公开(公告)号：US5757022A

公开(公告)日：1998-05-26

申请号：US733182

申请日：1996-10-17

申请人： Takashi Kobayashi ,  Nobuhiko Ogura

发明人： Takashi Kobayashi ,  Nobuhiko Ogura

IPC分类号： G06T5/40 ,  H04N1/407 ,  H04N1/40 ,  G01N23/20

CPC分类号： H04N1/4074 ,  G06T5/009 ,  G06T5/10 ,  G06T5/40 ,  G06T2207/10064

摘要： An image processing apparatus includes a histogram producing section for producing a histogram of density signal levels of pixels constituting the image data, a reference density signal level detecting section for detecting from the histogram a density signal level processed by the maximum number of the pixels in a range excluding the maximum value and the minimum value of the density signal levels, a density signal level conversion range determining section for successively comparing the number of the pixels of each density signal level with a threshold value from the density signal level processed by the maximum number of the pixels to lower density signal levels, thereby determining a density signal level lower limit value of density signal levels to be converted and successively comparing the number of the pixels of each density signal level with a threshold value from the maximum value of the density signal level to lower density signal levels, thereby determining a density signal level upper limit value of density signal levels to be converted, a density signal level conversion value calculating section for calculating a conversion value so that density signal levels between the density signal level lower limit value and the density signal level upper limit value can be converted to density signal levels ranging from white to black and a density signal level converting section for converting the density signal levels of the pixels in accordance with the conversion value. According to the thus constituted image processing apparatus, it is possible to reproduce an electron microscope image having a desired gradation.

摘要翻译： 图像处理装置包括直方图产生部分，用于产生构成图像数据的像素的浓度信号电平的直方图;参考密度信号电平检测部分，用于从直方图检测由最大数量的像素处理的浓度信号电平 密度信号电平转换范围确定部分，用于将密度信号电平的像素数与从最大数目处理的浓度信号电平的阈值连续地比较， 从而确定要转换的浓度信号电平的浓度信号电平下限值，并将每个浓度信号电平的像素数与阈值的密度信号从密度信号的最大值相比较 电平到较低密度信号电平，从而确定密度信号 密度信号电平转换值计算部分，用于计算转换值，使密度信号电平下限值和密度信号电平上限值之间的浓度信号电平可转换为 密度信号电平范围从白色到黑色，密度信号电平转换部分根据转换值转换像素的浓度信号电平。 根据这样构成的图像处理装置，可以再现具有期望灰度的电子显微镜图像。

公开(公告)号：US07715012B2

公开(公告)日：2010-05-11

申请号：US12394298

申请日：2009-02-27

申请人： Nobuhiko Ogura ,  Hitoshi Shimizu ,  Koji Kuruma ,  Hisashi Ohtsuka ,  Tatsuo Fujikura ,  Shu Sato

发明人： Nobuhiko Ogura ,  Hitoshi Shimizu ,  Koji Kuruma ,  Hisashi Ohtsuka ,  Tatsuo Fujikura ,  Shu Sato

IPC分类号： G01N21/55

CPC分类号： G01N21/05 ,  G01N21/553 ,  G01N2021/0346 ,  G01N2021/058

摘要： A sensor unit of a surface plasmon resonance (SPR) assay system includes a transparent dielectric medium. A thin film has a first surface and a sensing surface. The first surface is connected with the dielectric medium to constitute an interface. The sensing surface is back to the first surface, for detecting (bio) chemical reaction. A flow cell block has a flow channel for flowing of the sample to the sensing surface. Attenuated total reflection of illuminating light is checked at the interface, to analyze interaction between ligand and analyte as samples. The flow channel includes a first inner surface, disposed opposite to the sensing surface to extend along, for passing the sample to flow between. The first inner surface has a height, defined with reference to the sensing surface, and in a range of 200-500 microns.

摘要翻译： 表面等离子体共振（SPR）测定系统的传感器单元包括透明电介质。 薄膜具有第一表面和感测表面。 第一表面与电介质连接以构成界面。 感测表面回到第一个表面，用于检测（生物）化学反应。 流动池块具有用于将样品流动到感测表面的流动通道。 在界面处检查照明光的衰减全反射，分析配体和分析物之间的相互作用作为样品。 流动通道包括与感测表面相对设置的第一内表面，以沿着该方式延伸以使样品在两者之间流动。 第一内表面具有相对于感测表面限定的高度，并且在200-500微米的范围内。

公开(公告)号：US20090195784A1

公开(公告)日：2009-08-06

申请号：US12394298

申请日：2009-02-27

申请人： Nobuhiko Ogura ,  Hitoshi Shimizu ,  Koji Kuruma ,  Hisashi Ohtsuka ,  Tatsuo Fujikura ,  Shu Sato

发明人： Nobuhiko Ogura ,  Hitoshi Shimizu ,  Koji Kuruma ,  Hisashi Ohtsuka ,  Tatsuo Fujikura ,  Shu Sato

IPC分类号： G01N21/55 ,  G01N21/05

CPC分类号： G01N21/05 ,  G01N21/553 ,  G01N2021/0346 ,  G01N2021/058

摘要： A sensor unit of a surface plasmon resonance (SPR) assay system includes a transparent dielectric medium. A thin film has a first surface and a sensing surface. The first surface is connected with the dielectric medium to constitute an interface. The sensing surface is back to the first surface, for detecting (bio) chemical reaction. A flow cell block has a flow channel for flowing of the sample to the sensing surface. Attenuated total reflection of illuminating light is checked at the interface, to analyze interaction between ligand and analyte as samples. The flow channel includes a first inner surface, disposed opposite to the sensing surface to extend along, for passing the sample to flow between. The first inner surface has a height, defined with reference to the sensing surface, and in a range of 200-500 microns.

摘要翻译： 表面等离子体共振（SPR）测定系统的传感器单元包括透明电介质。 薄膜具有第一表面和感测表面。 第一表面与电介质连接以构成界面。 感测表面回到第一个表面，用于检测（生物）化学反应。 流动池块具有用于将样品流动到感测表面的流动通道。 在界面处检查照明光的衰减全反射，分析配体和分析物之间的相互作用作为样品。 流动通道包括与感测表面相对设置的第一内表面，以沿着该方式延伸以使样品在两者之间流动。 第一内表面具有相对于感测表面限定的高度，并且在200-500微米的范围内。

公开(公告)号：US07453573B2

公开(公告)日：2008-11-18

申请号：US11905029

申请日：2007-09-27

申请人： Nobuhiko Ogura

发明人： Nobuhiko Ogura

IPC分类号： G01J1/00 ,  G01N21/01 ,  B01L3/00

CPC分类号： G01N21/553 ,  G01N2201/1217

摘要： A measuring apparatus and a measuring method are configured to measure a concentration of an organic solvent in a sample solution, measure a concentration of the organic solvent in a concentration control solution, calculate a mixture amount by which the concentration control solution is mixed into the sample solution of a preset amount so that the concentration of the organic solvent in the sample solution is equal to a preset concentration based on the two concentrations, mix the concentration control solution of the calculated mixture amount with the sample solution of the preset amount, supply a mixture solution obtained by the mixing unit to a measurement region in which a bioactive substance is immobilized in advance, and measure an interaction between the bioactive substance and the sample substance in the mixture solution based on a result of measuring a refraction index of a light incident on the measurement region.

摘要翻译： 配置测定装置和测定方法，测定样品溶液中的有机溶剂的浓度，测定浓度对照溶液中的有机溶剂的浓度，算出将浓度对照溶液混合到样品中的混合量 溶液，使得样品溶液中的有机溶剂的浓度基于两种浓度等于预设浓度，将计算出的混合物量的浓度对照溶液与预先设定量的样品溶液混合，供给 通过混合单元获得的混合溶液预先固定有生物活性物质的测量区域，并且基于测量光入射的折射率的结果测量混合溶液中生物活性物质与样品物质之间的相互作用 在测量区域。

公开(公告)号：US07279323B2

公开(公告)日：2007-10-09

申请号：US10351358

申请日：2003-01-27

申请人： Nobuhiko Ogura

发明人： Nobuhiko Ogura

IPC分类号： C12M1/34 ,  C12M3/00

CPC分类号： G01N33/5438 ,  B01J2219/00286 ,  B01J2219/00378 ,  B01J2219/00423 ,  B01J2219/00527 ,  B01J2219/00574 ,  B01J2219/00576 ,  B01J2219/00578 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00644 ,  B01J2219/00653 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/00689 ,  B01J2219/00707 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01L3/5085 ,  C12Q1/6837 ,  G01N33/54366 ,  C12Q2565/607

摘要： A method for conducting a receptor-ligand association reaction includes the steps of dipping a biochemical analysis unit including a substrate formed with a plurality of absorptive regions which contain receptors or ligands and are formed to be spaced apart from each other in a reaction solution containing a ligand or receptor labeled with a labeling substance, simultaneously inserting a plurality of electrodes into all of the plurality of absorptive regions containing the receptors or ligands and sequentially applying a positive voltage to one of the electrodes at a time while other electrodes are grounded, thereby conducting a receptor-ligand association reaction. According to the this method, it is possible to efficiently react a ligand or receptor with receptors or ligands fixed in the plurality of absorptive regions of the biochemical analysis unit and produce biochemical analysis data having an excellent quantitative characteristic with good repeatability.

摘要翻译： 用于进行受体 - 配体缔合反应的方法包括以下步骤：浸渍生物化学分析单元，其包括形成有多个吸收区的底物，所述吸收区包含受体或配体，并形成为在包含受体或配体的反应溶液中彼此间隔开 配体或受体用标记物标记的受体，同时将多个电极插入到包含受体或配体的多个吸收区域的所有多个吸收区域中，并且在其它电极接地时一次在一个电极上顺序施加正电压，由此导通 受体 - 配体缔合反应。 根据该方法，可以使配体或受体与生物化学分析单元的多个吸收区域中固定的受体或配体有效反应，生成具有良好的重复性优异的定量特性的生化分析数据。

公开(公告)号：US20060003395A1

公开(公告)日：2006-01-05

申请号：US11137390

申请日：2005-05-26

申请人： Masayoshi Yamamoto ,  Toshihide Ezoe ,  Nobuhiko Ogura ,  Katsumi Hayashi

发明人： Masayoshi Yamamoto ,  Toshihide Ezoe ,  Nobuhiko Ogura ,  Katsumi Hayashi

IPC分类号： C12Q1/68 ,  G01N33/53 ,  G01N33/537 ,  G01N33/543 ,  C12M1/34

CPC分类号： G01N33/54373 ,  Y10S435/808 ,  Y10S436/805 ,  Y10S436/806

摘要： It is an object of the present invention to provide a method for simultaneously carrying out the detection or measurement of a substance interacting with a physiologically active substance immobilized on a biosensor and the analysis of the effects of the above substance on the biological activity of the physiologically active substance. The present invention provides a measurement method using a biosensor formed by immobilizing a physiologically active substance on a substrate, wherein the detection and measurement of a substance interacting with said physiologically active substance and the measurement of the biological activity of said physiologically active substance are carried out on said single biosensor.

摘要翻译： 本发明的目的是提供一种同时进行与固定在生物传感器上的生理活性物质相互作用的物质的检测或测量的方法，以及上述物质对生理活性的生物学活性的影响的分析 活性物质。 本发明提供一种使用通过将生理活性物质固定在基材上形成的生物传感器的测量方法，其中进行与所述生理活性物质相互作用的物质的检测和测量以及所述生理活性物质的生物活性的测量。 在所述单个生物传感器上。

公开(公告)号：US20050014291A1

公开(公告)日：2005-01-20

申请号：US10890156

申请日：2004-07-14

申请人： Nobuhiko Ogura

发明人： Nobuhiko Ogura

IPC分类号： B01L3/00 ,  C12M1/34 ,  C12Q1/68 ,  C40B60/14 ,  G01N33/543 ,  G01N35/02

CPC分类号： B01L3/5027 ,  B01J2219/00286 ,  B01J2219/00644 ,  B01J2219/00653 ,  B01J2219/0072 ,  B01L2300/0636 ,  B01L2300/0819 ,  B01L2300/0877 ,  B01L2400/0415 ,  C40B60/14 ,  G01N33/5438

摘要： At a stage between a step for subjecting a receptor or a ligand to specific binding with ligands or receptors, each of which has been bound to one of porous adsorptive regions of a biochemical analysis unit, and a step for detecting the receptor or the ligand, which has been specifically bound to at least one of the ligands or at least one of the receptors, by the utilization of the labeling substance, at least one electrode is located such that the electrode is capable of causing an electric current to flow across each of the porous adsorptive regions of the biochemical analysis unit. A voltage is applied to the electrode in order to cause the electric current to flow across each of the porous adsorptive regions of the biochemical analysis unit.

摘要翻译： 在受体或配体与配体或受体的特异性结合的步骤之间的阶段，其各自已经结合生物化学分析单元的多孔吸附区域之一和检测受体或配体的步骤， 其通过利用标记物质特异性地结合至至少一种配体或至少一种受体，至少一个电极被定位成使得电极能够使电流流过每个 生化分析单元的多孔吸附区。 向电极施加电压，以使电流流过生化分析单元的多孔吸附区域。

公开(公告)号：US06781143B2

公开(公告)日：2004-08-24

申请号：US10116701

申请日：2002-04-05

申请人： Hitoshi Shimizu ,  Nobuhiko Ogura

发明人： Hitoshi Shimizu ,  Nobuhiko Ogura

IPC分类号： G03B4200

CPC分类号： G01T1/2014 ,  G01T1/2012 ,  G01T1/2985

摘要： A biochemical analysis data producing method includes the steps of exposing stimulable phosphor layer regions formed in a stimulable phosphor sheet and spaced from each other using standard light, irradiating the stimulable phosphor layer regions with a stimulating ray, detecting stimulated emission to produce correction data for the individual stimulable phosphor layer regions, superposing the stimulable phosphor sheet on a biochemical analysis unit including spot-like regions formed in the same pattern as that of the stimulable phosphor layer regions and selectively containing a radioactive labeling substance, exposing the stimulable phosphor layer regions to the radioactive labeling substance, scanning the stimulable phosphor layer regions with a stimulating ray, detecting stimulated emission to produce biochemical analysis data, and correcting the biochemical analysis data using the correction data. According to this biochemical analysis data producing method, it is possible to produce biochemical analysis data having excellent quantitative characteristics.

摘要翻译： 生物化学分析数据生成方法包括以下步骤：使用标准光曝光形成在可刺激的荧光体片材中的可激发的荧光体层区域，并用刺激的光线照射可刺激的荧光体层区域，检测受激发射以产生校正数据 单独的可刺激荧光体层区域，将可刺激的荧光体片材叠加在生物化学分析单元上，该生物化学分析单元包括以与可刺激的荧光体层区域相同的图案形成的斑点状区域，并选择性地含有放射性标记物质，将可刺激的荧光体层区域暴露于 放射性标记物质，用刺激射线扫描可刺激的荧光体层区域，检测受激发射以产生生化分析数据，以及使用校正数据校正生化分析数据。 根据该生化分析数据生成方法，能够生成具有优异的定量特性的生化分析数据。

公开(公告)号：US5047643A

公开(公告)日：1991-09-10

申请号：US667357

申请日：1991-03-08

申请人： Nobuhiko Ogura

发明人： Nobuhiko Ogura

IPC分类号： G03B42/02 ,  G02B26/12 ,  G21K4/00 ,  H04N1/04 ,  H04N1/06

CPC分类号： H04N1/0664 ,  H04N1/0607 ,  G21K4/00

摘要： A radiation image read-out apparatus comprises a sheet holding device for holding a stimulable phosphor sheet on a cylindrical surface, and a spinner having a deflection mirror for reflecting stimulating rays coming from a semi-transparent mirror to the stimulable phosphor sheet, and a condensing lens which converges the reflected stimulating rays on the stimulable phosphor sheet and which has equal focal lengths with respect to stimulating rays and light emitted by the stimulable phosphor sheet. The deflection mirror and the condensing lens rotate together to scan the stimulating rays on the stimulable phosphor sheet in a main scanning direction, which sheet is moved in a sub-scanning direction. A detection lens converges the emitted light, which has passed through the condensing lens and comes from the semi-transparent mirror. An aperture plate allows only the emitted light after being thus converged to pass therethrough, and a photodetector is positioned at the rear of the aperture plate to detect the emitted light.

摘要翻译： 放射线图像读取装置包括用于将可刺激的荧光体片保持在圆柱形表面上的片材保持装置和具有用于将来自半透明反射镜的刺激光线反射到可刺激的荧光片的偏转镜的旋转器， 将反射的刺激光线会聚在可刺激的荧光体片上并且相对于刺激由可刺激的荧光体片发射的光线和光线具有相等焦距的透镜。 偏转镜和聚光透镜一起旋转，以扫描主扫描方向上的可刺激的荧光体片上的刺激光线，该扫描方向的片材沿副扫描方向移动。 检测透镜会聚通过聚光透镜并从半透明反射镜出射的发射光。 光圈板仅允许发射的光在会聚后穿过其中，并且光电检测器位于孔板的后部以检测发射的光。

公开(公告)号：US08244478B2

公开(公告)日：2012-08-14

申请号：US12942770

申请日：2010-11-09

申请人： Takashi Takahashi ,  Shuta Tomita ,  Tetsuya Mitsudomi ,  Yasushi Yatabe ,  Nobuhiko Ogura ,  Masato Some

发明人： Takashi Takahashi ,  Shuta Tomita ,  Tetsuya Mitsudomi ,  Yasushi Yatabe ,  Nobuhiko Ogura ,  Masato Some

IPC分类号： G06F7/00

CPC分类号： C12Q1/6886 ,  C12Q2600/118 ,  C12Q2600/158

摘要： The present invention provides a method of confirming the gene expression, useful in the decision of a five year survival rate of a patient with lung cancer and the use of a DNA probe kit in the method. A method useful in the decision of a survival rate of a patient with non-small cell lung cancer comprising confirming the expression strength of at least one gene in lung cancer tissues isolated from the patient.

摘要翻译： 本发明提供一种确认基因表达的方法，该方法可用于决定患者肺癌的5年生存率，并在该方法中使用DNA探针试剂盒。 用于决定患有非小细胞肺癌的患者存活率的方法，其包括确认从患者分离的肺癌组织中至少一种基因的表达强度。