated dicarboxylic acids by cultivating a recombinant microorganism co-expressing a glutaconate CoA-transferase and a 2-hydroxyglutaryl-CoA dehydratase system. The present invention also relates to corresponding recombinant hosts, recombinant vectors, expression cassettes and nucleic acids suitable for preparing such hosts as well as a method of preparing polyamide or polyester copolymers making use of said dicarboxylic acids as obtained by said biocatalytic production method.

    摘要翻译： 本发明涉及通过培养共表达戊二酸CoA转移酶和2-羟基戊二酰辅酶A脱水酶系统的重组微生物来生物催化生产不饱和二羧酸的新方法。 本发明还涉及适用于制备这种宿主的相应的重组宿主，重组载体，表达盒和核酸以及通过所述生物催化生产方法获得的制备使用所述二羧酸的聚酰胺或聚酯共聚物的方法。

    Method for producing methionine using a microorganism with reduced expression activity of a negative regulator of methionine biosynthesis
    10.
    发明授权
    Method for producing methionine using a microorganism with reduced expression activity of a negative regulator of methionine biosynthesis 有权
    使用具有降低甲硫氨酸生物合成负调节子的表达活性的微生物生产甲硫氨酸的方法

    公开(公告)号：US08389251B2

    公开(公告)日：2013-03-05

    申请号：US13018119

    申请日：2011-01-31

    申请人： Markus Pompejus ,  Burkhard Kröger ,  Hartwig Schröder ,  Oskar Zelder ,  Gregor Haberhauer ,  Stefan Haefner ,  Corrinna Klopprogge

    发明人： Markus Pompejus ,  Burkhard Kröger ,  Hartwig Schröder ,  Oskar Zelder ,  Gregor Haberhauer ,  Stefan Haefner ,  Corrinna Klopprogge

    IPC分类号： C12P13/12 ,  C12N15/74

    CPC分类号： C07K14/34

    摘要： Isolated nucleic acid molecules, designated MR nucleic acid molecules, which encode novel MR proteins from Corynebacterium glutamicum are described which are involved in biosynthesis of a fine chemical, e.g., methionine biosynthesis. The invention also provides antisense nucleic acid molecules, recombinant expression vectors containing MR nucleic acid molecules, and host cells into which the expression vectors have been introduced. The invention still further provides methods of producing methionine from microorganisms, e.g., C. glutamicum, which involve culturing recombinant microorganisms which overexpress or underexpress at least one MR molecule of the invention under conditions such that methionine is produced. Also featured are methods of producing a fine chemical, e.g., methionine, which involve culturing recombinant microorganisms having selected MR genes deleted or mutated under conditions such that the fine chemical, e.g., methionine, is produced.

    摘要翻译： 描述了编码来自谷氨酸棒杆菌的新型MR蛋白质的分离的核酸分子，称为MR核酸分子，其涉及精细化学品的生物合成，例如甲硫氨酸生物合成。 本发明还提供反义核酸分子，含有MR核酸分子的重组表达载体和已经引入表达载体的宿主细胞。 本发明还提供了从微生物例如谷氨酸棒杆菌制备甲硫氨酸的方法，其涉及在产生甲硫氨酸的条件下培养过表达或低表达本发明的至少一种MR分子的重组微生物。 还特征在于生产精细化学品的方法，例如甲硫氨酸，其涉及在产生精细化学物质例如甲硫氨酸的条件下培养具有选择的MR基因缺失或突变的重组微生物。