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    Recombinant bacteria and the uses thereof for producing ethanol
    7.
    发明授权
    Recombinant bacteria and the uses thereof for producing ethanol 有权
    重组细菌及其用于生产乙醇的用途

    公开(公告)号：US09034619B2

    公开(公告)日：2015-05-19

    申请号：US13319526

    申请日：2010-05-12

    Applicant: Jacques Biton ,  Esther Gerber

    Inventor： Jacques Biton ,  Esther Gerber

    IPC: C12N9/88 ,  C12N15/74 ,  C12N9/04 ,  C12P7/06

    CPC classification number: C12P7/065 ,  C12N9/0006 ,  C12N9/88 ,  C12N15/74 ,  C12Y101/01001 ,  C12Y401/01001 ,  Y02E50/17

    Abstract: The present invention relates to recombinant bacteria and the uses thereof, particularly for the production of ethanol. The invention also relates to methods for the production of such bacteria, as well as to nucleic acid constructs suitable for such production. The invention specifically relates to bacteria lacking a functional LDH gene and/or containing a recombinant nucleic acid encoding a PDC and ADH. The bacteria of this invention may be produced from any stress-resistant bacteria.

    Abstract translation: 本发明涉及重组细菌及其用途，特别是用于生产乙醇。 本发明还涉及用于生产这种细菌的方法，以及适用于这种生产的核酸构建体。 本发明具体涉及缺乏功能性LDH基因和/或含有编码PDC和ADH的重组核酸的细菌。 本发明的细菌可以由任何抗应激细菌产生。

    Method of identifying glycosyl transferase binding compounds
    8.
    发明申请
    Method of identifying glycosyl transferase binding compounds 审中-公开
    鉴定糖基转移酶结合化合物的方法

    公开(公告)号：US20050026214A1

    公开(公告)日：2005-02-03

    申请号：US10489034

    申请日：2002-09-02

    Applicant: Jacques Biton ,  Francois Michoux ,  Jean-Noel Veltz ,  Jacques Dumas

    Inventor： Jacques Biton ,  Francois Michoux ,  Jean-Noel Veltz ,  Jacques Dumas

    IPC: G01N33/50 ,  A61K45/00 ,  A61P31/04 ,  C12N9/10 ,  C12N15/09 ,  C12Q1/48 ,  G01N33/15 ,  G01N33/573 ,  G01N33/68 ,  G01N33/53

    CPC classification number: G01N33/6845 ,  C12Q1/48 ,  G01N33/68

    Abstract: The present invention relates to a method of identifying, screening or selecting a compound which binds to the transglycosylation site of a recombinant glycosyl transferase, comprising the steps consisting in: a) bringing said compound into contact with said recombinant protein before, after or at the same time as said recombinant protein is brought into contact with a transglycosylation activity inhibitor, said inhibitor being labeled with a label that generates a direct or indirect signal, b) studying said signal linked to said recombinant protein, the binding of said compound to the transglycosylation site being deduced from the difference between the signal obtained in step b) and the signal obtained in the absence of said compound.

    Abstract translation: 本发明涉及鉴定，筛选或选择与重组糖基转移酶的转糖基化位点结合的化合物的方法，其包括以下步骤：a）使所述化合物与所述重组蛋白质在之前或之后接触 与所述重组蛋白质与转糖基化活性抑制剂接触的同时，所述抑制剂用产生直接或间接信号的标记进行标记，b）研究与所述重组蛋白相连的所述信号，所述化合物与转糖基化的结合 从步骤b）中获得的信号与在不存在所述化合物的情况下获得的信号之间的差异推断出位点。

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