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    Monoclonal antibody recognizing FK506-binding protein, method for assaying FK506-binding protein level, and kit therefor
    5.
    发明授权
    Monoclonal antibody recognizing FK506-binding protein, method for assaying FK506-binding protein level, and kit therefor 失效
    识别FK506结合蛋白的单克隆抗体，用于测定FK506结合蛋白水平的方法及其试剂盒

    公开(公告)号：US5576183A

    公开(公告)日：1996-11-19

    申请号：US379563

    申请日：1995-02-10

    申请人： Masakazu Kobayashi ,  Kazuyuki Ohtsuka ,  Hirokazu Tanaka ,  Mineo Niwa

    发明人： Masakazu Kobayashi ,  Kazuyuki Ohtsuka ,  Hirokazu Tanaka ,  Mineo Niwa

    IPC分类号： C07K16/28 ,  C12P21/08 ,  G01N33/53 ,  C07K16/18

    CPC分类号： C07K16/28

    摘要： The present invention relates to a monoclonal antibody (anti-FKBP antibody) which recognizes an antigenic determinant in an FK506-binding protein and does not inhibit the binding between an FK506-binding protein and FK506, a method for assaying an FKBP level in plasma, comprising reacting an immobilized anti-FKBP antibody, an enzyme-labelled FK506 and FKBP in a specimen, and assaying the degree of color development of an enzyme substrate, and a kit for the assay. According to the present invention, the FKBP level in plasma which affects the immunosuppressive action of FK506 can be assayed more precisely with ease, which in turn enables setting the optimal dose of FK506 in close accordance with the condition of individual patients.

    摘要翻译： PCT No.PCT / JP93 / 01136 Sec。 371日期1995年2月10日 102（e）日期1995年2月10日PCT提交1993年8月11日PCT公布。 第WO94 / 04700号公报 1994年3月3日本发明涉及识别FK506结合蛋白中的抗原决定簇并且不抑制FK506结合蛋白与FK506之间的结合的单克隆抗体（抗FKBP抗体），用于测定 包括使样品中固定的抗FKBP抗体，酶标记的FK506和FKBP，以及测定酶底物的显色程度和用于测定的试剂盒，使血浆中的FKBP水平升高。 根据本发明，可以更加准确地测定影响FK506的免疫抑制作用的血浆中的FKBP水平，从而能够根据个体患者的情况设定FK506的最佳剂量。

    Method for producing 2-keto-L-gulonic acid
    6.
    发明授权
    Method for producing 2-keto-L-gulonic acid 失效
    2-酮-L-古洛糖酸的制备方法

    公开(公告)号：US5888786A

    公开(公告)日：1999-03-30

    申请号：US943285

    申请日：1997-10-03

    申请人： Mineo Niwa ,  Yoshimasa Saito ,  Yoshinori Ishii ,  Masaru Yoshida ,  Hiromi Hayashi

    发明人： Mineo Niwa ,  Yoshimasa Saito ,  Yoshinori Ishii ,  Masaru Yoshida ,  Hiromi Hayashi

    IPC分类号： C12N9/02 ,  C12N9/04 ,  C12N15/53 ,  C12P7/60 ,  C12N1/00 ,  C12N15/00

    CPC分类号： C12N9/0008 ,  C12N9/0006 ,  C12P7/60 ,  Y10S435/822

    摘要： An expression vector containing both a DNA encoding an L-sorbose dehydrogenase and a DNA encoding an L-sorbosone dehydrogenase; a transformant having an ability to produce 2-keto-L-gulonic acid (hereinafter 2KLGA) at high yields from D-sorbitol, which is prepared by transforming, with said expression vector, a microorganism capable of producing L-sorbose at high yields from D-sorbitol, which has no or low 2KLGA-decomposing activity or a host microorganism having, in addition to the above-mentioned properties, no or low L-idonic acid-producing activity; and a process for producing 2KLGA, which comprises culturing said transformant in a medium containing D-sorbitol. According to the present invention, 2KLGA useful for the production of L-ascorbic acid can be produced with ease and in larger amounts by a single operation of culture.

    摘要翻译： 含有编码L-山梨糖脱氢酶的DNA和编码L-山梨糖酮脱氢酶的DNA的表达载体; 具有从D-山梨醇以高产率生产2-酮-L-古洛糖酸（以下称为2KLGA）的能力的转化体，其通过用所述表达载体转化能以高产率生产L-山梨糖的微生物制备 没有或低的2KLGA分解活性的D-山梨糖醇或除了上述性质之外，没有或低的L-天冬糖酸生产活性的宿主微生物; 以及生产2KLGA的方法，其包括在含有D-山梨糖醇的培养基中培养所述转化体。 根据本发明，用于生产L-抗坏血酸的2KLGA可以通过单次的培养操作容易地和大量地生产。

    L-sorbose dehydrogenase and novel L-sorbosone dehydrogenase obtained from gluconobacter oxydans T-100
    7.
    发明授权
    L-sorbose dehydrogenase and novel L-sorbosone dehydrogenase obtained from gluconobacter oxydans T-100 失效
    L-山梨糖脱氢酶和由氧化葡萄糖葡萄球菌T-100获得的新型L-山梨糖酮脱氢酶

    公开(公告)号：US06197562B1

    公开(公告)日：2001-03-06

    申请号：US09118317

    申请日：1998-07-17

    申请人： Mineo Niwa ,  Yoshimasa Saito ,  Yoshinori Ishii ,  Masaru Yoshida ,  Hiromi Suzuki

    发明人： Mineo Niwa ,  Yoshimasa Saito ,  Yoshinori Ishii ,  Masaru Yoshida ,  Hiromi Suzuki

    IPC分类号： C12N904

    CPC分类号： C12N9/0006 ,  Y10S435/822

    摘要： A novel L-sorbose dehydrogenase (SDH) and a novel L-sorbosone dehydrogenase both derived from Gluconobacter oxydans T-100, a DNA which encodes the SDH and/or SNDH, an expression vector which contains the DNA, a host cell transformed by the expression vector and a process for producing the SDH and/or SNDH, which comprises culturing the host cell in a medium and recovering the SDH and/or SNDH from the resulting culture. The SDH and SNDH of the present invention are useful enzymes having preferable properties for the production of 2-keto-L-gulonic acid, as well as L-ascorbic acid. According to the production method of the present invention, the SDH and SNDH having such preferable properties can be produced in large amounts by genetic engineering.

    摘要翻译： 一种新型的L-山梨糖脱氢酶（SDH）和一种新型的L-山梨糖酮脱氢酶，它们都衍生自氧化葡糖杆菌T-100，一种编码SDH和/或SNDH的DNA，含有该DNA的表达载体，由 表达载体和产生SDH和/或SNDH的方法，其包括在培养基中培养宿主细胞并从所得培养物中回收SDH和/或SNDH。 本发明的SDH和SNDH是生产2-酮-L-古洛糖酸以及L-抗坏血酸的优选性质的有用的酶。 根据本发明的制造方法，可以通过遗传工程大量生产具有优选性能的SDH和SNDH。