Computer algorithm for automatic allele determination from fluorometer genotyping device
    1.
    发明申请
    Computer algorithm for automatic allele determination from fluorometer genotyping device 审中-公开
    用于荧光计基因分型装置自动等位基因测定的计算机算法

    公开(公告)号:US20080082273A1

    公开(公告)日:2008-04-03

    申请号:US11899199

    申请日:2007-09-05

    IPC分类号: G01N33/48

    摘要: The present invention provides methods and systems for an automated method of identifying allele values from data files derived from processed fluorophore emissions detected during the observation of fluorophore labeled nucleotide probes used in analyzing polymorphic DNA are provided. These methods are used in the rapid and efficient distinguishing of targeted polymorphic DNA sites without control samples.

    摘要翻译: 本发明提供了用于从用于分析多态性DNA的荧光团标记的核苷酸探针观察期间检测到的经处理的荧光团发射的数据文件中鉴定等位基因值的自动化方法的方法和系统。 这些方法用于快速高效地区分不含对照样品的靶向多态性DNA位点。

    Probe composition and method
    7.
    发明申请
    Probe composition and method 失效
    探头组成和方法

    公开(公告)号:US20050112609A1

    公开(公告)日:2005-05-26

    申请号:US10825624

    申请日:2004-04-14

    IPC分类号: C12Q1/68

    摘要: Method and composition for detecting one or more selected polynucleotide regions in a target polynucleotide. In the method, a mixture of sequence-specific probes are reacted with the target polynucleotide under hybridization conditions, and the hybridized probes are treated to selectively modify those probes which are bound to the target polynucleotide in a base-specific manner. The resulting labeled probes include a polymer chain which imparts to each different-sequence probe, a distinctive ratio of charge/translational frictional drag, and a detectable label. The labeled probes are fractionated by electrophoresis in a non-sieving matrix, and the presence of one or more selected sequences in the target polynucleotide are detected according to the observed electrophoretic migration rates of the labeled probes in a non-sieving medium.

    摘要翻译: 用于检测靶多核苷酸中的一个或多个选择的多核苷酸区域的方法和组合物。 在该方法中,将序列特异性探针的混合物与靶多核苷酸在杂交条件下反应,并对杂交的探针进行处理以选择性地修饰以碱基特异性方式结合靶多核苷酸的那些探针。 所得到的标记探针包括赋予每个不同序列探针的聚合物链,电荷/平移摩擦阻力的独特比例和可检测标记。 标记的探针通过在非筛选基质中的电泳分级分离,并且根据在非筛选培养基中标记的探针的观察到的电泳迁移速率来检测靶多核苷酸中一个或多个选定序列的存在。