SYSTEM AND METHODS OF LOG-SCALE CONCENTRATION GRADIENTS
    5.
    发明申请
    SYSTEM AND METHODS OF LOG-SCALE CONCENTRATION GRADIENTS 失效
    记录浓度梯度的系统和方法

    公开(公告)号:US20130164770A1

    公开(公告)日:2013-06-27

    申请号:US13305645

    申请日:2011-11-28

    IPC分类号: C12Q1/37

    摘要: Devices and methods use an integrated microfluidic system that has the capability of realizing a wide range of accurate dilutions in a logarithmic way through semi-direct dilution of samples inside a chip. The device for dose response analysis is able to contain a first fluid source on a microfluidic chip, wherein the first fluid source comprises a drug, a second fluid source on the microfluidic chip, a mixing area on the microfluidic chip fluidically coupling with the first and the second fluidic source, and a detection area coupling with the mixing area for drug information detection using a detection system.

    摘要翻译: 设备和方法使用集成的微流体系统,其具有通过半直接稀释芯片内的样品以对数方式实现宽范围的精确稀释的能力。 用于剂量反应分析的装置能够在微流体芯片上包含第一流体源,其中第一流体源包括药物,微流体芯片上的第二流体源,微流体芯片上与第一和第二流体源流体耦合的混合区域 第二流体源,以及使用检测系统与药物信息检测用混合区域连接的检测区域。

    Microfluidic nucleic acid analysis
    7.
    发明授权
    Microfluidic nucleic acid analysis 有权
    微流控核酸分析

    公开(公告)号:US08871446B2

    公开(公告)日:2014-10-28

    申请号:US10678946

    申请日:2003-10-02

    IPC分类号: C12Q1/68 C12P19/34 B01L3/00

    摘要: Nucleic acid from cells and viruses sampled from a variety of environments may purified and expressed utilizing microfluidic techniques. In accordance with one embodiment of the present invention, individual or small groups of cells or viruses may be isolated in microfluidic chambers by dilution, sorting, and/or segmentation. The isolated cells or viruses may be lysed directly in the microfluidic chamber, and the resulting nucleic acid purified by exposure to affinity beads. Subsequent elution of the purified nucleic acid may be followed by ligation and cell transformation, all within the same microfluidic chip. In one specific application, cell isolation, lysis, and nucleic acid purification may be performed utilizing a highly parallelized microfluidic architecture to construct gDNA and cDNA libraries.

    摘要翻译: 来自从各种环境取样的细胞和病毒的核酸可以利用微流体技术纯化和表达。 根据本发明的一个实施方案,可通过稀释,分选和/或分割在微流体室中分离单个或小组的细胞或病毒。 分离的细胞或病毒可以直接在微流体室中裂解,并且通过暴露于亲和珠来纯化得到的核酸。 随后洗脱纯化的核酸之后可以连接和细胞转化,全部在相同的微流体芯片内。 在一个具体应用中,细胞分离,裂解和核酸纯化可以使用高度并行的微流体结构来构建gDNA和cDNA文库。

    System and methods of log-scale concentration gradients
    8.
    发明授权
    System and methods of log-scale concentration gradients 失效
    对数刻度浓度梯度的系统和方法

    公开(公告)号:US08771611B2

    公开(公告)日:2014-07-08

    申请号:US13305645

    申请日:2011-11-28

    IPC分类号: G01N15/06

    摘要: Devices and methods use an integrated microfluidic system that has the capability of realizing a wide range of accurate dilutions in a logarithmic way through semi-direct dilution of samples inside a chip. The device for dose response analysis is able to contain a first fluid source on a microfluidic chip, wherein the first fluid source comprises a drug, a second fluid source on the microfluidic chip, a mixing area on the microfluidic chip fluidically coupling with the first and the second fluidic source, and a detection area coupling with the mixing area for drug information detection using a detection system.

    摘要翻译: 设备和方法使用集成的微流体系统,其具有通过半直接稀释芯片内的样品以对数方式实现宽范围的精确稀释的能力。 用于剂量反应分析的装置能够在微流体芯片上包含第一流体源,其中第一流体源包括药物,微流体芯片上的第二流体源,微流体芯片上与第一和第二流体源流体耦合的混合区域 第二流体源,以及使用检测系统与药物信息检测用混合区域连接的检测区域。