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17 条记录 (耗时 0.032 秒)

    Method for the Synthesis of Oligonucleotide Derivatives
    6.
    发明申请
    Method for the Synthesis of Oligonucleotide Derivatives 失效
    寡核苷酸衍生物的合成方法

    公开(公告)号：US20110245478A1

    公开(公告)日：2011-10-06

    申请号：US13081986

    申请日：2011-04-07

    申请人： François Morvan ,  Albert Meyer ,  Jean-Jacques Vasseur ,  Sébastien Vidal ,  Jean-Pierre Cloarec ,  Yann Chevolot ,  Eliane Souteyrand

    发明人： François Morvan ,  Albert Meyer ,  Jean-Jacques Vasseur ,  Sébastien Vidal ,  Jean-Pierre Cloarec ,  Yann Chevolot ,  Eliane Souteyrand

    IPC分类号： C07H21/04

    CPC分类号： C07H21/00 ,  Y10T436/143333

    摘要： Method for the synthesis of nucleotide derivatives wherein molecules of interest are grafted on the oligonucleotide with the help of a “click chemistry” reaction between an azide function on the molecule of interest and an alkyne function on the oligonucleotide, or between an alkyne function on the molecule of interest and an azide function on the oligonucleotide.Intermediate molecules, notably alkyne functionalized oligonucleotides, grafted oligonucleotides, azide functionalized oligonucleotides, oligonucleotide micro arrays containing them and the use of those grafted oligonucleotides for biological investigation and for cell targeting.

    摘要翻译： 合成核苷酸衍生物的方法，其中目的分子在寡核苷酸上接枝，借助于目标分子上的叠氮化物官能团与寡核苷酸上的炔官能团之间的“点击化学”反应，或者在 目标分子和寡核苷酸上的叠氮基功能。 中间分子，特别是炔官能化寡核苷酸，接枝寡核苷酸，叠氮化物官能化寡核苷酸，含有它们的寡核苷酸微阵列以及这些接枝寡核苷酸用于生物学研究和用于细胞靶向的用途。

    Mixing and Dispensing Homogeneous Compounds of a Reactant on a Surface
    9.
    发明申请
    Mixing and Dispensing Homogeneous Compounds of a Reactant on a Surface 失效
    在表面上混合和分配均匀的反应物的化合物

    公开(公告)号：US20080223115A1

    公开(公告)日：2008-09-18

    申请号：US11988640

    申请日：2006-07-12

    申请人： Vincent Dugas ,  Jerome Broutin ,  Eliane Souteyrand

    发明人： Vincent Dugas ,  Jerome Broutin ,  Eliane Souteyrand

    IPC分类号： G01N33/00 ,  B05D3/00 ,  B05C5/00

    CPC分类号： B01L3/502715 ,  B01F11/0071 ,  B01F13/0059 ,  B01J2219/00286 ,  B01J2219/00527 ,  B01L3/0293 ,  B01L3/502723 ,  B01L9/527 ,  B01L2200/027 ,  B01L2300/0636 ,  B01L2300/0877 ,  G01N1/38 ,  Y10T436/25 ,  Y10T436/25375 ,  Y10T436/255 ,  Y10T436/2575

    摘要： A method for mixing and dispensing homogeneous compounds on a surface, at least one reactant in a carrier fluid in laminar flow, and cell (3) for implementing the method. The cell includes reaction chamber (16) having at least one reaction surface (13) where the reactant can be fixed, directly or indirectly, optionally reversibly, at least three fluid inlets/outlets (25, 26), at least one fixed volume reservoir in communication with the reaction chamber and outside the reaction chamber via injection orifice (27), a fluid loop including at least one feeding port, at least one extraction port and at least one reservoir whose volume is variable and adapted to communicate independently with the reaction chamber via each of its inlets/outlets; device for circulating fluids in the reaction chamber and the fluid loop. The method and cell can be used to prepare homogeneous surface films, or to carry out “target-probe” identification reactions.

    摘要翻译： 用于在表面上混合和分配均匀化合物的方法，层流中载体流体中的至少一种反应物和用于实施该方法的细胞（3）。 电池包括具有至少一个反应表面（13）的反应室（16），其中反应物可以直接或间接地可选地可逆地固定至少三个流体入口/出口（25,26），至少一个固定容积的容器 通过注射孔（27）与反应室和反应室外连通，流体回路包括至少一个进料口，至少一个萃取口和至少一个储存器，其容积是可变的并适于与反应物独立地连通 通过其每个入口/出口; 在反应室和流体回路中循环流体的装置。 该方法和细胞可用于制备均匀的表面膜，或进行“靶 - 探针”鉴定反应。

    Method for analyzing biological substances in a conductive liquid medium
    10.
    发明授权
    Method for analyzing biological substances in a conductive liquid medium 失效
    用于分析导电液体介质中的生物物质的方法

    公开(公告)号：US06355436B1

    公开(公告)日：2002-03-12

    申请号：US09660189

    申请日：2000-09-12

    申请人： Jean-Rene Martin ,  Eliane Souteyrand ,  Marcus Francois Lawrence ,  Susan Rosalie Mikkelsen

    发明人： Jean-Rene Martin ,  Eliane Souteyrand ,  Marcus Francois Lawrence ,  Susan Rosalie Mikkelsen

    IPC分类号： C12Q168

    CPC分类号： G01N33/5438

    摘要： The present invention relates to a procedure for the qualitative and/or quantitative analysis of biological substances, which are preferably biological substances, that are present in a conductive liquid medium, with the aid of at least one affinity sensor that includes at least one structure that includes at least one semiconductor material, which is coated on one of its surface with at least one layer of an isolating material, which in turn is affixed adhesively to at least one sensitive membrane, which is in contact with the conductive medium and which includes ligands that are complementary to the biological substances in question and which are capable of, and suitable for, forming pairs specifically with the latter biological substances, with the said procedure being characterized by the fact that it consists essentially of applying a voltage between the semiconductor and the conductive medium; of gathering the variations in the electrical signals induced by a charge-effect phenomenon directly and essentially linked to the specific pairings of the biological substances with their complementary ligands included in the sensitive membrane; and interpreting the collected signals in order to identify and/or quantify the substances in question.

    摘要翻译： 本发明涉及通过至少一种亲和力传感器来定性和/或定量分析存在于导电液体介质中的生物物质的生物物质的方法，所述亲和力传感器包括至少一种结构， 包括至少一种半导体材料，其在其一个表面上涂覆有至少一层隔离材料，所述隔离材料层又粘附于至少一个与导电介质接触的敏感膜并且其包括配体 它们与所讨论的生物物质互补，并且能够并且适合于与后一种生物物质特异性地形成对，所述方法的特征在于其基本上由半导体和 导电介质; 收集由电荷效应现象引起的电信号的变化，直接和基本上与生物物质与敏感膜中包含的互补配体的特定配对相关; 并解释收集的信号，以便识别和/或量化所讨论的物质。