公开(公告)号：US20240302352A1

公开(公告)日：2024-09-12

申请号：US18182091

申请日：2023-03-10

Applicant: SHANDONG UNIVERSITY ,  YANSHAN UNIVERSITY

Inventor： Chuanzhen HUANG ,  Zhuang CHEN ,  Hanlian LIU ,  Peng YAO ,  Dun LIU ,  Hongtao ZHU ,  Bin ZOU ,  Zhen WANG ,  Jun WANG ,  Longhua XU ,  Shuiquan HUANG ,  Meina QU ,  Zhengkai XU ,  Minting WANG ,  Yabin GUAN

IPC: G01N33/50 ,  B33Y70/00 ,  B33Y80/00 ,  C12N5/079 ,  C12N5/0793 ,  C12N5/0797

CPC classification number: G01N33/5082 ,  B33Y70/00 ,  B33Y80/00 ,  C12N5/0618 ,  C12N5/0619 ,  C12N5/0623 ,  C12N2501/13 ,  C12N2513/00 ,  C12N2533/54 ,  C12N2533/74

Abstract: A method of rapid constructing human cerebral cortical organoids by 3D bioprinting and an application including preparing microfluidic chips, preparation of hydrogel of human cerebral cortical organoids, and printing of human cerebral cortical organoids. The microfluidic chip comprises a mixed-flow channel layer, liquid pool layer, microporous array layer, human cerebral cortical organoid culture layer, and culture medium recovery layer; the human cerebral cortical organoid hydrogel has gelatin, alginate, and hyaluronic acid; printing directly human cerebral cortical organoids in microfluidic chips by FRESH printing method, obtaining human cerebral cortical organoid chips after packaging. The application directly constructs large-scale human cerebral cortex-like with three layers of mutually connected structures in situ in organ chip through 3D bioprinting, simulates cerebrospinal fluid circulation through perfusion culture.

公开(公告)号：US20220105682A1

公开(公告)日：2022-04-07

申请号：US16479828

申请日：2018-10-17

Applicant: SHANDONG UNIVERSITY

Inventor： Chuanzhen HUANG ,  Zhen WANG ,  Jun WANG ,  Bin ZOU ,  Hanlian LIU ,  Hongtao ZHU ,  Peng YAO

IPC: B29C64/35 ,  B08B3/12 ,  B08B3/08 ,  B33Y40/20

Abstract: A self-rotation cleaning device has outer and inner housings, a workpiece rotating system, an ultrasonic cleaning system and a fluid perfusion system. The inner housing is in the outer housing in a horizontal direction. A cylindrical cavity is inside the inner housing. One end of the inner housing has a sealing cover detachably connected thereto, and the other end is closed. The workpiece rotating system is in the cavity for fixing a member to be cleaned, and realizes self-rotation of the member. The ultrasonic cleaning system supplies mechanical energy to the cleaning liquid in the inner housing to generate bubbles therein. The bubbles remove residual resin attached to the cleaned member surface by continuous vibration and burst. The fluid perfusion system provides self-rotation power for the cleaned member, and continuously delivers the cleaning liquid to the inside of the cleaned member, and the cleaning liquid is carried out after cleaning.

公开(公告)号：US20240216581A1

公开(公告)日：2024-07-04

申请号：US17911188

申请日：2021-09-17

Applicant: SHANDONG UNIVERSITY ,  YANSHAN UNIVERSITY

Inventor： Chuanzhen HUANG ,  Xu HAN ,  Hanlian LIU ,  Peng YAO ,  Hongtao ZHU ,  Bin ZOU ,  Jun WANG

IPC: A61L27/52 ,  A61L27/20 ,  A61L27/22 ,  B33Y10/00 ,  B33Y80/00 ,  C08J3/075

CPC classification number: A61L27/52 ,  A61L27/20 ,  A61L27/222 ,  B33Y10/00 ,  B33Y80/00 ,  C08J3/075 ,  C08J2305/04 ,  C08J2401/28 ,  C08J2405/00 ,  C08J2405/08

Abstract: A photocurable composite hydrogel matrix precursor, a preparation method thereof and a scaffold with same. The photocurable composite hydrogel matrix precursor includes gelatin methacrylate, sodium alginate, sodium carboxymethyl cellulose and chondroitin sulfate, where the mass ratio of a photoinitiator to the gelatin methacrylate to the sodium alginate to the sodium carboxymethyl cellulose to the chondroitin sulfate is (0.2-0.3):(8-10):(1-3):(0.6-0.8):(0.05-0.07). By adoption of the precursor, a cell-loaded printing hydrogel scaffold can be obtained through an extrusion-based 3D bio-printing technology, and the scaffold is controllable in form, good in moldability, high in precision, and has good stability; the biocompatibility and bioactivity are high, so that a good growing environment can be provided for fibroblasts; and the preparation process is simple and can be completed within a short time, moreover, the porosity and mechanical performance of the 3D printing hydrogel scaffold can be adjusted by adjusting the raw material ratio.

公开(公告)号：US20250002660A1

公开(公告)日：2025-01-02

申请号：US18692195

申请日：2022-01-11

Applicant: SHANDONG UNIVERSITY ,  YANSHAN UNIVERSITY

Inventor： Chuanzhen HUANG ,  Zhichao WANG ,  Hanlian LIU ,  Peng YAO ,  Wei WANG ,  Zhen WANG ,  Longhua XU ,  Shuiquan HUANG ,  Jun WANG ,  Hongtao ZHU ,  Bin ZOU

IPC: C08J3/075 ,  B33Y80/00 ,  C08J3/28 ,  C08J9/26 ,  C08J9/28 ,  C12N5/00

Abstract: A method for prepring a porous aerogel scaffold includes: adding a photoinitiator and polyethylene glycol diacrylate in a buffer solution, dissolving by heating and evenly mixing, adding Pluronic F127 into the mixed solution, and standing at a low temperature to obtain an aerogel scaffold material; printing a hydrogel scaffold by using a 3D printing technology, and performing UV irradiation so that a cross-linking of the hydrogel scaffold is caused to form a three-dimensional scaffold with a stable structure, performing low-temperature soaking to remove Pluronic F127, and then freeze drying the three-dimensional scaffold to obtain the porous aerogel scaffold. Wherein, Pluronic F127 serves as a sacrificial material which is removed after the 3D printing of the hydrogel scaffold is completed, and then a porous structure can be formed in the scaffold in combination with a freeze drying technology, which facilitates the survival, growth and proliferation of cells during the three-dimensional culture.

公开(公告)号：US20240108784A1

公开(公告)日：2024-04-04

申请号：US18081964

申请日：2022-12-15

Applicant: SHANDONG UNIVERSITY ,  YANSHAN UNIVERSITY

Inventor： Chuanzhen HUANG ,  Zhuang CHEN ,  Hanlian LIU ,  Peng YAO ,  Zhenyu SHI ,  Dun LIU ,  Hongtao ZHU ,  Bin ZOU ,  Zhen WANG ,  Minting WANG ,  Longhua XU ,  Shuiquan HUANG ,  Meina QU ,  Zhengkai XU ,  Yabin GUAN

IPC: A61L26/00 ,  B29C64/118 ,  B33Y10/00 ,  B33Y70/00 ,  B33Y80/00

CPC classification number: A61L26/008 ,  A61L26/0052 ,  B29C64/118 ,  B33Y10/00 ,  B33Y70/00 ,  B33Y80/00 ,  B29K2005/00 ,  B29L2031/753

Abstract: A hydrogel for cell-laden bioprinting, bioink, and a preparation method and an application thereof, relates to the technical field of biomedical polymer hydrogels. The hydrogel for cell-laden bioprinting is polymer gel formed by adding a cell-specific material into a matrix of alginate and gelatin and crosslinking and curing, wherein the cell-specific material is polypeptide selected according to different laden cells. The structures printed using the hydrogel may have the advantages such as adjustable mechanical properties, adjustable porosity, high biocompatibility, high printing accuracy, and high customizability, which may widely support the printing of human tissues and organs such as spinal cord, cartilage, and heart, and has good prospects for applications in tissue repair, organ transplantation and so on.

公开(公告)号：US20230405874A1

公开(公告)日：2023-12-21

申请号：US17941726

申请日：2022-09-09

Applicant: SHANDONG UNIVERSITY ,  YANSHAN UNIVERSITY

Inventor： Chuanzhen HUANG ,  Xuefei LIU ,  Hanlian LIU ,  Peng YAO ,  Hongtao ZHU ,  Bin ZOU ,  Dun LIU ,  Jun WANG ,  Zhen WANG ,  Longhua XU ,  Shuiquan HUANG

IPC: B28D5/04 ,  G02B1/118 ,  G02B1/113 ,  B23K26/0622

CPC classification number: B28D5/04 ,  G02B1/118 ,  G02B1/113 ,  B23K26/0624 ,  B23K2103/56

Abstract: A monocrystalline silicon micro-nano dual-scale anti-reflection texture and a preparation method therefor. The preparation method combines nanosecond-laser-assisted waterjet near-damage-free processing and femtosecond laser scanning, and subsurface damage caused by a re-cast layer phenomenon and a hot crack in a monocrystalline silicon laser texturing process can be effectively reduced by combining a nanosecond-laser-assisted waterjet near-damage-free processing technology and an ultra-short pulse femtosecond laser cold processing technology; and meanwhile, a micro-scale frame structure and a nano-scale structure can be flexibly modified respectively by adjusting nanosecond-laser-assisted waterjet technological parameters and femtosecond laser technological parameters, a geometry light trapping effect and an effective dielectric effect can be achieved in a micro-nano dual-scale hybrid structure at the same time, and surface reflection is reduced.

公开(公告)号：US20230219856A1

公开(公告)日：2023-07-13

申请号：US17706045

申请日：2022-03-28

Applicant: SHANDONG UNIVERSITY ,  YANSHAN UNIVERSITY

Inventor： Chuanzhen HUANG ,  Yan ZHANG ,  Hanlian LIU ,  Zhenyu SHI ,  Peng YAO ,  Zhen WANG ,  Longhua XU ,  Shuiquan HUANG ,  Jun WANG ,  Hongtao ZHU ,  Bin ZOU

IPC: C04B35/58 ,  C04B35/626 ,  C04B35/645 ,  B23B27/14

CPC classification number: C04B35/58 ,  C04B35/6261 ,  C04B35/6264 ,  C04B35/645 ,  B23B27/148 ,  C04B2235/3856 ,  C04B2235/3886 ,  C04B2235/404 ,  C04B2235/405 ,  C04B2235/6581 ,  C04B2235/6562 ,  C04B2235/6567 ,  B23B2226/18

Abstract: The present invention relates to the field of new materials technology, in particular to carbon nitride composite ceramic tool materials, preparation method and cutting tools thereof. The raw materials comprise carbon nitride, titanium carbonitride, molybdenum, nickel and cobalt, carbon nitride as the matrix phase, titanium carbonitride as the reinforcing phase are added to the carbon nitride based composite ceramic materials, with molybdenum, nickel and cobalt as a suitable sintering aid, dense composite tool material is obtained with vacuum hot press sintering method. The prepared carbon nitride based composite ceramic tool materials boast the advantages of low cost, high hardness, high bending strength and high fracture toughness, which is an important way to promote the innovation, development and popularization of carbon nitride materials.

公开(公告)号：US20220373444A1

公开(公告)日：2022-11-24

申请号：US17619092

申请日：2021-01-21

Applicant: SHANDONG UNIVERSITY

Inventor： Chuanzhen HUANG ,  Zhengyi TANG ,  Hanlian LIU ,  Hongtao ZHU ,  Bin ZOU ,  Peng YAO ,  Jun WANG

IPC: G01N3/24

Abstract: A method and system for calculating a primary shear zone stored energy field during steady-state cutting, the method including: fitting parameters of a workpiece material stored energy evolution model; discretizing the primary shear zone into infinitesimals on a main shear plane. The infinitesimals are small enough, a strain, strain rate, and temperature are assumed constant; introducing an equivalent cutting edge model simplifying three-dimensional cutting into two-dimensional cutting, calculating element strain and strain rate using a shear plane model, and analyzing element temperature using a heat conduction equation; deriving a differential equation of stored energy versus location in the primary shear zone using stored energy evolution, strain rate distribution, strain distribution, and temperature distribution models; and solving the differential equation for each infinitesimal using an initial shear plane as a model boundary, obtaining stored energy at each location to obtain a stored energy field distribution of the primary shear zone.

公开(公告)号：US20240067567A1

公开(公告)日：2024-02-29

申请号：US17955237

申请日：2022-09-28

Applicant: SHANDONG UNIVERSITY ,  YANSHAN UNIVERSITY

Inventor： Chuanzhen HUANG ,  Zexin LI ,  Hanlian LIU ,  Zhenyu SHI ,  Peng YAO ,  Dun LIU ,  Hongtao ZHU ,  Bin ZOU ,  Jun WANG ,  Zhen WANG ,  Longhua XU ,  Shuiquan HUANG ,  Meina QU ,  Zhengkai XU

IPC: C04B35/117 ,  B23B27/14 ,  C04B35/626 ,  C04B35/645

CPC classification number: C04B35/117 ,  B23B27/148 ,  C04B35/6261 ,  C04B35/6264 ,  C04B35/62655 ,  C04B35/645 ,  C04B2235/3206 ,  C04B2235/3217 ,  C04B2235/3224 ,  C04B2235/3258 ,  C04B2235/3856 ,  C04B2235/3886 ,  C04B2235/606 ,  C04B2235/656 ,  C04B2235/6581 ,  C04B2235/9607

Abstract: An alumina-based ceramic tool material with low thermal expansion and a preparation process thereof, accordingly, the ceramic tool material may have both the high hardness of alumina ceramics after the hot pressing sintering, and reduces the thermal expansion coefficient of the overall ceramic material by adding the Sc2W3O12 as the negative thermal expansion phase, which improves the thermal shock resistance of ceramic tools in high-speed cutting engineering and meets the requirements of large temperature range during the machining of nickel-based superalloys. Moreover, the composite material does not use metal binder and has strong thermal stability even in the high-speed machining under extreme heat-force-chemistry coupling, so it has a high machining compatibility for nickel-based superalloys.

公开(公告)号：US20230405187A1

公开(公告)日：2023-12-21

申请号：US17939524

申请日：2022-09-07

Applicant: YANSHAN UNIVERSITY ,  SHANDONG UNIVERSITY

Inventor： Chuanzhen HUANG ,  Xu HAN ,  Hanlian LIU ,  Zhenyu SHI ,  Peng YAO ,  Hongtao ZHU ,  Bin ZOU ,  Dun LIU ,  Zhen WANG ,  Longhua XU ,  Shuiquan HUANG

IPC: A61L27/52 ,  C08J3/075 ,  C12N5/00 ,  A61L27/26 ,  B33Y70/00 ,  B33Y80/00 ,  B33Y40/00 ,  B33Y10/00

CPC classification number: A61L27/52 ,  C08J3/075 ,  C12N5/0062 ,  A61L27/26 ,  B33Y70/00 ,  B33Y80/00 ,  B33Y40/00 ,  B33Y10/00 ,  C08J2489/04 ,  C08J2301/28 ,  C12N2513/00 ,  C12N2533/54 ,  C12N2533/78

Abstract: A composite hydrogel for light-cured 3D cell-laden printing and a preparation method and application thereof. The composite hydrogel of the present disclosure combines advantages of gelatin methacryloyl, sodium carboxymethylcellulose, hyaluronic acid-glutamic acid polymer, and the like. The provided composite hydrogel for 3D printing has the characteristics of low toxicity, good biocompatibility and adjustable mechanical properties, can provide cells with a three-dimensional living environment, promotes cell adhesion and migration on gradient scaffolds, and is suitable for tissue engineering scaffolds and cell-laden printing of tissues. The printing process of a scaffold is simple and can be completed within a short time, and the porosity and mechanical properties of the 3D printed hydrogel scaffold can be adjusted by adjusting the proportion of HA-Glu and Col in the hydrogel system.