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公开(公告)号：US11235576B2
公开(公告)日：2022-02-01
申请号：US16943129
申请日：2020-07-30
发明人： Hirokazu Sekino , Takeshi Seto , Hideki Kojima , Takahiro Matsuzaki , Yuji Saito
IPC分类号： B41J2/14
摘要： A liquid ejection device includes: a nozzle through which a liquid is ejected; a liquid transfer tube through which the liquid is transferred to the nozzle; and a vibration generation unit configured to generate vibration, in which the vibration generation unit is in contact with one of the liquid, the nozzle, and the liquid transfer tube, and when the liquid ejected from the nozzle flies as a plurality of droplets in a state where the vibration generation unit does not generate vibration, and the number of the droplets passing through a predetermined position in a unit time is defined as a droplet frequency, a frequency of the vibration generated by the vibration generation unit is equal to or less than the droplet frequency.
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公开(公告)号：US11701886B2
公开(公告)日：2023-07-18
申请号：US17643255
申请日：2021-12-08
发明人： Hirokazu Sekino , Takeshi Seto , Hideki Kojima , Takahiro Matsuzaki , Yuji Saito
IPC分类号： B41J2/14
CPC分类号： B41J2/14233
摘要： A liquid ejection device includes: a nozzle through which a liquid is ejected; a liquid transfer tube through which the liquid is transferred to the nozzle; and a vibration generation unit configured to generate vibration, in which the vibration generation unit is in contact with one of the liquid, the nozzle, and the liquid transfer tube, and when the liquid ejected from the nozzle flies as a plurality of droplets in a state where the vibration generation unit does not generate vibration, and the number of the droplets passing through a predetermined position in a unit time is defined as a droplet frequency, a frequency of the vibration generated by the vibration generation unit is equal to or less than the droplet frequency.
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公开(公告)号：US20220097375A1
公开(公告)日：2022-03-31
申请号：US17643255
申请日：2021-12-08
发明人： Hirokazu SEKINO , Takeshi Seto , Hideki Kojima , Takahiro Matsuzaki , Yuji Saito
IPC分类号： B41J2/14
摘要： A liquid ejection device includes: a nozzle through which a liquid is ejected; a liquid transfer tube through which the liquid is transferred to the nozzle; and a vibration generation unit configured to generate vibration, in which the vibration generation unit is in contact with one of the liquid, the nozzle, and the liquid transfer tube, and when the liquid ejected from the nozzle flies as a plurality of droplets in a state where the vibration generation unit does not generate vibration, and the number of the droplets passing through a predetermined position in a unit time is defined as a droplet frequency, a frequency of the vibration generated by the vibration generation unit is equal to or less than the droplet frequency.
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公开(公告)号：US20140273201A1
公开(公告)日：2014-09-18
申请号：US14204012
申请日：2014-03-11
发明人： Yuji Saito , Fumio Takagi
IPC分类号： B01L3/00
CPC分类号： B01L3/502784 , B01L3/502761 , B01L7/525 , B01L2200/0647 , B01L2200/0673 , B01L2300/0838 , B01L2400/043 , B01L2400/0478 , G01N35/0098 , G01N2021/6441 , G01N2021/6482
摘要： A cartridge for nucleic acid amplification reaction includes a tube that has a first plug formed of a oil, a second plug formed of a first washing solution which washes nucleic acid-binding solid-phase carriers having bound to a nucleic acid, a third plug formed of a oil, a fourth plug formed of an eluate which causes the nucleic acid to be eluted from the nucleic acid-binding solid-phase carriers, a fifth plug formed of a oil, a sixth plug formed of a nucleic acid amplification reaction solution which causes a nucleic acid amplification reaction, and a seventh plug formed of a oil in this order in the inside of the tube; a container for nucleic acid amplification reaction that contains oil; and a plunger that pushes liquid to the container for nucleic acid amplification reaction out of the side of the seventh plug of the tube.
摘要翻译： 用于核酸扩增反应的滤筒包括具有由油形成的第一塞子的管，由第一洗涤溶液形成的第二塞子，该第一洗涤溶液洗涤与核酸结合的核酸结合固相载体，形成第三塞子 的油，由洗脱液形成的第四塞子，其使核酸从核酸结合固相载体中洗脱出来，第五塞子由油形成，第六塞子由核酸扩增反应溶液形成，所述第六塞子由核酸扩增反应溶液形成， 在管的内部依次形成核酸扩增反应和由油组成的第七塞子; 用于含有油的核酸扩增反应的容器; 以及柱塞，其将液体推送到所述管的第七塞子侧的用于核酸扩增反应的容器。
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公开(公告)号：US20140273198A1
公开(公告)日：2014-09-18
申请号：US14204047
申请日：2014-03-11
发明人： Yuji Saito , Fumio Takagi
IPC分类号： C12M1/40
CPC分类号： B01L3/502761 , B01L3/502 , B01L3/502784 , B01L7/525 , B01L2200/0647 , B01L2200/0673 , B01L2300/0838 , B01L2400/043 , B01L2400/0478 , G01N35/0098 , G01N2035/00366 , G01N2035/0436
摘要： A cartridge for nucleic acid amplification reaction includes a tube that has a first plug, a second plug formed of a second washing solution washes nucleic acid-binding solid-phase carriers having bound to a nucleic acid, a third plug, a fourth plug formed of a reverse transcription reaction solution which undergoes phase separation when being mixed with oil and in which a reverse transcription reaction occurs, a fifth plug formed of a third oil, a sixth plug formed of an eluate which causes the nucleic acid to be eluted from the nucleic acid-binding solid-phase carriers having bound to the nucleic acid, and a seventh plug in this order in the inside of the tube; a container for nucleic acid amplification reaction that is in communication with the tube and contains oil; and a plunger that pushes liquid to the container for nucleic acid amplification reaction out of the tube.
摘要翻译： 用于核酸扩增反应的滤筒包括具有第一塞子的管，由第二洗涤溶液形成的第二塞子洗涤与核酸结合的核酸结合固相载体，第三塞子，由 与油混合并发生逆转录反应时发生相分离的逆转录反应溶液，由第三油形成的第五塞子，由洗脱液形成的第六塞子，其使核酸从核酸中洗脱出来 与核酸结合的酸结合固相载体，以及第七个塞子，依次位于管内; 用于与所述管连通且含有油的核酸扩增反应的容器; 以及将液体推出到用于核酸扩增反应的容器中的柱塞。
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公开(公告)号：US20140273100A1
公开(公告)日：2014-09-18
申请号：US14203994
申请日：2014-03-11
发明人： Yuji Saito , Fumio Takagi
IPC分类号： C12P19/34
CPC分类号： C12P19/34 , C12N15/1096
摘要： A cDNA synthesis method includes: mixing a lysis solution containing a chaotropic substance and a nucleic acid-binding solid-phase carrier in a sample containing a ribonucleic acid (RNA), thereby adsorbing the RNA on the carrier; reverse-transcribing the RNA adsorbed on the carrier while keeping the RNA adsorbed on the carrier in a reverse transcription reaction mixture, thereby synthesizing cDNA; and eluting the synthesized cDNA with an eluent.
摘要翻译： cDNA合成方法包括：在含有核糖核酸（RNA）的样品中混合含有离液物质和核酸结合固相载体的裂解液，从而将RNA吸附在载体上; 反转录载体上吸附的RNA，同时将RNA吸附在载体上的逆转录反应混合物中，从而合成cDNA; 并用洗脱液洗脱合成的cDNA。
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公开(公告)号：US09416386B2
公开(公告)日：2016-08-16
申请号：US14203994
申请日：2014-03-11
发明人： Yuji Saito , Fumio Takagi
CPC分类号： C12P19/34 , C12N15/1096
摘要： A cDNA synthesis method includes: mixing a lysis solution containing a chaotropic substance and a nucleic acid-binding solid-phase carrier in a sample containing a ribonucleic acid (RNA), thereby adsorbing the RNA on the carrier; reverse-transcribing the RNA adsorbed on the carrier while keeping the RNA adsorbed on the carrier in a reverse transcription reaction mixture, thereby synthesizing cDNA; and eluting the synthesized cDNA with an eluent.
摘要翻译： cDNA合成方法包括：在含有核糖核酸（RNA）的样品中混合含有离液物质和核酸结合固相载体的裂解液，从而将RNA吸附在载体上; 反转录载体上吸附的RNA，同时将RNA吸附在载体上的逆转录反应混合物中，从而合成cDNA; 并用洗脱液洗脱合成的cDNA。
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公开(公告)号：US09157922B2
公开(公告)日：2015-10-13
申请号：US14330548
申请日：2014-07-14
发明人： Yuji Saito , Fumio Takagi
CPC分类号： G01N35/1002 , B01L3/0241 , B01L7/52 , B01L2300/0838 , B01L2400/0457 , B01L2400/049 , G01N1/10 , G01N1/14 , G01N1/38 , G01N35/1016 , Y10T436/118339 , Y10T436/119163 , Y10T436/25 , Y10T436/2575
摘要： A dispensing method which dispenses a first liquid contained in a first vessel which stores the first liquid and a second liquid to introduce the first liquid into a second vessel by using a tube.
摘要翻译： 一种分配方法，其分配容纳在存储第一液体的第一容器中的第一液体和第二液体，以通过使用管将第一液体引入第二容器。
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公开(公告)号：US08889360B2
公开(公告)日：2014-11-18
申请号：US14060132
申请日：2013-10-22
发明人： Yuji Saito , Fumio Takagi
CPC分类号： B01L7/52 , B01L3/5082 , B01L7/525 , B01L2300/1872 , B01L2400/0409 , B01L2400/0457
摘要： A thermal cycler includes a holder to which a biotip having a longitudinal direction is attached in such a manner that one end portion of the biotip is at a higher level than the other end portion, and that the distance between one end portion of the biotip and the rotational axis is shorter than the distance between the other end portion of the biotip and the rotational axis, a heating unit heats a first end portion of the biotip, a rotating unit rotates the holder, and a controller that controls the rotation speed of the rotating unit. The controller has a first mode a rotation speed at which the magnitude of the centrifugal force acting on the reaction mixture becomes smaller than the gravity, and a second mode a rotation speed at which the magnitude of the centrifugal force acting on the reaction mixture becomes greater than the gravity.
摘要翻译： 热循环仪包括保持器，具有长度方向的生物体以这样的方式被附着，使得生物体的一个端部处于比另一个端部更高的水平，并且生物体的一个端部之间的距离和 旋转轴短于生物体的另一端部与旋转轴的距离，加热单元加热生物体的第一端部，旋转单元使保持器旋转，控制器控制旋转轴的旋转速度 旋转单元。 控制器具有第一模式，作用在反应混合物上的离心力的大小变得小于重力的旋转速度，第二模式是作用在反应混合物上的离心力的大小变大的第二模式 比重力。
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公开(公告)号：US20140273202A1
公开(公告)日：2014-09-18
申请号：US14204030
申请日：2014-03-11
发明人： Yuji Saito , Fumio Takagi
IPC分类号： B01L3/00
CPC分类号： B01L3/502784 , B01L3/502761 , B01L7/525 , B01L2200/0647 , B01L2200/0673 , B01L2300/0838 , B01L2400/043 , B01L2400/0478 , G01N35/0098 , G01N2035/00366 , G01N2035/0436
摘要： A cartridge for nucleic acid amplification reaction includes a tube that has a first plug, a second plug formed of a first washing solution which washes nucleic acid-binding solid-phase carriers having bound to a nucleic acid, a third plug, a fourth plug formed of an eluate which causes the nucleic acid to be eluted from the nucleic acid-binding solid-phase carriers having bound to the nucleic acid, and a fifth plug in this order in the inside of the tube; a container for nucleic acid amplification reaction that is in communication with the side of the fifth plug of the tube and contains oil; and a plunger that pushes liquid to the container for nucleic acid amplification reaction out of the side of the fifth plug of the tube.
摘要翻译： 用于核酸扩增反应的滤筒包括具有第一塞子的管，由洗涤与核酸结合的核酸结合固相载体的第一洗涤溶液形成的第二塞子，第三塞子，形成的第四塞子 的洗脱液，其使核酸从与核酸结合的核酸结合固相载体上洗脱，并且第五个插塞依次在管内; 用于核酸扩增反应的容器，其与所述管的所述第五塞子的一侧连通且含有油; 以及柱塞，其将液体推送到用于核酸扩增反应的容器的第五塞的侧面之外。
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