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1.发明授权Method for selecting reduced differentiation resistance human induced pluripotent stem cells 有权选择降低分化能力的人诱导多能干细胞的方法公开(公告)号：US08932857B2
公开(公告)日：2015-01-13
申请号：US13704584
申请日：2011-06-15
CPC分类号： C12N5/0081 , C12N5/0696 , C12N2501/15 , C12N2501/155
摘要： The present invention provides a method for selecting human induced pluripotent stem (iPS) cells which can be safely used for transplantation. That is, the present invention provides a method for selecting human iPS cells having reduced differentiation resistance, comprising the steps of: (1) inducing differentiation of human iPS cells; (2) detecting remaining undifferentiated cells after the step (1); and (3) selecting human iPS cells whose rate of remaining undifferentiated cells detected in step (2) is equivalent to or not more than that of control cells.
摘要翻译： 本发明提供了可以安全地用于移植的人诱导多能干细胞（iPS）细胞的选择方法。 也就是说，本发明提供了选择具有降低的分化阻力的人iPS细胞的方法，包括以下步骤：（1）诱导人iPS细胞的分化; （2）在步骤（1）之后检测剩余的未分化细胞; 和（3）选择在步骤（2）中检测到的剩余未分化细胞的速率等于或不超过对照细胞的人iPS细胞。
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公开(公告)号：US20130183757A1
公开(公告)日：2013-07-18
申请号：US13824087
申请日：2011-11-04
IPC分类号： C12N15/01
CPC分类号： C12N15/01 , C12N5/0696 , C12N9/12 , C12N9/18 , C12N15/86 , C12N2501/405 , C12N2501/60 , C12N2501/602 , C12N2501/603 , C12N2501/604 , C12N2501/605 , C12N2501/606 , C12N2501/999
摘要： Provided is a method of improving the efficiency of iPS cell establishment, comprising bringing one or more factors selected from the group consisting of proteins belonging to cyclin D family and nucleic acids that encode the same into contact with a somatic cell, in the step of nuclear reprogramming of the somatic cell. Also provided are a method of producing an iPS cell comprising the step of bringing the factor(s) and nuclear reprogramming substance(s) into contact with a somatic cell, an iPS cell comprising a nucleic acid that encodes a protein belonging to cyclin D family that can be obtained by the method of producing an iPS cell, and a method of somatic cell production by forcing the iPS cell to differentiate.
摘要翻译： 本发明提供了提高iPS细胞建立效率的方法，其包括在核苷酸步骤中使从属于细胞周期蛋白D家族的蛋白质中选出的一个或多个因子和编码其的核酸与体细胞接触 体细胞重编程。 还提供了一种生产iPS细胞的方法，包括使因子和核重编程物质与体细胞接触的步骤，iPS细胞包含编码属于细胞周期蛋白D家族的蛋白质的核酸 可以通过生产iPS细胞的方法获得，以及通过强迫iPS细胞分化的体细胞产生的方法。
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3.发明授权Somatic cell reprogramming by retroviral vectors encoding Oct3/4. Klf4, c-Myc and Sox2 有权通过编码Oct3 / 4的逆转录病毒载体进行体细胞重编程。 Klf4，c-Myc和Sox2公开(公告)号：US08129187B2
公开(公告)日：2012-03-06
申请号：US12656907
申请日：2010-02-18
CPC分类号： C07K14/4702 , C12N5/0696 , C12N2501/602 , C12N2501/603 , C12N2501/604 , C12N2501/606 , C12N2510/00 , C12N2740/15043
摘要： The present invention relates to a nuclear reprogramming factor having an action of reprogramming a differentiated somatic cell to derive an induced pluripotent stem (iPS) cell. The present invention also relates to the aforementioned iPS cells, methods of generating and maintaining iPS cells, and methods of using iPS cells, including screening and testing methods as well as methods of stem cell therapy. The present invention also relates to somatic cells derived by inducing differentiation of the aforementioned iPS cells.
摘要翻译： 本发明涉及具有重新编程分化的体细胞以产生诱导多能干细胞（iPS）细胞的作用的核重编程因子。 本发明还涉及上述iPS细胞，产生和维持iPS细胞的方法，以及使用iPS细胞的方法，包括筛选和测试方法以及干细胞治疗方法。 本发明还涉及通过诱导上述iPS细胞的分化衍生的体细胞。
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公开(公告)号：US20110223669A1
公开(公告)日：2011-09-15
申请号：US12672042
申请日：2009-06-26
CPC分类号： C12N5/0696 , C12N15/85 , C12N2501/60 , C12N2501/602 , C12N2501/603 , C12N2501/604 , C12N2501/606 , C12N2506/11 , C12N2510/00
摘要： The present invention provides a method of improving the efficiency of establishment of induced pluripotent stem (iPS) cells, comprising inhibiting the p53 function in the step of somatic cell nuclear reprogramming. The inhibition of p53 function is achieved by bringing a substance selected from the group consisting of (1) chemical inhibitors of p53, (2) dominant negative mutants of p53 and nucleic acids that encode the same, (3) siRNAs and shRNAs against p53 and DNAs that encode the same, and (4) p53 pathway inhibitors, into contact with a somatic cell, and the like. The present invention also provides an agent for improving the efficiency of establishment of iPS cells, the agent comprising an inhibitor of p53 function, particularly (1) chemical inhibitors of p53, (2) dominant negative mutants of p53 and nucleic acids that encode the same, (3) siRNAs and shRNAs against p53 and DNAs that encode the same, and (4) p53 pathway inhibitors. The present invention further provides a method of producing an iPS cell, comprising bringing a nuclear reprogramming substance and an inhibitor of p53 function into contact with a somatic cell.
摘要翻译： 本发明提供了提高诱导多能干细胞（iPS）细胞的建立效率的方法，包括抑制体细胞核重编程步骤中的p53功能。 通过使选自（1）p53的化学抑制剂，（2）p53的显性失活突变体和编码其的核酸，（3）siRNA和shRNA的物质与p53和 编码相同的DNA，（4）p53途径抑制剂，与体细胞接触等。 本发明还提供了用于提高iPS细胞的建立效率的试剂，该试剂包含p53功能的抑制剂，特别是（1）p53的化学抑制剂，（2）p53的显性失活突变体和编码其的核酸 ，（3）针对p53的siRNA和shRNA，编码相同的DNA，以及（4）p53途径抑制剂。 本发明还提供了一种制备iPS细胞的方法，包括使核重编程物质和p53功能抑制剂与体细胞接触。
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公开(公告)号：US20090047263A1
公开(公告)日：2009-02-19
申请号：US12213035
申请日：2008-06-13
IPC分类号： A61K35/12 , C12N5/18 , G01N33/567 , A61P43/00 , C12N5/22
CPC分类号： C07K14/4702 , C12N5/0696 , C12N2501/60 , C12N2501/602 , C12N2501/603 , C12N2501/604 , C12N2510/00 , C12N2740/15043
摘要： The present invention relates to a nuclear reprogramming factor having an action of reprogramming a differentiated somatic cell to derive an induced pluripotent stem (iPS) cell. The present invention also relates to the aforementioned iPS cells, methods of generating and maintaining iPS cells, and methods of using iPS cells, including screening and testing methods as well as methods of stem cell therapy. The present invention also relates to somatic cells derived by inducing differentiation of the aforementioned iPS cells.
摘要翻译： 本发明涉及具有重新编程分化的体细胞以产生诱导多能干细胞（iPS）细胞的作用的核重编程因子。 本发明还涉及上述iPS细胞，产生和维持iPS细胞的方法，以及使用iPS细胞的方法，包括筛选和测试方法以及干细胞治疗方法。 本发明还涉及通过诱导上述iPS细胞的分化衍生的体细胞。
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公开(公告)号：US09677141B2
公开(公告)日：2017-06-13
申请号：US14235391
申请日：2012-07-25
CPC分类号： C12Q1/6888 , C12Q1/6881 , C12Q2600/158 , G01N33/5023 , G01N33/5073
摘要： The present invention provides a method for screening for iPS cells exhibiting differentiation resistance using a marker identified as lincRNA or mRNA that is specifically expressed in an iPS cell line exhibiting differentiation resistance, and such markers.
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公开(公告)号：US20090227032A1
公开(公告)日：2009-09-10
申请号：US12289873
申请日：2008-11-06
CPC分类号： C07K14/4702 , C12N5/0696 , C12N2501/60 , C12N2501/602 , C12N2501/603 , C12N2501/604 , C12N2501/606 , C12N2510/00 , C12N2740/15043
摘要： The present invention relates to a nuclear reprogramming factor having an action of reprogramming a differentiated somatic cell to derive an induced pluripotent stem (iPS) cell. The present invention also relates to the aforementioned iPS cells, methods of generating and maintaining iPS cells, and methods of using iPS cells, including screening and testing methods as well as methods of stem cell therapy. The present invention also relates to somatic cells derived by inducing differentiation of the aforementioned iPS cells.
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8.发明申请METHOD FOR PRODUCING INDUCED PLURIPOTENT STEM CELLS AND METHOD FOR CULTURING THE SAME 有权用于产生诱导性的细胞干细胞的方法和用于培养它们的方法公开(公告)号：US20120070896A1
公开(公告)日：2012-03-22
申请号：US13322445
申请日：2010-05-28
IPC分类号： C12N5/071
CPC分类号： C12N5/0696 , C12N2501/602 , C12N2501/603 , C12N2501/604 , C12N2501/606 , C12N2502/00 , C12N2502/13 , C12N2502/1323 , C12N2510/00
摘要： [Object] To provide a technology for production of safer iPS cells and to provide a more efficient technology for culturing iPS cells.[Solving Means] Induced pluripotent stem cells are produced from human somatic cells by co-culturing human somatic cells having a reprogrammed nucleus with human cells as feeder cells. Induced pluripotent stem cells are produced from somatic cells by co-culturing somatic cells having a reprogrammed nucleus with autologous cells as feeder cells. Induced pluripotent stem cells are cultured with culture supernatant of somatic cells.
摘要翻译： [目的]提供一种用于生产更安全的iPS细胞的技术，并为培养iPS细胞提供更有效的技术。 [解决方案]诱导的多能干细胞通过将具有重编程核的人体细胞与人细胞作为饲养细胞共培养从人体细胞产生。 通过将具有重编程核的体细胞与自体细胞共培养为饲养细胞，从体细胞产生诱导的多能干细胞。 诱导的多能干细胞与体细胞的培养上清液一起培养。
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公开(公告)号：US08048999B2
公开(公告)日：2011-11-01
申请号：US12086479
申请日：2006-12-06
IPC分类号： C07H21/04
CPC分类号： C07K14/4702 , C12N5/0696 , C12N2501/60 , C12N2501/602 , C12N2501/603 , C12N2501/604 , C12N2501/605 , C12N2501/606 , C12N2510/00 , C12N2799/027
摘要： There is provided a nuclear reprogramming factor for a somatic cell, which comprises a gene product of each of the following three kinds of genes: an Oct family gene, a Klf family gene, and a Myc family gene, as a means for inducing reprogramming of a differentiated cell to conveniently and highly reproducibly establish an induced pluripotent stem cell having pluripotency and growth ability similar to those of ES cells without using embryo or ES cell.
摘要翻译： 提供了体细胞的核重编程因子，其包括以下三种基因中的每一种的基因产物：Oct家族基因，Klf家族基因和Myc家族基因，作为诱导重编程的手段 分化细胞方便地和高度可重复地建立具有多能性和生长能力的诱导多能干细胞，其具有与不使用胚胎或ES细胞的ES细胞相似的多能性和生长能力。
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公开(公告)号：US20110053267A1
公开(公告)日：2011-03-03
申请号：US12935380
申请日：2009-04-01
申请人： Hiromitsu Nakauchi , Koji Eto , Hidekazu Nishiki-I , Naoya Takayama , Shinya Yamanaka , Kazutoshi Takahashi
发明人： Hiromitsu Nakauchi , Koji Eto , Hidekazu Nishiki-I , Naoya Takayama , Shinya Yamanaka , Kazutoshi Takahashi
IPC分类号： C12N5/078 , C12N5/0789
CPC分类号： C12N5/0644 , A61K35/12 , C12N2501/125 , C12N2501/145 , C12N2501/165 , C12N2501/91 , C12N2502/1394 , C12N2506/45
摘要： It is an object of the present invention to provide a method for efficiently preparing blood cells, such as mature megakaryocytes and platelets, from iPS cells in an in vitro culture system.The present invention provides a sac-like structure enclosing hematopoietic progenitor cells, which is obtained by inoculating iPS cells onto feeder cells and then culturing the iPS cells under conditions suitable for inducing the differentiation of hematopoietic progenitor cells. Moreover, the present invention also provides a method for producing various types of blood cells, which comprises culturing hematopoietic progenitor cells enclosed in the sac-like structure under conditions suitable for inducing the differentiation of blood cells. Furthermore, the present invention also provides a method for producing various types of blood cells, particularly megakaryocytes and platelets, without involving the sac-like structure.
摘要翻译： 本发明的目的是在体外培养系统中提供从iPS细胞中有效地制备血细胞如成熟巨核细胞和血小板的方法。 本发明提供封闭造血祖细胞的囊状结构，其通过将iPS细胞接种在饲养细胞上，然后在适于诱导造血祖细胞分化的条件下培养iPS细胞而获得。 此外，本发明还提供了生产各种血细胞的方法，其包括在适于诱导血细胞分化的条件下培养包封在囊状结构中的造血祖细胞。 此外，本发明还提供了不涉及囊状结构来生产各种类型的血细胞，特别是巨核细胞和血小板的方法。
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