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1.发明授权Specific binding analytical and separation methods using carboxy containing polymers 失效使用含羧基聚合物的特异性结合分析和分离方法
公开(公告)号：US5262297A
公开(公告)日：1993-11-16
申请号：US876672
申请日：1992-04-30
申请人： Richard C. Sutton , Susan J. Danielson , John B. Findlay , Fred. T. Oakes , Marsha D. B. Oenick , Ignazio S. Ponticello , Harold C. Warren, III
发明人： Richard C. Sutton , Susan J. Danielson , John B. Findlay , Fred. T. Oakes , Marsha D. B. Oenick , Ignazio S. Ponticello , Harold C. Warren, III
IPC分类号： C12Q1/68 , C12Q1/70 , G01N33/543 , G01N33/545 , G01N83/545 , G01N33/546 , G01N33/547
CPC分类号： G01N33/54313 , C12Q1/6876 , C12Q1/701 , C12Q1/703 , G01N33/545 , Y10S436/804 , Y10S436/814 , Y10S436/817 , Y10S436/818 , Y10S436/824
摘要： Specific binding methods are used for diagnostic assays and purification separations whereby the specific binding capture reagent is prepared from copolymers having highly reactive carboxy groups. These groups are extended from the polymer surface with a linking group having from 8 to 50 atoms in the chain and two or more alkylene, arylene, alkylenearylene or arylenealkylene groups. To these reactive groups is attached a biologically active substance such as a protein or oligonucleotide which then participates in the diagnostic assays or purification separation methods.
摘要翻译： 特异性结合方法用于诊断测定和纯化分离，由此特异性结合捕获试剂由具有高反应性羧基的共聚物制备。 这些基团从聚合物表面延伸，在链中具有8至50个原子的连接基团和两个或更多个亚烷基，亚芳基，亚烷基亚芳基或亚芳基亚烷基。 将这些反应性基团连接到生物活性物质如蛋白质或寡核苷酸上，然后参与诊断测定或纯化分离方法。
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2.发明授权Biologically active reagents prepared from carboxy-containing polymer, analytical element and methods of use 失效含羧基聚合物制备的生物活性试剂，分析元素和使用方法
公开(公告)号：US5147777A
公开(公告)日：1992-09-15
申请号：US539774
申请日：1990-06-18
申请人： Richard C. Sutton , Susan J. Danielson , John B. Findlay , Fred T. Oakes , Marsha D. B. Oenick , Ignazio S. Ponticello , Harold C. Warren, III
发明人： Richard C. Sutton , Susan J. Danielson , John B. Findlay , Fred T. Oakes , Marsha D. B. Oenick , Ignazio S. Ponticello , Harold C. Warren, III
IPC分类号： C08F8/00 , C08F246/00 , C12Q1/68 , C12Q1/70 , G01N33/53 , G01N33/543 , G01N33/545
CPC分类号： G01N33/54313 , C12Q1/6876 , C12Q1/701 , C12Q1/703 , G01N33/545 , Y10S436/805 , Y10T428/2991 , Y10T428/2998
摘要： Biologically active reagents are prepared from particles of copolymers having highly reactive carboxy or equivalent groups. The reagents are prepared by covalently attaching biologically active substances, for example antibodies, to the particles, directly or indirectly through highly reactive carboxy groups on the particle surface. These reagents are used to advantage in analytical elements, methods for the detection of specific binding ligands (such as immunological species) and immunoassays, and in purification methods as affinity chromatography reagents.
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3.发明授权Method of preparing biologically active reagents from succinimide-containing polymers, analytical element and methods of use 失效从含琥珀酰亚胺的聚合物制备生物活性试剂的方法，分析元件和使用方法
公开(公告)号：US5308749A
公开(公告)日：1994-05-03
申请号：US646303
申请日：1991-01-25
IPC分类号： C12Q1/68 , G01N33/543 , G01N33/545 , G01N33/531 , G01N33/546
CPC分类号： G01N33/54313 , G01N33/54386 , Y10S524/90 , Y10T428/2991 , Y10T428/2998
摘要： Biologically active reagents are prepared from particles of copolymers having highly active succinimid groups. The reagents are prepared by covalently attaching biologically active substances, for example antibodies, to the particles, directly or indirectly through amide groups by displacement of highly active succinimid groups on the particle surface. These reagents are used to advantage in analytical elements, methods for the detection of specific binding ligands (such as immunological species) and immunoassays, and in purification methods such as affinity chromatography.
摘要翻译： 生物活性试剂由具有高活性琥珀酰亚胺基团的共聚物颗粒制备。 试剂通过将颗粒表面上的高活性琥珀酰亚胺基团置换而直接或间接地通过酰胺基团将生物活性物质例如抗体共价连接而制备。 这些试剂用于分析元件，检测特异性结合配体（例如免疫物质）和免疫测定的方法以及纯化方法如亲和层析中。
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公开(公告)号：US5200462A
公开(公告)日：1993-04-06
申请号：US646132
申请日：1991-01-25
IPC分类号： C08F212/06 , C08F20/34 , C08F22/36 , C08F212/00 , C08F212/14 , C08F220/36 , C08F222/40 , C08F246/00 , C08L25/04 , C08L33/04 , C08L33/14 , G01N33/545
CPC分类号： C08F212/14 , C08F246/00 , G01N33/545
摘要： Water-insoluble, noncrosslinking, nonporous copolymers are provided which have recurring units derived from:(a) from 0 to about 99.9 mole percent of one or more ethylenically unsaturated polymerizable oleophillic monomers which provide hydrophobicity to said copolymer, provided that none of said monomers are crosslinking monomers,(b) from about 0.1 to 100 mole percent of one or more ethylenically unsaturated polymerizable monomers having a succinimidoxycarbonyl group, and(c) from 0 to about 10 mole percent of one or more ionic or polar hydrophilic ethylenically unsaturated polymerizable monomers. These copolymers have a variety of uses, including diagnostic assays.
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公开(公告)号：US5298403A
公开(公告)日：1994-03-29
申请号：US851439
申请日：1992-03-16
申请人： Susan J. Danielson , Barbara A. Brummond , Marsha D. B. Oenick , Ignazio S. Ponticello , David A. Hilborn
发明人： Susan J. Danielson , Barbara A. Brummond , Marsha D. B. Oenick , Ignazio S. Ponticello , David A. Hilborn
IPC分类号： G01N33/532 , G01N33/94 , C07D235/30 , C12N9/02 , C12N9/08 , C12Q1/00
CPC分类号： G01N33/948 , G01N33/532 , G01N33/94
摘要： The invention is directed to labeled drug hapten analogues comprising:(A) a label, of the type used in immunoassays, having an amine or sulfhydryl group;(B) a drug hapten nucleus selected from barbiturates or hydantoins and(C) a linking chain linking the 3-position of the drug hapten nucleus to the label through a carbonyl bridge.
摘要翻译： 本发明涉及标记的药物半抗原类似物，包括：（A）在免疫测定中使用的具有胺或巯基的类型的标记; （B）选自巴比妥酸盐或乙内酰脲的药物半抗原核，（C）通过羰基桥连接药物半抗原核的3位与衔接标记的连接链。
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公开(公告)号：US5284948A
公开(公告)日：1994-02-08
申请号：US851435
申请日：1992-03-16
IPC分类号： G01N33/535 , C07D403/12 , G01N33/532 , G01N33/94 , C07D403/00 , C07D233/72 , C07D239/02
CPC分类号： C07D403/12 , G01N33/532 , G01N33/94 , G01N33/948
摘要： The invention is directed to new drug hapten analogues comprising:(A) an active ester group;(B) a drug hapten nucleus selected from a hydantoin nucleus or a barbiturate nucleus and(C) a linking chain linking the 3-position of the drug hapten nucleus to the active ester group.
摘要翻译： 本发明涉及新的药物半抗原类似物，其包括：（A）活性酯基; （B）选自乙内酰脲核或巴比妥酸核的药物半抗原核和（C）连接药物半抗原核的3位与活性酯基的连接链。
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公开(公告)号：US5601994A
公开(公告)日：1997-02-11
申请号：US235114
申请日：1994-04-28
申请人： Marsha D. B. Oenick , Ignazio S. Ponticello , Susan J. Danielson , Barbara A. Brummond , David A. Hilborn
发明人： Marsha D. B. Oenick , Ignazio S. Ponticello , Susan J. Danielson , Barbara A. Brummond , David A. Hilborn
IPC分类号： G01N33/535 , G01N33/532 , G01N33/543 , G01N33/94
CPC分类号： G01N33/532 , G01N33/54386 , G01N33/94 , G01N33/948 , Y10S436/816
摘要： The invention is directed to labeled drug hapten analogues comprising:(A) a label, of the type used in immunoassays, having an amine or sulfhydryl group;(B) a drug hapten nucleus selected from barbiturates or hydantoins and(C) a linking chain linking the 3-position of the drug hapten nucleus to the label through a carbonyl bridge.
摘要翻译： 本发明涉及标记的药物半抗原类似物，包括：（A）在免疫测定中使用的具有胺或巯基的类型的标记; （B）选自巴比妥酸盐或乙内酰脲的药物半抗原核，（C）通过羰基桥连接药物半抗原核的3位与衔接标记的连接链。
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8.发明授权Biologically active reagent prepared from aldehyde-containing polymer, test kit, analytical element and methods of use 失效由含醛聚合物，试剂盒，分析元素和使用方法制备的生物活性试剂
公开(公告)号：US5401633A
公开(公告)日：1995-03-28
申请号：US955167
申请日：1992-10-01
IPC分类号： G01N33/50 , C12Q1/68 , G01N33/543 , G01N33/545 , G01N33/68 , G01N33/546
CPC分类号： C12Q1/6834 , G01N33/54313 , G01N33/54386 , G01N33/545
摘要： A biologically active reagent is prepared by reacting a biologically active substance covalently to a polymeric particle having pendant aldehyde groups. Such groups are provided by a polymerizable monomer represented by the structure: ##STR1## wherein Ar is arylene, R and R.sup.1 are independently hydrogen, halo or lower alkyl, R.sup.2 and R.sup.3 are independently alkylene of 1 to 4 carbon atoms in the chain, R.sup.4 is arylene, m is 0 or 1, and n is an integer of 1 to 4. These reagents can be used in a variety of specific binding and affinity purification methods, and in both solution assays and dry analytical elements.
摘要翻译： 通过将生物活性物质共价反应到具有侧基醛基的聚合物颗粒来制备生物活性试剂。 这样的基团由以下结构表示的可聚合单体提供：其中Ar是亚芳基，R和R 1独立地是氢，卤素或低级烷基，R 2和R 3独立地是链中具有1至4个碳原子的亚烷基，R 4 是亚芳基，m是0或1，n是1至4的整数。这些试剂可以用于各种特异性结合和亲和纯化方法，以及在溶液测定和干燥分析元件中。
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9.发明授权Methods for capture and selective release of nucleic acids using weakly basic polymer and amplification of same 失效使用弱碱性聚合物捕获和选择性释放核酸的方法及其扩增
公开(公告)号：US5582988A
公开(公告)日：1996-12-10
申请号：US306870
申请日：1994-09-15
申请人： John W. Backus , Tobias E. Ekeze , Jerome C. Swartz , Richard C. Sutton , Ignazio S. Ponticello , JoAnne H. Kerschner , John B. Findlay
发明人： John W. Backus , Tobias E. Ekeze , Jerome C. Swartz , Richard C. Sutton , Ignazio S. Ponticello , JoAnne H. Kerschner , John B. Findlay
IPC分类号： C12N15/09 , C07H21/04 , C07K14/045 , C07K14/16 , C07K14/35 , C08F20/34 , C08F20/60 , C12N15/10 , C12P19/34 , C12Q1/68 , C12Q1/70 , G01N33/543 , G01N33/569
CPC分类号： C12N15/1003 , C07K14/005 , C07K14/35 , C08F20/34 , C08F20/60 , C12N15/1006 , C12Q1/6806 , C12N2710/16122 , C12N2740/16022
摘要： Nucleic acids can be made available for amplification or other treatment after lysis by contacting the lysate with specific weakly basic polymers to form a precipitate with the nucleic acids at acidic pH. After removing non-precipitated materials, the pH is then made basic, thereby releasing the nucleic acids from the polymer. This method for preparing specimen samples is simple and quite rapid, and the released nucleic acids can be further treated in hybridization assays or amplification procedures. The weakly basic polymers are water-soluble and cationic at acidic pH, but neutral in charge at basic pH.
摘要翻译： 通过使溶解产物与特定的弱碱性聚合物接触以在酸性pH下与核酸形成沉淀物，使核酸可用于扩增或裂解后的其它处理。 除去非沉淀物后，将pH调至碱性，从而将聚合物中的核酸释放出来。 制备标本样品的这种方法是简单且相当快速的，并且释放的核酸可以在杂交测定或扩增程序中进一步处理。 弱碱性聚合物在酸性pH下为水溶性和阳离子型，而在碱性pH下为负电荷。
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公开(公告)号：US5527709A
公开(公告)日：1996-06-18
申请号：US410548
申请日：1995-03-23
IPC分类号： C12N9/08 , G01N33/78 , G01N33/545
CPC分类号： C12N9/0065 , G01N33/78 , Y10S436/817
摘要： The invention is directed to an immunoassay for a thyronine derivative comprising:A. contacting a liquid sample, containing a thyronine derivative, with a labeled thyronine hapten analogue of the derivative in the presence of antibodies for the thyronine derivative under conditions that promote the formation of antibody-thyronine immunocomplexes; andB. determining the bound or unbound quantity of the thyronine derivative in the liquid; characterized in that the labeled thyronine derivative comprises:(i) a label, of the type used in immunoassays, having an amine or sulfhydyl group;(ii) a thyronine nucleus and(iii) a linking chain linking the label to the thyronine nucleus.
摘要翻译： 本发明涉及一种对于神钢衍生物的免疫测定，其包括：A.在促进形成甲状腺激素的条件下，在含有甲状腺导向抗体的抗体存在下，将含有甲状腺衍生物的液体样品与衍生物的标记的甲状腺恶性半抗原类似物接触 抗体 - 甲状腺免疫复合物; 和B.确定液体中的甲状腺衍生物的结合或未结合量; 其特征在于，所述标记的甲状腺衍生物包含：（i）在免疫测定中使用的具有胺或巯基的类型的标签; （ii）神经细胞核和（iii）连接标签与陀螺质核的连接链。
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