公开(公告)号：US6054125A

公开(公告)日：2000-04-25

申请号：US9835

申请日：1998-01-20

申请人： Satoru Oka ,  Kazuhisa Ono ,  Seiko Shigeta ,  Takeshi Wada

发明人： Satoru Oka ,  Kazuhisa Ono ,  Seiko Shigeta ,  Takeshi Wada

IPC分类号： A61K39/35 ,  A61K38/00 ,  A61K49/00 ,  A61P37/08 ,  C07K14/00 ,  C07K14/435 ,  G01N33/48 ,  G01N33/53 ,  A61K39/00 ,  A61K39/36 ,  A61K39/38

CPC分类号： C07K14/43531 ,  A61K38/00

摘要： The present invention is directed to novel purified and isolated mite allergens possessing mite allergen activity with a molecular weight of about 94,000, about 40,000, about 16,000 or about 14,000 as determined by SDS-PAGE, which can be isolated from extracts of mites, and to a process for producing such mites allergens. The purified and isolated mite allergens of the present invention are useful as a pharmaceutical and a diagnostic composition for mite allergic diseases.

摘要翻译： 本发明涉及具有螨过敏原活性的新型纯化和分离的螨变应原，其分子量约为94,000，约40,000，约16,000或约14,000，通过SDS-PAGE测定，其可以从螨的提取物中分离，并且 一种生产这种螨变应原的方法。 本发明的纯化和分离的螨过敏原可用作螨过敏性疾病的药物和诊断组合物。

公开(公告)号：US5405758A

公开(公告)日：1995-04-11

申请号：US166818

申请日：1993-12-15

申请人： Satoru Oka ,  Kazuhisa Ono ,  Seiko Shigeta ,  Takeshi Wada

发明人： Satoru Oka ,  Kazuhisa Ono ,  Seiko Shigeta ,  Takeshi Wada

IPC分类号： A61B10/00 ,  A61K38/00 ,  A61K39/35 ,  A61K49/00 ,  A61P11/00 ,  A61P37/08 ,  C07K1/22 ,  C07K14/00 ,  C07K14/435 ,  C07K19/00 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N15/09 ,  C12N15/12 ,  C12N15/29 ,  C12P21/02 ,  C12R1/19 ,  G01N33/50 ,  G01N33/53 ,  A61K39/36 ,  C12N15/62

CPC分类号： C07K14/43531 ,  A61K38/00 ,  C07K2319/00 ,  C07K2319/40 ,  C07K2319/61 ,  Y10S530/858

摘要： The present invention is directed to a recombinant mite allergen obtainable by expression of a mite-body-derived gene, a gene which codes for said allergen, a mite allergen fragment, a polypeptide having an epitope contained in said allergen, an expression vector capable of expressing the gene, a bacterium, yeast or mammalian cell transformed with said expression vector, a method for producing said allergen, and a pharmaceutical composition or a diagnostic reagent for the treatment of mite allergic diseases.

摘要翻译： 本发明涉及通过表达螨体衍生基因，编码所述变应原的基因，螨变应原片段，具有所述变应原中包含的表位的多肽获得的重组螨变应原，能够 表达基因，用所述表达载体转化的细菌，酵母或哺乳动物细胞，生产所述变应原的方法，以及用于治疗螨过敏性疾病的药物组合物或诊断试剂。

公开(公告)号：US5314991A

公开(公告)日：1994-05-24

申请号：US748783

申请日：1991-08-22

申请人： Satoru Oka ,  Kazuhisa Ono ,  Seiko Shigeta ,  Takeshi Wada

发明人： Satoru Oka ,  Kazuhisa Ono ,  Seiko Shigeta ,  Takeshi Wada

IPC分类号： A61B10/00 ,  A61K38/00 ,  A61K39/35 ,  A61K49/00 ,  A61P11/00 ,  A61P37/08 ,  C07K1/22 ,  C07K14/00 ,  C07K14/435 ,  C07K19/00 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N15/09 ,  C12N15/12 ,  C12N15/29 ,  C12P21/02 ,  C12R1/19 ,  G01N33/50 ,  G01N33/53 ,  C07K15/08 ,  C07K15/00 ,  C07K15/12

CPC分类号： C07K14/43531 ,  A61K38/00 ,  C07K2319/00 ,  C07K2319/40 ,  C07K2319/61 ,  Y10S530/858

摘要： The present invention is directed to a recombinant mite allergen obtainable by expression of a mite-body-derived gene, a gene which codes for said allergen, a mite allergen fragment, a polypeptide having an epitope contained in said allergen, an expression vector capable of expressing the gene, a bacterium, yeast or mammalian cell transformed with said expression vector, a method for producing said allergen, and a pharmaceutical composition or a diagnostic reagent for the treatment of mite allergic diseases.

摘要翻译： 本发明涉及通过表达螨体衍生基因，编码所述变应原的基因，螨变应原片段，具有所述变应原中包含的表位的多肽获得的重组螨变应原，能够 表达基因，用所述表达载体转化的细菌，酵母或哺乳动物细胞，生产所述变应原的方法，以及用于治疗螨过敏性疾病的药物组合物或诊断试剂。

公开(公告)号：US5747047A

公开(公告)日：1998-05-05

申请号：US293605

申请日：1994-08-22

申请人： Satoru Oka ,  Kazuhisa Ono ,  Seiko Shigeta ,  Takeshi Wada

发明人： Satoru Oka ,  Kazuhisa Ono ,  Seiko Shigeta ,  Takeshi Wada

IPC分类号： A61K39/35 ,  A61K38/00 ,  A61K49/00 ,  A61P37/08 ,  C07K14/00 ,  C07K14/435 ,  G01N33/48 ,  G01N33/53 ,  A61K39/36 ,  C07K1/00 ,  C07K16/00

CPC分类号： C07K14/43531 ,  A61K38/00

摘要： The present invention is directed to novel purified and isolated mite allergens possessing mite allergen activity with a molecular weight of about 94,000, about 40,000, about 16,000 or about 14,000 as determined by SDS-PAGE, which can be isolated from extracts of mite, and to a process for producing such mite allergens. The purified and isolated mites allergens of the present invention are useful as a pharmaceutical and a diagnostic composition for mite allergic diseases.

摘要翻译： 本发明涉及具有螨过敏原活性的新型纯化和分离的螨变应原，其分子量约为94,000，约40,000，约16,000或约14,000，通过SDS-PAGE测定，其可以从螨的提取物中分离，并且 一种生产这种螨变应原的方法。 本发明的纯化和分离的螨过敏原可用作螨过敏性疾病的药物和诊断组合物。

公开(公告)号：US5773010A

公开(公告)日：1998-06-30

申请号：US564395

申请日：1995-11-29

申请人： Satoru Oka ,  Kazuhisa Ono ,  Seiko Shigeta ,  Takeshi Wada

发明人： Satoru Oka ,  Kazuhisa Ono ,  Seiko Shigeta ,  Takeshi Wada

IPC分类号： A61K39/35 ,  A61K38/00 ,  A61K49/00 ,  A61P37/08 ,  C07K1/14 ,  C07K14/00 ,  C07K14/435 ,  G01N33/53 ,  A61B8/00 ,  C12Q1/00

CPC分类号： C07K14/43531 ,  A61K38/00

摘要： New purified mite allergens useful as pharmaceutical and diagnostic compositions for the treatment and diagnosis of mite allergic diseases are provided. Both allergens are contained in fecal extracts of mites in culture. The high molecular weight allergen has a weight average molecular weight of 70,000 to 80,000 as determined by sedimentation equilibrium. This is a glycoprotein containing more than about 70% sugar, and possesses allergen activity. The low molecular weight antigen has a molecular weight of 1,500 to 5,000 as determined by Sephadex G25 gel filtration. This is a glycoprotein containing more than about 40% sugar, and also possesses allergen activity.

摘要翻译： 提供了用作治疗和诊断螨过敏性疾病的药物和诊断组合物的新的纯化螨过敏原。 两种过敏原都包含在文化中螨的粪便提取物中。 高分子量变应原具有通过沉降平衡测定的重均分子量为70,000至80,000。 这是含有超过约70％糖的糖蛋白，具有过敏原活性。 通过Sephadex G25凝胶过滤测定，低分子量抗原的分子量为1,500至5,000。 这是一种含有超过约40％糖的糖蛋白，也具有过敏原活性。

公开(公告)号：US5496554A

公开(公告)日：1996-03-05

申请号：US283905

申请日：1994-08-04

申请人： Satoru Oka ,  Kazuhisa Ono ,  Seiko Shigeta ,  Takeshi Wada

发明人： Satoru Oka ,  Kazuhisa Ono ,  Seiko Shigeta ,  Takeshi Wada

IPC分类号： A61K39/35 ,  A61K38/00 ,  A61K49/00 ,  A61P37/08 ,  C07K1/14 ,  C07K14/00 ,  C07K14/435 ,  G01N33/53

CPC分类号： C07K14/43531 ,  A61K38/00

摘要： New purified mite allergens useful as pharmaceutical and diagnostic compositions for the treatment and diagnosis of mite allergic diseases are provided. Both allergens are contained in fecal extracts of mites in culture. The high molecular weight allergen has a weight average molecular weight of 70,000 to 80,000 as determined by sedimentation equilibrium. This is a glycoprotein containing more than about 70% sugar, and possesses allergen activity. The low molecular weight antigen has a molecular weight of 1,500 to 5,000 as determined by Sephadex G25 gel filtration. This is a glycoprotein containing more than about 40% sugar, and also possesses allergen activity.

摘要翻译： 提供了用作治疗和诊断螨过敏性疾病的药物和诊断组合物的新的纯化螨过敏原。 两种过敏原都包含在文化中螨的粪便提取物中。 高分子量变应原具有通过沉降平衡测定的重均分子量为70,000至80,000。 这是含有超过约70％糖的糖蛋白，具有过敏原活性。 通过Sephadex G25凝胶过滤测定，低分子量抗原的分子量为1,500至5,000。 这是一种含有超过约40％糖的糖蛋白，也具有过敏原活性。

公开(公告)号：US08420326B2

公开(公告)日：2013-04-16

申请号：US10545239

申请日：2004-02-13

申请人： Kazuo Nishikawa ,  Hideo Nojima ,  Tetsuya Yoneda ,  Kazuhisa Ono ,  Seiko Shigeta ,  Masatoshi Oshita

发明人： Kazuo Nishikawa ,  Hideo Nojima ,  Tetsuya Yoneda ,  Kazuhisa Ono ,  Seiko Shigeta ,  Masatoshi Oshita

IPC分类号： G01N33/53

CPC分类号： G01N33/53

摘要： A method of evaluating performance of an activation gas deactivating an antigenic substance including the steps of causing the antigenic substance and the activation gas to react with each other, to obtain a processed antigenic substance, and causing an antibody against the antigenic substance with the processed antigenic substance to measure binding activity of the processed antigenic substance with the antibody is provided, whereby an evaluation method that can accurately and easily evaluate performance of an activation gas deactivating an antigenic substance is provided.

摘要翻译： 一种评估活化气体的活性气体的性能的方法，包括使所述抗原物质和活化气体彼此反应的步骤，以获得加工过的抗原物质，并使所述抗原物质的抗体与经处理的抗原性 提供了测定加工抗原物质与抗体的结合活性的物质，由此提供了能够准确且容易地评价使抗原性物质失活的活化气体的性能的评价方法。

公开(公告)号：US08263004B2

公开(公告)日：2012-09-11

申请号：US10545429

申请日：2004-02-13

申请人： Kazuo Nishikawa ,  Hideo Nojima ,  Tetsuya Yoneda ,  Kazuhisa Ono ,  Seiko Shigeta ,  Masatoshi Oshita

发明人： Kazuo Nishikawa ,  Hideo Nojima ,  Tetsuya Yoneda ,  Kazuhisa Ono ,  Seiko Shigeta ,  Masatoshi Oshita

IPC分类号： A61L2/00 ,  A61L101/00

CPC分类号： A61L2/0011 ,  A61L2/14 ,  A61L9/22

摘要： The present invention relates to a method of deactivating an antigenic substance by causing positive and negative ions to act on the antigenic substance, and the positive and negative ions are caused to act in an atmosphere in which each of positive ion concentration and negative ion concentration is at least about 50,000/cm3, and more preferably, at least about 100,000/cm3.

摘要翻译： 本发明涉及通过使正离子和负离子作用于抗原性物质而使抗原性物质失活的方法，使正离子和负离子在其中正离子浓度和负离子浓度分别为 至少约50,000 / cm3，更优选至少约100,000 / cm3。

公开(公告)号：US20100196384A1

公开(公告)日：2010-08-05

申请号：US12762649

申请日：2010-04-19

申请人： Takeshi Goto ,  Shuji Sato ,  Kazuhisa Ono ,  Seiko Shigeta ,  Seiji Kawamoto ,  Shigeru Goto

发明人： Takeshi Goto ,  Shuji Sato ,  Kazuhisa Ono ,  Seiko Shigeta ,  Seiji Kawamoto ,  Shigeru Goto

IPC分类号： A61K39/395 ,  C07K16/18 ,  C12N5/07 ,  G01N33/53 ,  A61P37/06

CPC分类号： C07K16/18 ,  C07K7/08 ,  C07K14/47 ,  Y10S530/808 ,  Y10S530/809

摘要： The present invention discloses anti-histone H1 monoclonal antibodies, hybridomas for the production thereof, and polypeptides, which are useful for suppressing, predicting, or diagnosing transplant rejection in organ transplantation.

摘要翻译： 本发明公开了用于抑制，预测或诊断器官移植移植排斥反应的抗组蛋白H1单克隆抗体，其生产用杂交瘤和多肽。

公开(公告)号：US06958229B2

公开(公告)日：2005-10-25

申请号：US09389318

申请日：1999-09-03

申请人： Osamu Suzuki ,  Kazuhisa Ono ,  Seiko Shigeta ,  Tsunehiro Aki ,  Kengo Akimoto

发明人： Osamu Suzuki ,  Kazuhisa Ono ,  Seiko Shigeta ,  Tsunehiro Aki ,  Kengo Akimoto

IPC分类号： A23K1/00 ,  A23K1/16 ,  C12P7/64

CPC分类号： C12P7/6427 ,  A23K10/16 ,  A23K20/158 ,  C12P7/6472

摘要： A method for producing highly unsaturated fatty acids comprising culturing a microorganism, belonging to the genus Mortierella and having resistance to a carbon source, in a medium having a carbon source concentration of at least 4% by weight, and collecting highly unsaturated fatty acids from the cultured products. Culturing the microorganism for about a week gives at least about 7 g/L of highly unsaturated fatty acids.

摘要翻译： 一种生产高度不饱和脂肪酸的方法，包括在碳源浓度至少为4％（重量）的培养基中培养属于被孢霉属（Mortierella）的微生物并具有耐碳源性的微生物，并从 培养产品。 将微生物培养约一周，得到至少约7g / L的高度不饱和脂肪酸。