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    DNA MICRO-ARRAY HAVING STANDARD PROBE AND KIT INCLUDING THE ARRAY
    1.
    发明申请
    DNA MICRO-ARRAY HAVING STANDARD PROBE AND KIT INCLUDING THE ARRAY 审中-公开
    具有标准探针和包装阵列的DNA微阵列

    公开(公告)号：US20090093373A1

    公开(公告)日：2009-04-09

    申请号：US12264241

    申请日：2008-11-03

    申请人： Masahiro Kawaguchi ,  Tadashi Okamoto ,  Hiromitsu Takase ,  Hiroyuki Hashimoto

    发明人： Masahiro Kawaguchi ,  Tadashi Okamoto ,  Hiromitsu Takase ,  Hiroyuki Hashimoto

    IPC分类号： C40B30/04 ,  C40B30/10

    CPC分类号： C12Q1/6837

    摘要： To provide a DNA micro-array having a nucleic acid probe immobilized on a substrate, which is used to detect a molecule of a target nucleic acid contained in a sample and has a substantially complementary base sequence to the target base sequence of the nucleic acid molecule, including at least one probe selected from the group consisting of: at least one internal standard probe for assay of PCR of the target nucleic acid; at least one external standard probe for a detection operation and assay of an amount of the probe; and a probe for measurement of the amount or density of the nucleic acid probe, formed by the same method as the nucleic acid probe.

    摘要翻译： 提供具有固定在底物上的核酸探针的DNA微阵列，其用于检测样品中所含的靶核酸的分子，并且与核酸分子的靶碱基序列具有基本上互补的碱基序列 包括至少一种选自以下的探针：用于测定靶核酸的PCR的至少一个内标物探针; 用于检测操作的至少一个外部标准探针和探针的量的测定; 以及通过与核酸探针相同的方法形成的用于测量核酸探针的量或密度的探针。

    Method for acquiring information of a biochip using time of flight secondary ion mass spectrometry and an apparatus for acquiring information for the application thereof
    2.
    发明授权
    Method for acquiring information of a biochip using time of flight secondary ion mass spectrometry and an apparatus for acquiring information for the application thereof 有权
    使用飞行时间二次离子质谱法获取生物芯片的信息的方法和用于获取其应用信息的装置

    公开(公告)号：US07188031B1

    公开(公告)日：2007-03-06

    申请号：US10601777

    申请日：2003-06-24

    申请人： Tadashi Okamoto ,  Hiromitsu Takase ,  Hiroyuki Hashimoto

    发明人： Tadashi Okamoto ,  Hiromitsu Takase ,  Hiroyuki Hashimoto

    IPC分类号： G01N33/48 ,  G01R23/02 ,  C12Q1/00 ,  C12M1/34

    CPC分类号： G01N23/2258 ,  H01J49/16 ,  H01J49/40

    摘要： A measurement method is provided, which enables to obtain a two-dimensional image with better quantitative-ability by suppressing the influence of the charge-up, when the two-dimensional secondary ion image is obtained for a biological material fixed on a substrate having a high resistivity by utilizing a TOF-SIMS method in a certain wide area. A two-dimensional image having considerably high positioning resolution-ability can be obtained by the procedure in which the pulsed primary ion beam is irradiated at a spot, and the pulse-wise spot-applications of the primary ion beam and the simultaneous detection of the secondary ion generated from the irradiated primary ion beam proceed along with a discontinuous scanning pattern, and eventually the results of these secondary ion measurements are reconstructed into a two-dimensional image in line with the aforementioned discontinuous scanning pattern.

    摘要翻译： 提供了一种测量方法，其能够通过抑制电荷的影响来获得具有更好的定量能力的二维图像，当获得固定在具有 在某一广泛领域利用TOF-SIMS方法具有高电阻率。 具有相当高的定位分辨能力的二维图像可以通过脉冲一次离子束在一个点照射的过程和一次离子束的脉冲点应用和同时检测 从照射的一次离子束产生的二次离子与不连续扫描图案一起进行，最终将这些二次离子测量的结果重建为与上述不连续扫描图案一致的二维图像。

    METHOD FOR ACQUIRING INFORMATION OF A BIOCHIP USING TIME OF FLIGHT SECONDARY ION MASS SPECTROMETRY AND AN APPARATUS FOR ACQUIRING INFORMATION FOR THE APPLICATION THEREOF
    3.
    发明申请
    METHOD FOR ACQUIRING INFORMATION OF A BIOCHIP USING TIME OF FLIGHT SECONDARY ION MASS SPECTROMETRY AND AN APPARATUS FOR ACQUIRING INFORMATION FOR THE APPLICATION THEREOF 有权
    使用飞行次级离子质谱的时间获取生物信息的方法和获取其应用信息的装置

    公开(公告)号：US20070042496A1

    公开(公告)日：2007-02-22

    申请号：US10601777

    申请日：2003-06-24

    申请人： Tadashi Okamoto ,  Hiromitsu Takase ,  Hiroyuki Hashimoto

    发明人： Tadashi Okamoto ,  Hiromitsu Takase ,  Hiroyuki Hashimoto

    IPC分类号： G06F19/00 ,  G01N33/50

    CPC分类号： G01N23/2258 ,  H01J49/16 ,  H01J49/40

    摘要： A measurement method is provided, which enables to obtain a two-dimensional image with better quantitative-ability by suppressing the influence of the charge-up, when the two-dimensional secondary ion image is obtained for a biological material fixed on a substrate having a high resistivity by utilizing a TOF-SIMS method in a certain wide area. A two-dimensional image having considerably high positioning resolution-ability can be obtained by the procedure in which the pulsed primary ion beam is irradiated at a spot, and the pulse-wise spot-applications of the primary ion beam and the simultaneous detection of the secondary ion generated from the irradiated primary ion beam proceed along with a discontinuous scanning pattern, and eventually the results of these secondary ion measurements are reconstructed into a two-dimensional image in line with the aforementioned discontinuous scanning pattern.

    摘要翻译： 提供了一种测量方法，其能够通过抑制电荷的影响来获得具有更好的定量能力的二维图像，当获得固定在具有 在某一广泛领域利用TOF-SIMS方法具有高电阻率。 具有相当高的定位分辨能力的二维图像可以通过脉冲一次离子束在一个点照射的过程和一次离子束的脉冲点应用和同时检测 从照射的一次离子束产生的二次离子与不连续扫描图案一起进行，最终将这些二次离子测量的结果重建为与上述不连续扫描图案一致的二维图像。

    Method for detecting bioindividuals by use of nonnatural type nucleic acid probe
    7.
    发明授权
    Method for detecting bioindividuals by use of nonnatural type nucleic acid probe 失效
    通过使用非天然型核酸探针检测生物个体的方法

    公开(公告)号：US5545521A

    公开(公告)日：1996-08-13

    申请号：US163725

    申请日：1993-12-09

    申请人： Tadashi Okamoto ,  Yoshinori Tomida ,  Nobuko Yamamoto ,  Masahiro Kawaguchi

    发明人： Tadashi Okamoto ,  Yoshinori Tomida ,  Nobuko Yamamoto ,  Masahiro Kawaguchi

    IPC分类号： C12N15/09 ,  C12Q1/68 ,  C12Q1/70 ,  C07H21/04 ,  C12N15/00

    CPC分类号： C12Q1/6888 ,  C12Q1/68 ,  C12Q1/6813 ,  Y10S436/80 ,  Y10T436/143333

    摘要： A method for detecting live individuals comprises a step of reacting a sample with a probe having a nonnatural type nucleic acid and a label substance linked with the nucleic acid, which nonnatural type nucleic acid contains a base sequence complementary to a base sequence of a target nucleic acid in the live target individuals to be detected and which probe is able to be incorporated into the live target individuals; and detecting the probe incorporated into the live target individuals by the utilization of the label substance of the probe when the live target individuals are present in the sample. A probe has a nonnatural type nucleic acid and a label substance linked with the nucleic acid, which nonatural type nucleic acid contains a base sequence complementary to a base sequence of a target nucleic acid in a live target individuals to be detected, and the probe is able to be incorporated into the live target individuals.

    摘要翻译： 用于检测活体个体的方法包括使样品与具有非天然型核酸的探针和与核酸连接的标记物质反应的步骤，该非天然型核酸含有与靶核酸的碱基序列互补的碱基序列 活体目标中的酸被检测，哪个探针能够被并入活的目标个体中; 并且当活体目标个体存在于样品中时，通过利用探针的标记物质来检测结合到活的目标个体中的探针。 探针具有非天然型核酸和与核酸连接的标记物质，该非天然型核酸含有与要检测的活体目标个体中靶核酸的碱基序列互补的碱基序列，探针为 能够被纳入现场目标的个人。

    Pharmaceutical compositions containing pyrylium compounds, pyrylium salts and process for manufacturing a medicament containing the aforesaid compounds
    8.
    发明授权
    Pharmaceutical compositions containing pyrylium compounds, pyrylium salts and process for manufacturing a medicament containing the aforesaid compounds 失效
    含有吡喃鎓化合物，吡喃鎓盐的药物组合物和制备含有上述化合物的药物的方法

    公开(公告)号：US6124342A

    公开(公告)日：2000-09-26

    申请号：US878125

    申请日：1997-06-18

    申请人： Tadashi Okamoto ,  Nobuko Yamamoto ,  Masahiro Kawaguchi

    发明人： Tadashi Okamoto ,  Nobuko Yamamoto ,  Masahiro Kawaguchi

    IPC分类号： C07D335/02 ,  A61K31/33 ,  A61K31/35 ,  A61K31/351 ,  A61K31/38 ,  A61K31/382 ,  A61K41/00 ,  A61P35/00 ,  A61P43/00 ,  C07D309/34 ,  C07D345/00

    CPC分类号： A61K31/382 ,  A61K31/33 ,  A61K31/35 ,  A61K31/38 ,  A61K41/0057

    摘要： The present invention provide pharmaceutical compositions containing a pyrylium compound, a thiopyrylium compound, a selenopyrylium compound or a telluropyrylium compound or a salt of any of the aforesaid compounds as active ingredient. The compounds are selectively absorbed by cancer cells or similar growths in the human or animal body and can be used to bring about distruction of the unwanted growth on irradiation with light of wavelength 600 nm to 1000 nm. The invention also provides a method for the treatment of the human or animal body which comprises administering the compound to a human or animal and irradiating a locus in said animal where the compound is absorbed in order to kill cells at that locus. It further comprises the use of the aforesaid compound for the making of a medicament for use in the treatment of cancer in humans and animals.

    摘要翻译： 本发明提供药物组合物，其含有吡喃鎓化合物，硫代吡喃鎓化合物，硒代吡喃鎓化合物或替硝基吡喃鎓化合物或任何上述化合物的盐作为活性成分。 这些化合物被人类或动物体中的癌细胞或类似的生长选择性地吸收，并且可以用于在600nm至1000nm的波长的光照射下引起不想要的生长的破坏。 本发明还提供了一种治疗人或动物体的方法，其包括将化合物给予人或动物，并照射所述动物中吸收化合物的位点，以杀死该位点处的细胞。 它还包括使用上述化合物制备用于治疗人和动物中的癌症的药物。

    Fluorescent stain containing pyrylium salt and fluorescent staining method of biological sample
    9.
    发明授权
    Fluorescent stain containing pyrylium salt and fluorescent staining method of biological sample 失效
    荧光染料含有吡喃鎓盐和荧光染色法的生物样品

    公开(公告)号：US6022961A

    公开(公告)日：2000-02-08

    申请号：US782798

    申请日：1997-01-13

    申请人： Nobuko Yamamoto ,  Tadashi Okamoto ,  Yoshinori Tomida ,  Takeshi Miyazaki ,  Masahiro Kawaguchi

    发明人： Nobuko Yamamoto ,  Tadashi Okamoto ,  Yoshinori Tomida ,  Takeshi Miyazaki ,  Masahiro Kawaguchi

    IPC分类号： G01N33/48 ,  C07D309/34 ,  C07D335/02 ,  C07D345/00 ,  C09B57/00 ,  C09K11/06 ,  C12Q1/68 ,  G01N1/30 ,  G01N33/50 ,  G01N33/52 ,  C07H21/04 ,  C07D335/00

    CPC分类号： G01N33/52 ,  C09B57/00 ,  C12Q1/6816 ,  G01N1/30 ,  C12Q2304/10 ,  C12Q2563/173

    摘要： A stain for a nucleic acid characterized by containing, as an effective component, a pyrylium salt compound having Y as a negative portion and a pyrylium ring or a pyrylium-similar ring, wherein the ring is comprised of X selected from O, S, Se and Te as a hetero atom and has substituents R.sup.1, R.sup.2 and R.sup.3, wherein each of R.sup.1 and R.sup.2 is independently a hydrogen atom, sulfonate group, amino group, substituted or unsubstituted aryl group, etc., R.sup.3 is -A or -L-A, L is -L.sup.1 -, -L.sup.2 -L.sup.3 - or -L.sup.4 -L.sup.5 -L.sup.6 -, and each of L.sup.1 to L.sup.6 is independently --(CH.dbd.CH)--, a divalent group derived from the substituted or unsubstituted aryl group, etc., A is a substituted or unsubstituted aryl group, etc., and Y.sup.- is an anion. A method for detecting a nucleic acid which comprises the steps of reacting a sample with the stain, and then detecting, by an optical means, the double-strand nucleic acid stained with the stain in the case that the double-strand nucleic acid is contained in the sample.

    摘要翻译： 一种核酸的污渍，其特征在于含有Y为负极的吡喃鎓盐化合物作为有效成分，以及吡喃鎓环或吡啶类似的环，其中该环由选自O，S，Se 和Te作为杂原子，并且具有取代基R 1，R 2和R 3，其中R 1和R 2各自独立地为氢原子，磺酸酯基，氨基，取代或未取代的芳基等，R 3为-A或-LA， L为-L1-，-L2-L3-或-L4-L5-L6-，L1至L6各自独立地为 - （CH = CH） - ，衍生自取代或未取代的芳基的二价基团等 A是取代或未取代的芳基等，Y-是阴离子。 一种检测核酸的方法，其包括以下步骤：使样品与染色剂反应，然后通过光学方法检测在含有双链核酸的情况下用染色剂染色的双链核酸 在样品中。