-
公开(公告)号：US11089767B2
公开(公告)日：2021-08-17
申请号：US15942185
申请日：2018-03-30
申请人： Randolph V. Lewis , Lijin Xia , Xiaoli Zhang , Justin A. Jones
发明人： Randolph V. Lewis , Lijin Xia , Xiaoli Zhang , Justin A. Jones
IPC分类号： A01K67/033 , A01K67/04 , C12N15/85 , C07K14/435 , C12P21/02 , C12N15/90
摘要： Transgenic silkworms stably expressing synthetic spider silk genes or composite silkworm/spider silk genes are disclosed. The exogenous spider silk genes are stably intergrated into a defined site of the fibroin heavy chain intron or a fibroin light chain intron of silkworms. Synthetic spider silk proteins and composite spider silk-silkworm genes and proteins are provided. The expression of exogenous spider silk genes is driven by the endogenous fibroin heavy chain promoter, improving the genetic stability of transgenic silkworms. The composite silkworm/spider silk fibers exhibit exceptional mechanical performance, compared to normal silkworm silk fibers and other transgenic silkworm fibers.
-
公开(公告)号：US20180288988A1
公开(公告)日：2018-10-11
申请号：US15942185
申请日：2018-03-30
申请人： Randolph V. Lewis , Lijin Xia , Xiaoli Zhang , Justin A. Jones
发明人： Randolph V. Lewis , Lijin Xia , Xiaoli Zhang , Justin A. Jones
IPC分类号： A01K67/033 , A01K67/04 , C12N15/85 , C12P21/02 , C07K14/435
摘要： Transgenic silkworms stably expressing synthetic spider silk genes or composite silkworm/spider silk genes are disclosed. The exogenous spider silk genes are stably intergrated into a defined site of the fibroin heavy chain intron or a fibroin light chain intron of silkworms. Synthetic spider silk proteins and composite spider silk-silkworm genes and proteins are provided. The expression of exogenous spider silk genes is driven by the endogenous fibroin heavy chain promoter, improving the genetic stability of transgenic silkworms. The composite silkworm/spider silk fibers exhibit exceptional mechanical performance, compared to normal silkworm silk fibers and other transgenic silkworm fibers.
-
公开(公告)号：US20150292120A1
公开(公告)日：2015-10-15
申请号：US14681951
申请日：2015-04-08
CPC分类号： B29C48/022 , B29C48/0018 , B29C48/05 , B29C48/919 , B29K2089/00 , B29L2031/731 , D01D5/06 , D01D5/16 , D01F4/02
摘要： Methods and apparatuses for preparing protein fibers (biofilaments) from recombinant biofilament proteins are disclosed. The methods are particularly useful for spinning fibers of spider silk or silkworm silk proteins from recombinant sources and may be used to spin such fibers for use in the manufacture of industrial and commercial products.
摘要翻译： 公开了从重组生物丝蛋白制备蛋白质纤维（生物丝）的方法和装置。 该方法特别适用于从重组来源纺丝蜘蛛丝或蚕丝蛋白的纤维，并可用于纺丝这种纤维用于制造工业和商业产品。
-
公开(公告)号：US20150202651A1
公开(公告)日：2015-07-23
申请号：US14574235
申请日：2014-12-17
IPC分类号： B05D3/06 , A61K8/64 , A61K35/646 , B01D29/00 , A61L27/22 , G02B1/10 , C08J5/18 , B29C35/08 , A61Q19/00
CPC分类号： B05D3/06 , A23V2002/00 , A61K8/0216 , A61K8/64 , A61K35/646 , A61K2800/10 , A61L27/227 , A61Q19/00 , B01D29/0093 , B29C35/08 , B29K2093/00 , B29L2007/00 , C08J5/18 , C08J2389/00 , G02B1/10 , Y10T428/31971 , Y10T442/20
摘要： Methods for forming useful films using recombinant spider silk protein are discussed. In one embodiment, the method involves dissolving silk protein in a sufficient quantity to form a film suspended in a solvent. The solution is then mixed with a compound selected from water, acids, imidazole crosslinking agents, antibiotics, nanoparticles, surfactants and combinations thereof. Solubilisation of the protein in the solution is effected by microwave exposure. Once solubilized the microwave treated solution is poured onto a suitable substrate and dried in order to result in the final film, the protein may be plant, mammal or bacterially derived. The film has widespread use and can be combined with any one of a number of substrates, examples of which include: a cosmetic composition; a pharmaceutical or medical composition, drug delivery system, artificial cell, contact lens coating, sustained-release drug delivery system, artificial skin graft; food composition; automotive part; aeronautic component; computer or data storage device, building material, textile, filter material, membrane material, nanomaterial, electronic component and combinations thereof.
摘要翻译： 讨论了使用重组蜘蛛丝蛋白形成有用膜的方法。 在一个实施方案中，该方法包括将丝蛋白溶解在足够的量中以形成悬浮在溶剂中的膜。 然后将溶液与选自水，酸，咪唑交联剂，抗生素，纳米颗粒，表面活性剂及其组合的化合物混合。 溶液中蛋白质的溶解是通过微波暴露来实现的。 一旦溶解，将微波处理的溶液倒入合适的底物上并干燥以产生最终的膜，蛋白质可以是植物，哺乳动物或细菌来源的。 该膜具有广泛的用途，并且可以与多种基材中的任一种组合，其实例包括：化妆品组合物; 药物或药物组合物，药物递送系统，人造细胞，隐形眼镜涂层，缓释药物递送系统，人造皮肤移植物; 食物成分; 汽车零件; 航空部件; 计算机或数据存储装置，建筑材料，纺织品，过滤材料，膜材料，纳米材料，电子部件及其组合。
-
公开(公告)号：US20220340622A1
公开(公告)日：2022-10-27
申请号：US17651020
申请日：2022-02-14
申请人： Justin A. JONES , Randolph V. Lewis , Dong Chen , Paula F. Oliveira , Thomas Ian Harris , Brianne Eileen Bell
发明人： Justin A. JONES , Randolph V. Lewis , Dong Chen , Paula F. Oliveira , Thomas Ian Harris , Brianne Eileen Bell
摘要： The present application provides fibers made from recombinant hagfish intermediate filament proteins. Methods of expressing and purifying the proteins, and methods of creating the fibers, are likewise disclosed. The method of purifying protein yields larger quantities of hagfish protein than have been previously achieved, and the fibers have the highest mechanical properties yet recorded for recombinant hagfish protein fibers.
-
公开(公告)号：US20190010195A1
公开(公告)日：2019-01-10
申请号：US15417106
申请日：2017-01-26
IPC分类号： C07K14/435 , A61L27/22 , A61L27/54
摘要： Medical devices made with coatings made from recombinant spider silk proteins are disclosed. Methods to make the devices are also disclosed. Methods for adhering objects to one another using recombinant spider silk proteins are also disclosed.
-
公开(公告)号：US09714273B2
公开(公告)日：2017-07-25
申请号：US14879046
申请日：2015-10-08
申请人： Randolph V. Lewis , Dong Chen , Justin A. Jones , Sreevidhya T. Krishnaji , Paula F. Oliveira , Gargi Bhattacharyya
发明人： Randolph V. Lewis , Dong Chen , Justin A. Jones , Sreevidhya T. Krishnaji , Paula F. Oliveira , Gargi Bhattacharyya
IPC分类号： C12P21/02 , C07K14/435 , C12N15/70
CPC分类号： C07K14/43563 , C07K14/43518 , C12N15/70
摘要： A method of producing synthetic spider silk, including: transforming Escherichia coli with an expression vector; fermenting the transformed E. coli in a culture medium; inducing spider silk protein expression in the cultured E. coli; extracting the spider silk; and purifying the spider silk. Related vectors and genetically modified cells are also disclosed.
-
公开(公告)号：US20160298265A1
公开(公告)日：2016-10-13
申请号：US15096084
申请日：2016-04-11
IPC分类号： D01D5/00 , C08L77/02 , C08L77/04 , C08L33/02 , C08L71/02 , D02G1/02 , C08L61/02 , C08L29/04 , C08L33/06 , C08L33/20 , D01D10/02 , C08L75/04 , C08L69/00
CPC分类号： D01D5/0038 , C08L33/06 , C08L33/20 , C08L61/02 , C08L67/00 , C08L69/00 , C08L71/02 , C08L75/04 , C08L77/02 , C08L77/04 , C08L77/06 , C08L79/02 , C08L89/00 , D01D10/02 , D01F6/90 , D01F6/96 , D02G1/0293 , D02G3/02 , D10B2211/04 , D10B2331/02 , D10B2331/10 , D10B2401/061 , D10B2401/063 , C08L29/04 , C08L31/04
摘要： Synthetic fiber blends and methods for preparing such fibers with spider silk proteins and synthetic and polymers are disclosed.
摘要翻译： 公开了合成纤维混合物和用蜘蛛丝蛋白和合成和聚合物制备这种纤维的方法。
-
公开(公告)号：US20160102125A1
公开(公告)日：2016-04-14
申请号：US14879046
申请日：2015-10-08
申请人： Randolph V. Lewis , Dong Chen , Justin A. Jones , Sreevidhya T. Krishnaji , Paula F. Oliveira , Gargi Bhattacharyya
发明人： Randolph V. Lewis , Dong Chen , Justin A. Jones , Sreevidhya T. Krishnaji , Paula F. Oliveira , Gargi Bhattacharyya
IPC分类号： C07K14/435 , C12N15/70
CPC分类号： C07K14/43563 , C07K14/43518 , C12N15/70
摘要： A method of producing synthetic spider silk, including: transforming Escherichia coli with an expression vector; fermenting the transformed E. coli in a culture medium; inducing spider silk protein expression in the cultured E. coli; extracting the spider silk; and purifying the spider silk. Related vectors and genetically modified cells are also disclosed.
摘要翻译： 一种生产合成蜘蛛丝的方法，包括：用表达载体转化大肠杆菌; 在培养基中发酵转化的大肠杆菌; 在培养的大肠杆菌中诱导蜘蛛丝蛋白表达; 提取蜘蛛丝; 并净化蜘蛛丝。 还公开了相关载体和遗传修饰的细胞。
-
公开(公告)号：US07288391B2
公开(公告)日：2007-10-30
申请号：US10479638
申请日：2002-06-06
申请人： Don A. Roth , Randolph V. Lewis
发明人： Don A. Roth , Randolph V. Lewis
CPC分类号： C07K14/43518 , C12N15/8257
摘要： The present invention relates to the fields of molecular biology and plant biology. Specifically, the invention is directed to the methods for expressing spider silk proteins in plants and the synthesis and purification of spider silk proteins therefrom.
摘要翻译： 本发明涉及分子生物学和植物生物学领域。 具体地说，本发明涉及在植物中表达蜘蛛丝蛋白的方法以及从其中合成和提纯蜘蛛丝蛋白。
-
-
-
-
-
-
-
-
-
搜索结果
23 条记录 (耗时 0.038 秒)
