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公开(公告)号：US5677141A
公开(公告)日：1997-10-14
申请号：US314309
申请日：1994-09-30
申请人： Takao Isogai , Masao Fukagawa , Morita Iwami , Ichiro Aramori , Hitoshi Kojo
发明人： Takao Isogai , Masao Fukagawa , Morita Iwami , Ichiro Aramori , Hitoshi Kojo
IPC分类号： C12N15/09 , C12N1/15 , C12N1/21 , C12N9/06 , C12N9/58 , C12N9/80 , C12N15/55 , C12N15/57 , C12N15/80 , C12P17/00 , C12P17/18 , C12P35/02 , C12R1/01 , C12R1/645 , C12P15/00
CPC分类号： C12N9/0024 , C12N15/80 , C12N9/58 , C12N9/80 , C12P35/02
摘要： The present invention provides a process for producing 7-aminocephem compounds or salts thereof. 7-Aminocephem compounds are produced via microorganisms transformed with a vector containing a gene capable of converting a cephalosporin compound of the formula (II): ##STR1## to a 7-aminocephem compound of the formula (I): ##STR2##
摘要翻译： 本发明提供了一种生产7-氨基头孢烯化合物或其盐的方法。 7-氨基头孢烯化合物通过用含有能够将式（II）的头孢菌素化合物转化为（IMA）的基因的载体转化成通式（I）的7-氨基头孢烯化合物转化的微生物：
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公开(公告)号：US5192678A
公开(公告)日：1993-03-09
申请号：US747901
申请日：1991-08-20
申请人： Morita Iwami , Ichiro Aramori , Masao Fukagawa , Takao Isogai , Hitoshi Kojo
发明人： Morita Iwami , Ichiro Aramori , Masao Fukagawa , Takao Isogai , Hitoshi Kojo
摘要： A cephalosporin C acylase from Pseudomonas diminuta N-176 is disclosed as well as the recombinant production of this enzyme in E. coli. The enzyme is characterized by the ability to catalyze the conversion of cephalosporin C, gultaryl 7-ACA, apidyl 7-ACA, succinyl 7-ACA, N-acetylcephalosporin C, N-benzoylcephalosporin C, and cephalothin into 7-aminocephalosporanic acid and is composed of an .alpha.-subunit with a molecular weight of 26,000 daltons and a .beta.-subunit with a molecular weight of 58,000 daltons.
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公开(公告)号：US5320948A
公开(公告)日：1994-06-14
申请号：US935312
申请日：1992-08-26
申请人： Morita Iwami , Ichiro Aramori , Masao Fukagawa , Takao Isogai , Hitoshi Kojo
发明人： Morita Iwami , Ichiro Aramori , Masao Fukagawa , Takao Isogai , Hitoshi Kojo
IPC分类号： C12N1/21 , C12N9/80 , C12N15/09 , C12N15/56 , C12P35/04 , C12P35/06 , C12R1/19 , C12R1/38 , C12P35/00 , C12P21/02
摘要： A cephalosporin C acylase from Pseudomonas diminuta N-176 is characterized by its ability to catalyze the conversion of cephalosporin C, glutaryl 7-ACA, adipyl 7-ACA, succinyl 7-ACA, N-acetylcephalosporin, N-benzoylcephalosporin C and cephalothin into 7-aminocephalosporanic acid. The enzyme contains an .alpha.-subunit having a molecular weight of 26 kDA and a .beta.-subunit having a molecular weight of 58 kDa. A method for the recombinant production of the present cephalosporin C acylase in Escherichia coli is provided.
摘要翻译： 来自假单胞菌N-176的头孢菌素C酰基转移酶的特征在于其能够催化头孢菌素C，戊二酰7-ACA，己二酰基7-ACA，琥珀酰基7-ACA，N-乙酰头孢菌素，N-苯甲酰头孢菌素C和头孢噻吩转化为7 - 氨基头孢烷酸。 该酶含有分子量为26kDA的α亚基和分子量为58kDa的β亚基。 提供了在大肠杆菌中重组生产本发明头孢菌素C酰基转移酶的方法。
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公开(公告)号：US5310659A
公开(公告)日：1994-05-10
申请号：US779049
申请日：1991-10-18
申请人： Ichiro Aramori , Masao Fukagawa , Hiroki Ono , Yosuke Ishitani , Mana Tsumura , Morita Iwami , Hitoshi Kojo
发明人： Ichiro Aramori , Masao Fukagawa , Hiroki Ono , Yosuke Ishitani , Mana Tsumura , Morita Iwami , Hitoshi Kojo
摘要： A GL-7ACA acylase having the following characteristics:(a) has ability to catalyze the enzymatic conversion of glutaryl 7-ACA, adipyl 7-ACA, and succinyl 7-ACA, into 7-aminocephalosporanic acid,(b) has a molecular weight of 70,000 dalton (SDS-PAGE) and(c) has N-terminal amino acid sequence (SEQ ID NO: 1) thereof: Gln-Ser-Glu-Gln-Glu-Lys-Ala-Glu-Glu-.A process for producing GL-7ACA acylase is also provided.
摘要翻译： 具有以下特征的GL-7ACA酰基转移酶：（a）具有催化戊二酰7-ACA，己二酰基7-ACA和琥珀酰基7-ACA酶促转化成7-氨基头孢烷酸的能力，（b）具有分子量 的70,000道尔顿（SDS-PAGE）和（c）具有N-末端氨基酸序列（SEQ ID NO：1）：Gln-Ser-Glu-Gln-Glu-Lys-Ala-Glu-Glu-。 还提供了生产GL-7ACA酰基转移酶的方法。
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公开(公告)号：US5312750A
公开(公告)日：1994-05-17
申请号：US80240
申请日：1993-06-22
申请人： Ichiro Aramori , Masao Fukagawa , Hiroki Ono , Yosuke Ishitani , Mana Tsumura , Morita Iwami , Hitoshi Kojo
发明人： Ichiro Aramori , Masao Fukagawa , Hiroki Ono , Yosuke Ishitani , Mana Tsumura , Morita Iwami , Hitoshi Kojo
IPC分类号： C12N1/21 , C12N9/80 , C12N15/09 , C12N15/55 , C12P35/02 , C12R1/07 , C12R1/19 , C12D21/06 , C12N15/00
摘要： A GL-7ACA acylase having the following characteristics:(a) has ability to catalyze the enzymatic conversion of glutaryl 7-ACA, adipyl 7-ACA, and succinyl 7-ACA, into 7-aminocephalosporanic acid,(b) has a molecular weight of 70,000 dalton (SDS-PAGE) and(c) has N-terminal amino acid sequence (sea in No: 1) thereof: Gln-Ser-Glu-Gln-Glu-Lys-Ala-Glu-Glu-.A process for producing GL-7ACA acylase is also provided.
摘要翻译： 具有以下特征的GL-7ACA酰基转移酶：（a）具有催化戊二酰7-ACA，己二酰基7-ACA和琥珀酰基7-ACA酶促转化成7-氨基头孢烷酸的能力，（b）具有分子量 的70,000道尔顿（SDS-PAGE）和（c）具有N-末端氨基酸序列（海洋号：1）：Gln-Ser-Glu-Gln-Glu-Lys-Ala-Glu-Glu-。 还提供了生产GL-7ACA酰基转移酶的方法。
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6.发明申请Novel method of selecting immunosuppressant having little thrombocytopenic effect 审中-公开选择具有很小血小板减少作用的免疫抑制剂的新方法公开(公告)号：US20060135401A1
公开(公告)日：2006-06-22
申请号：US10519678
申请日：2003-07-07
申请人： Takao Fujimura , Hiroaki Mori , Katsuhiko Yoshizawa , Yoko Takata , Ichiro Aramori , Hideaki Matsuoka , Akira Unami , Takahisa Noto
发明人： Takao Fujimura , Hiroaki Mori , Katsuhiko Yoshizawa , Yoko Takata , Ichiro Aramori , Hideaki Matsuoka , Akira Unami , Takahisa Noto
IPC分类号： A61K38/17 , G01N33/567
CPC分类号： C12Q1/6897 , C12Q1/6883 , G01N33/5047 , G01N33/6869 , G01N33/6872 , G01N2333/55
摘要： The invention relates to a novel method for selecting an immunosuppressive agent with a less thrombocytopenia effect. According to the invention, a method for selecting an immunosuppressive agent which has a potent immunosuppressive activity but a lower thrombocytopenia effect, said method comprising measuring an IL-2 transcription inhibitory activity in a test cell in to which an IL-2 reporter gene has been introduced in the coexistence of an analyte, while measuring a GATA-1 transcription inhibitory activity in the test cell into which a GATA-1 reporter gene has been introduced in the coexistence of an analyte, and comparing both the transcription inhibitory activities, is provided.
摘要翻译： 本发明涉及一种选择血小板减少作用较小的免疫抑制剂的新方法。 根据本发明，一种选择具有有效的免疫抑制活性但较低的血小板减少症的免疫抑制剂的方法，所述方法包括测量IL-2转录抑制活性，其中IL-2报告基因已被 在分析物共存的同时测量在分析物共存中引入了GATA-1报道基因的测试细胞中的GATA-1转录抑制活性，并比较两种转录抑制活性。
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公开(公告)号：US20080076138A1
公开(公告)日：2008-03-27
申请号：US11664422
申请日：2006-02-24
CPC分类号： C12N9/99 , C12Q1/48 , G01N33/502
摘要： It is intended to provide a method for evaluating a pharmacological effect of a histone deacetylase (HDAC) inhibitor, comprising the steps of: (a) preparing a cell expressing CPSF5 protein; (b) contacting the cell with an HDAC inhibitor; (c) measuring the acetylation level of CPSF5 protein in the cell; and (d) comparing the acetylation level measured in the step (c) with the measured acetylation level of CPSF5 protein in a control cell that has not been contacted with the HDAC inhibitor.
摘要翻译： 本发明旨在提供一种评价组蛋白脱乙酰酶（HDAC）抑制剂的药理作用的方法，包括以下步骤：（a）制备表达CPSF5蛋白的细胞; （b）使细胞与HDAC抑制剂接触; （c）测量细胞中CPSF5蛋白的乙酰化水平; 和（d）将步骤（c）中测量的乙酰化水平与未与HDAC抑制剂接触的对照细胞中测量的CPSF5蛋白的乙酰化水平进行比较。
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公开(公告)号：US20070123452A1
公开(公告)日：2007-05-31
申请号：US10540546
申请日：2003-12-26
CPC分类号： G01N33/573 , C12Q1/34 , G01N2500/02
摘要： The present invention provides a method of selecting an IL-2 production inhibitor and/or an immunocyte proliferation inhibitor having low GATA-1 production inhibitory activity, which comprises measuring the HDAC4 and/or HDAC8 inhibitory activity of a test substance, and a method of selecting an immunosuppressant having low thrombocytopenic activity, which comprises measuring the HDAC4 and/or HDAC8 inhibitory activity of a test HDAC inhibitor.
摘要翻译： 本发明提供了选择具有低GATA-1产生抑制活性的IL-2产生抑制剂和/或免疫细胞增殖抑制剂的方法，其包括测定被检物质的HDAC4和/或HDAC8抑制活性， 选择具有低血小板减少活性的免疫抑制剂，其包括测量HDAC抑制剂的HDAC4和/或HDAC8抑制活性。
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