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公开(公告)号：US20100140093A1
公开(公告)日：2010-06-10
申请号：US12615609
申请日：2009-11-10
IPC分类号： C25B7/00
CPC分类号： G01N27/26 , B01F13/0071 , B01F13/0076 , B01L3/0268 , B01L3/502715 , B01L3/502723 , B01L3/502761 , B01L3/502784 , B01L3/502792 , B01L7/52 , B01L7/525 , B01L2200/027 , B01L2200/0668 , B01L2200/0673 , B01L2200/0684 , B01L2300/0654 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2300/1827 , B01L2400/0406 , B01L2400/0415 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0436 , B01L2400/0442 , B01L2400/0448 , C12Q1/686 , C12Q1/6869 , G01N27/3271 , G01N27/416 , G01N27/447 , G01N35/00029 , G01N2035/00158 , G01N2035/00237
摘要： The present invention relates to droplet-based surface modification and washing. According to one embodiment, a method of providing a bead-containing droplet with a reduced concentration of a substance is provided, the method including providing a droplet microactuator including a droplet having a starting volume and including one or more beads and a starting concentration and starting quantity of the substance. The method further includes conducting one or more droplet operations to merge a wash droplet with the droplet provided in the above step to yield a combined droplet and including one or more droplet operations to divide the combined droplet to yield a set of droplets including a droplet including substantially all of the one or more beads and having a decreased concentration of the substance relative to the starting concentration and a droplet which is substantially lacking in the beads.
摘要翻译： 本发明涉及液滴表面改性和洗涤。 根据一个实施例，提供了一种提供具有降低的物质浓度的含珠粒的液滴的方法，所述方法包括提供包括具有起始体积并包括一个或多个珠粒和起始浓度的液滴的液滴微致动器，并起始浓度 物质的数量。 该方法还包括进行一个或多个液滴操作以将洗涤液滴与上述步骤中提供的液滴合并以产生组合液滴并且包括一个或多个液滴操作以分开组合的液滴以产生包括液滴的一组液滴，包括液滴，包括 基本上所有的一种或多种珠粒，并且物质相对于起始浓度降低，而珠子中基本上不含有液滴。
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公开(公告)号：US20070243634A1
公开(公告)日：2007-10-18
申请号：US11639736
申请日：2006-12-15
IPC分类号： G01N33/543
CPC分类号： G01N27/26 , B01F13/0071 , B01F13/0076 , B01L3/0268 , B01L3/502715 , B01L3/502723 , B01L3/502761 , B01L3/502784 , B01L3/502792 , B01L7/52 , B01L7/525 , B01L2200/027 , B01L2200/0668 , B01L2200/0673 , B01L2200/0684 , B01L2300/0654 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2300/1827 , B01L2400/0406 , B01L2400/0415 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0436 , B01L2400/0442 , B01L2400/0448 , C12Q1/686 , C12Q1/6869 , G01N27/3271 , G01N27/416 , G01N27/447 , G01N35/00029 , G01N2035/00158 , G01N2035/00237
摘要： The present invention relates to droplet-based surface modification and washing. According to one embodiment, a method of providing a droplet in contact with a surface with a reduced concentration of a substance is provided, wherein the method includes: (a) providing a droplet microactuator comprising a surface in contact with a droplet comprising a starting concentration and starting quantity of the substance and having a starting volume; (b) conducting one or more droplet operations to merge a wash droplet with the droplet provided in step (a) to yield a combined droplet; and (c) conducting one or more droplet operations to divide the combined droplet to yield a set of droplets comprising: (i) a droplet in contact with the surface having a decreased concentration of the substance relative to the starting concentration; and (ii) a droplet which is separated from the surface.
摘要翻译： 本发明涉及液滴表面改性和洗涤。 根据一个实施例，提供了一种提供与物质浓度降低的表面接触的液滴的方法，其中所述方法包括：（a）提供液滴微致动器，其包括与包含起始浓度的液滴的表面 并开始物质的起始体积; （b）进行一个或多个液滴操作以将洗涤液滴与步骤（a）中提供的液滴合并以产生组合液滴; 和（c）进行一个或多个液滴操作以分开组合的液滴以产生一组液滴，其包括：（i）与所述表面接触的液滴相对于起始浓度具有降低的物质浓度; 和（ii）与表面分离的液滴。
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公开(公告)号：US07439014B2
公开(公告)日：2008-10-21
申请号：US11639736
申请日：2006-12-15
IPC分类号： C12Q1/00
CPC分类号： G01N27/26 , B01F13/0071 , B01F13/0076 , B01L3/0268 , B01L3/502715 , B01L3/502723 , B01L3/502761 , B01L3/502784 , B01L3/502792 , B01L7/52 , B01L7/525 , B01L2200/027 , B01L2200/0668 , B01L2200/0673 , B01L2200/0684 , B01L2300/0654 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2300/1827 , B01L2400/0406 , B01L2400/0415 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0436 , B01L2400/0442 , B01L2400/0448 , C12Q1/686 , C12Q1/6869 , G01N27/3271 , G01N27/416 , G01N27/447 , G01N35/00029 , G01N2035/00158 , G01N2035/00237
摘要： The present invention relates to droplet-based surface modification and washing. According to one embodiment, a method of providing a droplet in contact with a surface with a reduced concentration of a substance is provided, wherein the method includes: (a) providing a droplet microactuator comprising a surface in contact with a droplet comprising a starting concentration and starting quantity of the substance and having a starting volume; (b) conducting one or more droplet operations to merge a wash droplet with the droplet provided in step (a) to yield a combined droplet; and (c) conducting one or more droplet operations to divide the combined droplet to yield a set of droplets comprising: (i) a droplet in contact with the surface having a decreased concentration of the substance relative to the starting concentration; and (ii) a droplet which is separated from the surface.
摘要翻译： 本发明涉及液滴表面改性和洗涤。 根据一个实施例，提供了一种提供与物质浓度降低的表面接触的液滴的方法，其中所述方法包括：（a）提供液滴微致动器，其包括与包含起始浓度的液滴的表面 并开始物质的起始体积; （b）进行一个或多个液滴操作以将洗涤液滴与步骤（a）中提供的液滴合并以产生组合液滴; 和（c）进行一个或多个液滴操作以分开组合的液滴以产生一组液滴，其包括：（i）与所述表面接触的液滴相对于起始浓度具有降低的物质浓度; 和（ii）与表面分离的液滴。
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公开(公告)号：US20070241068A1
公开(公告)日：2007-10-18
申请号：US11639531
申请日：2006-12-15
IPC分类号： B01D57/00
CPC分类号： B01L3/502784 , B01F11/0071 , B01F13/0071 , B01F13/0076 , B01L3/502715 , B01L3/502761 , B01L3/502792 , B01L7/52 , B01L7/525 , B01L2200/027 , B01L2300/0654 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2300/1827 , B01L2400/0406 , B01L2400/0415 , B01L2400/0421 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0436 , B01L2400/0442 , Y10T436/11 , Y10T436/25 , Y10T436/25625 , Y10T436/2575
摘要： The present invention relates to droplet-based washing. According to one embodiment, a method of providing a droplet in contact with a surface with a reduced concentration of a substance is provided, wherein the method includes: (a) providing a surface in contact with a droplet comprising a starting concentration and starting quantity of the substance and having a starting volume; (b) conducting one or more droplet operations to merge a wash droplet with the droplet provided in step (a) to yield a combined droplet; and (c) conducting one or more droplet operations to divide the combined droplet to yield a set of droplets comprising: (i) a droplet in contact with the surface having a decreased concentration of the substance relative to the starting concentration; and (ii) a droplet which is separated from the surface.
摘要翻译： 本发明涉及液滴洗涤。 根据一个实施方案，提供了一种提供与具有降低的物质浓度的表面接触的液滴的方法，其中所述方法包括：（a）提供与包含起始浓度和起始浓度的液滴接触的表面 该物质并具有起始体积; （b）进行一个或多个液滴操作以将洗涤液滴与步骤（a）中提供的液滴合并以产生组合液滴; 和（c）进行一个或多个液滴操作以分开组合的液滴以产生一组液滴，其包括：（i）与所述表面接触的液滴相对于起始浓度具有降低的物质浓度; 和（ii）与表面分离的液滴。
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公开(公告)号：US09046514B2
公开(公告)日：2015-06-02
申请号：US12524495
申请日：2008-02-11
申请人： Ramakrishna Sista , Vamsee K. Pamula , Michael G. Pollack , Vijay Srinivasan , Allen E. Eckhardt , Richard B. Fair
发明人： Ramakrishna Sista , Vamsee K. Pamula , Michael G. Pollack , Vijay Srinivasan , Allen E. Eckhardt , Richard B. Fair
CPC分类号： G01N33/54326 , B01L3/502715 , B01L3/50273 , B01L3/502761 , B01L3/502784 , B01L2200/025 , B01L2200/028 , B01L2200/0647 , B01L2200/0673 , B01L2200/141 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2400/0427 , B01L2400/043 , C12M25/01 , C12M25/16 , G01N27/44717 , G01N27/44786 , G01N33/54386 , G01N35/0098 , G01N2035/1046
摘要： A method of providing a droplet in contact with a magnetically responsive bead and having a reduced quantity of a substance. The method generally includes the steps of (a) providing a droplet actuator (200) comprising: (i) a substrate (210) comprising electrodes (214) arranged for conducting droplet operations on a surface; (ii) a starting droplet (218/222) comprising: (1) one or more magnetically responsive beads (220); (2) a starting quantity of the substance; and (3) a starting volume; (b) magnetically immobilizing the one or more magnetically responsive beads at a location which is at a distance from a target droplet splitting zone, (224); (c) conducting one or more droplet operations comprising droplet dividing operations selected to divide the combined droplet to yield a set of droplets comprising: (i) a droplet (218) comprising substantially all of the one or more magnetically responsive beads and having a decreased quantity of the substance relative to the starting concentration; and (ii) a droplet (222) substantially lacking the magnetically responsive beads.
摘要翻译： 提供与磁响应珠接触并具有减少的物质量的液滴的方法。 该方法通常包括以下步骤：（a）提供液滴致动器（200），包括：（i）包括布置用于在表面上进行液滴操作的电极（214）的基底（210） （ii）起始液滴（218/222），包括：（1）一个或多个磁响应珠（220）; （2）物质的起始量; 和（3）起始体积; （b）将所述一个或多个磁响应珠磁性地固定在距离目标液滴分离区一段距离的位置处（224）; （c）进行一个或多个液滴操作，其包括液滴分割操作，所述液滴分割操作被选择以分割组合的液滴以产生一组液滴，其包括：（i）液滴（218），其基本上包含所有一个或多个磁响应珠粒， 物质的数量相对于起始浓度; 和（ii）基本上不含磁响应珠的液滴（222）。
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公开(公告)号：US08846414B2
公开(公告)日：2014-09-30
申请号：US12569725
申请日：2009-09-29
申请人： Ramakrishna Sista , Vamsee K. Pamula , Arjun Sudarsan , Vijay Srinivasan , Michael G. Pollack , Richard B. Fair , Allen E. Eckhardt
发明人： Ramakrishna Sista , Vamsee K. Pamula , Arjun Sudarsan , Vijay Srinivasan , Michael G. Pollack , Richard B. Fair , Allen E. Eckhardt
IPC分类号： G01N33/553 , G01N33/68 , G01N33/543
CPC分类号： G01N33/6893 , G01N33/54366 , G01N2333/4712 , G01N2333/58 , G01N2800/32 , Y10T436/25
摘要： The present invention provides for the detection of cardiac markers on a droplet actuator. An aspect provides a method of assaying a cardiac marker in a biological sample from a subject, the method including providing a droplet actuator, loading the biological sample and assay reagents on the droplet actuator, executing droplet operations to create sample droplets from the sample and reagent droplets from the reagents on the droplet actuator, and executing droplet operations using the sample droplets and reagent droplets to produce a detectable signal indicative of the quantity of the cardiac marker in the biological sample. Still other aspects are provided.
摘要翻译： 本发明提供了在液滴致动器上检测心脏标志物。 一方面提供了从受试者的生物样品中测定心脏标志物的方法，所述方法包括提供液滴致动器，在液滴致动器上装载生物样品和测定试剂，执行液滴操作以从样品和试剂产生样品液滴 来自液滴致动器上的试剂的液滴，以及使用样品液滴和试剂液滴执行液滴操作，以产生指示生物样品中心脏标志物量的可检测信号。 还提供了其他方面。
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公开(公告)号：US08541176B2
公开(公告)日：2013-09-24
申请号：US12113385
申请日：2008-05-01
IPC分类号： G01N33/53
CPC分类号： G01N27/26 , B01F13/0071 , B01F13/0076 , B01L3/0268 , B01L3/502715 , B01L3/502723 , B01L3/502761 , B01L3/502784 , B01L3/502792 , B01L7/52 , B01L7/525 , B01L2200/027 , B01L2200/0668 , B01L2200/0673 , B01L2200/0684 , B01L2300/0654 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2300/1827 , B01L2400/0406 , B01L2400/0415 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0436 , B01L2400/0442 , B01L2400/0448 , C12Q1/686 , C12Q1/6869 , G01N27/3271 , G01N27/416 , G01N27/447 , G01N35/00029 , G01N2035/00158 , G01N2035/00237
摘要： The present invention relates to droplet-based surface modification and washing. According to one embodiment, a method of modifying a surface on a droplet microactuator is provided, wherein the method includes executing one or more droplet operations to bring a droplet comprising a surface-modifying agent into contact with the surface. According to another embodiment, a droplet microactuator is provided and includes a sample or reagent immobilized on a surface thereof and arranged such that a droplet on the droplet microactuator may contact the surface. According to yet another embodiment, a method of removing a substance from a surface to which the substance is bound is provided, the method including conducting one or more droplet operations to contact a droplet with the surface, the droplet comprising a solution for eluting the substance from the surface
摘要翻译： 本发明涉及液滴表面改性和洗涤。 根据一个实施例，提供了修改液滴微致动器上的表面的方法，其中所述方法包括执行一个或多个液滴操作以使包含表面改性剂的液滴与表面接触。 根据另一个实施例，提供液滴微致动器，并且包括固定在其表面上的样品或试剂，并且布置成使得液滴微致动器上的液滴可接触表面。 根据另一个实施方案，提供从物质结合的表面除去物质的方法，所述方法包括进行一个或多个液滴操作以使液滴与表面接触，所述液滴包含用于洗脱物质的溶液 从表面
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公开(公告)号：US08313895B2
公开(公告)日：2012-11-20
申请号：US12615609
申请日：2009-11-10
IPC分类号： C12Q1/00
CPC分类号： G01N27/26 , B01F13/0071 , B01F13/0076 , B01L3/0268 , B01L3/502715 , B01L3/502723 , B01L3/502761 , B01L3/502784 , B01L3/502792 , B01L7/52 , B01L7/525 , B01L2200/027 , B01L2200/0668 , B01L2200/0673 , B01L2200/0684 , B01L2300/0654 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2300/1827 , B01L2400/0406 , B01L2400/0415 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0436 , B01L2400/0442 , B01L2400/0448 , C12Q1/686 , C12Q1/6869 , G01N27/3271 , G01N27/416 , G01N27/447 , G01N35/00029 , G01N2035/00158 , G01N2035/00237
摘要： The present invention relates to droplet-based surface modification and washing. According to one embodiment, a method of providing a bead-containing droplet with a reduced concentration of a substance is provided, the method including providing a droplet microactuator including a droplet having a starting volume and including one or more beads and a starting concentration and starting quantity of the substance. The method further includes conducting one or more droplet operations to merge a wash droplet with the droplet provided in the above step to yield a combined droplet and including one or more droplet operations to divide the combined droplet to yield a set of droplets including a droplet including substantially all of the one or more beads and having a decreased concentration of the substance relative to the starting concentration and a droplet which is substantially lacking in the beads.
摘要翻译： 本发明涉及液滴表面改性和洗涤。 根据一个实施例，提供了一种提供具有降低的物质浓度的含珠粒的液滴的方法，所述方法包括提供包括具有起始体积并包括一个或多个珠粒和起始浓度的液滴的液滴微致动器，并起始浓度 物质的数量。 该方法还包括进行一个或多个液滴操作以将洗涤液滴与上述步骤中提供的液滴合并以产生组合液滴并且包括一个或多个液滴操作以分开组合的液滴以产生包括液滴的一组液滴，包括液滴，包括 基本上所有的一种或多种珠粒，并且物质相对于起始浓度降低，而珠子中基本上不含有液滴。
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公开(公告)号：US20120165238A1
公开(公告)日：2012-06-28
申请号：US12113385
申请日：2008-05-01
CPC分类号： G01N27/26 , B01F13/0071 , B01F13/0076 , B01L3/0268 , B01L3/502715 , B01L3/502723 , B01L3/502761 , B01L3/502784 , B01L3/502792 , B01L7/52 , B01L7/525 , B01L2200/027 , B01L2200/0668 , B01L2200/0673 , B01L2200/0684 , B01L2300/0654 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2300/1827 , B01L2400/0406 , B01L2400/0415 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0436 , B01L2400/0442 , B01L2400/0448 , C12Q1/686 , C12Q1/6869 , G01N27/3271 , G01N27/416 , G01N27/447 , G01N35/00029 , G01N2035/00158 , G01N2035/00237
摘要： The present invention relates to droplet-based surface modification and washing. According to one embodiment, a method of modifying a surface on a droplet microactuator is provided, wherein the method includes executing one or more droplet operations to bring a droplet comprising a surface-modifying agent into contact with the surface. According to another embodiment, a droplet microactuator is provided and includes a sample or reagent immobilized on a surface thereof and arranged such that a droplet on the droplet microactuator may contact the surface. According to yet another embodiment, a method of removing a substance from a surface to which the substance is bound is provided, the method including conducting one or more droplet operations to contact a droplet with the surface, the droplet comprising a solution for eluting the substance from the surface
摘要翻译： 本发明涉及液滴表面改性和洗涤。 根据一个实施例，提供了修改液滴微致动器上的表面的方法，其中所述方法包括执行一个或多个液滴操作以使包含表面改性剂的液滴与表面接触。 根据另一个实施例，提供液滴微致动器，并且包括固定在其表面上的样品或试剂，并且布置成使得液滴微致动器上的液滴可接触表面。 根据另一个实施方案，提供从物质结合的表面除去物质的方法，所述方法包括进行一个或多个液滴操作以使液滴与表面接触，所述液滴包含用于洗脱物质的溶液 从表面
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公开(公告)号：US20100116640A1
公开(公告)日：2010-05-13
申请号：US12615666
申请日：2009-11-10
IPC分类号： B01D57/02
CPC分类号： G01N27/26 , B01F13/0071 , B01F13/0076 , B01L3/0268 , B01L3/502715 , B01L3/502723 , B01L3/502761 , B01L3/502784 , B01L3/502792 , B01L7/52 , B01L7/525 , B01L2200/027 , B01L2200/0668 , B01L2200/0673 , B01L2200/0684 , B01L2300/0654 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2300/1827 , B01L2400/0406 , B01L2400/0415 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0436 , B01L2400/0442 , B01L2400/0448 , C12Q1/686 , C12Q1/6869 , G01N27/3271 , G01N27/416 , G01N27/447 , G01N35/00029 , G01N2035/00158 , G01N2035/00237
摘要： The present invention relates to droplet-based surface modification and washing. According to one embodiment, a method of splitting a droplet is provided, the method including providing a droplet microactuator including a droplet including one or more beads and immobilizing at least one of the one or more beads. The method further includes conducting one or more droplet operations to divide the droplet to yield a set of droplets including a droplet including the one or more immobilized beads and a droplet substantially lacking the one or more immobilized beads.
摘要翻译： 本发明涉及液滴表面改性和洗涤。 根据一个实施例，提供了一种分离液滴的方法，所述方法包括提供包括包含一个或多个珠并且固定所述一个或多个珠中的至少一个珠的液滴的液滴微致动器。 该方法还包括进行一个或多个液滴操作以分开液滴以产生一组液滴，包括包含一个或多个固定化珠粒的液滴和基本上不含一个或多个固定化珠粒的液滴。
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