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    Method for screening of a lipase having improved enzymatic activity using yeast surface display vector and the lipase
    6.
    发明申请
    Method for screening of a lipase having improved enzymatic activity using yeast surface display vector and the lipase 失效
    使用酵母表面显示载体和脂肪酶筛选具有改善的酶活性的脂肪酶的方法

    公开(公告)号：US20060275760A1

    公开(公告)日：2006-12-07

    申请号：US10527438

    申请日：2003-09-04

    申请人： Eui-Sung Choi ,  Jung-Hoon Sohn ,  So-Young Kim

    发明人： Eui-Sung Choi ,  Jung-Hoon Sohn ,  So-Young Kim

    IPC分类号： C40B30/06 ,  C40B40/08

    CPC分类号： C40B40/02 ,  C12N9/18 ,  C12N15/1037

    摘要： The present invention relates to a method for screening of the lipase having improved enzymatic activity using yeast surface display vector and the mutant lipase prepared by the same, more particularly to the method comprising; 1) cloning lipase gene into surface display vector, 2) preparing mutant lipase gene library of the step 1 by mutagenic PCR, 3) transforming the mutant lipase gene library of the step 2 and surface display vector into host cell, and 4) measuring the activity of the mutant lipase displayed in the surface of the transformed host cell and selecting the mutant lipase prepared by the same. The method of the present invention can screen the lipase having improved enzymatic activity. So, it can effectively be used for the various fields, such as food and detergent industry.

    摘要翻译： 本发明涉及使用酵母表面显示载体筛选具有改善的酶活性的脂肪酶的方法和由其制备的突变脂肪酶，更具体地涉及包括以下的方法： 1）将脂肪酶基因克隆到表面展示载体中，2）通过诱变PCR制备步骤1的突变脂肪酶基因文库，3）将步骤2的突变体脂肪酶基因文库和表面显示载体转化到宿主细胞中，4）测量 突变脂肪酶的活性显示在转化的宿主细胞的表面，并选择由其制备的突变体脂肪酶。 本发明的方法可以筛选具有改善的酶活性的脂肪酶。 因此，它可以有效地用于食品和洗涤剂行业等各个领域。

    Cell wall anchor proteins derived from yeast, genes thereof and cell surface expression systems using the same
    7.
    发明授权
    Cell wall anchor proteins derived from yeast, genes thereof and cell surface expression systems using the same 有权
    衍生自酵母的细胞壁锚蛋白，其基因和使用其的细胞表面表达系统

    公开(公告)号：US07132273B1

    公开(公告)日：2006-11-07

    申请号：US10333951

    申请日：2000-07-27

    申请人： Eui-Sung Choi ,  Jung-Hoon Sohn ,  So-Young Kim

    发明人： Eui-Sung Choi ,  Jung-Hoon Sohn ,  So-Young Kim

    IPC分类号： C12N1/20 ,  C12N15/00 ,  C12N1/14 ,  C12P21/04 ,  C12Q1/00 ,  C07K1/00 ,  C07H21/04 ,  C12Q1/68

    CPC分类号： C07K14/39

    摘要： The present invention provides novel cell wall anchor proteins derived from yeast, genes thereof and the genetic method for tethering polypeptide to the yeast cell wall using the same. Particularly, the present invention provides the novel GPI (glycosyl phosphatidyl inositol)-anchor protein genes, SFD1, GAS1, TIP1 and CWP1, and their proteins, PIR2 cell wall protein gene and its protein derived from Hansenula polymorpha, and the cell surface expression system using them which immobilize foreign enzymes or polypeptides on the cell wall of a microbial cell. In addition, the present invention provides the cell surface expression system using WSC1 gene and its protein derived from yeasts, including Hansenula polymorpha and Saccharomyces cerevisiae, and STA1 gene and its protein derived from Saccharomyces diastaticus. The cell surface expression system of the present invention expects an immobilization effect as biocatalysts by adhereing a desired protein to the cell surface, and provides a means of altering target protein characteristics such as binding affinity and stability by library screening.

    摘要翻译： 本发明提供了源自酵母的新型细胞壁锚蛋白，其基因，以及使用该酵母细胞壁将酵母细胞壁连接多肽的遗传方法。 特别地，本发明提供新型GPI（糖基磷脂酰肌醇） - 锚定蛋白基因，SFD1，GAS1，TIP1和CWP1及其蛋白质，PIR2细胞壁蛋白基因及其衍生自多形汉逊酵母的蛋白质，以及细胞表面表达系统 使用它们将外来酶或多肽固定在微生物细胞的细胞壁上。 此外，本发明提供了使用WSC1基因及其衍生自酵母的蛋白质，包括多形汉逊酵母和酿酒酵母的细胞表面表达系统，以及来源于糖酵母的STA1基因及其蛋白质。 本发明的细胞表面表达系统期望通过将期望的蛋白质粘附到细胞表面作为生物催化剂的固定效果，并且通过文库筛选提供改变靶蛋白特征如结合亲和力和稳定性的方法。

    Method for screening of a lipase having improved enzymatic activity using yeast surface display vector and the lipase
    8.
    发明授权
    Method for screening of a lipase having improved enzymatic activity using yeast surface display vector and the lipase 失效
    使用酵母表面显示载体和脂肪酶筛选具有改善的酶活性的脂肪酶的方法

    公开(公告)号：US07736643B2

    公开(公告)日：2010-06-15

    申请号：US10527438

    申请日：2003-09-04

    申请人： Eui-Sung Choi ,  Jung-Hoon Sohn ,  So-Young Kim

    发明人： Eui-Sung Choi ,  Jung-Hoon Sohn ,  So-Young Kim

    IPC分类号： A61K38/46 ,  C12N9/20 ,  C12N15/63 ,  C12N1/15 ,  C07K14/00 ,  C07H21/02

    CPC分类号： C40B40/02 ,  C12N9/18 ,  C12N15/1037

    摘要： The present invention relates to a method for screening of the lipase having improved enzymatic activity using yeast surface display vector and the mutant lipase prepared by the same, more particularly to the method comprising; 1) cloning lipase gene into surface display vector, 2) preparing mutant lipase gene library of the step 1 by mutagenic PCR, 3) transforming the mutant lipase gene library of the step 2 and surface display vector into host cell, and 4) measuring the activity of the mutant lipase displayed in the surface of the transformed host cell and selecting the mutant lipase prepared by the same. The method of the present invention can screen the lipase having improved enzymatic activity. So, it can effectively be used for the various fields, such as food and detergent industry.

    摘要翻译： 本发明涉及使用酵母表面显示载体筛选具有改善的酶活性的脂肪酶的方法和由其制备的突变脂肪酶，更具体地涉及包括以下的方法： 1）将脂肪酶基因克隆到表面展示载体中，2）通过诱变PCR制备步骤1的突变脂肪酶基因文库，3）将步骤2的突变体脂肪酶基因文库和表面显示载体转化到宿主细胞中，4）测量 突变脂肪酶的活性显示在转化的宿主细胞的表面，并选择由其制备的突变体脂肪酶。 本发明的方法可以筛选具有改善的酶活性的脂肪酶。 因此，它可以有效地用于食品和洗涤剂行业等各个领域。