公开(公告)号：US20140120604A1

公开(公告)日：2014-05-01

申请号：US14110635

申请日：2012-04-06

申请人： Mauro Aguanno ,  Brian Barrett ,  Brian Chawke ,  Kieran Curran ,  Damian Curtin ,  Tara Dalton ,  Mark Davies ,  Xiaona Hou ,  David Kinahan ,  Damien King ,  Mark Korenke ,  Conor McCarthy ,  David McGuire ,  Michael Sayers ,  Noel Sirr ,  Ryan J. Talbot

发明人： Mauro Aguanno ,  Brian Barrett ,  Brian Chawke ,  Kieran Curran ,  Damian Curtin ,  Tara Dalton ,  Mark Davies ,  Xiaona Hou ,  David Kinahan ,  Damien King ,  Mark Korenke ,  Conor McCarthy ,  David McGuire ,  Michael Sayers ,  Noel Sirr ,  Ryan J. Talbot

IPC分类号： G01N21/64 ,  B01F13/00

CPC分类号： C12Q1/686 ,  B01F13/0005 ,  B01F13/0071 ,  B01F15/00214 ,  B01L3/502715 ,  B01L3/502784 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/143 ,  B01L2300/06 ,  B01L2300/0654 ,  B01L2300/0867 ,  G01N21/6428 ,  G01N35/00623 ,  G01N2021/6439 ,  G01N2021/6441 ,  G01N2035/00465 ,  G01N2201/062

摘要： Provided herein is a biological detection system and method of use wherein the biological detection system comprises at least one mixer or liquid bridge for combining at least two liquid droplets and an error correction system for detecting whether or not proper mixing or combining of the two component droplets have occurred.

摘要翻译： 本文提供了一种生物检测系统和使用方法，其中生物检测系统包括用于组合至少两个液滴的至少一个混合器或液体桥，以及用于检测是否适当混合或组合两个组分液滴的误差校正系统 已经发生

公开(公告)号：US07993911B2

公开(公告)日：2011-08-09

申请号：US12097455

申请日：2007-02-07

申请人： Mark Davies ,  Tara Dalton ,  Julie Garvey ,  Kieran Curran ,  Damian Curtin

发明人： Mark Davies ,  Tara Dalton ,  Julie Garvey ,  Kieran Curran ,  Damian Curtin

IPC分类号： C12M1/34

CPC分类号： B01J19/0046 ,  B01F13/0071 ,  B01F15/0201 ,  B01F15/0232 ,  B01J2219/00313 ,  B01J2219/00353 ,  B01J2219/00479 ,  B01J2219/00587 ,  B01J2219/00608 ,  B01L3/5025 ,  B01L3/502784 ,  B01L2200/0673 ,  B01L2300/0829 ,  B01L2400/0487 ,  Y10T436/25

摘要： A multi-port liquid bridge (1) adds aqueous phase droplets (10) in an enveloping oil phase carrier liquid (11) to a draft channel (4, 6). A chamber (3) links four ports, and it is permanently full of oil (11) when in use. Oil phase is fed in a draft flow from an inlet port (4) and exits through a draft exit port (6) and a compensating flow port (7). The oil carrier and the sample droplets (3) (“aqueous phase”) flow through the inlet port (5) with an equivalent fluid flow subtracted through the compensating port (7). The ports of the bridge (1) are formed by the ends of capillaries held in position in plastics housings. The phases are density matched to create an environment where gravitational forces are negligible. This results in droplets (10) adopting spherical forms when suspended from capillary tube tips. Furthermore, the equality of mass flow is equal to the equality of volume flow. The phase of the inlet flow (from the droplet inlet port (5) and the draft inlet port (4) is used to determine the outlet port (6) flow phase.

摘要翻译： 多端口液体桥（1）将包含油相载体液体（11）中的水相液滴（10）加入到通风道（4,6）。 室（3）连接四个端口，在使用时永久充满油（11）。 油相在进气口（4）的进料流中进料，并通过排气口（6）和补偿流通口（7）排出。 油载体和样品液滴（“水相”）以相当的流体流通过入口端口（5）流过补偿口（7）。 桥梁（1）的端口由保持在塑料壳体中的位置的毛细管的端部形成。 这些阶段是密度匹配的，以创造引力几乎可忽略的环境。 这导致当从毛细管尖端悬浮时，液滴（10）采用球形。 此外，质量流量的相等性等于体积流量的相等。 入口流（从液滴入口端口5和进气口4）的相位用于确定出口（6）的流动相位。

公开(公告)号：US20100015606A1

公开(公告)日：2010-01-21

申请号：US12097455

申请日：2007-02-07

申请人： Mark Davies ,  Tara Dalton ,  Julie Garvey ,  Kieran Curran ,  Damian Curtin

发明人： Mark Davies ,  Tara Dalton ,  Julie Garvey ,  Kieran Curran ,  Damian Curtin

IPC分类号： C12Q1/68 ,  B01L3/00 ,  G01N1/00

CPC分类号： B01J19/0046 ,  B01F13/0071 ,  B01F15/0201 ,  B01F15/0232 ,  B01J2219/00313 ,  B01J2219/00353 ,  B01J2219/00479 ,  B01J2219/00587 ,  B01J2219/00608 ,  B01L3/5025 ,  B01L3/502784 ,  B01L2200/0673 ,  B01L2300/0829 ,  B01L2400/0487 ,  Y10T436/25

摘要： A multi-port liquid bridge (1) adds aqueous phase droplets (10) in an enveloping oil phase carrier liquid (11) to a draft channel (4, 6). A chamber (3) links four ports, and it is permanently full of oil (11) when in use. Oil phase is fed in a draft flow from an inlet port (4) and exits through a draft exit port (6) and a compensating flow port (7). The oil carrier and the sample droplets (3) (“aqueous phase”) flow through the inlet port (5) with an equivalent fluid flow subtracted through the compensating port (7). The ports of the bridge (1) are formed by the ends of capillaries held in position in plastics housings. The phases are density matched to create an environment where gravitational forces are negligible. This results in droplets (10) adopting spherical forms when suspended from capillary tube tips. Furthermore, the equality of mass flow is equal to the equality of volume flow. The phase of the inlet flow (from the droplet inlet port (5) and the draft inlet port (4) is used to determine the outlet port (6) flow phase.

摘要翻译： 多端口液体桥（1）将包含油相载体液体（11）中的水相液滴（10）加入到通风道（4,6）。 室（3）连接四个端口，在使用时永久充满油（11）。 油相在进气口（4）的进料流中进料，并通过排气口（6）和补偿流通口（7）排出。 油载体和样品液滴（“水相”）以相当的流体流通过入口端口（5）流过补偿口（7）。 桥梁（1）的端口由保持在塑料壳体中的位置的毛细管的端部形成。 这些阶段是密度匹配的，以创造引力几乎可忽略的环境。 这导致当从毛细管尖端悬浮时，液滴（10）采用球形。 此外，质量流量的相等性等于体积流量的相等。 入口流（从液滴入口端口5和进气口4）的相位用于确定出口（6）的流动相位。

公开(公告)号：US08563244B2

公开(公告)日：2013-10-22

申请号：US13177437

申请日：2011-07-06

申请人： Mark Davies ,  Tara Dalton ,  Julie Garvey ,  Kieran Curran ,  Damian Curtin

发明人： Mark Davies ,  Tara Dalton ,  Julie Garvey ,  Kieran Curran ,  Damian Curtin

IPC分类号： C12Q1/68 ,  C12M1/34

CPC分类号： B01J19/0046 ,  B01F13/0071 ,  B01F15/0201 ,  B01F15/0232 ,  B01J2219/00313 ,  B01J2219/00353 ,  B01J2219/00479 ,  B01J2219/00587 ,  B01J2219/00608 ,  B01L3/5025 ,  B01L3/502784 ,  B01L2200/0673 ,  B01L2300/0829 ,  B01L2400/0487 ,  Y10T436/25

摘要： A multi-port liquid bridge (1) adds aqueous phase droplets (10) in an enveloping oil phase carrier liquid (11) to a draft channel (4, 6). A chamber (3) links four ports, and it is permanently full of oil (11) when in use. Oil phase is fed in a draft flow from an inlet port (4) and exits through a draft exit port (6) and a compensating flow port (7). The oil carrier and the sample droplets (3) (“aqueous phase”) flow through the inlet port (5) with an equivalent fluid flow subtracted through the compensating port (7). The ports of the bridge (1) are formed by the ends of capillaries held in position in plastics housings. The phases are density matched to create an environment where gravitational forces are negligible. This results in droplets (10) adopting spherical forms when suspended from capillary tube tips. Furthermore, the equality of mass flow is equal to the equality of volume flow. The phase of the inlet flow (from the droplet inlet port (5) and the draft inlet port (4) is used to determine the outlet port (6) flow phase.

摘要翻译： 多端口液体桥（1）将包含油相载体液体（11）中的水相液滴（10）加入到通风道（4,6）。 室（3）连接四个端口，在使用时永久充满油（11）。 油相在进气口（4）的进料流中进料，并通过排气口（6）和补偿流通口（7）排出。 油载体和样品液滴（“水相”）以相当的流体流通过入口端口（5）流过补偿口（7）。 桥梁（1）的端口由保持在塑料壳体中的位置的毛细管的端部形成。 这些阶段是密度匹配的，以创造引力几乎可忽略的环境。 这导致当从毛细管尖端悬浮时，液滴（10）采用球形。 此外，质量流量的相等性等于体积流量的相等。 入口流（从液滴入口端口5和进气口4）的相位用于确定出口（6）的流动相位。

公开(公告)号：US20120003658A1

公开(公告)日：2012-01-05

申请号：US13177437

申请日：2011-07-06

申请人： Mark DAVIES ,  Tara Dalton ,  Julie Garvey ,  Kieran Curran ,  Damian Curtin

发明人： Mark DAVIES ,  Tara Dalton ,  Julie Garvey ,  Kieran Curran ,  Damian Curtin

IPC分类号： C12Q1/68

CPC分类号： B01J19/0046 ,  B01F13/0071 ,  B01F15/0201 ,  B01F15/0232 ,  B01J2219/00313 ,  B01J2219/00353 ,  B01J2219/00479 ,  B01J2219/00587 ,  B01J2219/00608 ,  B01L3/5025 ,  B01L3/502784 ,  B01L2200/0673 ,  B01L2300/0829 ,  B01L2400/0487 ,  Y10T436/25

摘要： A multi-port liquid bridge (1) adds aqueous phase droplets (10) in an enveloping oil phase carrier liquid (11) to a draft channel (4, 6). A chamber (3) links four ports, and it is permanently full of oil (11) when in use. Oil phase is fed in a draft flow from an inlet port (4) and exits through a draft exit port (6) and a compensating flow port (7). The oil carrier and the sample droplets (3) (“aqueous phase”) flow through the inlet port (5) with an equivalent fluid flow subtracted through the compensating port (7). The ports of the bridge (1) are formed by the ends of capillaries held in position in plastics housings. The phases are density matched to create an environment where gravitational forces are negligible. This results in droplets (10) adopting spherical forms when suspended from capillary tube tips. Furthermore, the equality of mass flow is equal to the equality of volume flow. The phase of the inlet flow (from the droplet inlet port (5) and the draft inlet port (4) is used to determine the outlet port (6) flow phase.

摘要翻译： 多端口液体桥（1）将包含油相载体液体（11）中的水相液滴（10）加入到通风道（4,6）。 室（3）连接四个端口，在使用时永久充满油（11）。 油相在进气口（4）的进料流中进料，并通过排气口（6）和补偿流通口（7）排出。 油载体和样品液滴（“水相”）以相当的流体流通过入口端口（5）流过补偿口（7）。 桥梁（1）的端口由保持在塑料壳体中的位置的毛细管的端部形成。 这些阶段是密度匹配的，以创造引力几乎可忽略的环境。 这导致当从毛细管尖端悬浮时，液滴（10）采用球形。 此外，质量流量的相等性等于体积流量的相等。 入口流（从液滴入口端口5和进气口4）的相位用于确定出口（6）的流动相位。

公开(公告)号：US20100304443A1

公开(公告)日：2010-12-02

申请号：US12732494

申请日：2010-03-26

申请人： Mark Davies ,  Tara Dalton ,  Michael Sayers ,  Brian Chawke

发明人： Mark Davies ,  Tara Dalton ,  Michael Sayers ,  Brian Chawke

IPC分类号： C12P19/34 ,  C12M1/26

CPC分类号： B01L3/021 ,  B01L3/0293 ,  B01L7/52 ,  B01L2200/0642 ,  B01L2200/0673 ,  Y10T436/25 ,  Y10T436/25625

摘要： The present invention generally relates to devices, systems, and methods for acquiring and/or dispensing a sample without introducing a gas into a microfluidic system, such as a liquid bridge system. An exemplary embodiment provides a sampling device including an outer sheath; a plurality of tubes within the sheath, in which at least one of the tubes acquires a sample, and at least one of the tubes expels a fluid that is immiscible with the sample, in which the at least one tube that acquires the sample is extendable beyond a distal end of the sheath and retractable to within the sheath; and a valve connected to a distal portion of the sheath, in which the valve opens when the tube extends beyond the distal end and closes when the tube retracts to within the sheath.

摘要翻译： 本发明一般涉及用于获取和/或分配样品而不将气体引入诸如液体桥系统的微流体系统中的装置，系统和方法。 示例性实施例提供了一种包括外护套的取样装置; 鞘内的多个管，其中至少一个管获取样品，并且至少一个管排出与样品不混溶的流体，其中获取样品的至少一个管可扩展 超出护套的远端并伸缩到护套内; 以及连接到所述护套的远端部分的阀，当所述管延伸超过所述远端时所述阀打开，并且当所述管缩回到所述护套内时所述阀闭合。

公开(公告)号：US08741660B2

公开(公告)日：2014-06-03

申请号：US12732494

申请日：2010-03-26

申请人： Mark Davies ,  Tara Dalton ,  Michael Sayers ,  Brian Chawke

发明人： Mark Davies ,  Tara Dalton ,  Michael Sayers ,  Brian Chawke

IPC分类号： G01N1/18

CPC分类号： B01L3/021 ,  B01L3/0293 ,  B01L7/52 ,  B01L2200/0642 ,  B01L2200/0673 ,  Y10T436/25 ,  Y10T436/25625

摘要： The present invention generally relates to devices, systems, and methods for acquiring and/or dispensing a sample without introducing a gas into a microfluidic system, such as a liquid bridge system. An exemplary embodiment provides a sampling device including an outer sheath; a plurality of tubes within the sheath, in which at least one of the tubes acquires a sample, and at least one of the tubes expels a fluid that is immiscible with the sample, in which the at least one tube that acquires the sample is extendable beyond a distal end of the sheath and retractable to within the sheath; and a valve connected to a distal portion of the sheath, in which the valve opens when the tube extends beyond the distal end and closes when the tube retracts to within the sheath.

摘要翻译： 本发明一般涉及用于获取和/或分配样品而不将气体引入诸如液体桥系统的微流体系统中的装置，系统和方法。 示例性实施例提供了一种包括外护套的取样装置; 鞘内的多个管，其中至少一个管获取样品，并且至少一个管排出与样品不混溶的流体，其中获取样品的至少一个管可扩展 超出护套的远端并伸缩到护套内; 以及连接到所述护套的远端部分的阀，当所述管延伸超过所述远端时所述阀打开，并且当所述管缩回到所述护套内时所述阀闭合。

公开(公告)号：US20080277494A1

公开(公告)日：2008-11-13

申请号：US12092261

申请日：2006-02-07

申请人： Mark Davies ,  Tara Dalton ,  Kieran Curran

发明人： Mark Davies ,  Tara Dalton ,  Kieran Curran

IPC分类号： B29C45/20

CPC分类号： B01L3/502784 ,  B01F13/0071 ,  B01F15/0201 ,  B01F15/0232 ,  B01F2215/0037 ,  B01L3/5025 ,  B01L3/502715 ,  B01L3/502746 ,  B01L7/525 ,  B01L2200/0673 ,  B01L2300/0829 ,  B01L2300/0838 ,  B01L2400/0478 ,  B01L2400/0487 ,  B01L2400/0633 ,  B01L2400/082 ,  C12Q1/686 ,  F17D1/08 ,  Y10T137/87676 ,  Y10T436/117497 ,  Y10T436/118339 ,  Y10T436/2575

摘要： A bridge (30) comprises a first inlet port (31) at the end of a capillary, a narrower second inlet port (32) which is an end of a capillary, an outlet port (33) which is an end of a capillary, and a chamber (34) for silicone oil. The oil is density-matched with the reactor droplets such that a neutrally buoyant environment is created within the chamber (34). The oil within the chamber is continuously replenished by the oil separating the reactor droplets. This causes the droplets to assume a stable capillary-suspended spherical form upon entering the chamber (34). The spherical shape grows until large enough to span the gap between the ports, forming an axisymmetric liquid bridge. The introduction of a second droplet from the second inlet port (32) causes the formation of an unstable funicular bridge that quickly ruptures from the, finer, second inlet port (32), and the droplets combine at the liquid bridge (30). In another embodiment, a droplet (55) segments into smaller droplets which bridge the gap between the inlet and outlet ports.

摘要翻译： 桥（30）包括在毛细管端部的第一入口（31），作为毛细管端部的较窄的第二入口（32），作为毛细管的端部的出口（33） 和用于硅油的室（34）。 油与反应器液滴密度匹配，使得在室（34）内产生中性浮力的环境。 室内的油通过分离反应器液滴的油不断补充。 这使得液滴在进入室（34）时呈现稳定的毛细管悬浮球形。 球形增长直到足够大以跨越端口之间的间隙，形成轴对称液桥。 从第二入口端口（32）引入第二液滴导致形成不稳定的索道桥，其从较细的第二入口（32）快速破裂，并且液滴在液体桥（30）处结合。 在另一个实施例中，液滴（55）分段成较小的液滴，桥接入口和出口之间的间隙。

公开(公告)号：US08298833B2

公开(公告)日：2012-10-30

申请号：US12092261

申请日：2007-02-07

申请人： Mark Davies ,  Tara Dalton ,  Kieran Curran

发明人： Mark Davies ,  Tara Dalton ,  Kieran Curran

IPC分类号： G01N1/10

CPC分类号： B01L3/502784 ,  B01F13/0071 ,  B01F15/0201 ,  B01F15/0232 ,  B01F2215/0037 ,  B01L3/5025 ,  B01L3/502715 ,  B01L3/502746 ,  B01L7/525 ,  B01L2200/0673 ,  B01L2300/0829 ,  B01L2300/0838 ,  B01L2400/0478 ,  B01L2400/0487 ,  B01L2400/0633 ,  B01L2400/082 ,  C12Q1/686 ,  F17D1/08 ,  Y10T137/87676 ,  Y10T436/117497 ,  Y10T436/118339 ,  Y10T436/2575

摘要： A bridge (30) comprises a first inlet port (31) at the end of a capillary, a narrower second inlet port (32) which is an end of a capillary, an outlet port (33) which is an end of a capillary, and a chamber (34) for silicone oil. The oil is density-matched with the reactor droplets such that a neutrally buoyant environment is created within the chamber (34). The oil within the chamber is continuously replenished by the oil separating the reactor droplets. This causes the droplets to assume a stable capillary-suspended spherical form upon entering the chamber (34). The spherical shape grows until large enough to span the gap between the ports, forming an axisymmetric liquid bridge. The introduction of a second droplet from the second inlet port (32) causes the formation of an unstable funicular bridge that quickly ruptures from the, finer, second inlet port (32), and the droplets combine at the liquid bridge (30). In another embodiment, a droplet (55) segments into smaller droplets which bridge the gap between the inlet and outlet ports.

摘要翻译： 桥（30）包括在毛细管端部的第一入口（31），作为毛细管端部的较窄的第二入口（32），作为毛细管的端部的出口（33） 和用于硅油的室（34）。 油与反应器液滴密度匹配，使得在室（34）内产生中性浮力的环境。 室内的油通过分离反应器液滴的油不断补充。 这使得液滴在进入室（34）时呈现稳定的毛细管悬浮球形。 球形增长直到足够大以跨越端口之间的间隙，形成轴对称液桥。 从第二入口端口（32）引入第二液滴导致形成不稳定的索道桥，其从较细的第二入口（32）快速破裂，并且液滴在液体桥（30）处结合。 在另一个实施例中，液滴（55）分段成较小的液滴，桥接入口和出口之间的间隙。

公开(公告)号：US09631230B2

公开(公告)日：2017-04-25

申请号：US12539343

申请日：2009-08-11

申请人： Mark Davies ,  Tara Dalton

发明人： Mark Davies ,  Tara Dalton

IPC分类号： C12Q1/68 ,  B01L3/00 ,  B01L7/00

CPC分类号： C12Q1/6851 ,  B01L3/502784 ,  B01L7/525 ,  B01L2300/0838 ,  B01L2300/0867 ,  B01L2400/0487 ,  C12Q2561/101 ,  C12Q2563/159 ,  C12Q2565/629

摘要： The invention provides methods of conducting a nucleic acid reaction, including methods for performing digital PCR using a “droplet-in-oil” technology. In the methods, the starting sampled is segmented at least partially into a set of sample droplets each containing on average about one or fewer copies of a target nucleic acid. The droplets are passed in a continuous flow of immiscible carrier fluid through a channel that passes through a thermal cycler, whereby the target is amplified. In one implementation, the droplets are about 350 nl each and the number of positively amplified droplets is counted at the near-saturation point.