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1.发明授权
公开(公告)号：US5527699A
公开(公告)日：1996-06-18
申请号：US396746
申请日：1995-03-01
IPC分类号： A23G3/00 , A23G3/34 , A23G3/38 , A23G3/42 , A23L1/09 , A23L1/187 , A23L1/218 , A23L1/236 , A61K8/04 , A61K8/34 , A61K8/60 , A61K8/73 , A61Q11/00 , A61Q17/04 , A61Q19/00 , A61Q19/02 , C08B37/00 , C12N9/26 , C12P19/14 , C12P19/20 , C13K13/00 , C12N1/00 , C12N1/12 , C12N9/28
CPC分类号： A23G3/42 , A23G3/346 , A23G3/38 , A23L19/20 , A23L27/34 , A23L27/35 , A23L29/35 , A23L9/12 , A61K8/60 , A61K8/732 , A61Q19/00 , C12N9/2411 , C12N9/2414 , C12P19/14 , C12R1/05 , C12Y302/01 , C13K13/00 , A23G2200/02 , A23G2200/06 , A61Q11/00 , A61Q17/04 , A61Q19/02 , Y10S435/829
摘要： An amylase is preferrably obtained from Alcaligenes latus FERM BP-4578. The amylase has an activity of mainly forming maltohexaose and maltoheptaose from starch, but does not substantially have an activity of hydrolyzing maltohexaose and an oligosaccharide having a molecular weight lower than maltohexaose. The amylase does not substantially act on maltoheptaose and has a molecular weight of about 43,000+3,000 daltons as measured by sodium dodecyl sulfate polyacrylamide gel electrophoresis. It has an isoelectric point of about 7.6+0.5 as measured on isoelectrophoresis using an ampholyte, an optimum pH of about 5.0 in the presence of calcium ion, an optimum temperature of about 70.degree. C. in the presence of calcium ion as measured in terms of relative enzyme activity, a pH stability of about 4.5-10.5 in the presence of calcium ion and a thermal stability up to about 60.degree. C. in the presence of calcium ion as measured in terms of residual enzyme activity. By using the amylase, saccharide compositions rich in maltohexaose and/or maltoheptaose or those rich in maltohexaitol and/or maltoheptaitol can be readily prepared in an industrial scale, and the saccharide compositions thus obtained can be used in a variety of food products, cosmetics and pharmaceuticals.
摘要翻译： 优选从产碱杆菌FERM BP-4578获得淀粉酶。 淀粉酶主要由淀粉形成麦芽六糖和麦芽庚糖，但实质上不具有水解麦芽六糖的活性和分子量低于麦芽六糖的寡糖。 淀粉酶基本上不作用于麦芽七糖，并且通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳测量，其分子量为约43,000±3,000道尔顿。 它具有约7.6±0.5的等电点，其在使用两性电解质的等电泳测量时，在钙离子存在下的最佳pH为约5.0，在钙离子存在下的最佳温度为约70℃ 相对酶活性，在钙离子存在下pH稳定性为约4.5-10.5，在钙离子存在下，在残余酶活性方面测定时，热稳定性高达约60℃。 通过使用淀粉酶，可以容易地以工业规模制备富含麦芽六糖和/或麦芽庚糖的富含麦芽六糖和/或麦芽庚糖醇的糖组合物，由此获得的糖组合物可用于各种食品，化妆品和 药品。
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2.发明授权Maltohexaose and maltoheptaose forming amylase, and its preparation and uses 失效麦芽六糖和麦芽庚糖形成淀粉酶及其制备和用途
公开(公告)号：US5846808A
公开(公告)日：1998-12-08
申请号：US837949
申请日：1997-04-28
IPC分类号： A23G3/00 , A23G3/34 , A23G3/38 , A23G3/42 , A23L1/09 , A23L1/187 , A23L1/218 , A23L1/236 , A61K8/04 , A61K8/34 , A61K8/60 , A61K8/73 , A61Q11/00 , A61Q17/04 , A61Q19/00 , A61Q19/02 , C08B37/00 , C12N9/26 , C12P19/14 , C12P19/20 , C13K13/00 , C12N1/20 , C12N9/28
CPC分类号： A23G3/42 , A23G3/346 , A23G3/38 , A23L19/20 , A23L27/34 , A23L27/35 , A23L29/35 , A23L9/12 , A61K8/60 , A61K8/732 , A61Q19/00 , C12N9/2411 , C12N9/2414 , C12P19/14 , C12R1/05 , C12Y302/01 , C13K13/00 , A23G2200/02 , A23G2200/06 , A61Q11/00 , A61Q17/04 , A61Q19/02 , Y10S435/829
摘要： Disclosed is a novel amylase which mainly forms maltohexaose and maltoheptaose when reacted with starch, but does not substantially hydrolyze maltohexaose and an oligosaccharide of lower molecular than maltohexaose. The amylase can be prepared from microorganisms of the genus Alcaligenes, and has a relatively-high optimum temperature and thermal stability, as well as a relatively-wide range of optimum pH and pH stability. By using the amylase, saccharide compositions rich in maltohexaose and/or maltoheptaose or those rich on maltohexaitol and/or maltoheptaitol can be readily prepared in an industrial scale, and the saccharide compositions thus obtained can be used in a variety of food products, cosmetics and pharmaceuticals.
摘要翻译： 公开了一种新型淀粉酶，当与淀粉反应时主要形成麦芽六糖和麦芽庚糖，但基本上不水解麦芽六糖和低分子量比麦芽六糖的寡糖。 淀粉酶可以由产碱杆菌属的微生物制备，并且具有相对较高的最佳温度和热稳定性，以及相对宽范围的最佳pH和pH稳定性。 通过使用淀粉酶，可以容易地以工业规模制备富含麦芽六糖和/或麦芽七糖的富含麦芽六糖和/或麦芽庚糖的富含糖的组合物，并且由此获得的糖组合物可用于各种食品，化妆品和 药品。
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公开(公告)号：US5739024A
公开(公告)日：1998-04-14
申请号：US526082
申请日：1995-09-11
IPC分类号： A23G3/00 , A23G3/34 , A23G3/38 , A23G3/42 , A23L1/09 , A23L1/187 , A23L1/218 , A23L1/236 , A61K8/04 , A61K8/34 , A61K8/60 , A61K8/73 , A61Q11/00 , A61Q17/04 , A61Q19/00 , A61Q19/02 , C08B37/00 , C12N9/26 , C12P19/14 , C12P19/20 , C13K13/00 , C12N1/00 , C12N1/12 , C12N9/28
CPC分类号： A23G3/42 , A23G3/346 , A23G3/38 , A23L19/20 , A23L27/34 , A23L27/35 , A23L29/35 , A23L9/12 , A61K8/60 , A61K8/732 , A61Q19/00 , C12N9/2411 , C12N9/2414 , C12P19/14 , C12R1/05 , C12Y302/01 , C13K13/00 , A23G2200/02 , A23G2200/06 , A61Q11/00 , A61Q17/04 , A61Q19/02 , Y10S435/829
摘要： Disclosed is a novel amylase which mainly forms maltohexaose and maltoheptaose when acts on starch, but does not substantially hydrolyze maltohexaose and a lower molecular oligosaccharide than maltohexaose. The amylase can be prepared from microorganisms of the genus Alcaligenes, and has a relatively-high optimum temperature and thermal stability, as well as a relatively-wide range of optimum pH and pH stability. By using the amylase, saccharide compositions rich in maltohexaose and/or maltoheptaose or those rich in maltohexaitol and/or maltoheptaitol can be readily prepared in an industrial scale, and the saccharide compositions thus obtained can be used in a variety of food products, cosmetics and pharmaceuticals.
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4.发明授权Maltohexaose and maltoheptaose-forming amylase, and its preparation and uses 失效麦芽六糖和形成麦芽庚糖的淀粉酶及其制备和用途公开(公告)号：US06242224B1
公开(公告)日：2001-06-05
申请号：US08848661
申请日：1997-04-29
IPC分类号： C12P1914
CPC分类号： A23G3/42 , A23G3/346 , A23G3/38 , A23G2200/02 , A23G2200/06 , A23L9/12 , A23L19/20 , A23L27/34 , A23L27/35 , A23L29/35 , A61K8/60 , A61K8/732 , A61Q11/00 , A61Q17/04 , A61Q19/00 , A61Q19/02 , C12N9/2411 , C12N9/2414 , C12P19/14 , C12R1/05 , C12Y302/01 , C13K13/00 , Y10S435/829
摘要： Disclosed is a novel amylase which mainly forms maltohexaose and maltoheptaose when acts on starch, but does not substantially hydrolyze maltohexaose and a lower molecular oligosaccharide than maltohexaose. The amylase can be prepared from microorganisms of the genus Alcaligenes, and has a relatively-high optimum temperature and thermal stability, as well as a relatively-wide range of optimum pH and pH stability. By using the amylase, saccharide compositions rich in maltohexaose and/or maltoheptaose or those rich in maltohexaitol and/or maltoheptaitol can be readily prepared in an industrial scale, and the saccharide compositions thus obtained can be used in a variety of food products, cosmetics and pharmaceuticals.
摘要翻译： 公开了一种新的淀粉酶，当作用于淀粉时，主要形成麦芽六糖和麦芽庚糖，但不比麦芽六糖大大水解麦芽六糖和低分子寡糖。 淀粉酶可以由产碱杆菌属的微生物制备，并且具有相对较高的最佳温度和热稳定性，以及相对宽范围的最佳pH和pH稳定性。 通过使用淀粉酶，可以容易地以工业规模制备富含麦芽六糖和/或麦芽庚糖的富含麦芽六糖和/或麦芽庚糖醇的糖组合物，由此获得的糖组合物可用于各种食品，化妆品和 药品。
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5.发明授权Maltohexaose and maltoheptaose-forming amylase, and its preparation and uses 失效麦芽六糖和形成麦芽庚糖的淀粉酶及其制备和用途
公开(公告)号：US6107348A
公开(公告)日：2000-08-22
申请号：US848750
申请日：1997-05-01
IPC分类号： A23G3/00 , A23G3/34 , A23G3/38 , A23G3/42 , A23L1/09 , A23L1/187 , A23L1/218 , A23L1/236 , A61K8/04 , A61K8/34 , A61K8/60 , A61K8/73 , A61Q11/00 , A61Q17/04 , A61Q19/00 , A61Q19/02 , C08B37/00 , C12N9/26 , C12P19/14 , C12P19/20 , C13K13/00 , A61K47/36 , C12P19/04
CPC分类号： A23G3/42 , A23G3/346 , A23G3/38 , A23L19/20 , A23L27/34 , A23L27/35 , A23L29/35 , A23L9/12 , A61K8/60 , A61K8/732 , A61Q19/00 , C12N9/2411 , C12N9/2414 , C12P19/14 , C12R1/05 , C12Y302/01 , C13K13/00 , A23G2200/02 , A23G2200/06 , A61Q11/00 , A61Q17/04 , A61Q19/02 , Y10S435/829
摘要： Disclosed is a novel amylase which mainly forms maltohexaose and maltoheptaose when acts on starch, but does not substantially hydrolyze maltohexaose and a lower molecular oligosaccharide than maltohexaose. The amylase can be prepared from microorganisms of the genus Alcaligenes, and has a relatively-high optimum temperature and thermal stability, as well as a relatively-wide range of optimum pH and pH stability. By using the amylase, saccharide compositions rich in maltohexaose and/or maltoheptaose or those rich in maltohexaitol and/or maltoheptaitol can be readily prepared in an industrial scale, and the saccharide compositions thus obtained can be used in a variety of food products, cosmetics and pharmaceuticals.
摘要翻译： 公开了一种新的淀粉酶，当作用于淀粉时，主要形成麦芽六糖和麦芽庚糖，但不比麦芽六糖大大水解麦芽六糖和较低分子寡糖。 淀粉酶可以由产碱杆菌属的微生物制备，并且具有相对较高的最佳温度和热稳定性，以及相对宽范围的最佳pH和pH稳定性。 通过使用淀粉酶，可以容易地以工业规模制备富含麦芽六糖和/或麦芽庚糖的富含麦芽六糖和/或麦芽庚糖醇的糖组合物，由此获得的糖组合物可用于各种食品，化妆品和 药品。
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公开(公告)号：US5883243A
公开(公告)日：1999-03-16
申请号：US797248
申请日：1997-02-07
摘要： .alpha.-Isomaltosyl .alpha.-isomaltoside represented by O-.alpha.-D-glucopyranosyl-(1.fwdarw.6)-.alpha.-D-glucopyranosyl O-.alpha.-D-glucopyranosyl-(1.fwdarw.6)-.alpha.-D-glucopyranoside is a novel non-reducing saccharide which promotes the growth of bifid bacteria, has an anti-caries activity, and promotes the mineral absorption. Similarly as the non-reducing saccharide, .alpha.-isomaltosyl .alpha.-glucoside and .alpha.-isomaltotriosyl .alpha.-glucoside can be also used to prevent caries and promote the growth of bifid bacteria and the mineral absorption. These saccharides can be used alone or in combination in a variety of foods, cosmetics and pharmaceuticals.
摘要翻译： 由α-α-D-吡喃葡萄糖基 - （1-> 6）-β-D-吡喃葡萄糖基O-α-D-吡喃葡萄糖基 - （1-> 6）-β-D-吡喃葡萄糖苷代表的α-异麦角糖型α-异麦芽糖苷是 促进双歧杆菌生长的新型非还原性糖类具有抗龋齿活性，促进矿物质吸收。 类似地，作为非还原性糖，α-异麦芽糖基α-葡萄糖苷和α-异麦芽糖基α-葡糖苷也可用于预防龋齿并促进双歧杆菌的生长和矿物质吸收。 这些糖类可以单独使用，也可以组合使用于各种食品，化妆品，药品等。
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7.发明授权
公开(公告)号：US5714368A
公开(公告)日：1998-02-03
申请号：US466434
申请日：1995-06-06
CPC分类号： C12P19/14 , C12N9/2428 , C12Y302/01003
摘要： Disclosed are novel thermostable non-reducing saccharides-forming enzyme, its preparation and uses. The enzyme is obtainable from the culture of microorganisms such as Sulfolobus acidocaldarius (ATCC 33909 and ATCC 49426) and Sulfolobus solfataricus (ATCC 35091 and ATCC 35092), and capable of forming non-reducing saccharides having a trehalose structure as an end unit when allowed to act on reducing partial starch hydrolysates at a temperature of over 55.degree. C. Glucoamylase and .alpha.-glucosidase readily yield trehalose when allowed to act on the non-reducing saccharides. These non-reducing saccharides and trehalose are extensively useful in food products, cosmetics and pharmaceuticals.
摘要翻译： 公开了新型热稳定性非还原性糖形成酶，其制备和用途。 该酶可以从微生物的培养物获得，例如硫酸亚硫酸杆菌（ATCC 33909和ATCC 49426）和溶血糠鱼（Sulfolobus solfataricus）（ATCC 35091和ATCC 35092），并且能够形成具有海藻糖结构的非还原性糖作为终端单元 在超过55℃的温度下还原部分淀粉水解产物起作用。当允许作用于非还原糖时，葡糖淀粉酶和α-葡糖苷酶容易产生海藻糖。 这些非还原性糖和海藻糖广泛用于食品，化妆品和药物。
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8.发明授权
公开(公告)号：US6090792A
公开(公告)日：2000-07-18
申请号：US975917
申请日：1997-11-21
IPC分类号： A23C9/13 , A21D2/18 , A23C9/18 , A23G3/00 , A23G3/02 , A23G3/34 , A23G3/42 , A23G3/48 , A23G4/00 , A23G4/10 , A23L1/202 , A23L1/236 , A23L1/31 , A23L1/314 , A23L1/32 , A23L2/39 , A23L2/64 , A61K8/00 , A61K8/365 , A61K8/60 , A61K8/66 , A61K8/73 , A61K8/99 , A61K9/06 , A61K9/08 , A61K9/20 , A61K9/36 , A61K31/70 , A61K31/7016 , A61Q7/00 , A61Q11/00 , A61Q17/04 , A61Q19/00 , A61Q19/02 , C07H3/04 , C12N1/20 , C12N9/10 , C12N9/90 , C12P19/12 , C12P19/14 , C12P19/16 , C12P19/20 , C12P19/24 , C12R1/01 , C12R1/40 , C13K13/00
CPC分类号： A23G3/42 , A23G3/343 , A23G3/346 , A23G4/10 , A23L13/428 , A23L15/20 , A23L2/64 , A23L27/33 , A61K8/365 , A61K8/60 , A61K8/66 , A61Q11/00 , A61Q19/00 , C12N9/90 , C12P19/16 , C12P19/20 , C12P19/24 , C12R1/01 , C12R1/40 , C13K13/00 , A23G2200/02 , A23G2200/06 , A61Q17/04 , A61Q19/02 , A61Q7/00 , Y10S435/877
摘要： An enzyme, which has a molecular weight of about 57,000-120,000 daltons on SDS-PAGE and a pI of about 3.8-5.1 on isoelectrophoresis using ampholyte, converts maltose into trehalose and vice versa. The enzyme was isolated from microorganisms of the genera Pimelobacter, Pseudomonas and Thermus. By using the enzyme, trehalose is readily formed from a commercially available maltose in an industrial scale and a relatively-low cost. Trehalose and saccharide compositions containing the same, which are preparable with the enzyme, are suitably used in food products, cosmetic compositions and pharmaceutical compositions.
摘要翻译： 在SDS-PAGE上具有约57,000-120,000道尔顿分子量的酶，使用两性电解质在等电泳上的pI约为3.8-5.1，将麦芽糖转化为海藻糖，反之亦然。 从Pimelobacter，Pseudomonas和Thermus属的微生物中分离酶。 通过使用该酶，由工业规模的市售麦芽糖容易地形成海藻糖并且成本相对较低。 适用于食品，化妆品组合物和药物组合物的含有该酶的海藻糖和糖组合物可以用酶制备。
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公开(公告)号：US5759610A
公开(公告)日：1998-06-02
申请号：US815679
申请日：1997-03-12
IPC分类号： A21D2/18 , A23C1/03 , A23C9/13 , A23C9/156 , A23G1/00 , A23G1/40 , A23G3/00 , A23G3/34 , A23G3/42 , A23G4/00 , A23G4/10 , A23K1/16 , A23L1/187 , A23L1/20 , A23L1/236 , A23L1/30 , A23L1/314 , A23L1/32 , A23L2/60 , A61K8/60 , A61K31/70 , A61Q7/00 , A61Q11/00 , A61Q19/00 , C07H3/04 , C12N9/90 , C12P19/12 , C12P19/18 , C13K13/00 , C07H3/00 , C12N9/24
CPC分类号： A23G4/10 , A21D2/181 , A23C9/1307 , A23C9/156 , A23G1/40 , A23G3/346 , A23G3/42 , A23K20/163 , A23L11/05 , A23L13/428 , A23L15/30 , A23L2/60 , A23L27/33 , A23L9/10 , A23L9/12 , A61K8/60 , A61Q19/00 , C07H3/04 , C12N9/90 , C12P19/12 , C12P19/18 , C12Y504/99016 , C13K13/00 , A23G2200/02 , A23G2200/06 , A61Q11/00 , A61Q19/02 , A61Q7/00 , Y10S435/874 , Y10S435/876
摘要： Microorganisms which are able to produce maltose/trehalose conversion enzyme, a novel enzyme, are cultivated in nutrient culture media with malose. During the cultivation, the microorganisms readily convert maltose into trehalose to accumulate trehalose in the cultures which yield saccharide mixtures with high trehalose contents when separated from insoluble substances. Removal of contaminant saccharides and subsequent crystallization readily yield trehalose in crystalline trehalose hydrate or anhydrous crystalline form. The trehalose and saccharide mixture containing the same commonly bear desirable properties including mild sweetness and superior stability which render them very useful in a variety of compositions indlucing food products, cosmetics and medicines.
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公开(公告)号：US5965411A
公开(公告)日：1999-10-12
申请号：US964338
申请日：1997-11-04
IPC分类号： A23C9/13 , A21D2/18 , A23C9/18 , A23G3/00 , A23G3/02 , A23G3/34 , A23G3/42 , A23G3/48 , A23G4/00 , A23G4/10 , A23L1/202 , A23L1/236 , A23L1/31 , A23L1/314 , A23L1/32 , A23L2/39 , A23L2/64 , A61K8/00 , A61K8/365 , A61K8/60 , A61K8/66 , A61K8/73 , A61K8/99 , A61K9/06 , A61K9/08 , A61K9/20 , A61K9/36 , A61K31/70 , A61K31/7016 , A61Q7/00 , A61Q11/00 , A61Q17/04 , A61Q19/00 , A61Q19/02 , C07H3/04 , C12N1/20 , C12N9/10 , C12N9/90 , C12P19/12 , C12P19/14 , C12P19/16 , C12P19/20 , C12P19/24 , C12R1/01 , C12R1/40 , C13K13/00
CPC分类号： A23G3/42 , A23G3/343 , A23G3/346 , A23G4/10 , A23L13/428 , A23L15/20 , A23L2/64 , A23L27/33 , A61K8/365 , A61K8/60 , A61K8/66 , A61Q11/00 , A61Q19/00 , C12N9/90 , C12P19/16 , C12P19/20 , C12P19/24 , C12R1/01 , C12R1/40 , C13K13/00 , A23G2200/02 , A23G2200/06 , A61Q17/04 , A61Q19/02 , A61Q7/00 , Y10S435/877
摘要： An enzyme, which has a molecular weight of about 57,000-120,000 daltons on SDS-PAGE and a pI of about 3.8-5.1 on isoelectrophoresis using ampholyte, converts maltose into trehalose and vice versa. The enzyme was isolated from microorganisms of the genera Pimelobacter, Pseudomonas and Thermus. By using the enzyme, trehalose is readily formed from a commercially available maltose in an industrial scale and a relatively-low cost. Trehalose and saccharide compositions containing the same, which are preparable with the enzyme, are suitably used in food products, cosmetic compositions and pharmaceutical compositions.
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