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公开(公告)号：US09863939B2
公开(公告)日：2018-01-09
申请号：US12234506
申请日：2008-09-19
IPC分类号： G01N33/00 , G01N33/543 , G01N27/00
CPC分类号： G01N33/54326 , Y10T436/143333
摘要： Methods for analyte detection with magnetic sensors are provided. Aspects of the methods include producing a magnetic sensor device having a magnetically labeled analyte from a sample, such as a serum sample, bound to a surface of a magnetic sensor thereof; and obtaining a signal, e.g., a real-time signal, from the magnetic sensor to determine whether the analyte is present in the sample. Also provided are devices, systems and kits that find use in practicing the methods of the invention. The methods, devices, systems and kits of the invention find use in a variety of different applications, including detection of biomarkers, such as disease markers.
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公开(公告)号：US20090104707A1
公开(公告)日：2009-04-23
申请号：US12234506
申请日：2008-09-19
CPC分类号： G01N33/54326 , Y10T436/143333
摘要： Methods for analyte detection with magnetic sensors are provided. Aspects of the methods include producing a magnetic sensor device having a magnetically labeled analyte from a sample, such as a serum sample, bound to a surface of a magnetic sensor thereof; and obtaining a signal, e.g., a real-time signal, from the magnetic sensor to determine whether the analyte is present in the sample. Also provided are devices, systems and kits that find use in practicing the methods of the invention. The methods, devices, systems and kits of the invention find use in a variety of different applications, including detection of biomarkers, such as disease markers.
摘要翻译： 提供了使用磁传感器进行分析物检测的方法。 所述方法的方面包括制备具有磁性标记的分析物的磁性传感器装置，所述磁性传感器装置与来自其磁性传感器的表面的样品（例如血清样品）结合; 以及从磁传感器获得信号，例如实时信号，以确定分析物是否存在于样品中。 还提供了用于实践本发明的方法的装置，系统和试剂盒。 本发明的方法，装置，系统和试剂盒可用于各种不同的应用，包括检测生物标志物，例如疾病标记物。
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公开(公告)号：US07419639B2
公开(公告)日：2008-09-02
申请号：US11388223
申请日：2006-03-22
CPC分类号： B01L3/502707 , B01L9/527 , B01L2200/0689 , B01L2200/12 , B01L2300/0636 , B01L2300/0816 , B01L2300/123 , B01L2400/0415 , B01L2400/043 , B81B2201/058 , Y10T436/11 , Y10T436/25 , Y10T436/2575
摘要： The present invention provides microfluidic devices constructed from four layers. The layers include a rigid substrate layer, a patterned rigid layer having thickness t, a patterned elastomeric layer having thickness greater than t, and a rigid support layer. Microfluidic structures in the devices are defined by the alignment of openings in the patterned rigid layer and the patterned elastomeric layer. The rigid support layer, rigid substrate layer, and patterned rigid layer may be made of any rigid material, including but not limited to plastic or silicon-containing materials, such as glass, quartz, or SiO2-coated materials. Similarly, the patterned elastomeric layer may be made of any elastomeric material, including but not limited to polydimethylsiloxanes, polymethylmethacrylates, perfluoropolyethers, or combinations thereof. Microfluidic devices according to the present invention may include sensors or sensor arrays. The microfluidic devices are fabricated using the provided error-tolerant alignment, biocompatible process.
摘要翻译： 本发明提供了由四层构成的微流体装置。 这些层包括刚性基底层，具有厚度t的图案化刚性层，厚度大于t的图案化弹性体层和刚性支撑层。 器件中的微流体结构通过图案化刚性层和图案化的弹性体层中的开口的对准来限定。 刚性支撑层，刚性基底层和图案化刚性层可以由任何刚性材料制成，包括但不限于塑料或含硅材料，例如玻璃，石英或SiO 2 - 涂层材料。 类似地，图案化的弹性体层可以由任何弹性体材料制成，包括但不限于聚二甲基硅氧烷，聚甲基丙烯酸甲酯，全氟聚醚或其组合。 根据本发明的微流体装置可以包括传感器或传感器阵列。 使用提供的容错对准，生物相容性方法制造微流体装置。
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4.发明授权公开(公告)号：US09151763B2
公开(公告)日：2015-10-06
申请号：US13416928
申请日：2012-03-09
IPC分类号： G01N27/00 , G01N33/58 , C40B60/12 , B01L3/00 , B82Y15/00 , B82Y30/00 , G01N27/327 , G01R33/12 , G01N27/74 , G01N35/00 , G01R33/00 , B01L9/00 , B82Y10/00 , G01N35/02
CPC分类号： G01N33/585 , B01J2219/00317 , B01J2219/00655 , B01J2219/00662 , B01L3/5085 , B01L9/523 , B01L2300/0829 , B82Y10/00 , B82Y15/00 , B82Y30/00 , C40B60/12 , G01N27/3276 , G01N27/745 , G01N33/587 , G01N35/0098 , G01N35/026 , G01R33/0094 , G01R33/1269 , Y10S977/773 , Y10S977/92
摘要： Provided are high-throughput detection systems. The systems include a magnetic sensor device, a magnetic field source and a reservoir plate that includes a plurality of fluid reservoirs. The magnetic sensor device includes a support with two or more elongated regions each having a magnetic sensor array disposed at a distal end. Also provided are methods in which the subject high-throughput detection systems find use.
摘要翻译： 提供高通量检测系统。 这些系统包括磁传感器装置，磁场源和包括多个流体储存器的储存器板。 磁传感器装置包括具有两个或多个细长区域的支撑件，每个细长区域具有设置在远端的磁性传感器阵列。 还提供了本发明的高通量检测系统使用的方法。
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5.发明申请公开(公告)号：US20120231960A1
公开(公告)日：2012-09-13
申请号：US13416928
申请日：2012-03-09
CPC分类号： G01N33/585 , B01J2219/00317 , B01J2219/00655 , B01J2219/00662 , B01L3/5085 , B01L9/523 , B01L2300/0829 , B82Y10/00 , B82Y15/00 , B82Y30/00 , C40B60/12 , G01N27/3276 , G01N27/745 , G01N33/587 , G01N35/0098 , G01N35/026 , G01R33/0094 , G01R33/1269 , Y10S977/773 , Y10S977/92
摘要： Provided are high-throughput detection systems. The systems include a magnetic sensor device, a magnetic field source and a reservoir plate that includes a plurality of fluid reservoirs. The magnetic sensor device includes a support with two or more elongated regions each having a magnetic sensor array disposed at a distal end. Also provided are methods in which the subject high-throughput detection systems find use.
摘要翻译： 提供高通量检测系统。 这些系统包括磁传感器装置，磁场源和包括多个流体储存器的储存器板。 磁传感器装置包括具有两个或多个细长区域的支撑件，每个细长区域具有设置在远端的磁性传感器阵列。 还提供了本发明的高通量检测系统使用的方法。
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公开(公告)号：US08405385B2
公开(公告)日：2013-03-26
申请号：US12661158
申请日：2010-03-10
CPC分类号： G01R33/1269 , B82Y25/00 , G01R33/09 , G01R33/093
摘要： Double modulation of a magnetoresistive sensor entails modulating both an excitation (e.g., voltage or current) applied to the sensor and a tickling magnetic field applied to the sensor. The excitation and magnetic field are modulated at different frequencies fc and ff, respectively. As a result of the double modulation, the sensor output spectrum includes a carrier tone (CT) at frequency fc and side tones (STs) at frequencies fc±ff. A baseline relation between CT amplitude and ST amplitude is determined (e.g., by measuring CT and ST amplitude while drift occurs in the absence of a sample). During sensor operation, raw ST measurements are corrected using corresponding raw CT measurements to provide corrected ST measurements as the sensor output.
摘要翻译： 磁阻传感器的双重调制需要调制施加到传感器的激励（例如，电压或电流）以及施加到传感器的挠曲磁场。 励磁和磁场分别以不同频率fc和ff进行调制。 作为双调制的结果，传感器输出频谱包括频率为fc的载波音（CT）和频率fc±ff的侧音（ST）。 确定CT幅度和ST幅度之间的基线关系（例如，通过测量CT和ST振幅，而在不存在样本的情况下发生漂移）。 在传感器操作期间，使用相应的原始CT测量来校正原始ST测量值，以提供校正的ST测量作为传感器输出。
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公开(公告)号：US20100231203A1
公开(公告)日：2010-09-16
申请号：US12661158
申请日：2010-03-10
CPC分类号： G01R33/1269 , B82Y25/00 , G01R33/09 , G01R33/093
摘要： Double modulation of a magnetoresistive sensor entails modulating both an excitation (e.g., voltage or current) applied to the sensor and a tickling magnetic field applied to the sensor. The excitation and magnetic field are modulated at different frequencies fc and ff, respectively. As a result of the double modulation, the sensor output spectrum includes a carrier tone (CT) at frequency fc and side tones (STs) at frequencies fc±ff. A baseline relation between CT amplitude and ST amplitude is determined (e.g., by measuring CT and ST amplitude while drift occurs in the absence of a sample). During sensor operation, raw ST measurements are corrected using corresponding raw CT measurements to provide corrected ST measurements as the sensor output.
摘要翻译： 磁阻传感器的双重调制需要调制施加到传感器的激励（例如，电压或电流）以及施加到传感器的挠曲磁场。 励磁和磁场分别以不同频率fc和ff进行调制。 作为双调制的结果，传感器输出频谱包括频率为fc的载波音（CT）和频率fc±ff的侧音（ST）。 确定CT幅度和ST幅度之间的基线关系（例如，通过测量CT和ST振幅，而在不存在样本的情况下发生漂移）。 在传感器操作期间，使用相应的原始CT测量来校正原始ST测量值，以提供校正的ST测量作为传感器输出。
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公开(公告)号：US07766993B2
公开(公告)日：2010-08-03
申请号：US11830870
申请日：2007-07-31
申请人： Shouheng Sun , Heng Yu , Shan X. Wang
发明人： Shouheng Sun , Heng Yu , Shan X. Wang
IPC分类号： B22F1/02
CPC分类号： C01G1/00 , B22F1/0022 , B22F1/0096 , B22F2999/00 , B82Y25/00 , B82Y30/00 , C01B17/20 , C01B19/007 , C01P2002/72 , C01P2002/84 , C01P2004/04 , C01P2004/30 , C01P2004/64 , C01P2004/80 , C01P2006/42 , C09C3/063 , H01F1/0054 , Y10S977/835 , B22F1/0018
摘要： Dumbbell-shaped or flower-shaped nanoparticles and a process of forming the same, wherein the process comprises forming a mixture of a nanoparticle with a precursor in a first solvent, wherein the nanoparticle comprises a hydrophobic outer coating; heating the mixture; cooling the mixture to room temperature; modifying the hydrophobic outer coating into a hydrophilic outer coating; precipitating a solid product from the mixture, and dispersing the product in a second solvent. The nanoparticles comprise any of a semiconducting, magnetic, and noble metallic material, wherein the nanoparticles comprise a first portion comprising any of PbSe, PbS, CdSe, CdS, ZnS, Au, Ag, Pd, and Pt, and wherein the precursor comprises any of a cationic, neutral or particulate Au, Ag, Pd, Pt, or transition metal (Fe, Co, Ni) precursors of Fe(CO)5, Co(CO)8, Ni(CO)4 or their analogues. The first and second solvents comprise any of alkanes, arenes, ethers, nitrites, ketones, and chlorinated hydrocarbons.
摘要翻译： 哑铃形或花形纳米颗粒及其形成方法，其中所述方法包括在第一溶剂中形成纳米颗粒与前体的混合物，其中所述纳米颗粒包含疏水外涂层; 加热混合物; 将混合物冷却至室温; 将疏水性外涂层改性为亲水性外涂层; 从混合物中沉淀出固体产物，并将产物分散在第二溶剂中。 纳米颗粒包括半导体，磁性和贵金属材料中的任何一种，其中纳米颗粒包括包含PbSe，PbS，CdSe，CdS，ZnS，Au，Ag，Pd和Pt中的任何一种的第一部分，并且其中前体包含任何 的Fe（CO）5，Co（CO）8，Ni（CO）4或其类似物的阳离子，中性或微粒Au，Ag，Pd，Pt或过渡金属（Fe，Co，Ni）前体。 第一和第二溶剂包括任何烷烃，芳烃，醚，亚硝酸盐，酮和氯代烃。
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公开(公告)号：US07615382B2
公开(公告)日：2009-11-10
申请号：US11595818
申请日：2006-11-09
申请人： Shan X. Wang , Nader Pourmand , Robert L. White
发明人： Shan X. Wang , Nader Pourmand , Robert L. White
IPC分类号： G01N33/553 , B03C1/30
CPC分类号： B03C1/0335 , B03C2201/18 , B03C2201/22
摘要： The present invention provides a magnetic sifter that is small in scale, enables three-dimensional flow in a direction normal to the substrate, allows relatively higher capture rates and higher flow rates, and provides a relatively easy method of releasing captured biomolecules. The magnetic sifter includes at least one substrate. Each substrate contains a plurality of slits, each of which extends through the substrate. The sifter also includes a plurality of magnets attached to the bottom surface of the substrate. These magnets are located proximal to the openings of the slits. An electromagnetic source controls the magnitude and direction of magnetic field gradient generated by the magnets. Either one device may be used, or multiple devices may be used in series. In addition, the magnetic sifter may be used in connection with a detection chamber.
摘要翻译： 本发明提供一种规模小的磁选筛机，使得能够沿垂直于基底的方向进行三维流动，允许相对较高的捕获率和较高的流速，并且提供了一种相对容易的释放捕获的生物分子的方法。 磁选机包括至少一个衬底。 每个基板包含多个狭缝，每个狭缝延伸穿过基板。 筛子还包括附着到基底的底表面的多个磁体。 这些磁体位于缝隙的开口附近。 电磁源控制由磁体产生的磁场梯度的大小和方向。 可以使用一个设备，或者可以串联使用多个设备。 此外，磁选机可以与检测室结合使用。
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公开(公告)号：US07238486B2
公开(公告)日：2007-07-03
申请号：US11231657
申请日：2005-09-20
申请人： Nader Pourmand , Ronald W. Davis , Shan X. Wang
发明人： Nader Pourmand , Ronald W. Davis , Shan X. Wang
CPC分类号： C12Q1/68 , C12Q1/6837 , C12Q2565/519 , C12Q2537/1373 , C12Q2525/204
摘要： A method of determining the length of a polynucleotide target is provided. With this method, a target is first hybridized to an array of first probes having different, determined lengths, resulting in the formation of duplexes between the polynucleotide target and the first probes. These duplexes have a single stranded section of target if the target is longer than the first probe it is in a duplex with. Next, a second probe having a determined length is hybridized to these duplexes. If the length of the target is greater than the length of the first probe it is displaced during this hybridization step by the process of branch migration. In contrast, if the length of the target is less than or equal to the length of the first probe, it is not displaced. Thus, the length of the polynucleotide target can be determined.
摘要翻译： 提供了确定多核苷酸靶标长度的方法。 利用该方法，首先将靶与具有不同的确定长度的第一探针阵列杂交，导致在多核苷酸靶和第一探针之间形成双链体。 如果目标比第一个探针更长，那么这些双链体具有目标单链段。 接下来，具有确定长度的第二探针与这些双链体杂交。 如果靶的长度大于第一探针的长度，则在该杂交步骤期间通过分支迁移的过程被置换。 相反，如果目标的长度小于或等于第一探针的长度，则其不被移位。 因此，可以确定多核苷酸靶的长度。
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