公开(公告)号：US20060247862A1

公开(公告)日：2006-11-02

申请号：US10550362

申请日：2003-09-08

申请人： Nicholas Arini ,  Ian Goodyer ,  Alla Zaltsman

发明人： Nicholas Arini ,  Ian Goodyer ,  Alla Zaltsman

IPC分类号： G06F19/00 ,  G06K9/00

CPC分类号： G06K9/00127

摘要： A method of determining cell cycle phase data for cells comprising at least one luminescent reporter capable of emitting radiation, the at least one luminescent reporter comprising a first luminescent reporter which is capable of being indicative of at least one cell cycle phase, said method comprising: storing classification information for classifying individual cells into different cell cycle phases using an automated classification process; receiving image data created by detecting radiation emitted by said at least one luminescent reporter; analyzing said image data to identify object areas in the image data which correspond to individual cells; analyzing said image data, on the basis of said identified object areas, to determine, for a selected cell, one or more measurements including a measurement of a parameter relating to at least a cytoplasmic component of the cell; and applying said classification information to said measurements to classify the selected cell into a selected one of a plurality of sub-populations of cells, each sub-population having cells in a different cell cycle phase.

摘要翻译： 一种确定细胞的细胞周期相位数据的方法，所述细胞包含至少一种能够发射辐射的发光报告基团，所述至少一种发光报告基因包含能够指示至少一个细胞周期相的第一发光报道基因，所述方法包括： 存储用于使用自动分类过程将各个细胞分类成不同的细胞周期阶段的分类信息; 接收通过检测由所述至少一个发光报告器发射的辐射而产生的图像数据; 分析所述图像数据以识别对应于各个单元的图像数据中的对象区域; 基于所述识别的对象区域分析所述图像数据，以针对所选择的小区来确定包括与所述小区的至少细胞质组分相关的参数的测量的一个或多个测量; 以及将所述分类信息应用于所述测量以将所选择的细胞分类为多个细胞亚群中的所选择的一个，每个亚群具有不同细胞周期阶段的细胞。

公开(公告)号：US07369696B2

公开(公告)日：2008-05-06

申请号：US10817213

申请日：2004-04-02

申请人： Nick Arini ,  Alla Zaltsman ,  Ian Goodyer ,  Yuriy Alexandrov ,  Jurek Cybuch ,  Bohdan Soltys ,  Louis Dagenais ,  Liz Roquemore ,  Sam Murphy

发明人： Nick Arini ,  Alla Zaltsman ,  Ian Goodyer ,  Yuriy Alexandrov ,  Jurek Cybuch ,  Bohdan Soltys ,  Louis Dagenais ,  Liz Roquemore ,  Sam Murphy

IPC分类号： G06F19/00 ,  G06F7/08 ,  G06K9/00

CPC分类号： G06K9/00127 ,  G01N15/1475 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/6458 ,  G01N2015/1477 ,  G01N2015/1493

摘要： A method of classifying cells into subpopulations using cell classifying data is described. The method comprises receiving and analyzing image data to identify object areas in the image data to determine, for at least one selected first cell, one or more measurements. A first parameter set is derived from the measurements for the first cell, the first parameter set comprising at least one of said one or more measurements. The first set of cells are classified into subpopulations, and identified to produce first identifying data. Cell classifying data for use in classifying a second set of cells into subpopulations is derived from the first parameter set and the first identifying data. A second set of cells is classified into subpopulations on the basis of one or more measurements taken for cells in the second set of cells, by use of the cell classifying data. The parameter sets of cells may be represented as vectors in an n-dimensional space.

摘要翻译： 描述了使用细胞分类数据将细胞分类为亚群体的方法。 该方法包括接收和分析图像数据以识别图像数据中的对象区域，以便对于至少一个所选择的第一小区来确定一个或多个测量。 从第一单元的测量得到第一参数集，第一参数集包括所述一个或多个测量中的至少一个。 第一组细胞被分类为亚群，并被鉴定以产生第一识别数据。 从第一参数集和第一识别数据导出用于将第二组细胞分类为子群体的细胞分类数据。 基于对第二组细胞中的细胞进行的一次或多次测量，通过使用细胞分类数据将第二组细胞分类为亚群体。 单元的参数集可以表示为n维空间中的向量。

公开(公告)号：US20050207633A1

公开(公告)日：2005-09-22

申请号：US10817213

申请日：2004-04-02

申请人： Nick Arini ,  Alla Zaltsman ,  Ian Goodyer ,  Yuriy Alexandrov ,  Jurek Cybuch ,  Bohdan Soltys ,  Louis Dagenais ,  Liz Roquemore ,  Sam Murphy

发明人： Nick Arini ,  Alla Zaltsman ,  Ian Goodyer ,  Yuriy Alexandrov ,  Jurek Cybuch ,  Bohdan Soltys ,  Louis Dagenais ,  Liz Roquemore ,  Sam Murphy

IPC分类号： G01N15/14 ,  G01N21/64 ,  G06K9/00 ,  G06K9/62

CPC分类号： G06K9/00127 ,  G01N15/1475 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/6458 ,  G01N2015/1477 ,  G01N2015/1493

摘要： A method of classifying cells into subpopulations using cell classifying data is described. The method comprises receiving and analyzing image data to identify object areas in the image data to determine, for at least one selected first cell, one or more measurements. A first parameter set is derived from the measurements for the first cell, the first parameter set comprising at least one of said one or more measurements. The first set of cells are classified into subpopulations, and identified to produce first identifying data. Cell classifying data for use in classifying a second set of cells into subpopulations is derived from the first parameter set and the first identifying data. A second set of cells is classified into subpopulations on the basis of one or more measurements taken for cells in the second set of cells, by use of the cell classifying data. The parameter sets of cells may be represented as vectors in an n-dimensional space.

摘要翻译： 描述了使用细胞分类数据将细胞分类为亚群体的方法。 该方法包括接收和分析图像数据以识别图像数据中的对象区域，以便对于至少一个所选择的第一小区来确定一个或多个测量。 从第一单元的测量得到第一参数集，第一参数集包括所述一个或多个测量中的至少一个。 第一组细胞被分类为亚群，并被鉴定以产生第一识别数据。 从第一参数集和第一识别数据导出用于将第二组细胞分类为子群体的细胞分类数据。 基于对第二组细胞中的细胞进行的一次或多次测量，通过使用细胞分类数据将第二组细胞分类为亚群体。 单元的参数集可以表示为n维空间中的向量。

公开(公告)号：US20070160270A1

公开(公告)日：2007-07-12

申请号：US10561574

申请日：2004-07-28

申请人： Nicholas Arini ,  Ian Goodyer ,  Dietrich Ruehlmann ,  Nicholas Thomas

发明人： Nicholas Arini ,  Ian Goodyer ,  Dietrich Ruehlmann ,  Nicholas Thomas

IPC分类号： G06K9/00

CPC分类号： G01N1/30 ,  G01N2021/6439

摘要： A method of analysing a plurality of biological entities using an imaging apparatus. The method comprises: providing a marker for said plurality of biological entities, said marker being capable of identifying objects within said plurality of biological entities when detected using the imaging apparatus, the method of provision being arranged such that said marker is capable of identifying said objects during a first time period, and said marker is less capable of identifying said objects during a second time period; during the first time period, recording a marked-up image in which spatial definitions of said objects are identifiable from said marker; during the second time period, recording a first image of said plurality of biological entities; and generating a spatial definition for an object in said first image using data derived from said marked-up image.

摘要翻译： 使用成像装置分析多个生物实体的方法。 该方法包括：提供用于所述多个生物实体的标记，当使用成像装置检测时，所述标记能够识别所述多​​个生物实体内的物体，所述提供方法被布置为使得所述标记能够识别所述物体 并且所述标记在第二时间期间不太能够识别所述对象; 在第一时段期间，记录标记图像，其中所述对象的空间定义可从所述标记识别; 在所述第二时间段期间，记录所述多个生物实体的第一图像; 以及使用从所述标记图像导出的数据来生成所述第一图像中的对象的空间定义。

公开(公告)号：US20090213371A1

公开(公告)日：2009-08-27

申请号：US12096908

申请日：2006-12-13

申请人： Ian Goodyer ,  Tracy Ann Nutall ,  Rudi Labarbe ,  Nicholas Salvatore Arini ,  Andrew Seddon ,  Nick Stock ,  Gary Ross ,  Ralf Patz

发明人： Ian Goodyer ,  Tracy Ann Nutall ,  Rudi Labarbe ,  Nicholas Salvatore Arini ,  Andrew Seddon ,  Nick Stock ,  Gary Ross ,  Ralf Patz

IPC分类号： G01J3/28

CPC分类号： G01J3/02 ,  G01J3/0202 ,  G01J3/0218 ,  G01J3/0237 ,  G01J3/024 ,  G01J3/0262 ,  G01J3/0264 ,  G01J3/027 ,  G01J3/0272 ,  G01J3/0286 ,  G01J3/0291 ,  G01J3/06 ,  G01J3/10 ,  G01J3/28 ,  G01N21/274 ,  G01N21/3563 ,  G01N21/359 ,  G01N2201/12707 ,  G01N2201/128 ,  G01N2201/129

摘要： A spectrophotometer has a first photodetector (24) and a second photodetector (25) which is displaced spatially from the first photodetector in the direction of increasing wavelength in the spectrum. At any given time the second photodetector receives light at a wavelength which is substantially greater than that being received simultaneously by the first photodetector at that time. The first photodetector has first range of wavelengths over which it is operable and a first upper operating limit, and the second photodetector has a second range of wavelengths over which it is operable and a second upper operating limit, the second range overlapping the first range and the second upper operating limit being greater than the first upper operating limit. Thus the range of operation is extended, and data in two different ranges is processed simultaneously. The spectrophotometer comprises a housing (1) containing a light source (11), a monochromator (15, 16, 18) and the photodetectors, there being a fibre optic connected to a probe (2) for transmitting light from the light source to a sample to be analysed and receiving light from the sample. Optical components are mounted to a chassis (26) of the housing rigidly, the chassis being connected to the housing by shock absorbing mounts (28, 29). The light source is mounted to the housing by means of an adjuster (24) providing for adjustment laterally with respect to the optical axis of the light source.

摘要翻译： 分光光度计具有第一光电检测器（24）和第二光电检测器（25），该第二光电检测器（25）在光谱中沿着波长增加的方向从第一光电检测器空间位移。 在任何给定时间，第二光电探测器接收的光的波长远远大于在此时由第一光电检测器同时接收的波长。 第一光电探测器具有可操作的第一波长范围和第一上限工作极限，并且第二光电探测器具有可操作的第二波长范围和第二上限工作极限，第二范围与第一范围重叠， 第二上限运行极限大于第一上限运行极限。 因此，扩展了操作范围，同时处理两个不同范围的数据。 分光光度计包括容纳光源（11）的壳体（1），单色仪（15,16,18）和光电检测器，光纤连接到探头（2），用于将光从光源传输到 待分析样品并从样品中接收光。 光学元件刚性地安装到壳体的底盘（26）上，底盘通过减震座（28,29）连接到壳体。 光源通过调节器（24）安装到壳体，调节器（24）相对于光源的光轴横向调整。

公开(公告)号：US20060275847A1

公开(公告)日：2006-12-07

申请号：US10514138

申请日：2003-04-28

申请人： Ian Goodyer ,  Rudi Labarbe ,  Dietrich Ruehlmann ,  Simon Stubbs

发明人： Ian Goodyer ,  Rudi Labarbe ,  Dietrich Ruehlmann ,  Simon Stubbs

IPC分类号： G01N33/554 ,  C12Q1/34 ,  C12Q1/42 ,  C12Q1/26 ,  C12Q1/04

CPC分类号： G01N21/6452 ,  G01N21/6428 ,  G01N21/6458 ,  G01N2021/6419 ,  G01N2021/6421 ,  G02B21/0076 ,  G02B21/008 ,  G02B21/245

摘要： An automated method for measuring the development of a biofilm, containing one or more fluorescent moieties, on a plurality of surfaces using a confocal imaging system including: a) a radiation source system for forming a beam of electromagnetic radiation including one or more wavelengths; b) an optical system for directing and focusing said beam onto one or more planes of the object; c) a detection system for detecting electromagnetic radiation emitted from the object and producing image data; and d) a scanning system for scanning the object in a plurality of planes with the electromagnetic radiation, the method comprising the steps of: i) growing said biofilm on said plurality of surfaces; ii) detecting the presence of said one or more fluorescent moieties within the biofilm by scanning the biofilm with electromagnetic radiation in a plurality of planes and collecting fluorescent emissions to produce a plurality of images; and iii) analysing said images by means of a data processing system under the control of computer software to determine the structure of the biofilm.

摘要翻译： 一种用于使用共焦成像系统在多个表面上测量含有一个或多个荧光部分的生物膜的开发的自动化方法，所述共聚焦成像系统包括：a）用于形成包括一个或多个波长的电磁辐射束的辐射源系统; b）用于将所述光束引导和聚焦到所述物体的一个或多个平面上的光学系统; c）用于检测从物体发射的电磁辐射并产生图像数据的检测系统; 以及d）用于利用所述电磁辐射在多个平面中扫描所述物体的扫描系统，所述方法包括以下步骤：i）在所述多个表面上生长所述生物膜; ii）通过在多个平面中用电磁辐射扫描生物膜并且收集荧光发射以产生多个图像来检测生物膜内所述一个或多个荧光部分的存在; 以及iii）在计算机软件的控制下通过数据处理系统分析所述图像，以确定生物膜的结构。