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    Nucleic acid amplification device
    1.
    发明申请
    Nucleic acid amplification device 有权
    核酸扩增装置

    公开(公告)号：US20090215162A1

    公开(公告)日：2009-08-27

    申请号：US12292167

    申请日：2008-11-13

    申请人： Tomoharu Kajiyama ,  Masataka Shirai ,  Hideki Kambara

    发明人： Tomoharu Kajiyama ,  Masataka Shirai ,  Hideki Kambara

    IPC分类号： C12M1/40

    CPC分类号： B01L3/50851 ,  B01L3/5025 ,  B01L3/50857 ,  B01L2200/0668 ,  B01L2300/0819

    摘要： This invention provides a nucleic acid amplification device whereby the abundance of a target molecule can be maintained before and after a step of separately amplifying a sample such that highly accurate analysis results that can be applied to gene expression analysis can be obtained. Also, a nucleic acid amplification device having a structure in which a plurality of minute reaction cells each comprising a set of a bead-retaining space capable of retaining a single analysis bead and a reagent reaction space retaining no bead but having a volume that is large enough to cause a reagent reaction therein are positioned so as to form a planar face is provided.

    摘要翻译： 本发明提供了一种核酸扩增装置，其可以在分别扩增样品的步骤之前和之后维持目标分子的丰度，使得可以获得可应用于基因表达分析的高精度分析结果。 另外，具有这样的结构的核酸扩增装置，其中多个微小反应单元各自包含一组能够保留单个分析珠的珠保留空间和不保留珠而具有大体积的试剂反应空间 足以使其中的试剂反应定位成形成平面。

    CHEMILUMINESCENT DETECTION SYSTEM
    4.
    发明申请
    CHEMILUMINESCENT DETECTION SYSTEM 审中-公开
    荧光检测系统

    公开(公告)号：US20080260577A1

    公开(公告)日：2008-10-23

    申请号：US12032091

    申请日：2008-02-15

    申请人： Masataka SHIRAI ,  Tomoharu Kajiyama ,  Hideki Kambara

    发明人： Masataka SHIRAI ,  Tomoharu Kajiyama ,  Hideki Kambara

    IPC分类号： G01N21/76

    CPC分类号： G01N21/76 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2300/0816 ,  B01L2300/0877 ,  G01N21/01 ,  G01N21/251 ,  G01N21/763

    摘要： Throughput is improved by increasing the number of micro-reaction chambers. There is provided a chemiluminescent detection system that has a so-called plate on which many reaction chambers are one-dimensionally or two-dimensionally arranged, characterized in that optical detection is performed using a line or area sensor having many detection pixels, the spacing of the optical detection pixels substantially matches the spacing of the reaction chambers on the plate, and the micro-reaction chambers and the pixels are made to correspond one-to-one with each other so that light from the reaction chambers on the plate enters the detection pixels most efficiently and does not scatter to other pixels. To make the micro-reaction chambers arrayed on the plate and the pixels of the image pickup element plate correspond one-to-one with each other, light-emitting substances or reflectors or photoabsorption substances are placed so as to serve as alignment marks.

    摘要翻译： 通过增加微反应室的数量来改善生产能力。 提供一种化学发光检测系统，其具有所谓的板，其中许多反应室被一维或二维排列，其特征在于，使用具有许多检测像素的线或区域传感器进行光学检测， 光学检测像素基本上与板上的反应室的间隔相匹配，并且使微反应室和像素彼此一一对应，使得来自板上的反应室的光进入检测 像素最有效，不会散射到其他像素。 为了使微反应室排列在板上并且图像拾取元件板的像素彼此一一对应，放置发光物质或反射体或光吸收物质以作为对准标记。

    Methods for quantitative cDNA analysis in single-cell
    5.
    发明授权
    Methods for quantitative cDNA analysis in single-cell 有权
    单细胞定量cDNA分析方法

    公开(公告)号：US08802367B2

    公开(公告)日：2014-08-12

    申请号：US11783575

    申请日：2007-04-10

    申请人： Kiyomi Taniguchi ,  Hideki Kambara ,  Tomoharu Kajiyama

    发明人： Kiyomi Taniguchi ,  Hideki Kambara ,  Tomoharu Kajiyama

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/02 ,  C07H21/04

    CPC分类号： C12Q1/6811 ,  C12N15/1096 ,  C12Q1/6809 ,  C12Q1/6834 ,  C12Q2565/537 ,  C12Q2525/173 ,  C12Q2521/301 ,  C12Q2561/113

    摘要： It is an object to provide a method of suitably analyzing the amount of gene expression of a single-cell.A method of detecting a nucleic acid comprising a step of sampling a single-cell from a sample containing at least a single-cell, a cell lysis step of lysing cell membrane of the sampled single-cell and extracting nucleic acids from the cell, a DNase treatment step of degrading DNA of the extracted nucleic acids with DNase, a step of hybridizing mRNA of the total RNA contained in the single-cell with oligo (dT) fixed onto a carrier, a step of performing reverse transcription of the mRNA hybridized with the oligo (dT) to fix cDNA derived from the single-cell onto the carrier, thereby preparing a single-cell derived cDNA library fixed onto a carrier, and a step of amplifying cDNA fixed onto the carrier and simultaneously detecting an amplification amount of the cDNA.

    摘要翻译： 本发明的目的是提供适当分析单细胞的基因表达量的方法。 一种检测核酸的方法，包括从含有至少单细胞的样品中取样单细胞的步骤，裂解采样的单细胞的细胞膜并从细胞中提取核酸的细胞裂解步骤 用DNase降解提取的核酸的DNA的DNA酶处理步骤，将单细胞中含有的总RNA的mRNA与固定在载体上的寡聚（dT）杂交的步骤，将与mRNA杂交的mRNA进行逆转录的步骤 将来自单细胞的cDNA固定在载体上的寡核苷酸（dT），从而制备固定在载体上的单细胞衍生的cDNA文库，以及扩增固定在载体上的cDNA并同时检测扩增量的步骤 cDNA。

    Multiplex SNP typing by bioluminometric assay coupled with terminator incorporation
    6.
    发明申请
    Multiplex SNP typing by bioluminometric assay coupled with terminator incorporation 审中-公开
    通过生物光谱测定与终止子掺入进行复制SNP分型

    公开(公告)号：US20060240445A1

    公开(公告)日：2006-10-26

    申请号：US11209652

    申请日：2005-08-24

    申请人： Hideki Kambara ,  Guohua Zhou ,  Tomoharu Kajiyama

    发明人： Hideki Kambara ,  Guohua Zhou ,  Tomoharu Kajiyama

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6823 ,  C12Q1/6869 ,  C12Q2565/301 ,  C12Q2563/103 ,  C12Q2525/186

    摘要： The present invention provides a method for analyzing a nucleotide sequence comprising the steps of: carrying out complementary strand synthesis by adding at least one of four kinds of ddNTP corresponding to nucleotides A, G, T, and C, or derivatives thereof to a reaction vessel containing a nucleic acid sample to extend one nucleotide at a target site; performing a bioluminescent reaction with the use of ATP formed from released pyrophosphate as a reaction substrate; and typing the target site by determining the presence or absence of the complementary strand synthesis based on a result of the bioluminescent reaction. The method of the present invention allows multiplex SNPs to be typed in one reaction vessel

    摘要翻译： 本发明提供了分析核苷酸序列的方法，包括以下步骤：通过将对应于核苷酸A，G，T和C的四种ddNTP或其衍生物中的至少一种或其衍生物加入到反应容器中来进行互补链合成 含有核酸样品以在靶位点延伸一个核苷酸; 使用由释放的焦磷酸盐形成的ATP作为反应底物进行生物发光反应; 并通过基于生物发光反应的结果确定互补链合成的存在或不存在来分类靶位点。 本发明的方法允许在一个反应​​容器中输入多重SNP

    Luminescence detection apparatus
    7.
    发明申请
    Luminescence detection apparatus 审中-公开
    发光检测装置

    公开(公告)号：US20060141494A1

    公开(公告)日：2006-06-29

    申请号：US11209702

    申请日：2005-08-24

    申请人： Hideki Kambara ,  Tomoharu Kajiyama ,  Kunio Harada ,  Masao Kamahori

    发明人： Hideki Kambara ,  Tomoharu Kajiyama ,  Kunio Harada ,  Masao Kamahori

    IPC分类号： C12Q1/68 ,  C12M1/34

    CPC分类号： G01N21/03 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/76

    摘要： An object of the present invention is to provide a luminescence detection apparatus compact in size which is capable of conveniently determining DNA base sequences at a low cost. According to the present invention, a luminescence detection apparatus 1 is provided comprising: a plurality of reaction cells 6 each having a transparent bottom portion; a solution-dispensing portion 19 equipped with capillaries 18 positioned above the reaction cells 6 and put into a one-to-one correspondence with the reaction cells 6; and a light-detecting portion 29 having a plurality of light-sensing elements 24 put into a one-to-one correspondence with the reaction cells 6 and arranged in proximity to the bottom surfaces of the reaction cells 6, wherein the a plurality of light-sensing elements 24 of the light-detecting portion 29 detect respective luminescences in the reaction cells 6 generated by injecting reagent solutions from the solution-dispensing portion 19 to the reaction cells 6.

    摘要翻译： 本发明的目的是提供一种尺寸紧凑的发光检测装置，其能够以低成本方便地确定DNA碱基序列。 根据本发明，提供一种发光检测装置1，其包括：多个反应池6，每个反应池6具有透明底部; 溶液分配部分19，其配备有位于反应单元6上方并与反应单元6一一对应的毛细管18; 以及具有多个光检测元件24的光检测部分29，其与反应单元6一一对应地布置在反应单元6的底表面附近，其中多个光 光检测部分29的光敏元件24检测通过从溶液分配部分19将反应池6注入试剂溶液而产生的反应池6中的相应发光。

    Large-scale parallel nucleic acid analysis method
    8.
    发明申请
    Large-scale parallel nucleic acid analysis method 审中-公开
    大规模并行核酸分析方法

    公开(公告)号：US20080318244A1

    公开(公告)日：2008-12-25

    申请号：US12213448

    申请日：2008-06-19

    申请人： Hiroko Matsunaga ,  Hideki Kambara ,  Tomoharu Kajiyama

    发明人： Hiroko Matsunaga ,  Hideki Kambara ,  Tomoharu Kajiyama

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6834 ,  C12Q1/6844 ,  C12Q2537/143 ,  C12Q2525/191 ,  C12Q2533/101 ,  C12Q2565/537

    摘要： It is intended to provide a technique for amplifying, individually and in parallel, nucleic acids contained in a mixture of plural kinds of nucleic acid samples. The present invention provides a nucleic acid analysis method comprising amplification means, whereby amplification reaction is performed in a reaction solution comprising a homogeneous solvent and comprising at least plural template nucleic acids and solid phase carriers comprising one or more kinds of amplification probes immobilized on the surface, to prevent amplified products attributed to two or more template nucleic acids from being replicated in one solid phase carrier. According to the present invention, plural kinds of analyte nucleic acid samples in a mixed state can be amplified individually and in parallel. This method achieves one solid phase carrier-one nucleic acid. Therefore, a higher density of solid phase carriers with obtained amplified products is easily achieved, leading to improved throughput of amplified product analysis. Reactions in all the amplification reaction steps are performed under homogeneous solvent conditions. Therefore, the method of the present invention is performed by convenient procedures and as such, is suitable to automation.

    摘要翻译： 旨在提供用于扩增多种核酸样品的混合物中包含的核酸的单独和并行扩增的技术。 本发明提供了包含扩增方法的核酸分析方法，其中扩增反应在包含均相溶剂并包含至少多个模板核酸的反应溶液中进行，并且包含固定在表面上的一种或多种扩增探针的固相载体 ，以防止归因于两种或更多种模板核酸的扩增产物被复制在一种固相载体中。 根据本发明，可以单独并行地扩增处于混合状态的多种分析物核酸样品。 该方法实现了一种固相载体一核酸。 因此，容易获得具有获得的扩增产物的较高密度的固相载体，从而提高扩增产物分析的产量。 所有扩增反应步骤中的反应在均相溶剂条件下进行。 因此，本发明的方法通过方便的程序进行，因此适用于自动化。