利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

9 条记录 (耗时 0.036 秒)

    Manipulation of SNF1 kinase for altered oil content in oleaginous organisms
    1.
    发明授权
    Manipulation of SNF1 kinase for altered oil content in oleaginous organisms 有权
    操纵SNF1激酶以改善含油生物体内油含量

    公开(公告)号：US08435758B2

    公开(公告)日：2013-05-07

    申请号：US12549439

    申请日：2009-08-28

    申请人： Seung-Pyo Hong ,  Quinn Qun Zhu ,  John E. Seip

    发明人： Seung-Pyo Hong ,  Quinn Qun Zhu ,  John E. Seip

    IPC分类号： C12P1/00 ,  C07H21/04 ,  C12N1/15

    CPC分类号： C12P7/6472 ,  C12P7/6427 ,  C12P7/6463

    摘要： Methods of increasing the total lipid content in a eukaryotic cell, the total content of polyunsaturated fatty acids [“PUFAs”], and/or the ratio of desaturated fatty acids to saturated fatty acids by reducing the activity of the heterotrimeric SNF1 protein kinase are disclosed. Preferably, the chromosomal genes encoding the Snf1 α-subunit, Gal83 β-subunit or Snf4 γ-subunit of the SNF1 protein kinase, the upstream regulatory genes encoding Sak1, Hxk2, Glk1 or Reg1, or the downstream genes encoding Rme1, Cbr1 or Snf3 are manipulated in a PUFA-producing strain of the oleaginous yeast Yarrowia lipolytica, resulting in increased total lipid content, as compared to the parent strain comprising the heterotrimeric SNF1 protein kinase not having reduced activity.

    摘要翻译： 公开了通过降低异源三聚SNF1蛋白激酶的活性来增加真核细胞中总脂质含量，多不饱和脂肪酸的总含量[“PUFA”]和/或去饱和脂肪酸与饱和脂肪酸的比例的方法 。 优选地，编码SNF1蛋白激酶的Snf1α-亚基，Gal83β-亚基或Snf4γ-亚基的染色体基因，编码Sak1，Hxk2，Glk1或Reg1的上游调控基因或编码Rme1，Cbr1或Snf3的下游基因 与含有不具有降低的活性的异源三聚SNF1蛋白激酶的亲本菌株相比，在含油酵母解脂耶氏酵母解脂耶氏酵母解脂耶氏酵母的PUFA生产菌株中操作，导致总脂质含量增加。

    MANIPULATION OF SNF1 KINASE FOR ALTERED OIL CONTENT IN OLEAGINOUS ORGANISMS
    2.
    发明申请
    MANIPULATION OF SNF1 KINASE FOR ALTERED OIL CONTENT IN OLEAGINOUS ORGANISMS 有权
    用于改变OLE内含物的SNF1激酶的操作

    公开(公告)号：US20100062502A1

    公开(公告)日：2010-03-11

    申请号：US12549439

    申请日：2009-08-28

    申请人： Seung-Pyo Hong ,  Quinn Qun Zhu ,  John E. Seip

    发明人： Seung-Pyo Hong ,  Quinn Qun Zhu ,  John E. Seip

    IPC分类号： C12P7/64 ,  C12N1/19 ,  C07C57/00 ,  C12N15/87

    CPC分类号： C12P7/6472 ,  C12P7/6427 ,  C12P7/6463

    摘要： Methods of increasing the total lipid content in a eukaryotic cell, the total content of polyunsaturated fatty acids [“PUFAs”], and/or the ratio of desaturated fatty acids to saturated fatty acids by reducing the activity of the heterotrimeric SNF1 protein kinase are disclosed. Preferably, the chromosomal genes encoding the Snf1 α-subunit, Gal83 β-subunit or Snf4 γ-subunit of the SNF1 protein kinase, the upstream regulatory genes encoding Sak1, Hxk2, Glk1 or Reg1, or the downstream genes encoding Rme1, Cbr1 or Snf3 are manipulated in a PUFA-producing strain of the oleaginous yeast Yarrowia lipolytica, resulting in increased total lipid content, as compared to the parent strain comprising the heterotrimeric SNF1 protein kinase not having reduced activity.

    摘要翻译： 公开了通过降低异源三聚SNF1蛋白激酶的活性来增加真核细胞中总脂质含量，多不饱和脂肪酸的总含量[“PUFA”]和/或去饱和脂肪酸与饱和脂肪酸的比例的方法 。 优选地，编码SNF1蛋白激酶的Snf1α-亚基，Gal83＆bgr-亚基或Snf4γ-亚基的染色体基因，编码Sak1，Hxk2，Glk1或Reg1的上游调节基因，或编码Rme1，Cbr1或 与含有不具有降低的活性的异源三聚SNF1蛋白激酶的亲本菌株相比，Snf3在含油酵母解脂耶氏酵母解脂耶氏酵母的PUFA产生菌株中被操纵，导致总脂质含量增加。

    Δ-9 desaturase and its use in making polyunsaturated fatty acids
    6.
    发明授权
    Δ-9 desaturase and its use in making polyunsaturated fatty acids 有权
    ＆Dgr; -9去饱和酶及其在制备多不饱和脂肪酸中的应用

    公开(公告)号：US07923223B2

    公开(公告)日：2011-04-12

    申请号：US11613420

    申请日：2006-12-20

    申请人： John E. Seip ,  Quinn Qun Zhu

    发明人： John E. Seip ,  Quinn Qun Zhu

    IPC分类号： C12P7/64 ,  C12N9/00 ,  C12N9/02 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04

    CPC分类号： C12P7/6427 ,  C12N9/0083 ,  C12P7/6472 ,  C12Y114/19001

    摘要： The present invention relates to a Δ9 desaturase, which has the ability to convert palmitic acid [16:0] or stearic acid [18:0] into palmitoleic acid [16:1] or oleic acid [18:1], respectively. Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding Δ9 desaturase along with a method of making long chain polyunsaturated fatty acids (PUFAs) using this Δ9 desaturase in oleaginous yeast is disclosed.

    摘要翻译： 本发明涉及一种具有将棕榈酸[16：0]或硬脂酸[18：0]分别转化为棕榈油酸[16：1]或油酸[18：1]的能力的“去饱和酶” 。 公开了分离的核酸片段和重组构建体，其包含编码＆Dgr。9去饱和酶的片段以及在含油酵母中使用该Dgr 9脱氢酶制备长链多不饱和脂肪酸（PUFA）的方法。

    Natural promoters for gene expression in C1 metabolizing bacteria
    9.
    发明授权
    Natural promoters for gene expression in C1 metabolizing bacteria 失效
    C1代谢细菌基因表达的天然启动子

    公开(公告)号：US07026464B2

    公开(公告)日：2006-04-11

    申请号：US10689200

    申请日：2003-10-20

    申请人： Deana J. Dicosimo ,  Hao Ni ,  Stephen K. Picataggio ,  John E. Seip ,  Rick W. Ye ,  Tao Wang

    发明人： Deana J. Dicosimo ,  Hao Ni ,  Stephen K. Picataggio ,  John E. Seip ,  Rick W. Ye ,  Tao Wang

    IPC分类号： C07H21/04

    CPC分类号： C12N15/74 ,  C07K14/195

    摘要： Genes have been identified in the Methylomonas genome that are responsive to various metabolic and growth conditions. The identified responsiveness of these genes allows for the use of their promoters in regulated gene expression in C1 metabolizing bacteria. In particular, the hps promoter, which in its native state drives the expression of 3-hexulose-6-phosphate synthase (HPS), was found to be useful for directing expression of heterolgous coding regions (e.g., crtZ) in the obligate methanotroph Methylomonas sp. 16a.

    摘要翻译： 已经在Methylomonas基因组中鉴定了对各种代谢和生长条件有反应的基因。 这些基因的确定的反应性允许在C1代谢细菌中使用其启动子调节的基因表达。 特别地，发现在其天然状态下驱动3-己糖-6-磷酸合成酶（HPS）表达的hps启动子可用于引导异源性编码区（例如，crtZ）在专性甲烷嗜甲基甲酸单体中表达 sp。 16a。