公开(公告)号：US20090156413A1

公开(公告)日：2009-06-18

申请号：US12320961

申请日：2009-02-10

申请人： Renpei Nagashima ,  Takao Isogai ,  Noriaki Hirayama

发明人： Renpei Nagashima ,  Takao Isogai ,  Noriaki Hirayama

IPC分类号： C40B30/00

CPC分类号： C40B30/04 ,  G01N33/6803 ,  G01N33/6845

摘要： Disclosed in this invention are methods, systems, databases, user-interfaces, software, media, and services useful for evaluating interactions between chemical compounds and proteins and for utilizing the information resulting from such evaluation for the purpose of discovering chemical compounds for medical and other fields. An approach termed “reverse proteomics” is disclosed. This invention generates an enormously large pool of new target proteins for drug discovery, novel methods for designing of new drugs, and a previously unthinkable pool of virtually synthesized small molecules for therapeutic uses. This invention is also applicable, for example, to discovery of substitutes for environmentally hazardous chemicals, more effective agrochemicals, and healthier food additives.

摘要翻译： 在本发明中公开的是用于评估化合物和蛋白质之间的相互作用的方法，系统，数据库，用户接口，软件，媒体和服务，以及为了发现用于医学和其它 领域。 公开了一种称为“反向蛋白质组学”的方法。 本发明产生了一大量用于药物发现的新靶蛋白，新的药物设计新方法和用于治疗用途的以前不可思议的几乎合成的小分子池。 本发明也可应用于例如发现环境危害化学品替代物，更有效的农用化学品和更健康的食品添加剂。

公开(公告)号：US08110662B2

公开(公告)日：2012-02-07

申请号：US11869267

申请日：2007-10-09

申请人： Toshio Ota ,  Takao Isogai ,  Tetsuo Nishikawa ,  Koji Hayashi ,  Kaoru Otsuka ,  Jun-Ichi Yamamoto ,  Shizuko Ishii ,  Tomoyasu Sugiyama ,  Ai Wakamatsu ,  Keiichi Nagai ,  Tetsuji Otsuki ,  Shin-Ichi Funahashi ,  Shoji Miyata ,  Kenji Sobue ,  Kenichiro Hayashi

发明人： Toshio Ota ,  Takao Isogai ,  Tetsuo Nishikawa ,  Koji Hayashi ,  Kaoru Otsuka ,  Jun-Ichi Yamamoto ,  Shizuko Ishii ,  Tomoyasu Sugiyama ,  Ai Wakamatsu ,  Keiichi Nagai ,  Tetsuji Otsuki ,  Shin-Ichi Funahashi ,  Shoji Miyata ,  Kenji Sobue ,  Kenichiro Hayashi

IPC分类号： C07K16/00 ,  A61K39/00

CPC分类号： C07K16/18 ,  A61K38/00 ,  A61K2039/505 ,  A61K2039/53 ,  C07K14/47 ,  C07K14/4702 ,  C12N2799/021

摘要： A cDNA fragment participating in the maintenance of smooth muscle differentiation was isolated using a culture system of chicken gizzard smooth muscle cells, the differential display method and the subtracted hybridization method. Using the obtained cDNA sequence as a query, cDNA sequences of Helix Research Institute (Japanese Patent Application No. 2000-118776) were retrieved, and thus, a novel gene “C-NT2RP3001495” was obtained. The protein encoded by this gene has two WW domains that participate in protein interactions in the N-terminal domain. Evidence suggests that this protein binds to other proteins, and thus regulates the intracellular signal transduction, gene expression, and so on, thereby participating in the maintenance of the differentiation of smooth muscle cells. This protein and compounds regulating the expression thereof are markedly useful in developing drugs for various diseases associated with abnormality in the maintenance of smooth muscle cell differentiation.

摘要翻译： 使用鸡ard平滑肌细胞培养系统，差异显示法和减法杂交法分离参与维持平滑肌分化的cDNA片段。 使用获得的cDNA序列作为查询，检索Helix研究所的cDNA序列（日本专利申请2000-118776），从而得到新的基因“C-NT2RP3001495”。 由该基因编码的蛋白质具有参与N末端结构域中的蛋白质相互作用的两个WW结构域。 证据表明，该蛋白质与其他蛋白质结合，从而调节细胞内信号转导，基因表达等，从而参与维持平滑肌细胞的分化。 该蛋白质和调节其表达的化合物在开发用于维持平滑肌细胞分化异常的各种疾病的药物中显着有用。

公开(公告)号：US20100100979A1

公开(公告)日：2010-04-22

申请号：US12531358

申请日：2008-03-13

申请人： Ai Wakamatsu ,  Junichi Yamamoto ,  Takao Isogai

发明人： Ai Wakamatsu ,  Junichi Yamamoto ,  Takao Isogai

IPC分类号： A61K39/395 ,  C07K14/00 ,  C07H21/04 ,  C12N15/63 ,  C12N5/10 ,  C07K16/00 ,  C12N5/12 ,  A01K67/027 ,  A61P35/00 ,  C12Q1/68

CPC分类号： G01N33/574 ,  A01K2227/105 ,  C07K14/4748 ,  C12Q1/6886 ,  C12Q2600/136 ,  C12Q2600/178

摘要： It is an object of the present invention to provide diagnostic reagents, pharmaceuticals and the like for particular diseases, and providing means that are useful in developing such reagents, pharmaceuticals and the like. The present invention provides a novel polypeptide and a specific partial peptide thereof, as well as a novel polynucleotide and a specific partial nucleotide thereof, that can be used as cancer-specific biomarkers; an expression vector for such a polynucleotide and a specific partial peptide thereof; a transformant incorporating such an expression vector; an antisense molecule, RNAi-inducing nucleic acid (e.g., siRNA), aptamer, or antibody for a cancer-specific biomarker, and a composition comprising the same; a mammalian cell or non-human mammal wherein the expression or a function of a cancer-specific biomarker is regulated; a measuring means (e.g., primer set, nucleic acid probe, antibody, aptamer) for a cancer-specific biomarker, and a reagent comprising them and the like.

摘要翻译： 本发明的目的是为特定疾病提供诊断试剂，药物等，并提供可用于开发这些试剂，药物等的方法。 本发明提供了可用作癌症特异性生物标志物的新型多肽及其特异性部分肽，以及其新型多核苷酸及其特定部分核苷酸; 这种多核苷酸及其特定部分肽的表达载体; 包含这种表达载体的转化体; 用于癌特异性生物标志物的反义分子，RNAi诱导核酸（例如siRNA），适体或抗体，以及包含其的组合物; 哺乳动物细胞或非人哺乳动物，其中调节癌特异性生物标志物的表达或功能; 用于癌特异性生物标志物的测量装置（例如，引物组，核酸探针，抗体，适体）和包含它们的试剂等。

公开(公告)号：US07560541B2

公开(公告)日：2009-07-14

申请号：US11767852

申请日：2007-06-25

申请人： Takao Isogai ,  Tomoyasu Sugiyama ,  Tetsuji Otsuki ,  Ai Wakamatsu ,  Hiroyuki Sato ,  Shizuko Ishii ,  Jun-ichi Yamamoto ,  Yuuko Isono ,  Yuri Hio ,  Kaoru Otsuka ,  Keiichi Nagai ,  Ryotaro Irie ,  Ichiro Tamechika ,  Naohiko Seki ,  Tsutomu Yoshikawa ,  Motoyuki Otsuka ,  Kenji Nagahari ,  Yasuhiko Masuho

发明人： Takao Isogai ,  Tomoyasu Sugiyama ,  Tetsuji Otsuki ,  Ai Wakamatsu ,  Hiroyuki Sato ,  Shizuko Ishii ,  Jun-ichi Yamamoto ,  Yuuko Isono ,  Yuri Hio ,  Kaoru Otsuka ,  Keiichi Nagai ,  Ryotaro Irie ,  Ichiro Tamechika ,  Naohiko Seki ,  Tsutomu Yoshikawa ,  Motoyuki Otsuka ,  Kenji Nagahari ,  Yasuhiko Masuho

IPC分类号： C07H21/04 ,  C12N15/00 ,  C12N1/00

CPC分类号： G01N33/566 ,  C07K14/47 ,  G01N2500/00

摘要： Novel full-length cDNAs are provided. cDNA derived from human have been isolated. The full-length nucleotide sequences of the cDNA and amino acid sequences encoded by the nucleotide sequences have been determined. Because the cDNA of the present invention are full-length and contain the translation start site, they provide information useful for analyzing the functions of the polypeptide.

摘要翻译： 提供新的全长cDNA。 已经分离了源自人的cDNA。 已经确定了由核苷酸序列编码的cDNA和氨基酸序列的全长核苷酸序列。 因为本发明的cDNA是全长的并含有翻译起始位点，所以它们提供了可用于分析多肽功能的信息。

公开(公告)号：US20050214813A1

公开(公告)日：2005-09-29

申请号：US11013400

申请日：2004-12-17

申请人： Toshio Ota ,  Takao Isogai ,  Tetsuo Nishikawa ,  Yuri Hio ,  Mayako Yamazaki ,  Susumu Satoh ,  Hiroyuki Arakawa ,  Masahiko Morita

发明人： Toshio Ota ,  Takao Isogai ,  Tetsuo Nishikawa ,  Yuri Hio ,  Mayako Yamazaki ,  Susumu Satoh ,  Hiroyuki Arakawa ,  Masahiko Morita

IPC分类号： A61K38/00 ,  A61P25/00 ,  A61P25/28 ,  C07K14/47 ,  C07K14/505 ,  C07K14/52 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N15/12 ,  C12N15/16 ,  C12P21/08 ,  C12Q1/68 ,  C07H21/04 ,  C12N9/64

CPC分类号： C07K14/4702 ,  A01K2217/05 ,  A61K38/00 ,  C07K14/4711 ,  C07K14/4747 ,  C07K14/505 ,  C07K14/524

摘要： The present invention provides: proteins suppressing or promoting the aggregation or deposition of amyloid-β protein; polynucleotides encoding the proteins; a method for screening a compound suppressing or promoting the aggregation or deposition of amyloid-β protein; and therapeutic agents for treating or preventing Alzheimer's diseases comprising a compound that regulates the activity of a protein suppressing or promoting the aggregation or deposition of amyloid-β protein.

摘要翻译： 本发明提供：抑制或促进淀粉样蛋白-β蛋白的聚集或沉积的蛋白质; 编码蛋白质的多核苷酸; 筛选抑制或促进淀粉样蛋白-β蛋白的聚集或沉积的化合物的方法; 以及用于治疗或预防阿尔茨海默病的治疗剂，其包含调节蛋白质抑制或促进淀粉样蛋白-β蛋白的聚集或沉积的活性的化合物。

公开(公告)号：US5602016A

公开(公告)日：1997-02-11

申请号：US361708

申请日：1994-12-22

申请人： Takao Isogai ,  Hiroki Ono ,  Hitoshi Kojo

发明人： Takao Isogai ,  Hiroki Ono ,  Hitoshi Kojo

IPC分类号： C12N1/21 ,  C12N9/06 ,  C12N15/53 ,  C12P35/00 ,  C12N9/02 ,  C12N15/35 ,  C12N15/70

CPC分类号： C12N9/0024 ,  C12P35/00

摘要： D-amino acid oxidase (DAO) originated from Fusarium solani M-0718, a DNA compound encoding the oxidase, expression vectors containing said DNA compound are provided. Esherichia coli transformants capable of producing recombinant DAO in high level are also provided.

摘要翻译： 提供源自茄病菌M-0718的D-氨基酸氧化酶（DAO），其是编码氧化酶的DNA化合物，含有所述DNA化合物的表达载体。 还提供了能够生产高水平重组DAO的大肠杆菌转化体。

公开(公告)号：US5132218A

公开(公告)日：1992-07-21

申请号：US467506

申请日：1990-01-19

申请人： Takao Isogai ,  Masaru Yoshida

发明人： Takao Isogai ,  Masaru Yoshida

IPC分类号： C12N15/09 ,  C12N1/20 ,  C12N1/21 ,  C12N15/00 ,  C12N15/68 ,  C12N15/80 ,  C12R1/19 ,  C12R1/645 ,  C12R1/865

CPC分类号： C12N15/80 ,  C12N15/68 ,  Y10S435/911 ,  Y10S435/942

摘要： The present invention relates to new hybrid plasmids useful as vectors in recombinant DNA technology, in which Acremonium chrysogenum or Saccharomyces cerevisiae is used as a host, and to microorganisms bearing the hybrid plasmids.partially digesting a chromosomal DNA of Acremonium chrysogenum ATCC 11550 with restriction enzyme Sau 3A, said autonomous replication sequence having a molecular size of about 1.39 Kbp, and having the restriction maps set forth in FIGS. 4-6.

公开(公告)号：US20070020637A1

公开(公告)日：2007-01-25

申请号：US10760320

申请日：2004-01-21

申请人： Takao Isogai ,  Tomoyasu Sugiyama ,  Tetsuji Otsuki ,  Al Wakamatsu ,  Hiroyuki Sato ,  Shizuko Ishii ,  Junichi Yamamoto ,  Yuko Isono ,  Keiichi Nagai ,  Ryotaro Irie

发明人： Takao Isogai ,  Tomoyasu Sugiyama ,  Tetsuji Otsuki ,  Al Wakamatsu ,  Hiroyuki Sato ,  Shizuko Ishii ,  Junichi Yamamoto ,  Yuko Isono ,  Keiichi Nagai ,  Ryotaro Irie

IPC分类号： C12Q1/68 ,  C07H21/04

CPC分类号： C07K14/47

摘要： Novel full-length cDNAs are provided. 2,495 cDNA derived from human have been isolated. The full-length nucleotide sequences of the cDNA and amino acid sequences encoded by the nucleotide sequences have been determined. Because the cDNA of the present invention are full-length and contain the translation start site, they provide information useful for analyzing the functions of the polypeptide.

摘要翻译： 提供新的全长cDNA。 已经分离出来自人的2,495个cDNA。 已经确定了由核苷酸序列编码的cDNA和氨基酸序列的全长核苷酸序列。 因为本发明的cDNA是全长的并含有翻译起始位点，所以它们提供了可用于分析多肽功能的信息。

公开(公告)号：US20060148030A1

公开(公告)日：2006-07-06

申请号：US10507700

申请日：2003-03-25

申请人： Hitoshi Kojo ,  Kaoru Tajima ,  Masao Fukagawa ,  Shintaro Nishimura ,  Takao Isogai

发明人： Hitoshi Kojo ,  Kaoru Tajima ,  Masao Fukagawa ,  Shintaro Nishimura ,  Takao Isogai

IPC分类号： C12P21/06 ,  C07H21/04 ,  C07K14/72

CPC分类号： C07K14/70567 ,  A01K2217/05 ,  A61K38/00 ,  A61K48/00 ,  C12Q1/6897 ,  G01N33/6875 ,  G01N2333/70567 ,  G01N2333/723 ,  G01N2500/00

摘要： The present invention relates to novel nuclear receptor ERRγ3. Although ERRγ3 itself lacks a DNA binding domain, it comprises the function of enhancing the transcriptional activation function of arbitrary nuclear receptors, such as ERR, ER, or TR. Moreover, the present inventors found that, like ERRγ3, the known proteins ERRγ1 and ERRγ2 also comprise the function of enhancing the transcriptional activation function of other nuclear receptors. Thus, the present invention provides methods for evaluating the regulatory function of these ERRγ subtypes in enhancing the transcriptional activation function of other nuclear receptors, and screening methods based on these evaluation methods.

摘要翻译： 本发明涉及新的核受体ERRγAM3。 尽管ERRgamma3本身缺乏DNA结合结构域，但其包含增强任意核受体如ERR，ER或TR的转录激活功能的功能。 此外，本发明人发现，与ERRγAM3一样，已知蛋白质ERRgamma1和ERRgamma2还包含增强其他核受体的转录激活功能的功能。 因此，本发明提供了用于评估这些ERRγ亚型在增强其他核受体的转录激活功能中的调节功能的方法，以及基于这些评价方法的筛选方法。

公开(公告)号：US20050250144A1

公开(公告)日：2005-11-10

申请号：US11109156

申请日：2005-04-19

申请人： Toshio Ota ,  Takao Isogai ,  Tetsuo Nishikawa ,  Koji Hayashi ,  Kaoru Otsuka ,  Jun-Ichi Yamamoto ,  Shizuko Ishii ,  Tomoyasu Sugiyama ,  Ai Wakamatsu ,  Keiichi Nagai ,  Tetsuji Otsuki ,  Shin-Ichi Funahashi ,  Chiaki Senoo ,  Jin-Ichi Nezu

发明人： Toshio Ota ,  Takao Isogai ,  Tetsuo Nishikawa ,  Koji Hayashi ,  Kaoru Otsuka ,  Jun-Ichi Yamamoto ,  Shizuko Ishii ,  Tomoyasu Sugiyama ,  Ai Wakamatsu ,  Keiichi Nagai ,  Tetsuji Otsuki ,  Shin-Ichi Funahashi ,  Chiaki Senoo ,  Jin-Ichi Nezu

IPC分类号： A61K38/00 ,  C07K14/47 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N15/12 ,  C12Q1/68 ,  C07H21/04 ,  C12N9/12 ,  C12N9/16 ,  C12N15/09 ,  C12P21/06

CPC分类号： C07K14/4702 ,  A61K38/00 ,  A61K2039/505 ,  A61K2039/53 ,  C07K14/47 ,  C12N2799/021

摘要： Selection of clones having the kinase and/or phosphatase-like structure from clones which had been isolated and the structures thereof had been determined in the Helix Research Institute (helix clones; Japanese Patent Application No. 2000-183767) was conducted. Two novel genes were provided by carrying out homology search for all the helix clones by using the amino acid sequences of known kinases and phosphatases as queries. The genes are expected to be involved in intracellular signal transduction. The physiological functions of the inventive genes can be tested by using reporter gene assay systems capable of detecting signal transduction. The proteins of the present invention are useful as target molecules in drug discovery and in the development of new pharmaceuticals.

摘要翻译： 已经在螺旋研究所（螺旋克隆，日本专利申请2000-183767）中确定了已经分离的克隆的克隆和/或磷酸酶样结构的克隆及其结构的选择。 通过使用已知的激酶和磷酸酶的氨基酸序列作为查询，通过对所有螺旋克隆进行同源性检索来提供两个新基因。 这些基因预期参与细胞内信号转导。 可以通过使用能够检测信号转导的报告基因测定系统来测试本发明基因的生理功能。 本发明的蛋白质可用作药物发现和开发新药物中的靶分子。