-
公开(公告)号：US08383039B2
公开(公告)日：2013-02-26
申请号：US13033165
申请日：2011-02-23
申请人： Peng Zhou , Lincoln C. Young , Benjamin Thomas , Zongyuan Chen , Greg Mouchka , Todd Roswech , Gwendolyn Spizz , Rubina Yasmin
发明人： Peng Zhou , Lincoln C. Young , Benjamin Thomas , Zongyuan Chen , Greg Mouchka , Todd Roswech , Gwendolyn Spizz , Rubina Yasmin
CPC分类号： C12Q1/6806 , B01L3/022 , B01L3/5027 , B01L3/502753 , B01L7/52 , B01L2200/0647 , B01L2200/0689 , B01L2200/141 , B01L2200/142 , B01L2300/044 , B01L2300/0681 , B01L2300/0829 , B01L2300/0838 , B01L2300/0864 , B01L2300/0867 , B01L2300/0874 , B01L2300/088 , B01L2300/0887 , B01L2400/043 , B01L2400/0481 , B01L2400/0666 , C12Q1/6846 , C12Q1/686 , C12Q1/6883 , C12Q1/708 , G01N35/1002 , C12Q2565/629
摘要： A self-contained, fully automated, biological assay-performing apparatus includes a housing; a dispensing platform including a controllably-movable reagent dispensing system, disposed in the housing; a reagent supply component disposed in the housing; a pneumatic manifold removably disposed in the housing in a space shared by the dispensing platform, removably coupled to a fluidic transport layer and a plurality of reservoirs, wherein the fluidic transport layer, the reservoirs, and a test sample to be introduced therein are disposed in the housing in the space separate from the dispensing platform; a pneumatic supply system removably coupled to the pneumatic manifold in the housing in a space separate from the dispensing platform; and a control system coupled to at least one of the dispensing platform and the pneumatic supply system, disposed in the housing.
-
公开(公告)号：US20110275058A1
公开(公告)日：2011-11-10
申请号：US13033165
申请日：2011-02-23
申请人： Peng Zhou , Lincoln C. Young , Benjamin Thomas , Zongyuan Chen , Greg Mouchka , Todd Roswech , Gwendolyn Spizz , Rubina Yasmin
发明人： Peng Zhou , Lincoln C. Young , Benjamin Thomas , Zongyuan Chen , Greg Mouchka , Todd Roswech , Gwendolyn Spizz , Rubina Yasmin
CPC分类号： C12Q1/6806 , B01L3/022 , B01L3/5027 , B01L3/502753 , B01L7/52 , B01L2200/0647 , B01L2200/0689 , B01L2200/141 , B01L2200/142 , B01L2300/044 , B01L2300/0681 , B01L2300/0829 , B01L2300/0838 , B01L2300/0864 , B01L2300/0867 , B01L2300/0874 , B01L2300/088 , B01L2300/0887 , B01L2400/043 , B01L2400/0481 , B01L2400/0666 , C12Q1/6846 , C12Q1/686 , C12Q1/6883 , C12Q1/708 , G01N35/1002 , C12Q2565/629
摘要： A self-contained, fully automated, biological assay-performing apparatus includes a housing; a dispensing platform including a controllably-movable reagent dispensing system, disposed in the housing; a reagent supply component disposed in the housing; a pneumatic manifold removably disposed in the housing in a space shared by the dispensing platform, removably coupled to a fluidic transport layer and a plurality of reservoirs, wherein the fluidic transport layer, the reservoirs, and a test sample to be introduced therein are disposed in the housing in the space separate from the dispensing platform; a pneumatic supply system removably coupled to the pneumatic manifold in the housing in a space separate from the dispensing platform; and a control system coupled to at least one of the dispensing platform and the pneumatic supply system, disposed in the housing.
摘要翻译： 独立的，完全自动化的生物测定执行装置包括壳体; 包括设置在所述壳体中的可控制的试剂分配系统的分配平台; 设置在所述壳体中的试剂供应部件; 气动歧管，其可移除地设置在所述壳体中的由所述分配平台共享的空间中，可拆卸地联接到流体输送层和多个储存器，其中所述流体输送层，所述储存器以及待引入其中的待测样品设置在 空间中的壳体与分配平台分离; 气动供给系统，其可拆卸地联接到与分配平台分离的空间中的壳体中的气动歧管; 以及耦合到所述分配平台和所述气动供应系统中的至少一个的控制系统，所述控制系统设置在所述壳体中。
-
公开(公告)号：US09132398B2
公开(公告)日：2015-09-15
申请号：US12249872
申请日：2008-10-10
申请人： Peng Zhou , Lincoln C. Young , Todd Roswech , Gwendolyn Spizz , Zongyuan Chen , Benjamin W. Thomas , Travis Lee
发明人： Peng Zhou , Lincoln C. Young , Todd Roswech , Gwendolyn Spizz , Zongyuan Chen , Benjamin W. Thomas , Travis Lee
CPC分类号： B01F13/0059 , B01F5/0683 , B01F5/0688 , B01F11/0074 , B01L3/50273 , B01L7/52 , B01L2200/027 , B01L2200/0621 , B01L2200/10 , B01L2300/0816 , B01L2300/1816 , B01L2300/1822 , B01L2300/1827 , B01L2300/1844 , B01L2300/185 , B01L2400/0487 , B01L2400/0638 , B01L2400/084
摘要： A microfluidic device for analyzing a sample of interest is provided. The microfluidic device can comprise a microfluidic device body, wherein the microfluidic device body comprises a sample preparation area, a nucleic acid amplification area, a nucleic acid analysis area, and a network of fluid channels. Each of the sample preparation area, the nucleic acid amplification area and the nucleic acid analysis area are fluidly interconnected to at least one of the other two areas by at least one of the fluid channels. Using the microfluidic device, sample preparation can be combined with amplification of a biologically active molecule, and a suitable biological sample can be provided for analysis and/or detection of a molecule of interest. The small-scale apparatus and methods provided are easier, faster, less expensive, and equally efficacious compared to larger scale equipment for the preparation and analysis of a biological sample.
摘要翻译： 提供了用于分析感兴趣样品的微流体装置。 微流体装置可以包括微流体装置主体，其中微流体装置主体包括样品制备区域，核酸扩增区域，核酸分析区域和流体通道网络。 样品制备区域，核酸扩增区域和核酸分析区域中的每一个通过至少一个流体通道流体地互连到另外两个区域中的至少一个区域。 使用微流体装置，样品制备可以与生物活性分子的扩增相结合，并且可以提供合适的生物样品用于分析和/或检测目的分子。 与用于制备和分析生物样品的较大规模设备相比，提供的小型装置和方法更容易，更快，更便宜，并且同样有效。
-
公开(公告)号：US20120115738A1
公开(公告)日：2012-05-10
申请号：US12249872
申请日：2008-10-10
申请人： Peng Zhou , Lincoln C. Young , Todd Roswech , Gwendolyn Spizz , Zongyuan Chen , Benjamin W. Thomas , Travis Lee
发明人： Peng Zhou , Lincoln C. Young , Todd Roswech , Gwendolyn Spizz , Zongyuan Chen , Benjamin W. Thomas , Travis Lee
CPC分类号： B01F13/0059 , B01F5/0683 , B01F5/0688 , B01F11/0074 , B01L3/50273 , B01L7/52 , B01L2200/027 , B01L2200/0621 , B01L2200/10 , B01L2300/0816 , B01L2300/1816 , B01L2300/1822 , B01L2300/1827 , B01L2300/1844 , B01L2300/185 , B01L2400/0487 , B01L2400/0638 , B01L2400/084
摘要： A microfluidic device for analyzing a sample of interest is provided. The microfluidic device can comprise a microfluidic device body, wherein the microfluidic device body comprises a sample preparation area, a nucleic acid amplification area, a nucleic acid analysis area, and a network of fluid channels. Each of the sample preparation area, the nucleic acid amplification area and the nucleic acid analysis area are fluidly interconnected to at least one of the other two areas by at least one of the fluid channels. Using the microfluidic device, sample preparation can be combined with amplification of a biologically active molecule, and a suitable biological sample can be provided for analysis and/or detection of a molecule of interest. The small-scale apparatus and methods provided are easier, faster, less expensive, and equally efficacious compared to larger scale equipment for the preparation and analysis of a biological sample.
摘要翻译： 提供了用于分析感兴趣样品的微流体装置。 微流体装置可以包括微流体装置主体，其中微流体装置主体包括样品制备区域，核酸扩增区域，核酸分析区域和流体通道网络。 样品制备区域，核酸扩增区域和核酸分析区域中的每一个通过至少一个流体通道流体地互连到另外两个区域中的至少一个区域。 使用微流体装置，样品制备可以与生物活性分子的扩增相结合，并且可以提供合适的生物样品用于分析和/或检测目的分子。 与用于制备和分析生物样品的较大规模设备相比，提供的小型装置和方法更容易，更快，更便宜，并且同样有效。
-
公开(公告)号：US08722329B2
公开(公告)日：2014-05-13
申请号：US13400857
申请日：2012-02-21
申请人： Lincoln C. Young , Peng Zhou , Gwendolyn Spizz , Rubina Yasmin
发明人： Lincoln C. Young , Peng Zhou , Gwendolyn Spizz , Rubina Yasmin
CPC分类号： C12N15/1013 , C12Q1/6806
摘要： A method is provided for purifying nucleic acid from a sample in a microfluidic device. The method can be used to purify nucleic acids from any source known in the art that comprises nucleic acids, such as prokaryotic or eukaryotic organisms, viruses, cell, tissues, organs, etc. In a specific example, the tissue is whole blood. The method for purifying nucleic acid may run fully automated in the microfluidic device.
摘要翻译： 提供了一种用于从微流体装置中的样品中纯化核酸的方法。 该方法可用于从本领域已知的任何来源纯化核酸，其包含核酸，例如原核或真核生物，病毒，细胞，组织，器官等。在具体实例中，组织是全血。 纯化核酸的方法可以在微流体装置中完全自动化。
-
公开(公告)号：US20120214168A1
公开(公告)日：2012-08-23
申请号：US13400857
申请日：2012-02-21
申请人： Lincoln C. Young , Peng Zhou , Gwendolyn Spizz , Rubina Yasmin
发明人： Lincoln C. Young , Peng Zhou , Gwendolyn Spizz , Rubina Yasmin
CPC分类号： C12N15/1013 , C12Q1/6806
摘要： A method is provided for purifying nucleic acid from a sample in a microfluidic device. The method can be used to purify nucleic acids from any source known in the art that comprises nucleic acids, such as prokaryotic or eukaryotic organisms, viruses, cell, tissues, organs, etc. In a specific example, the tissue is whole blood. The method for purifying nucleic acid may run fully automated in the microfluidic device.
摘要翻译： 提供了一种用于从微流体装置中的样品中纯化核酸的方法。 该方法可用于从本领域已知的任何来源纯化核酸，其包含核酸，例如原核或真核生物，病毒，细胞，组织，器官等。在具体实例中，组织是全血。 纯化核酸的方法可以在微流体装置中完全自动化。
-
公开(公告)号：US09284613B2
公开(公告)日：2016-03-15
申请号：US13273522
申请日：2011-10-14
申请人： Gwendolyn Spizz , Peng Zhou
发明人： Gwendolyn Spizz , Peng Zhou
CPC分类号： C12Q1/6851 , C12Q1/6806 , C12Q1/689 , C12Q1/707 , C12Q1/708 , C12Q2537/143 , C12Q2561/113 , C12Q2565/501
摘要： Methods and systems for detecting a target nucleic acid using the quantitative capabilities of real-time nucleic acid amplification systems and the multiplexing capabilities of hybridization systems, comprising: identifying a conservative sequence and a distinctive sequence within each target nucleic acid sequence; simultaneously amplifying the conservative region and the distinctive region; monitoring the amplification of the conservative region in real-time; identifying the distinctive region amplicon via multiplexed identification; and performing quantitative multiplexing analysis of the target by combining the real-time monitoring information with the multiplexed identification of the target nucleic acid.
摘要翻译： 使用实时核酸扩增系统的定量能力和杂交系统的复用能力来检测靶核酸的方法和系统，包括：鉴定每个靶核酸序列内的保守序列和独特序列; 同时扩增保守区域和特征区域; 实时监控保守区域的放大; 通过复用识别识别特征区扩增子; 以及通过组合实时监测信息与目标核酸的复用鉴定来进行目标的定量多路复用分析。
-
公开(公告)号：US20120129714A1
公开(公告)日：2012-05-24
申请号：US13273522
申请日：2011-10-14
申请人： Gwendolyn Spizz , Peng Zhou
发明人： Gwendolyn Spizz , Peng Zhou
CPC分类号： C12Q1/6851 , C12Q1/6806 , C12Q1/689 , C12Q1/707 , C12Q1/708 , C12Q2537/143 , C12Q2561/113 , C12Q2565/501
摘要： Methods and systems for detecting a target nucleic acid using the quantitative capabilities of real-time nucleic acid amplification systems and the multiplexing capabilities of hybridization systems, comprising: identifying a conservative sequence and a distinctive sequence within each target nucleic acid sequence; simultaneously amplifying the conservative region and the distinctive region; monitoring the amplification of the conservative region in real-time; identifying the distinctive region amplicon via multiplexed identification; and performing quantitative multiplexing analysis of the target by combining the real-time monitoring information with the multiplexed identification of the target nucleic acid.
摘要翻译： 使用实时核酸扩增系统的定量能力和杂交系统的复用能力来检测靶核酸的方法和系统，包括：鉴定每个靶核酸序列内的保守序列和独特序列; 同时扩增保守区域和特征区域; 实时监控保守区域的放大; 通过复用识别识别特征区扩增子; 以及通过组合实时监测信息与目标核酸的复用鉴定来进行目标的定量复用分析。
-
-
-
-
-
-
-
搜索结果
8 条记录 (耗时 0.037 秒)
