利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

56 条记录 (耗时 0.03 秒)

    Methods for detecting amphiphilic antigens
    7.
    发明授权
    Methods for detecting amphiphilic antigens 失效
    检测两亲性抗原的方法

    公开(公告)号：US5187066A

    公开(公告)日：1993-02-16

    申请号：US479930

    申请日：1990-02-14

    申请人： Martin Becker ,  Nurith Kurn ,  Yen P. Liu ,  Rajesh D. Patel ,  Thomas M. Houts ,  John D. Olson

    发明人： Martin Becker ,  Nurith Kurn ,  Yen P. Liu ,  Rajesh D. Patel ,  Thomas M. Houts ,  John D. Olson

    IPC分类号： G01N33/543 ,  G01N33/569 ,  G01N33/571

    CPC分类号： G01N33/543 ,  G01N33/56927 ,  G01N33/571

    摘要： This invention is to a method for detecting an amphiphilic antigen in a biological sample suspected of containing the amphiphilic antigen, which method comprises providing in combination a hydrophilic solid support modified to have a hydrophobic surface and an assay medium suspected of containing an amphiphilic antigen, incubating the combination under conditions sufficient for the amphiphilic antigen to bind to the hydrophobic surface, and determining the presence or amount of the amphiphilic antigen bound to the hydrophobic surface.

    摘要翻译： 本发明涉及一种用于检测怀疑含有两亲性抗原的生物样品中的两亲性抗原的方法，该方法包括组合改性以具有疏水表面的亲水性固体支持物和怀疑含有两亲性抗原的测定培养基 所述组合在足以使两亲性抗原结合到疏水性表面的条件下，以及确定与疏水性表面结合的两亲性抗原的存在或量。

    Amplification method for polynucleotide assays
    9.
    发明授权
    Amplification method for polynucleotide assays 失效
    多核苷酸测定的扩增方法

    公开(公告)号：US5273879A

    公开(公告)日：1993-12-28

    申请号：US614180

    申请日：1990-11-13

    申请人： Thomas C. Goodman ,  Martin Becker ,  Edwin F. Ullman ,  Samuel Rose

    发明人： Thomas C. Goodman ,  Martin Becker ,  Edwin F. Ullman ,  Samuel Rose

    IPC分类号： C12Q1/68 ,  C12Q1/70 ,  C07H21/02 ,  C07H21/04 ,  C12P19/34

    CPC分类号： C12Q1/701 ,  C12Q1/6813 ,  C12Q1/683 ,  C12Q1/6844 ,  C12Q1/6853 ,  C12Q1/6858

    摘要： A Kit is disclosed for a method for producing multiple copies of a primary polynucleotide sequence located at the 3' terminus of a polynucleotide. The method comprises (a) forming in the presence of nucleoside triphosphates and template-dependent polynucleotide polymerase an extension of a primary polynucleotide sequence hybridized with a template sequence of a single stranded pattern polynucleotide comprising two or more template sequences each containing one or more site specific cleavage sequences, (b) cleaving into fragments said extension at cleavable polynucleotide sequences in the presence of means for specifically cleaving said cleavable polynucleotide sequences when said extension is hybridized with said site specific cleavage sequences, (c) dissociating said fragments, (d) hybridizing said fragments with single stranded pattern polynucleotide, and repeating steps (a)-(d). Steps (a)-(d) may be conducted simultaneously or wholly or partially sequentially. The method may be applied in the detection of a polynucleotide analyte in a sample suspected of containing such analyte to facilitate such detection. Also disclosed are compositions for conducting the method of the invention.

    摘要翻译： 公开了用于产生位于多核苷酸的3'末端的多核苷酸序列的多个拷贝的方法的试剂盒。 该方法包括（a）在核苷三磷酸和模板依赖性多聚核苷酸聚合酶的存在下形成与单链图案多核苷酸的模板序列杂交的主要多核苷酸序列的延伸，所述单链图案多核苷酸包含两个或更多个模板序列，每个模板序列含有一个或多个位点特异性 裂解序列，（b）当所述延伸与所述位点特异性切割序列杂交时，在存在用于特异性切割所述可切割多核苷酸序列的手段的存在下，在可切割多核苷酸序列处切割成片段，（c）解离所述片段，（d）杂交 所述具有单链图案多核苷酸的片段，以及重复步骤（a） - （d）。 步骤（a） - （d）可以同时或全部或部分顺序进行。 该方法可以应用于怀疑含有此类分析物的样品中的多核苷酸分析物的检测以促进这种检测。 还公开了用于进行本发明方法的组合物。

    Homogeneous immunoassays using mutant glucose-6-phosphate dehydrogenases
    10.
    发明授权
    Homogeneous immunoassays using mutant glucose-6-phosphate dehydrogenases 失效
    使用突变型葡萄糖-6-磷酸脱氢酶的均相免疫测定

    公开(公告)号：US06455288B1

    公开(公告)日：2002-09-24

    申请号：US08044857

    申请日：1993-04-08

    申请人： Edward Benjamin Jakobovits ,  Joy L. Silen ,  Mark J. Levy ,  Thomas C. Goodman ,  Martin Becker ,  Edwin F. Ullman ,  Robert M. Caldwell ,  Richard R. Bott ,  Christopher Charles Barnett

    发明人： Edward Benjamin Jakobovits ,  Joy L. Silen ,  Mark J. Levy ,  Thomas C. Goodman ,  Martin Becker ,  Edwin F. Ullman ,  Robert M. Caldwell ,  Richard R. Bott ,  Christopher Charles Barnett

    IPC分类号： C12N1552

    CPC分类号： C12N9/0006 ,  C12Q1/28 ,  G01N33/535 ,  G01N33/56911 ,  G01N33/581 ,  G01N33/78 ,  G01N33/94 ,  G01N2333/904

    摘要： The present invention relates to methods for immunoassay of analytes employing mutant glucose-6-phosphate dehydrogenase (G6PDH) enzymes as labels. In particular, the invention relates-to the use of conjugates of an analyte or analyte analog and a mutant NAD+ dependent G6PDH differing from any precursor G6PDH by the deletion, substitution, or insertion, or any combination thereof of at least one amino acid per subunit. The invention also involves the construction of several mutations in precursor glucose-6-phosphate dehydrogenase (G6PDH) enzymes. Typically, the mutations involve deletion or substitution of one or more lysine residues, or introduction of one or more cysteine residues by insertion of cysteine to precursor G6PDH or substitution of precursor G6PDH amino acids residues with cysteine. The present invention also relates to conjugates of the subject enzymes and specific binding pair members, kits useful in performing the methods of the invention, cell lines producing the subject enzymes, DNA sequences encoding the subject enzymes, and vectors containing DNA encoding the subject enzymes and designed to allow a host cell to produce the subject enzymes.

    摘要翻译： 本发明涉及使用突变型葡萄糖-6-磷酸脱氢酶（G6PDH）酶作为标记免疫测定分析物的方法。 特别地，本发明涉及分离物或分析物类似物和与任何前体G6PDH不同的突变体NAD +依赖性G6PDH通过每个亚基的缺失，取代或插入或其任何组合的至少一个氨基酸的缀合物的用途 。 本发明还涉及前体葡萄糖-6-磷酸脱氢酶（G6PDH）酶的几个突变的构建。 通常，突变涉及一个或多个赖氨酸残基的缺失或取代，或通过将半胱氨酸插入前体G6PDH或用半胱氨酸取代前体G6PDH氨基酸残基引入一个或多个半胱氨酸残基。 本发明还涉及本发明的酶和特异性结合对成员的缀合物，可用于实施本发明方法的试剂盒，产生本发明酶的细胞系，编码本发明酶的DNA序列，和含有编码本发明酶的DNA的载体和 旨在使宿主细胞产生目标酶。