Expression of phytase in plants
    3.
    发明授权
    Expression of phytase in plants 失效
    植酸酶中植酸酶的表达

    公开(公告)号:US5593963A

    公开(公告)日:1997-01-14

    申请号:US146424

    申请日:1993-11-02

    摘要: The present invention provides for the expression of phytase in transgenic plants or plant organs and methods for the production of such plants. DNA expression constructs are provided for the transformation of plants with a gene encoding phytase under the control of regulatory sequences which are capable of directing the expression of phytase. These regulatory sequences include sequences capable of directing transcription in plants, either constitutively, or stage and/or tissue specific, depending on the use of the plant or parts thereof. The transgenic plants and plant organs provided by the present invention may be applied to a variety of industrial processes either directly, e.g. in animal feeds or alternatively, the expressed phytase may be extracted and if desired, purified before application.

    摘要翻译: 本发明提供了植酸酶在转基因植物或植物器官中的表达及其生产方法。 提供DNA表达构建体用于在能够指导植酸酶表达的调控序列的控制下用编码植酸酶的基因转化植物。 这些调节序列包括根据植物或其部分的用途能够在植物中指导转录的序列,组成型或阶段和/或组织特异性的序列。 本发明提供的转基因植物和植物器官可以直接应用于各种工业过程,例如, 在动物饲料中,或者,可以提取表达的肌醇六磷酸酶,如果需要,在施用前进行纯化。

    Process for the incorporation of foreign dna into the genome of
dicotyledonous plants
    6.
    发明授权
    Process for the incorporation of foreign dna into the genome of dicotyledonous plants 失效
    将外来dna掺入双子叶植物基因组的方法

    公开(公告)号:US4940838A

    公开(公告)日:1990-07-10

    申请号:US583022

    申请日:1984-02-23

    CPC分类号: C12N15/8205

    摘要: The invention relates to a process for the incorporation of foreign DNA into chromosomes of dicotyledonous plants by infecting the plants or incubating plant protoplasts with Agrobacterium bacteria, which contain one or more plasmids, wherein bacteria are used which contain at least one plasmid having the vir-region of a Ti (tumour inducing) plasmid but no T-region, and at least one other plasmid having a T-region with incorporated therein foreign DNA but no vir-region, as well as to a Agrobacterium bacteria wherein at least one plasmid which has the vir-region of a Ti (tumour inducing) plasmid but no T-region and at least one other plasmid which has a wild type T-region with incorporated in it foreign DNA but no vir-region.

    摘要翻译: 本发明涉及通过用含有一种或多种质粒的农杆菌细菌感染植物或培养植物原生质体将外来DNA掺入双子叶植物的染色体的方法,其中使用含有至少一种具有vir- Ti(肿瘤诱导)质粒但不含T区的区域,以及至少一种具有T区的外源DNA但不含有vir区的其他质粒,以及至​​少一种质粒, 具有Ti(肿瘤诱导)质粒的vir区域,但没有T区和至少一个具有野生型T区的其他质粒,其中掺入外源DNA但没有vir区。

    Process for the incorporation of foreign DNA into the genome of
dicotyledonous plants
    9.
    发明授权
    Process for the incorporation of foreign DNA into the genome of dicotyledonous plants 失效
    将外来DNA掺入双子叶植物基因组的方法

    公开(公告)号:US5464763A

    公开(公告)日:1995-11-07

    申请号:US173271

    申请日:1993-12-23

    CPC分类号: C12N15/8205

    摘要: The invention relates to a process for the incorporation of foreign DNA into chromosomes of dicotyledonous plants by infecting the plants or incubating plant protoplasts with Agrobacterium bacteria, which contain one or more plasmids, wherein bacteria are used which contain at least one plasmid having the vir-region of Ti (tumor inducing) plasmid but no T-region, and at least one other plasmid having a T-region with incorporated therein foreign DNA but no vir-region, as well as to a Agrobacterium bacteria, suitable for use in the process according to claim 1 wherein at least one plasmid which has the vir-region of a Ti (tumor inducing) plasmid but no T-region and at least one other plasmid which has a wild type T-region with incorporated in it foreign DNA but no vir-region.

    摘要翻译: 本发明涉及通过用含有一种或多种质粒的农杆菌细菌感染植物或培养植物原生质体将外来DNA掺入双子叶植物的染色体的方法,其中使用含有至少一种具有vir- Ti(肿瘤诱导)质粒的区域,但没有T区,以及至少一种其他具有T区的外源DNA但不含有vir区的质粒,以及适用于该方法的农杆菌 根据权利要求1的方法,其中至少一种具有Ti(诱导肿瘤)质粒但不具有T区的vir区的至少一种质粒和至少一种具有野生型T区的其它质粒,其中掺入外源DNA但不含 vir区域。

    Expression of phytase in plants
    10.
    发明授权
    Expression of phytase in plants 失效
    植酸酶中植酸酶的表达

    公开(公告)号:US6022846A

    公开(公告)日:2000-02-08

    申请号:US97847

    申请日:1998-06-15

    摘要: The present invention provides for the expression of phytase in transgenic plants or plant organs and methods for the production of such plants. DNA expression constructs are provided for the transformation of plants with a gene encoding phytase under the control of regulatory sequences which are capable of directing the expression of phytase. These regulatory sequences include sequences capable of directing transcription in plants, either constitutively, or stage and/or tissue specific, depending on the use of the plant or parts thereof. The transgenic plants and plant organs provided by the present invention may be applied to a variety of industrial processes either directly, e.g. in animal feeds or alternatively, the expressed phytase may be extracted and if desired, purified before application.

    摘要翻译: 本发明提供了植酸酶在转基因植物或植物器官中的表达及其生产方法。 提供DNA表达构建体用于在能够指导植酸酶表达的调控序列的控制下用编码植酸酶的基因转化植物。 这些调节序列包括根据植物或其部分的用途能够在植物中指导转录的序列,组成型或阶段和/或组织特异性的序列。 本发明提供的转基因植物和植物器官可以直接应用于各种工业过程,例如, 在动物饲料中,或者,可以提取表达的肌醇六磷酸酶,如果需要,在施用前进行纯化。