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公开(公告)号：US20100068764A1
公开(公告)日：2010-03-18
申请号：US12524495
申请日：2008-02-11
申请人： Ramakrishna Sista , Vamsee K. Pamula , Michael G. Pollack , Vijay Srinivasan , Allen E. Eckhardt
发明人： Ramakrishna Sista , Vamsee K. Pamula , Michael G. Pollack , Vijay Srinivasan , Allen E. Eckhardt
CPC分类号： G01N33/54326 , B01L3/502715 , B01L3/50273 , B01L3/502761 , B01L3/502784 , B01L2200/025 , B01L2200/028 , B01L2200/0647 , B01L2200/0673 , B01L2200/141 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2400/0427 , B01L2400/043 , C12M25/01 , C12M25/16 , G01N27/44717 , G01N27/44786 , G01N33/54386 , G01N35/0098 , G01N2035/1046
摘要： A method of providing a droplet in contact with a magnetically responsive bead and having a reduced quantity of a substance. The method generally includes the steps of (a) providing a droplet actuator (200) comprising: (i) a substrate (210) comprising electrodes (214) arranged for conducting droplet operations on a surface; (ii) a starting droplet (218/222) comprising: (1) one or more magnetically responsive beads (220); (2) a starting quantity of the substance; and (3) a starting volume; (b) magnetically immobilizing the one or more magnetically responsive beads at a location which is at a distance from a target droplet splitting zone, (224); (c) conducting one or more droplet operations comprising droplet dividing operations selected to divide the combined droplet to yield a set of droplets comprising: (i) a droplet (218) comprising substantially all of the one or more magnetically responsive beads and having a decreased quantity of the substance relative to the starting concentration; and (ii) a droplet (222) substantially lacking the magnetically responsive beads.
摘要翻译： 提供与磁响应珠接触并具有减少的物质量的液滴的方法。 该方法通常包括以下步骤：（a）提供液滴致动器（200），包括：（i）包括布置用于在表面上进行液滴操作的电极（214）的基底（210） （ii）起始液滴（218/222），包括：（1）一个或多个磁响应珠（220）; （2）物质的起始量; 和（3）起始体积; （b）将所述一个或多个磁响应珠磁性地固定在距离目标液滴分离区一段距离的位置处（224）; （c）进行一个或多个液滴操作，其包括液滴分割操作，所述液滴分割操作被选择以分割组合的液滴以产生一组液滴，其包括：（i）液滴（218），其基本上包含所有一个或多个磁响应珠粒， 物质的数量相对于起始浓度; 和（ii）基本上不含磁响应珠的液滴（222）。
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公开(公告)号：US20110256542A9
公开(公告)日：2011-10-20
申请号：US12546115
申请日：2009-08-24
申请人： Michael G. Pollack , Zhishan Hua , Allen E. Eckhardt , Prasanna Thwar , Vijay Srinivasan , Vamsee K. Pamula
发明人： Michael G. Pollack , Zhishan Hua , Allen E. Eckhardt , Prasanna Thwar , Vijay Srinivasan , Vamsee K. Pamula
IPC分类号： C12Q1/68
CPC分类号： B01L3/502792 , B01L3/502753 , B01L3/502761 , B01L7/525 , B01L2200/10 , B01L2300/0816 , B01L2300/089 , B01L2400/0427 , B01L2400/043 , C12Q1/6848 , C12Q2565/629
摘要： The invention relates to certain novel approaches to reducing or eliminating the movement of contaminants from one droplet to another on a droplet actuator via liquid filler fluid. In one application, droplet actuators are used to conduct genetic analysis using polymerase chain reaction (PCR) techniques. The invention addresses the need for improved methods of performing PCR on a droplet actuator that provide for optimum amplification and detection of a sample target.
摘要翻译： 本发明涉及在液滴致动器上通过液体填充液减少或消除污染物从一个液滴到另一个液滴的移动的某些新颖方法。 在一个应用中，液滴致动器用于使用聚合酶链反应（PCR）技术进行遗传分析。 本发明解决了对提供样品靶的最佳放大和检测的在液滴致动器上进行PCR的改进方法的需要。
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公开(公告)号：US20100140093A1
公开(公告)日：2010-06-10
申请号：US12615609
申请日：2009-11-10
IPC分类号： C25B7/00
CPC分类号： G01N27/26 , B01F13/0071 , B01F13/0076 , B01L3/0268 , B01L3/502715 , B01L3/502723 , B01L3/502761 , B01L3/502784 , B01L3/502792 , B01L7/52 , B01L7/525 , B01L2200/027 , B01L2200/0668 , B01L2200/0673 , B01L2200/0684 , B01L2300/0654 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2300/1827 , B01L2400/0406 , B01L2400/0415 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0436 , B01L2400/0442 , B01L2400/0448 , C12Q1/686 , C12Q1/6869 , G01N27/3271 , G01N27/416 , G01N27/447 , G01N35/00029 , G01N2035/00158 , G01N2035/00237
摘要： The present invention relates to droplet-based surface modification and washing. According to one embodiment, a method of providing a bead-containing droplet with a reduced concentration of a substance is provided, the method including providing a droplet microactuator including a droplet having a starting volume and including one or more beads and a starting concentration and starting quantity of the substance. The method further includes conducting one or more droplet operations to merge a wash droplet with the droplet provided in the above step to yield a combined droplet and including one or more droplet operations to divide the combined droplet to yield a set of droplets including a droplet including substantially all of the one or more beads and having a decreased concentration of the substance relative to the starting concentration and a droplet which is substantially lacking in the beads.
摘要翻译： 本发明涉及液滴表面改性和洗涤。 根据一个实施例，提供了一种提供具有降低的物质浓度的含珠粒的液滴的方法，所述方法包括提供包括具有起始体积并包括一个或多个珠粒和起始浓度的液滴的液滴微致动器，并起始浓度 物质的数量。 该方法还包括进行一个或多个液滴操作以将洗涤液滴与上述步骤中提供的液滴合并以产生组合液滴并且包括一个或多个液滴操作以分开组合的液滴以产生包括液滴的一组液滴，包括液滴，包括 基本上所有的一种或多种珠粒，并且物质相对于起始浓度降低，而珠子中基本上不含有液滴。
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公开(公告)号：US20090311713A1
公开(公告)日：2009-12-17
申请号：US12546253
申请日：2009-08-24
申请人： Michael G. Pollack , Zhishan Hua , Allen E. Eckhardt , Prasanna Thwar , Vijay Srinivasan , Vamsee K. Pamula
发明人： Michael G. Pollack , Zhishan Hua , Allen E. Eckhardt , Prasanna Thwar , Vijay Srinivasan , Vamsee K. Pamula
IPC分类号： C12Q1/68
CPC分类号： B01L3/502792 , B01L3/502753 , B01L3/502761 , B01L7/525 , B01L2200/10 , B01L2300/0816 , B01L2300/089 , B01L2400/0427 , B01L2400/043 , C12Q1/6848 , C12Q2565/629
摘要： The invention relates to certain novel approaches to reducing or eliminating the movement of contaminants from one droplet to another on a droplet actuator via liquid filler fluid. In one application, a method of detecting an analyte is provided and includes providing in a detection window a droplet including a signal-producing substance indicative of the presence and/or quantity of an analyte and one or more magnetically responsive beads which may interfere with signal produced by the signal producing substance. The method further includes using a magnetic field for magnetically removing the magnetically responsive beads from the detection window and/or magnetically restricting the magnetically responsive beads from entering the detection window while transporting and/or retaining the droplet in the detection window. The method additionally includes detecting a signal produced by the signal-producing substance.
摘要翻译： 本发明涉及在液滴致动器上通过液体填充液减少或消除污染物从一个液滴到另一个液滴的移动的某些新颖方法。 在一个应用中，提供检测分析物的方法，并且包括在检测窗口中提供包含指示分析物的存在和/或量的信号产生物质的液滴和一个或多个可能干扰信号的磁响应珠子 由信号产生物质产生。 该方法还包括使用磁场来从检测窗口磁性地去除磁响应珠子和/或磁力限制磁响应珠子进入检测窗口，同时在检测窗口中传送和/或保持液滴。 该方法还包括检测由信号产生物质产生的信号。
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公开(公告)号：US20110097763A1
公开(公告)日：2011-04-28
申请号：US12991966
申请日：2009-05-13
申请人： Michael G. Pollack , Zhishan Hua , Allen E. Eckhardt , Prasanna Thwar , Vijay Srinivasan , Vamsee K. Pamula
发明人： Michael G. Pollack , Zhishan Hua , Allen E. Eckhardt , Prasanna Thwar , Vijay Srinivasan , Vamsee K. Pamula
IPC分类号： C12P19/34
CPC分类号： B01L7/525 , B01L3/502761 , B01L3/502792 , B01L7/52 , B01L2200/027 , B01L2200/0668 , B01L2200/10 , B01L2300/0803 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/088 , B01L2300/0883 , B01L2300/089 , B01L2300/1827 , B01L2400/0427 , B01L2400/043 , C12Q1/686 , C12Q2565/629
摘要： The invention relates to certain novel approaches to reducing or eliminating the movement of contaminants from one droplet to another on a droplet actuator via liquid filler fluid. In one application, droplet actuators are used to conduct genetic analysis using polymerase chain reaction (PCR) techniques. The invention addresses the need for improved methods of performing PCR on a droplet actuator that provide for optimum amplification and detection of a sample target.
摘要翻译： 本发明涉及在液滴致动器上通过液体填充液减少或消除污染物从一个液滴到另一个液滴的移动的某些新颖方法。 在一个应用中，液滴致动器用于使用聚合酶链反应（PCR）技术进行遗传分析。 本发明解决了对提供样品靶的最佳放大和检测的在液滴致动器上进行PCR的改进方法的需要。
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公开(公告)号：US20070243634A1
公开(公告)日：2007-10-18
申请号：US11639736
申请日：2006-12-15
IPC分类号： G01N33/543
CPC分类号： G01N27/26 , B01F13/0071 , B01F13/0076 , B01L3/0268 , B01L3/502715 , B01L3/502723 , B01L3/502761 , B01L3/502784 , B01L3/502792 , B01L7/52 , B01L7/525 , B01L2200/027 , B01L2200/0668 , B01L2200/0673 , B01L2200/0684 , B01L2300/0654 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2300/1827 , B01L2400/0406 , B01L2400/0415 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0436 , B01L2400/0442 , B01L2400/0448 , C12Q1/686 , C12Q1/6869 , G01N27/3271 , G01N27/416 , G01N27/447 , G01N35/00029 , G01N2035/00158 , G01N2035/00237
摘要： The present invention relates to droplet-based surface modification and washing. According to one embodiment, a method of providing a droplet in contact with a surface with a reduced concentration of a substance is provided, wherein the method includes: (a) providing a droplet microactuator comprising a surface in contact with a droplet comprising a starting concentration and starting quantity of the substance and having a starting volume; (b) conducting one or more droplet operations to merge a wash droplet with the droplet provided in step (a) to yield a combined droplet; and (c) conducting one or more droplet operations to divide the combined droplet to yield a set of droplets comprising: (i) a droplet in contact with the surface having a decreased concentration of the substance relative to the starting concentration; and (ii) a droplet which is separated from the surface.
摘要翻译： 本发明涉及液滴表面改性和洗涤。 根据一个实施例，提供了一种提供与物质浓度降低的表面接触的液滴的方法，其中所述方法包括：（a）提供液滴微致动器，其包括与包含起始浓度的液滴的表面 并开始物质的起始体积; （b）进行一个或多个液滴操作以将洗涤液滴与步骤（a）中提供的液滴合并以产生组合液滴; 和（c）进行一个或多个液滴操作以分开组合的液滴以产生一组液滴，其包括：（i）与所述表面接触的液滴相对于起始浓度具有降低的物质浓度; 和（ii）与表面分离的液滴。
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公开(公告)号：US08562807B2
公开(公告)日：2013-10-22
申请号：US12747231
申请日：2008-12-10
申请人： Vijay Srinivasan , Michael G. Pollack , Zhishan Hua , Arjun Sudarsan , Allen E. Eckhardt , Vamsee K. Pamula
发明人： Vijay Srinivasan , Michael G. Pollack , Zhishan Hua , Arjun Sudarsan , Allen E. Eckhardt , Vamsee K. Pamula
IPC分类号： G01N27/453
CPC分类号： G01N27/44743 , B01L3/0268 , B01L3/502761 , B01L3/502792 , B01L2200/0605 , B01L2200/061 , B01L2200/0647 , B01L2300/0645 , B01L2300/16 , B01L2400/0415 , B01L2400/0427
摘要： Droplet actuators for conducting droplet operations, such as droplet transport and droplet dispensing, are provided. In one embodiment, the droplet actuator may include a substrate including, droplet operations electrodes arranged for conducting droplet operations on a surface of the substrate; and reference electrodes associated with the droplet operations electrodes and extending above the surface of the substrate. Other embodiments of droplet actuators and methods of loading and using such droplet actuators are also provided.
摘要翻译： 提供用于进行液滴操作的滴液致动器，例如液滴传输和液滴分配。 在一个实施例中，液滴致动器可以包括基板，其包括布置成在基板的表面上进行液滴操作的液滴操作电极; 以及与液滴操作电极相关并在衬底表面上方延伸的参考电极。 还提供了液滴致动器的其他实施例以及加载和使用这种液滴致动器的方法。
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公开(公告)号：US07815871B2
公开(公告)日：2010-10-19
申请号：US11639664
申请日：2006-12-15
CPC分类号： B41J2/125 , B01L3/502792 , B01L2200/143 , B01L2200/147 , B01L2300/0645 , B01L2300/0654 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0487 , B41J2/17509 , G01N15/1484 , G01N2015/1006
摘要： The present invention relates to a droplet microactuator system. According to one embodiment, the droplet microactuator system includes: (a) a droplet microactuator configured to conduct droplet operations; (b) a magnetic field source arranged to immobilize magnetically responsive beads in a droplet during droplet operations; (c) a sensor configured in a sensing relationship with the droplet microactuator, such that the sensor is capable of sensing a signal from and/or a property of one or more droplets on the droplet microactuator; and (d) one or more processors electronically coupled to the droplet microactuator and programmed to control electrowetting-mediated droplet operations on the droplet actuator and process electronic signals from the sensor.
摘要翻译： 液滴微致动器系统技术领域本发明涉及液滴微致动器系统。 根据一个实施例，液滴微致动器系统包括：（a）构造成进行液滴操作的液滴微致动器; （b）磁场源，布置成在液滴操作期间将磁响应珠固定在液滴中; （c）配置成与液滴微致动器的感测关系的传感器，使得传感器能够感测来自液滴微致动器上的一个或多个液滴的信号和/或特性; 和（d）电子耦合到液滴微致动器的一个或多个处理器，并被编程为控制液滴致动器上的电润湿介导的液滴操作并处理来自传感器的电子信号。
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公开(公告)号：US20100221713A1
公开(公告)日：2010-09-02
申请号：US12546115
申请日：2009-08-24
申请人： Michael G. Pollack , Zhishan Hua , Allen E. Eckhardt , Prasanna Thwar , Vijay Srinivasan , Vamsee K. Pamula
发明人： Michael G. Pollack , Zhishan Hua , Allen E. Eckhardt , Prasanna Thwar , Vijay Srinivasan , Vamsee K. Pamula
IPC分类号： C12Q1/68
CPC分类号： B01L3/502792 , B01L3/502753 , B01L3/502761 , B01L7/525 , B01L2200/10 , B01L2300/0816 , B01L2300/089 , B01L2400/0427 , B01L2400/043 , C12Q1/6848 , C12Q2565/629
摘要： The invention relates to certain novel approaches to reducing or eliminating the movement of contaminants from one droplet to another on a droplet actuator via liquid filler fluid. In one application, droplet actuators are used to conduct genetic analysis using polymerase chain reaction (PCR) techniques. The invention addresses the need for improved methods of performing PCR on a droplet actuator that provide for optimum amplification and detection of a sample target.
摘要翻译： 本发明涉及在液滴致动器上通过液体填充液减少或消除污染物从一个液滴到另一个液滴的移动的某些新颖方法。 在一个应用中，液滴致动器用于使用聚合酶链反应（PCR）技术进行遗传分析。 本发明解决了对提供样品靶的最佳放大和检测的在液滴致动器上进行PCR的改进方法的需要。
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公开(公告)号：US07439014B2
公开(公告)日：2008-10-21
申请号：US11639736
申请日：2006-12-15
IPC分类号： C12Q1/00
CPC分类号： G01N27/26 , B01F13/0071 , B01F13/0076 , B01L3/0268 , B01L3/502715 , B01L3/502723 , B01L3/502761 , B01L3/502784 , B01L3/502792 , B01L7/52 , B01L7/525 , B01L2200/027 , B01L2200/0668 , B01L2200/0673 , B01L2200/0684 , B01L2300/0654 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2300/1827 , B01L2400/0406 , B01L2400/0415 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0436 , B01L2400/0442 , B01L2400/0448 , C12Q1/686 , C12Q1/6869 , G01N27/3271 , G01N27/416 , G01N27/447 , G01N35/00029 , G01N2035/00158 , G01N2035/00237
摘要： The present invention relates to droplet-based surface modification and washing. According to one embodiment, a method of providing a droplet in contact with a surface with a reduced concentration of a substance is provided, wherein the method includes: (a) providing a droplet microactuator comprising a surface in contact with a droplet comprising a starting concentration and starting quantity of the substance and having a starting volume; (b) conducting one or more droplet operations to merge a wash droplet with the droplet provided in step (a) to yield a combined droplet; and (c) conducting one or more droplet operations to divide the combined droplet to yield a set of droplets comprising: (i) a droplet in contact with the surface having a decreased concentration of the substance relative to the starting concentration; and (ii) a droplet which is separated from the surface.
摘要翻译： 本发明涉及液滴表面改性和洗涤。 根据一个实施例，提供了一种提供与物质浓度降低的表面接触的液滴的方法，其中所述方法包括：（a）提供液滴微致动器，其包括与包含起始浓度的液滴的表面 并开始物质的起始体积; （b）进行一个或多个液滴操作以将洗涤液滴与步骤（a）中提供的液滴合并以产生组合液滴; 和（c）进行一个或多个液滴操作以分开组合的液滴以产生一组液滴，其包括：（i）与所述表面接触的液滴相对于起始浓度具有降低的物质浓度; 和（ii）与表面分离的液滴。
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