公开(公告)号：US20050144662A1

公开(公告)日：2005-06-30

申请号：US10942954

申请日：2004-09-17

申请人： Masataka Nakamura ,  Kazuo Sugamura ,  Shoichi Takano ,  Kinya Nagata ,  Hiroyuki Hirai

发明人： Masataka Nakamura ,  Kazuo Sugamura ,  Shoichi Takano ,  Kinya Nagata ,  Hiroyuki Hirai

IPC分类号： A01K67/027 ,  C07K14/705 ,  C12N15/09 ,  C12N15/85 ,  G01N33/15 ,  G01N33/50

CPC分类号： C12N15/8509 ,  A01K67/0276 ,  A01K2217/075 ,  A01K2227/105 ,  A01K2267/03 ,  C07K14/705

摘要： A knockout mouse whose genome includes an inactivated CRTH2 gene. The knockout mouse is obtained by subjecting to a serial passage a chimeric mouse originating from an early embryo to which a CRTH2-gene-knocked-out mouse embryonic stem cell has been introduced. Also disclosed is a detection method which includes employing, as an index, pathological condition of the knockout mouse to which the test substance has been administered, to thereby detect, in vivo, characteristics of a test substance in relation to CRTH2, or functions of CRTH2 in the living body.

摘要翻译： 基因组的敲除小鼠，其包含失活的CRTH2基因。 敲除小鼠通过连续通过源自已经引入了CRTH2基因敲除的小鼠胚胎干细胞的早期胚胎的嵌合小鼠获得。 还公开了一种检测方法，其包括使用已经施用受检物质的敲除小鼠的病理状态作为指标，从而检测体内与CRTH2相关的测试物质的特征，或CRTH2的功能 在活体内。

公开(公告)号：US20100015643A1

公开(公告)日：2010-01-21

申请号：US12301535

申请日：2007-05-21

申请人： Chuwa Tei ,  Kouji Orihara ,  Kinya Nagata ,  Ryutaro Oba ,  Hiroyuki Hirai

发明人： Chuwa Tei ,  Kouji Orihara ,  Kinya Nagata ,  Ryutaro Oba ,  Hiroyuki Hirai

IPC分类号： G01N33/567

CPC分类号： G01N33/5091 ,  G01N33/5094 ,  G01N33/6893 ,  G01N2800/26

摘要： There is provided a method of quantitative determination using a flow cytometer, with which quantitative determination of cell surface protein can be effected more accurately than with current methods. The inventors have achieved the present invention by providing a method of quantitative determination of sites, per cell of a test sample, at which an antibody is bound to an antigen protein (sites/cell), characterized by preparing a calibration curve on the basis of fluorescent intensities obtained through measuring with a flow cytometer the amount of labeled antibodies against antigen protein which are bound to two or more groups of beads carrying known and different amounts of the antigen protein, and numeric values of the known amounts of the antigen protein, and further by measuring, with the flow cytometer, labeled antibodies against antigen protein after they have been reacted with test cells derived from a blood sample of a test subject, whereby digitalization is effected through comparison and conversion between the calibration curve and a fluorescence intensity obtained.

摘要翻译： 提供了一种使用流式细胞仪进行定量测定的方法，可以比目前的方法更准确地进行细胞表面蛋白的定量测定。 发明人通过提供定量测定抗体与抗原蛋白（位点/细胞）结合的测试样品的每个细胞的位点的方法来实现本发明，其特征在于基于以下方式制备校准曲线 通过用流式细胞仪测量获得的荧光强度，结合到携带已知和不同量的抗原蛋白的两个或更多个珠粒组的抗原蛋白的标记抗体的量，以及已知量的抗原蛋白的数值，以及 进一步通过用流式细胞仪测量抗原蛋白的抗体，在与测试受试者的血液样本的测试细胞反应之后，通过比较和转换校正曲线和获得的荧光强度来进行数字化。

公开(公告)号：US06884593B1

公开(公告)日：2005-04-26

申请号：US10069148

申请日：2000-08-22

申请人： Hiroyuki Hirai ,  Kazuyuki Ogawa ,  Kinya Nagata ,  Syoichi Takano

发明人： Hiroyuki Hirai ,  Kazuyuki Ogawa ,  Kinya Nagata ,  Syoichi Takano

IPC分类号： G01N33/88 ,  G01N33/53 ,  G01N33/567

CPC分类号： G01N33/88 ,  G01N2500/04

摘要： An object of the present invention is to provide a method of identifying a substance which acts on a newly found second human prostaglandin D2 receptor subtype that differs from the DP receptor, and is useful for treating or preventing various diseases. In order to attain the object, the present invention provides a method of identifying properties of a test substance with respect to a human prostaglandin D receptor, by establishing correlation between the effect of a test substance on human CRTH2 with the effect of the test substance on the human prostaglandin D receptor.

摘要翻译： 本发明的目的是提供一种鉴定作用于新发现的与DP受体不同的第二人前列腺素D 2受体亚型的物质的方法，并且可用于治疗或预防各种 疾病 为了达到上述目的，本发明提供了一种鉴定被测物质相对于人前列腺素D受体的性质的方法，其特征在于，测试物质对人CRTH2的作用与受检物质对 人类前列腺素D受体。

公开(公告)号：US09580689B2

公开(公告)日：2017-02-28

申请号：US13811572

申请日：2011-07-22

申请人： Nobuaki Kikyo ,  Hiroyuki Hirai

发明人： Nobuaki Kikyo ,  Hiroyuki Hirai

IPC分类号： C12N5/00 ,  C12N5/074 ,  C07K14/47 ,  C12N15/85

CPC分类号： C12N5/0696 ,  A01K2207/12 ,  A01K2227/105 ,  C07K14/4702 ,  C07K2319/71 ,  C12N15/8509 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/606 ,  C12N2506/1307 ,  C12N2510/00 ,  C12N2740/15041

摘要： Described herein is a major breakthrough in nuclear reprogramming and induced pluripotent stem cell (iPSC) technology. Fusion of the powerful transcription activation domain (TAD) of MyoD to the Oct4 protein makes iPSCs generation faster, more efficient, purer, safer and feeder-free. Also, disclosed herein is the first report of the use of a TAD fused to a transcription factor as a method for making iPSCs. By combining transcription factors and TADs, this approach to nuclear reprogramming can have a range of applications from inducing pluriopotency to inducing transdifferentiation without transitioning through iPSCs.

摘要翻译： 这里描述的是核重新编程和诱导多能干细胞（iPSC）技术的重大突破。 将MyoD强大的转录激活结构域（TAD）融合到Oct4蛋白质中，使得iPSC生成更快，更有效，更纯净，更安全，更无饲料。 此外，本文公开的是使用与转录因子融合的TAD作为制备iPSC的方法的第一个报告。 通过组合转录因子和TAD，这种核重新编程方法可以具有诱导多能性诱导转分化而不通过iPSC过渡的应用范围。

公开(公告)号：US20130195812A1

公开(公告)日：2013-08-01

申请号：US13811572

申请日：2011-07-22

申请人： Nobuaki Kikyo ,  Hiroyuki Hirai

发明人： Nobuaki Kikyo ,  Hiroyuki Hirai

IPC分类号： C12N5/071

CPC分类号： C12N5/0696 ,  A01K2207/12 ,  A01K2227/105 ,  C07K14/4702 ,  C07K2319/71 ,  C12N15/8509 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/606 ,  C12N2506/1307 ,  C12N2510/00 ,  C12N2740/15041

摘要： Described herein is a major breakthrough in nuclear reprogramming and induced pluripotent stem cell (iPSC) technology. Fusion of the powerful transcription activation domain (TAD) of MyoD to the Oct4 protein makes iPSCs generation faster, more efficient, purer, safer and feeder-free. Also, disclosed herein is the first report of the use of a TAD fused to a transcription factor as a method for making iPSCs. By combining transcription factors and TADs, this approach to nuclear reprogramming can have a range of applications from inducing pluriopotency to inducing transdifferentiation without transitioning through iPSCs.

摘要翻译： 这里描述的是核重新编程和诱导多能干细胞（iPSC）技术的重大突破。 将MyoD强大的转录激活结构域（TAD）融合到Oct4蛋白质中，使得iPSC生成更快，更有效，更纯净，更安全，更无饲料。 此外，本文公开的是使用与转录因子融合的TAD作为制备iPSC的方法的第一个报告。 通过组合转录因子和TAD，这种核重新编程方法可以具有诱导多能性诱导转分化而不通过iPSC过渡的应用范围。

公开(公告)号：US20090130022A1

公开(公告)日：2009-05-21

申请号：US11916391

申请日：2006-06-06

申请人： Junji Nishigaki ,  Hiroyuki Hirai ,  Masayoshi Kojima ,  Kazuhiro Aikawa

发明人： Junji Nishigaki ,  Hiroyuki Hirai ,  Masayoshi Kojima ,  Kazuhiro Aikawa

IPC分类号： A61B5/055 ,  A61K9/127

CPC分类号： A61K49/1812

摘要： A liposome containing a phosphatidylcholine and a phosphatidylserine in combination as membrane components at a phosphatidylcholine:phosphatidylserine molar ratio of 3:1 to 1:2, and said liposome containing superparamagnetic particles having a mean particle size not less than 1 nm and not more than 50 nm. A means for selectively accumulating an MRI contrast medium in a lesion of a vascular disease caused by abnormal proliferation of vascular smooth muscle cells such as arteriosclerosis and restenosis after PTCA is provided.

摘要翻译： 含有磷脂酰胆碱和磷脂酰丝氨酸的磷脂酰丝氨酸组合为磷脂酰胆碱的膜组分：磷脂酰丝氨酸摩尔比为3:1至1:2，所述脂质体含有平均粒度不小于1nm且不大于50的超顺磁性颗粒 nm。 用于选择性地累积由血管平滑肌细胞的异常增殖引起的血管疾病的病变中的MRI造影剂的手段，所述血管病变由PTCA提供之后的动脉硬化和再狭窄引起。

公开(公告)号：US07462300B2

公开(公告)日：2008-12-09

申请号：US10982876

申请日：2004-11-08

申请人： Hiroyuki Hirai ,  Keiko Sugihara ,  Junji Nishigaki

发明人： Hiroyuki Hirai ,  Keiko Sugihara ,  Junji Nishigaki

IPC分类号： C09K11/56 ,  B82B1/00 ,  C01G9/08

CPC分类号： C09K11/574 ,  B82Y15/00 ,  C09K11/584 ,  G01N33/582 ,  G01N33/588 ,  Y10S977/773 ,  Y10S977/786 ,  Y10S977/813 ,  Y10S977/824 ,  Y10S977/834 ,  Y10T428/2991

摘要： A doped-type metal sulfide phosphor nanoparticle, whose surface is modified with a surface modifier, the surface modifier being a compound of formula [I]: HS-L-W  Formula [I] wherein L is a divalent linking group; and W is COOM or NH2, in which M is a hydrogen atom, an alkali metal atom, or NX4, in which X is a hydrogen atom or an alkyl group; a dispersion containing the nanoparticle; and a method of producing the nanoparticle or the dispersion.

摘要翻译： 掺杂型金属硫化物荧光体纳米颗粒，其表面用表面改性剂改性，表面改性剂为式[I]的化合物：<？in-line-formula description =“In-line Formulas”end =“lead” α> HS-LW式[I] <？in-line-formula description =“In-line formula”end =“tail”？>其中L是二价连接基团; W为COOM或NH 2，其中M为氢原子，碱金属原子或NX4，其中X为氢原子或烷基; 含有纳米颗粒的分散体; 以及制备纳米颗粒或分散体的方法。

公开(公告)号：US20080156736A1

公开(公告)日：2008-07-03

申请号：US11883742

申请日：2006-02-08

申请人： Hiroyuki Hirai ,  Masayoshi Kojima

发明人： Hiroyuki Hirai ,  Masayoshi Kojima

IPC分类号： B03C1/02 ,  H01F1/42

CPC分类号： B03C1/01 ,  B82Y25/00 ,  H01F1/0054

摘要： A magnetic composite body comprising magnetic nanoparticles having a number average particle diameter of 1 to 50 nm and having a compound represented by formula (I) fixed on their surfaces, and a method comprising bringing the magnetic composite body into contact with a test piece followed by magnetic separation: R1O—(CH(R2)CH2O)n-L-X   Formula (I) wherein R1 represents a hydrogen atom, an alkyl or alkenyl group having a carbon chain length of 1 to 20, or a substituted or unsubstituted, aryl or heterocyclic group; R2 represents a hydrogen atom or a methyl group; L may be present or not present, and, when L is present, L represents an alkylene or alkenylene group having a carbon chain length of 1 to 10 which may have a branched chain or a substituent; X represents a hydrogen atom, a carboxylic acid group, a phosphoric acid group, or a sulfonic acid group; and n represents an integer of 1 to 10

摘要翻译： 一种磁性复合体，其包含数均粒径为1〜50nm的磁性纳米颗粒，并且具有固定在其表面上的式（I）表示的化合物，以及包括使磁性复合体与试验片接触的方法， 磁分离：<？in-line-formula description =“In-line Formulas”end =“lead”？> R O-（CH（R 2）2 CH 式（I）<？in-line-formula description =“In-line formula”end =“tail”？>其中R ^{1 表示氢原子，碳链长度为1-20的烷基或烯基，或取代或未取代的芳基或杂环基; R 2表示氢原子或甲基; L可以存在或不存在，并且当存在L时，L表示碳链长度为1至10的亚烷基或亚烯基，其可以具有支链或取代基; X表示氢原子，羧酸基，磷酸基或磺酸基; n表示1〜10的整数}

公开(公告)号：US07242591B2

公开(公告)日：2007-07-10

申请号：US10530518

申请日：2003-10-06

申请人： Tatsuro Imamura ,  Yuji Yamaguchi ,  Kazuhiro Shinozaki ,  Satoshi Shibazaki ,  Yoshitaka Fukuoka ,  Hiroyuki Hirai ,  Osamu Shimada ,  Kenji Sasaoka ,  Kenichi Matsumura

发明人： Tatsuro Imamura ,  Yuji Yamaguchi ,  Kazuhiro Shinozaki ,  Satoshi Shibazaki ,  Yoshitaka Fukuoka ,  Hiroyuki Hirai ,  Osamu Shimada ,  Kenji Sasaoka ,  Kenichi Matsumura

IPC分类号： H05K1/18

CPC分类号： H05K1/184 ,  H05K1/023 ,  H05K1/186 ,  H05K3/3442 ,  H05K3/4602 ,  H05K2201/10636 ,  Y02P70/611 ,  Y10T29/49126 ,  Y10T29/4913 ,  Y10T29/49146 ,  Y10T29/49147 ,  Y10T29/49155 ,  Y10T29/49165 ,  Y10T29/49789 ,  Y10T29/49798

摘要： To provide a component built-in wiring board and a manufacturing method thereof capable of further improving component mounting density without deteriorating reliability. The component built-in wiring board includes: a conductive layer (34, 35) extending in a thickness direction of the board and buried in the board without being exposed from an upper and a lower surface of the board; an electrical/electronic component (33) having a terminal and buried in the board with the terminal facing the buried conductive layer; a connecting member (36, 37) provided in a gap between the terminal of the buried electrical/electronic component and the conductive layer to electrically/mechanically connect the terminal and the conductive layer; and two upper and lower insulating layers (11, 15) which cover an outer surface of the buried electrical/electronic component excluding a portion connected to the connecting member and which are in close contact with a top and a bottom in the board thickness direction of the electrical/electronic component.

摘要翻译： 提供一种组件内置布线板及其制造方法，其能够进一步提高部件安装密度而不劣化可靠性。 所述部件内置布线板包括：导电层（34,35），其在所述板的厚度方向上延伸并埋设在所述板中而不暴露于所述板的上表面和下表面; 一个电子/电子部件（33），具有一个终端并埋设在该电路板中，该端子面向该掩埋的导电层; 设置在掩埋电气/电子部件的端子与导电层之间的间隙中的电连接/机械连接端子和导电层的连接件（36,37）; 以及两个覆盖所述埋入的电气/电子部件的外表面的绝缘层（11,15），除了连接到所述连接构件的部分之外，所述外部表面与所述电气/电子部件的板厚方向上的顶部和底部紧密接触 电气/电子部件。

公开(公告)号：US20070125985A1

公开(公告)日：2007-06-07

申请号：US11647368

申请日：2006-12-29

申请人： Hiroyuki Hirai ,  Keiko Sugihara ,  Junji Nishigaki

发明人： Hiroyuki Hirai ,  Keiko Sugihara ,  Junji Nishigaki

IPC分类号： C09K11/54 ,  C09K11/56

CPC分类号： C09K11/574 ,  B82Y15/00 ,  C09K11/584 ,  G01N33/582 ,  G01N33/588 ,  Y10S977/773 ,  Y10S977/786 ,  Y10S977/813 ,  Y10S977/824 ,  Y10S977/834 ,  Y10T428/2991

摘要： A doped-type metal sulfide phosphor nanoparticle dispersion, comprising a doped-type metal sulfide phosphor nanoparticle dispersed in water and/or a hydrophilic solvent, wherein the doped-type metal sulfide phosphor nanoparticle comprises a surface that is modified with a surface modifier, the surface modifier being a compound represented by formula [I]: HS-L-W   Formula [I] wherein L represents a divalent linking group; and W represents COOM or NH2, in which M represents a hydrogen atom, an alkali metal atom, or NX4, in which X represents a hydrogen atom or an alkyl group.

摘要翻译： 掺杂型金属硫化物荧光体纳米颗粒分散体，其包含分散在水中的掺杂型金属硫化物荧光体纳米颗粒和/或亲水性溶剂，其中所述掺杂型金属硫化物荧光体纳米颗粒包括用表面改性剂改性的表面， 表面改性剂是由式[I]表示的化合物：<？in-line-formula description =“In-line Formulas”end =“lead”？> HS-LW Formula [I] <？in-line-formula description = “In-line Formulas”end =“tail”？>其中L表示二价连接基团; W表示COOM或NH 2，其中M表示氢原子，碱金属原子或NX 4，其中X表示氢原子或烷基 。