公开(公告)号：US20050246782A1

公开(公告)日：2005-11-03

申请号：US10508808

申请日：2003-03-24

申请人： Robert Etches ,  Robert Kay ,  Philip Leighton ,  Lei Zhu

发明人： Robert Etches ,  Robert Kay ,  Philip Leighton ,  Lei Zhu

IPC分类号： A01K67/027 ,  C07K14/705 ,  C07K16/02 ,  C12N5/06 ,  C12N15/85

CPC分类号： C07K16/461 ,  A01K67/0275 ,  A01K67/0278 ,  A01K2207/15 ,  A01K2217/00 ,  A01K2217/075 ,  A01K2227/30 ,  A01K2267/01 ,  C07K14/70503 ,  C07K16/02 ,  C07K2317/21 ,  C12N15/8509 ,  C12N2800/204 ,  C12N2800/30

摘要： Human antibodies are produced in transgenic chickens having functional genetic components of the human immune system stably integrated in the genome. Techniques are described to integrate unrearranged human heavy and light chain immunoglobulin loci into the genome of the transgenic chicken. The functional description of the endogenous immunoglobulin loci yields an animal whose antibody repertoire in response to antigen is fully human. In the preferred embodiment, the human immunoglobulin locus is at least as large as the native human locus and exhibits functional class switching to yield IgG isotype antibodies. In addition to monoclonal antibodies secreted from immortalized B cell populations, polyclonal antibodies may be obtained from eggs laid by the transgenic chickens of the invention.

摘要翻译： 在具有稳定整合在基因组中的人免疫系统的功能性遗传成分的转基因鸡中产生人抗体。 描述了将未经排列的人重链和轻链免疫球蛋白基因座整合到转基因鸡的基因组中的技术。 内源性免疫球蛋白基因座的功能描述产生一种抗体对抗原作出全部反应的动物。 在优选的实施方案中，人免疫球蛋白基因座至少与天然人基因座一样大，并显示功能类转换以产生IgG同种型抗体。 除了从永生化的B细胞群体分泌的单克隆抗体之外，多克隆抗体可以从本发明的转基因鸡放置的卵中获得。

公开(公告)号：US20060191026A1

公开(公告)日：2006-08-24

申请号：US11062325

申请日：2005-02-18

申请人： Lei Zhu ,  Wen Zhou ,  Robert Etches

发明人： Lei Zhu ,  Wen Zhou ,  Robert Etches

IPC分类号： A01K67/027 ,  C07K16/44

CPC分类号： A01K67/0275 ,  A01K67/0278 ,  A01K2217/052 ,  A01K2227/30 ,  A01K2267/01 ,  C07K16/02 ,  C07K2317/11 ,  C07K2317/732 ,  C07K2317/77 ,  C12N15/8509

摘要： Transgenes encoding exogenous antibodies are stably integrated into donor cells and are present in the somatic tissue of chimeric birds. The transgenes encode exogenous antibodies and are preferably expressed in the oviduct for collection in the egg. Tissue specificity is provided by selecting the content of the transgene accordingly. Birds whose genome is comprised of trangene-derived exogenous antibody-encoding DNA express exogenous antibodies having desirable chemical properties with increased therapeutic utility compared to antibodies derived from bacterial expression systems.

公开(公告)号：US20060053504A1

公开(公告)日：2006-03-09

申请号：US10524089

申请日：2003-08-11

申请人： Lei Zhu ,  Peggy Winters-Digiacinto ,  Robert Etches

发明人： Lei Zhu ,  Peggy Winters-Digiacinto ,  Robert Etches

IPC分类号： A01K67/027 ,  A61K35/54

CPC分类号： C12N15/8509 ,  A01K67/0275 ,  A01K67/0278 ,  A01K2207/15 ,  A01K2217/00 ,  A01K2217/05 ,  A01K2227/30 ,  A01K2267/01 ,  C07K16/02 ,  C07K16/44 ,  C07K2317/21 ,  C12N2830/008 ,  C12N2830/15 ,  C12N2830/42 ,  C12N2830/60 ,  C12N2830/90 ,  C12N2840/20 ,  C12N2840/203

摘要： Transgenes encoding exogenous proteins are stably integrated into embryonic stem cells and are present in the somatic tissue of transgenic or chimeric birds. The transgenes encode exogenous proteins and are expressed in any of endodermal, ectodermal, mesodermal, or extra embryonic tissue. Tissue specificity is provided by selecting the content of the transgene accordingly. Transgenic birds whose genome is comprised of trangene derived exogenous DNA express exogenous proteins with tissue specificity, and specifically express exogenous proteins in the tubular gland cells of the oviduct to concentrate exogenous proteins in egg white.

摘要翻译： 编码外源蛋白的转基因稳定整合到胚胎干细胞中，并存在于转基因或嵌合鸟类的体细胞组织中。 转基因编码外源蛋白，并在内胚层，外胚层，中胚层或额外的胚胎组织中表达。 通过相应地选择转基因的内容来提供组织特异性。 基因组由转基因外源DNA组成的转基因鸟类表达具有组织特异性的外源蛋白质，并特异性表达输卵管细胞中的外源蛋白质，以将外源蛋白质浓缩在蛋清中。

公开(公告)号：US20110277048A1

公开(公告)日：2011-11-10

申请号：US12835721

申请日：2010-07-13

申请人： Robert J. Etches ,  Robert M. Kay ,  Philip Leighton ,  Lei Zhu

发明人： Robert J. Etches ,  Robert M. Kay ,  Philip Leighton ,  Lei Zhu

IPC分类号： A01K67/027 ,  C12N5/10 ,  C12N5/16

CPC分类号： C07K16/461 ,  A01K67/0275 ,  A01K67/0278 ,  A01K2207/15 ,  A01K2217/00 ,  A01K2217/075 ,  A01K2227/30 ,  A01K2267/01 ,  C07K14/70503 ,  C07K16/02 ,  C07K2317/21 ,  C12N15/8509 ,  C12N2800/204 ,  C12N2800/30

摘要： Human antibodies are produced in transgenic chickens having functional genetic components of the human immune system stably integrated in the genome. Techniques are described to integrate unrearranged human heavy and light chain immunoglobulin loci into the genome of the transgenic chicken.The functional description of the endogenous immunoglobulin loci yields an animal whose antibody repertoire in response to antigen is fully human.In the preferred embodiment, the human immunoglobulin locus is at least as large as the native human locus and exhibits functional class switching to yield IgG isotype antibodies. In addition to monoclonal antibodies secreted from immortalized B cell populations, polyclonal antibodies may be obtained from eggs laid by the transgenic chickens of the invention.

摘要翻译： 在具有稳定整合在基因组中的人免疫系统的功能性遗传成分的转基因鸡中产生人抗体。 描述了将未经排列的人重链和轻链免疫球蛋白基因座整合到转基因鸡的基因组中的技术。 内源性免疫球蛋白基因座的功能描述产生一种抗体对抗原作出全部反应的动物。 在优选的实施方案中，人免疫球蛋白基因座至少与天然人基因座一样大，并显示功能类转换以产生IgG同种型抗体。 除了从永生化的B细胞群体分泌的单克隆抗体之外，多克隆抗体可以从本发明的转基因鸡放置的卵中获得。

公开(公告)号：US20180354957A1

公开(公告)日：2018-12-13

申请号：US16058299

申请日：2018-08-08

申请人： Konstantin Petrukhin ,  Christopher Cioffi ,  Graham Johnson ,  Rando Allikmets ,  Emily Freeman ,  Ping Chen ,  Michael Conlon ,  Lei Zhu

发明人： Konstantin Petrukhin ,  Christopher Cioffi ,  Graham Johnson ,  Rando Allikmets ,  Emily Freeman ,  Ping Chen ,  Michael Conlon ,  Lei Zhu

IPC分类号： C07D487/04 ,  C07D471/04

CPC分类号： C07D487/04 ,  C07D471/04

摘要： The present invention provides a compound having the structure: wherein R1, R2, R3, R4, and R5 are each independently H, halogen, CF3 or C1-C4 alkyl, wherein two or more of R1, R2, R3, R4, or R5 are other than H; R6 is H, OH, or halogen; and B is a substituted or unsubstituted heterobicycle, wherein when R1 is CF3, R2 is H, R3 is F, R4 is H, and R5 is H, or R1 is H, R2 is CF3, R3 is H, R4 is CF3, and R5 is H, or R1 is Cl, R2 is H, R3 is H, R4 is F, and R5 is H, or R1 is CF3, R2 is H, R3 is F, R4 is H, and R5 is H, or R1 is CF3, R2 is F, R3 is H, R4 is H, and R5 is H, or R1 is Cl, R2 is F, R3 is H, R4 is H, and R5 is H, then B is other than or a pharmaceutically acceptable salt thereof.

公开(公告)号：US20180222919A1

公开(公告)日：2018-08-09

申请号：US15944334

申请日：2018-04-03

申请人： Konstantin Petrukhin ,  Christopher Cioffi ,  Graham Johnson ,  Nicoleta Dobri ,  Emily Freeman ,  Ping Chen ,  Michael Conlon ,  Lei Zhu

发明人： Konstantin Petrukhin ,  Christopher Cioffi ,  Graham Johnson ,  Nicoleta Dobri ,  Emily Freeman ,  Ping Chen ,  Michael Conlon ,  Lei Zhu

IPC分类号： C07D498/04 ,  C07D213/79 ,  C07D213/80 ,  C07D231/56 ,  C07D237/24 ,  C07C233/56 ,  C07D249/04 ,  C07D471/04 ,  C07D487/04 ,  C07D491/052 ,  C07D239/28

CPC分类号： C07D498/04 ,  C07C233/56 ,  C07D213/79 ,  C07D213/80 ,  C07D231/56 ,  C07D237/24 ,  C07D239/28 ,  C07D249/04 ,  C07D471/04 ,  C07D487/04 ,  C07D491/052

摘要： The present invention provides a compound having the structure: (structurally represented) wherein R1, R2, R3, R4, and R5 are each independently H, halogen, CF3 or C1-C4 alkyl; R6 is alkyl; A is absent or present, and when present is —C(O)— or —C(O)NH—; B is substituted or unsubstituted monocycle, bicycle, heteromonocycle, heterobicycle, benzyl, CO2H or (C1-C4 alkyl)-CO2H, wherein when B is CO2H, then A is present and is —C(O)—, or a pharmaceutically acceptable salt thereof.

公开(公告)号：US08995609B2

公开(公告)日：2015-03-31

申请号：US13565337

申请日：2012-08-02

申请人： Lei Zhu ,  Tianye Niu

发明人： Lei Zhu ,  Tianye Niu

IPC分类号： A61B6/00 ,  G01N23/04 ,  G21K1/02 ,  A61B6/03

CPC分类号： G01N23/046 ,  A61B6/032 ,  A61B6/4078 ,  A61B6/4233 ,  A61B6/483 ,  A61B6/508 ,  G01N2223/419 ,  G01N2223/612 ,  G21K1/02 ,  G21K2207/005

摘要： Briefly described, in an exemplary form, the present invention discloses a system, method and apparatus for X-ray Compton scatter imaging. In one exemplary embodiment, the present invention uses two detectors in a volumetric CT system. A first detector is positioned generally in-line with the angle of attack of the incoming energy, or, generally in-line of path x, where x is the path of the incoming energy. The first, or primary, detector detects various forms of radiation emanating from an object undergoing testing. In some embodiments, the present invention further comprises a Compton scattering system positioned generally normal to path x. In some embodiments, the Compton scattering subsystem comprises a second detector and a pin-hole collimator. The second detector detects Compton scattering energy from the object being tested.

摘要翻译： 简要描述，在示例性形式中，本发明公开了一种用于X射线康普顿散射成像的系统，方法和装置。 在一个示例性实施例中，本发明在体积CT系统中使用两个检测器。 第一检测器通常与入射能量的迎角定位，或者通常沿着路径x排列，其中x是入射能量的路径。 第一或第一检测器检测从经历测试的对象发出的各种形式的辐射。 在一些实施例中，本发明还包括通常垂直于路径x定位的康普顿散射系统。 在一些实施例中，康普顿散射子系统包括第二检测器和针孔准直器。 第二个检测器检测来自被测物体的康普顿散射能量。

公开(公告)号：US08933225B2

公开(公告)日：2015-01-13

申请号：US12221399

申请日：2008-08-01

申请人： Ian Armitage ,  Eric L. Elliott ,  Marianne Langston ,  Steven P. Langston ,  Quentin J. McCubbin ,  Hirotake Mizutani ,  Matthew Stirling ,  Lei Zhu

发明人： Ian Armitage ,  Eric L. Elliott ,  Marianne Langston ,  Steven P. Langston ,  Quentin J. McCubbin ,  Hirotake Mizutani ,  Matthew Stirling ,  Lei Zhu

IPC分类号： C07D487/04 ,  C07C219/24 ,  C07D241/50 ,  C07D211/96 ,  C07D295/22 ,  C07C217/52 ,  C07D471/08 ,  C07C215/44 ,  C07D213/89 ,  C07D239/30 ,  C07C211/35

CPC分类号： C07D487/04 ,  C07C215/44 ,  C07C217/52 ,  C07C219/24 ,  C07C315/04 ,  C07C317/50 ,  C07C2601/08 ,  C07D211/96 ,  C07D213/89 ,  C07D241/50 ,  C07D295/22 ,  C07D305/08 ,  C07D471/08 ,  Y02P20/55

摘要： Disclosed are processes and synthetic intermediates for the synthesis of compounds of formula (V) wherein the variables have the definitions contained herein. Such compounds are E1 activating enzyme inhibitors, and are useful for the treatment of disorders of cell proliferation, particularly cancer, and other disorders associated with E1 activity.

摘要翻译： 公开了用于合成式（V）化合物的方法和合成中间体，其中变量具有本文所含的定义。 这些化合物是E1活化酶抑制剂，并且可用于治疗细胞增殖，特别是癌症和与E1活性相关的其它病症的病症。

公开(公告)号：US08809906B2

公开(公告)日：2014-08-19

申请号：US13613177

申请日：2012-09-13

申请人： Lei Zhu ,  Shigeaki Sekiguchi ,  Shinsuke Tanaka ,  Kenichi Kawaguchi

发明人： Lei Zhu ,  Shigeaki Sekiguchi ,  Shinsuke Tanaka ,  Kenichi Kawaguchi

IPC分类号： H01L31/102

CPC分类号： G02B6/122 ,  G02B6/29352 ,  G02B2006/121 ,  G02F1/025 ,  H01L31/105

摘要： A semiconductor optical device includes a first clad layer, a second clad layer and an optical waveguide layer sandwiched between the first clad layer and the second clad layer, wherein the optical waveguide layer includes a first semiconductor layer, a second semiconductor layer disposed on the first semiconductor layer and extending in one direction, and a third semiconductor layer covering a top surface of the second semiconductor layer, and wherein the first semiconductor layer includes an n-type region disposed on one side of the second semiconductor layer, a p-type region disposed on the other side of the second semiconductor layer, and an i-type region disposed between the n-type region and the p-type region, and wherein the second semiconductor layer has a band gap narrower than band gaps of the first semiconductor layer and the third semiconductor layer.

摘要翻译： 半导体光学器件包括第一覆盖层，第二覆盖层和夹在第一覆盖层和第二覆盖层之间的光波导层，其中，光波导层包括第一半导体层，第二半导体层，设置在第一覆盖层 半导体层并且沿一个方向延伸，以及覆盖第二半导体层的顶表面的第三半导体层，并且其中第一半导体层包括设置在第二半导体层一侧的n型区域，p型区域 设置在第二半导体层的另一侧，以及设置在n型区域和p型区域之间的i型区域，并且其中第二半导体层具有比第一半导体层的带隙窄的带隙 和第三半导体层。

公开(公告)号：US08705827B2

公开(公告)日：2014-04-22

申请号：US13088134

申请日：2011-04-15

申请人： Lei Zhu ,  Tianye Niu

发明人： Lei Zhu ,  Tianye Niu

IPC分类号： G06K9/00

CPC分类号： G06T5/002 ,  G06T5/50 ,  G06T11/005 ,  G06T2207/10081 ,  G06T2207/20224

摘要： Described herein are improved methods for correcting cone beam computed tomography signals to reduce scatter contamination contained therein. Generally, the improved methods involve generating a plurality of two-dimensional projection images of a subject from a three-dimensional multi-detector computed tomography image of the subject. This is followed by subtracting the plurality of two-dimensional projection images from a plurality of two-dimensional cone beam projection images of the subject to produce a plurality of two-dimensional estimated error projections that comprise an estimated error in the plurality of two-dimensional cone beam projection images. The plurality of two-dimensional estimated error projection images are subtracted from the plurality of two-dimensional cone beam projection images to generate a plurality of two-dimensional corrected cone beam projection images. A three-dimensional corrected cone beam computed tomography image of the subject is then constructed from the plurality of two-dimensional corrected cone beam projection images.

摘要翻译： 这里描述了用于校正锥形束计算机断层摄影信号以减少其中包含的散射污染的改进方法。 通常，改进的方法包括从受试者的三维多检测器计算机断层摄影图像生成受试者的多个二维投影图像。 随后从对象的多个二维锥形束投影图像中减去多个二维投影图像，以产生多个二维估计误差投影，其包括多个二维投影图像中的估计误差 锥形投影图像。 从多个二维锥形束投影图像中减去多个二维估计误差投影图像，以产生多个二维校正锥束投影图像。 然后从多个二维校正的锥形束投影图像中构建被摄体的三维校正锥束计算机断层摄影图像。