公开(公告)号：US20170248512A1

公开(公告)日：2017-08-31

申请号：US15320286

申请日：2015-06-26

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Dino Di Carlo ,  Derek Go ,  Mahdokht Masaeli ,  Elodie Sollier

IPC: G01N15/14 ,  G01N33/493 ,  G06T7/00 ,  G01N33/49

Abstract: A system and method for the label-free analysis of cells includes a purification device configured to receive a heterogeneous population of cells, the purification device temporarily trapping therein a subpopulation of cells from the heterogeneous population of cells and a cell analysis device positioned downstream of the purification device and configured to measure one or more cellular parameters including cell count, measured cell size, and/or cell morphology. In an alternative embodiment, the subpopulation of cells is analyzed while they are trapped within the purification device.

公开(公告)号：US09333510B2

公开(公告)日：2016-05-10

申请号：US14346290

申请日：2012-09-27

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Dino Di Carlo ,  Mahdokht Masaeli ,  Elodie Sollier

IPC: B03B5/48 ,  G01N1/40 ,  G01N35/08 ,  G01N15/02 ,  B01L3/00 ,  G01N15/00

CPC classification number: B03B5/48 ,  B01L3/502746 ,  B01L3/502761 ,  B01L2200/0636 ,  B01L2200/0652 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/088 ,  B01L2400/0487 ,  G01N1/4077 ,  G01N15/02 ,  G01N35/08 ,  G01N2015/0065 ,  G01N2015/0288 ,  G01N2015/0294

Abstract: A particle sorting system includes an inlet and an inertial focusing microchannel disposed in a substrate and having a downstream expanding region at a distal end, wherein the inlet is connected to an upstream end of the microchannel. A source of different shaped particles is connected to the inlet, wherein the source of different shaped particles are configured for continuous introduction into the inlet. A plurality of outlets is connected to the microchannel at the downstream expanding region. Fluidic resistors are located in the respective outlets. Different resistances may be used in the outlets to capture enriched fractions of particles having particular particle shape(s).

Abstract translation: 颗粒分选系统包括设置在基板中的入口和惯性聚焦微通道，并且在远端具有下游扩张区域，其中入口连接到微通道的上游端。 不同成形颗粒的源连接到入口，其中不同成形颗粒的源被构造成用于连续引入入口。 多个出口在下游扩展区域连接到微通道。 流体电阻位于相应的出口。 可以在出口中使用不同的电阻来捕获具有特定颗粒形状的颗粒的富集部分。

公开(公告)号：US20140224710A1

公开(公告)日：2014-08-14

申请号：US14346290

申请日：2012-09-27

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Dino Di Carlo ,  Mahdokht Masaeli ,  Elodie Sollier

IPC: B03B5/48 ,  G01N35/08

CPC classification number: B03B5/48 ,  B01L3/502746 ,  B01L3/502761 ,  B01L2200/0636 ,  B01L2200/0652 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/088 ,  B01L2400/0487 ,  G01N1/4077 ,  G01N15/02 ,  G01N35/08 ,  G01N2015/0065 ,  G01N2015/0288 ,  G01N2015/0294

Abstract: A particle sorting system includes an inlet and an inertial focusing microchannel disposed in a substrate and having a downstream expanding region at a distal end, wherein the inlet is connected to an upstream end of the microchannel. A source of different shaped particles is connected to the inlet, wherein the source of different shaped particles are configured for continuous introduction into the inlet. A plurality of outlets is connected to the microchannel at the downstream expanding region. Fluidic resistors are located in the respective outlets. Different resistances may be used in the outlets to capture enriched fractions of particles having particular particle shape(s).

Abstract translation: 颗粒分选系统包括设置在基板中的入口和惯性聚焦微通道，并且在远端具有下游扩张区域，其中入口连接到微通道的上游端。 不同成形颗粒的源连接到入口，其中不同成形颗粒的源被构造成用于连续引入入口。 多个出口在下游扩展区域连接到微通道。 流体电阻位于相应的出口。 可以在出口中使用不同的电阻来捕获具有特定颗粒形状的颗粒的富集部分。

公开(公告)号：US20140230909A1

公开(公告)日：2014-08-21

申请号：US14347047

申请日：2012-09-27

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Dino Di Carlo ,  Hamed Amini ,  Elodie Sollier

IPC: F15D1/14

CPC classification number: F15D1/14 ,  B01J19/0093 ,  B01J2219/00855 ,  B01J2219/00889 ,  B01J2219/00995 ,  B01L3/502707 ,  B01L3/502776 ,  B01L7/00 ,  B01L2200/0636 ,  B01L2200/0652 ,  B01L2300/0816 ,  B01L2400/086 ,  Y10T137/0324 ,  Y10T137/86928

Abstract: A microfluidic platform is disclosed that uses obstacles placed at particular location(s) within the channel cross-section to turn and stretch fluid. The asymmetric flow behavior upstream and downstream of the obstacle(s) due to fluid inertia manifests itself as a total deformation of the topology of streamlines that effectively creates a tunable net secondary flow. The system and methods passively creates strong secondary flows at moderate to high flow rates in microchannels. These flows can be accurately controlled by the numbers and particular geometric placement of the obstacle(s) within the channel.

Abstract translation: 公开了一种微流体平台，其使用放置在通道横截面内的特定位置处的障碍物来旋转和拉伸流体。 由于流体惯性，障碍物的上游和下游的不对称流动行为本身就表现为有效地产生可调谐净次级流的流线拓扑的总体变形。 该系统和方法被动地在微通道中产生中等到高流量的强二次流。 这些流量可以通过通道内的障碍物的数量和特定的几何位置来精确地控制。