公开(公告)号：US20070068964A1

公开(公告)日：2007-03-29

申请号：US11524948

申请日：2006-09-22

申请人： Takeo Tanaami ,  Hidetoshi Aoki ,  Saya Satou ,  Takeyuki Mogi

发明人： Takeo Tanaami ,  Hidetoshi Aoki ,  Saya Satou ,  Takeyuki Mogi

IPC分类号： B65D35/22 ,  B65D35/28 ,  B67D5/52 ,  B67D5/60

CPC分类号： B01L3/502707 ,  B01F5/0641 ,  B01F5/0645 ,  B01F5/0693 ,  B01F5/0696 ,  B01F13/0059 ,  B01F15/0201 ,  B01F15/025 ,  B01J19/0093 ,  B01J2219/00783 ,  B01J2219/00833 ,  B01J2219/00835 ,  B01J2219/0086 ,  B01J2219/00889 ,  B01J2219/00891 ,  B01L3/5027 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2400/0481

摘要： A chemical reaction cartridge enabling chemical reaction to be stably and safely carried out therein, and a method of using the same are provided. A chemical reaction cartridge is provided with a substrate, and an elastic member 2 overlaid on the substrate. A recess of a predetermined shape, depressed toward the top surface of the elastic member is formed in the back surface of the elastic member. The recess creates space between the substrate, and the elastic member, thereby making up a well, for holding the main ingredient of the epoxy adhesive, a flow path communicating with the well, a well for holding a curing agent for the epoxy adhesive, a flow path communicating with the well, a mixing part for mixing the main ingredient with the curing agent, and a discharge path for discharging a mixture. While the chemical reaction cartridge is not in use as yet, the flow paths, and the discharge path are closed, so that the wells are, in effect, in a hermetically sealed condition. The mixing part is made up of a plurality of flow paths intersecting each other. The respective starting ends of the flow paths are each linked with the flow path, or the flow path, and the respective terminals of the flow paths are linked with the discharge path. The terminal of the discharge path communicates with the outside via an end face of the cartridge.

摘要翻译： 能够使化学反应稳定且安全地进行的化学反应筒及其使用方法。 化学反应盒设置有基板和覆盖在基板上的弹性部件2。 在弹性部件的背面形成有朝向弹性部件的上表面凹陷的规定形状的凹部。 凹部在基板和弹性部件之间形成空间，从而构成一个孔，用于保持环氧粘合剂的主要成分，与该孔连通的流路，用于保持环氧粘合剂的固化剂的孔， 与井连通的流路，用于混合主成分与固化剂的混合部以及排出混合物的排出路径。 虽然化学反应盒尚未使用，但是流动路径和排放路径是关闭的，因此实际上这些井处于气密密封状态。 混合部分由彼此相交的多个流路组成。 流路的各个起始端各自与流路或流路连通，流路的各端子与排出路径连结。 排出路径的端子经由筒的端面与外部连通。

公开(公告)号：US07789270B2

公开(公告)日：2010-09-07

申请号：US11524948

申请日：2006-09-22

申请人： Takeo Tanaami ,  Hidetoshi Aoki ,  Saya Satou ,  Takeyuki Mogi

发明人： Takeo Tanaami ,  Hidetoshi Aoki ,  Saya Satou ,  Takeyuki Mogi

IPC分类号： B65D35/22

CPC分类号： B01L3/502707 ,  B01F5/0641 ,  B01F5/0645 ,  B01F5/0693 ,  B01F5/0696 ,  B01F13/0059 ,  B01F15/0201 ,  B01F15/025 ,  B01J19/0093 ,  B01J2219/00783 ,  B01J2219/00833 ,  B01J2219/00835 ,  B01J2219/0086 ,  B01J2219/00889 ,  B01J2219/00891 ,  B01L3/5027 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2400/0481

摘要： A chemical reaction cartridge enabling chemical reaction to be stably and safely carried out therein, and a method of using the same are provided. A chemical reaction cartridge is provided with a substrate, and an elastic member 2 overlaid on the substrate. A recess of a predetermined shape, depressed toward the top surface of the elastic member is formed in the back surface of the elastic member. The recess creates space between the substrate, and the elastic member, thereby making up a well, for holding the main ingredient of the epoxy adhesive, a flow path communicating with the well, a well for holding a curing agent for the epoxy adhesive, a flow path communicating with the well, a mixing part for mixing the main ingredient with the curing agent, and a discharge path for discharging a mixture. While the chemical reaction cartridge is not in use as yet, the flow paths, and the discharge path are closed, so that the wells are, in effect, in a hermetically sealed condition. The mixing part is made up of a plurality of flow paths intersecting each other. The respective starting ends of the flow paths are each linked with the flow path, or the flow path, and the respective terminals of the flow paths are linked with the discharge path. The terminal of the discharge path communicates with the outside via an end face of the cartridge.

摘要翻译： 能够使化学反应稳定且安全地进行的化学反应筒及其使用方法。 化学反应盒设置有基板和覆盖在基板上的弹性部件2。 在弹性部件的背面形成有朝向弹性部件的上表面凹陷的规定形状的凹部。 凹部在基板和弹性部件之间形成空间，从而构成一个孔，用于保持环氧粘合剂的主要成分，与该孔连通的流路，用于保持环氧粘合剂的固化剂的孔， 与井连通的流路，用于混合主成分与固化剂的混合部以及排出混合物的排出路径。 虽然化学反应盒尚未使用，但是流动路径和排放路径是关闭的，因此实际上这些井处于气密密封状态。 混合部分由彼此相交的多个流路组成。 流路的各个起始端各自与流路或流路连通，流路的各端子与排出路径连结。 排出路径的端子经由筒的端面与外部连通。

公开(公告)号：US07978327B2

公开(公告)日：2011-07-12

申请号：US12365280

申请日：2009-02-04

申请人： Takeo Tanaami ,  Hidetoshi Aoki ,  Saya Satou ,  Yumiko Sugiyama

发明人： Takeo Tanaami ,  Hidetoshi Aoki ,  Saya Satou ,  Yumiko Sugiyama

IPC分类号： G01B9/08

CPC分类号： G01N21/6458

摘要： There is provided a molecularity measurement instrument capable of working out the number of molecules in a sample by comparing a measured value of a light quantity with a theoretical light quantity per a single molecule, and a molecularity measurement method using the same. The molecularity of the sample is quantitatively estimated on the basis of a light quantity having correlation with the molecularity. The molecularity measurement method comprises the step of working out a theoretical light quantity per a single molecule, the step of measuring a light quantity of the sample by use of an image detector, and the step of working out the molecularity of the sample on the basis of a ratio of the light quantity of the sample to the theoretical light quantity as worked out.

摘要翻译： 提供一种能够通过将光量的测定值与单一分子的理论光量进行比较来求出样品中的分子数的分子量测定仪和使用其的分子量测定方法。 基于与分子相关的光量定量地估计样品的分子量。 分子量测定方法包括：通过使用图像检测器测定单个分子的理论光量的步骤，测定样品的光量的步骤，以及基于样品的分子量的步骤 测定样品的光量与理论光量的比率。

公开(公告)号：US07858045B2

公开(公告)日：2010-12-28

申请号：US11528535

申请日：2006-09-28

申请人： Takeo Tanaami ,  Saya Satou ,  Hisao Katakura

发明人： Takeo Tanaami ,  Saya Satou ,  Hisao Katakura

IPC分类号： B01L3/00

CPC分类号： B01L3/5027 ,  B01L7/525 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/087 ,  B01L2300/123 ,  B01L2400/0421 ,  B01L2400/0481 ,  G01N27/447 ,  Y10T436/25375 ,  Y10T436/255 ,  Y10T436/2575

摘要： A cartridge adaptable to various measurement forms is provided. A sample is injected into a well via an injection path. When a roller while kept pressed into contact with the cartridge is rotated rightward, the sample held in the well, and liquid solvent held in a well are caused to reach a well via flow paths, respectively, thereby mixing the sample with the liquid solvent. A mixed liquid is branched into flow paths, respectively, to thereby reach wells, respectively, and reagents pre-held in wells, respectively, are caused to flow into the wells, respectively. Next, temperatures in the wells, respectively, are controlled, thereby executing DNA amplification. Then, PCR by-products as amplified in the wells, respectively, are transferred as far as a position in the vicinity of an electrode via flow paths. Subsequently, a voltage is applied across the electrode as a negative electrode, and an electrode as a positive electrode via a drawing electrode and a drawing electrode, to thereby cause the PCR by-products to undergo electrophoresis.

摘要翻译： 提供适用于各种测量形式的盒式磁带。 样品通过注射路径注入孔中。 当保持按压与筒接触的辊子向右旋转时，将样品保持在井中，并且使保持在孔中的液体溶剂分别通过流动路径到达井，从而将样品与液体溶剂混合。 分别将混合液体分支成流路，从而分别到达井，分别将预先保存在井中的试剂流入孔中。 接下来，分别控制孔中的温度，从而进行DNA扩增。 然后，分别在孔中扩增的PCR副产物通过流路被转移到电极附近的位置。 随后，通过拉伸电极和拉伸电极将作为负极的电极和作为正极的电极施加电压，从而使PCR副产物进行电泳。

公开(公告)号：US20080038164A1

公开(公告)日：2008-02-14

申请号：US11832297

申请日：2007-08-01

申请人： Saya Satou ,  Takeo Tanaami

发明人： Saya Satou ,  Takeo Tanaami

IPC分类号： B01J19/00 ,  F17D1/08

CPC分类号： B01L3/502 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/0887 ,  B01L2400/0481 ,  B01L2400/0605 ,  Y10T137/0391

摘要： A biochemical reaction apparatus used to carry out a chemical reaction of fluid includes a cartridge including a container which is at least partially structured with an elastic body, the container including inside thereof a plurality of chambers to contain the fluid and flow passages to connect the plurality of chambers and rollers to apply an external force to the elastic body and deform the elastic body to move the fluid in the flow passages or the chambers by rotationally moving on a front surface of the elastic body while the roller contacts with the front surface of the elastic body, and in a cross-sectional shape of the roller, which is perpendicular to a roller shaft, at least not less than three corners are included, and the cross-sectional shape is a shape in which sides between the corners are equal in length.

摘要翻译： 用于进行流体化学反应的生物化学反应装置包括一个盒，该盒包括至少部分地由弹性体构成的容器，该容器在其内部包括多个室，以容纳流体和流动通道以连接多个 的腔室和辊子，以向弹性体施加外力，并使弹性体变形，以通过在弹性体的前表面上旋转移动来使流动通道或腔室中的流体移动，同时辊子与弹性体的前表面接触 弹性体，并且在垂直于辊轴的辊的横截面形状中至少包括三个角，并且横截面形状是角部之间的边相等的形状 长度。

公开(公告)号：US09061280B2

公开(公告)日：2015-06-23

申请号：US12656770

申请日：2010-02-16

申请人： Takeo Tanaami ,  Hisao Katakura ,  Saya Satou ,  Naoki Seki

发明人： Takeo Tanaami ,  Hisao Katakura ,  Saya Satou ,  Naoki Seki

IPC分类号： B01L3/00 ,  B01J19/00 ,  G01N33/18

CPC分类号： B01L3/502738 ,  B01J19/0093 ,  B01J2219/00783 ,  B01J2219/00833 ,  B01J2219/00889 ,  B01J2219/00891 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502746 ,  B01L3/505 ,  B01L2200/0689 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2300/14 ,  B01L2300/1822 ,  B01L2400/0481 ,  B01L2400/0655 ,  G01N33/18

摘要： The present invention makes it possible to realize a chemical reaction cartridge, which enables prescribed protocols to be achieved easily without differences among operators. The cartridge is sealed and disposable, and has a safe structure against viruses or dangerous drugs. This cartridge is intended to be used for performing chemical reaction of samples. The cartridge comprises a container formed of a rigid substrate and an elastic body, wherein two or more chambers linked or arranged to be able to be linked through flow paths are formed in the container. The flow paths, the chambers or both are partially closed by applying an external force to the elastic body from outside the container, thereby being able to move or block fluid substance in said flow paths or chambers.

摘要翻译： 本发明使得可以实现能够容易地实现规定的协议而无需操作者之间的差异的化学反应盒。 药筒是密封和一次性的，并且具有防止病毒或危险药物的安全结构。 该墨盒旨在用于进行样品的化学反应。 盒包括由刚性基底和弹性体形成的容器，其中通过流动路径连接或布置成能够连接的两个或多个腔室形成在容器中。 通过从容器的外部向弹性体施加外力，从而能够移动或阻挡所述流动路径或腔室中的流体物质，从而部分地封闭流路，室或两者。

公开(公告)号：US20110280776A1

公开(公告)日：2011-11-17

申请号：US13190680

申请日：2011-07-26

申请人： Takeo Tanaami ,  Hisao Katakura ,  Nobuyuki Kakuryu ,  Saya Satou

发明人： Takeo Tanaami ,  Hisao Katakura ,  Nobuyuki Kakuryu ,  Saya Satou

IPC分类号： B01L3/00 ,  B65D8/04

CPC分类号： B01L3/523 ,  B01L3/5027 ,  B01L2200/16 ,  B01L2300/0867 ,  B01L2400/0481 ,  B01L2400/0683 ,  Y10T428/13

摘要： There are provided a capsule and a chemical reaction cartridge capable of properly handling reagents and so forth. The capsule is formed by laminating two dome-shaped films together at the peripheries thereof. The films are formed by subjecting heat sealable films, to which aluminum vapor deposition is applied, to a drawing process. The films are made of a material (easy-peel material), which can be varied in seal strength by a heating temperature and are thermally welded together at an adhesion area at the peripheries thereof. Thereafter, contents such as reagents and so forth are filled up inside the capsule via a filling section.

摘要翻译： 提供了能够适当地处理试剂等的胶囊和化学反应盒。 胶囊通过在其周边层叠两个圆顶形膜而形成。 通过将施加了铝蒸气沉积的可热封膜进行拉伸加工形成膜。 膜由材料（易剥离材料）制成，其可以通过加热温度改变密封强度，并且在其周边的粘合区域处热焊接在一起。 此后，通过填充部将诸如试剂等的内容物填充在胶囊内。

公开(公告)号：US07781204B2

公开(公告)日：2010-08-24

申请号：US11519809

申请日：2006-09-13

申请人： Saya Satou ,  Takeo Tanaami

发明人： Saya Satou ,  Takeo Tanaami

IPC分类号： C12M1/34 ,  C12M3/00

CPC分类号： G01N21/6428 ,  G01N21/6452 ,  G01N33/543 ,  G01N33/582

摘要： There is provided a biochip capable of quantitatively grasping hybridization efficiency. In the biochip comprising a plurality of probe sites with target molecules bonded thereto, respectively, the probe sites disposed thereon, the marker sites with a known number of fluorescent molecules bonded thereto, respectively, are disposed. With the biochip, the number of the fluorescent molecules bonded to the respective marker sites is already known, so that respective hybridization efficiencies at the probe sites can be quantitatively grasped by comparing respective intensity of fluorescent light of the probe sites with an intensity of fluorescent light of the marker sites. The fluorescent molecule may be bonded to the respective marker sites when the biochip is formed, or the respective marker sites may be made up such that the fluorescent molecule of a predetermined molecular weight is bonded thereto by a predetermined processing applied to the biochip. The respective marker sites may be formed by use of a biopolymer of the same species as that for the respective probe sites.

摘要翻译： 提供了能够定量地掌握杂交效率的生物芯片。 在包含分别与靶分子结合的多个探针位点的生物芯片中，分别设置有探针位置，分别与已知数目的荧光分子结合的标记位点。 利用生物芯片，结合到各个标记位点的荧光分子的数目是已知的，因此通过将探针部位的荧光的各自的强度与荧光强度进行比较，可以定量地掌握探针位置处的各杂交效率 的标记位点。 当形成生物芯片时，荧光分子可以结合到各个标记位点，或者可以通过预定的处理应用于生物芯片将预定分子量的荧光分子结合到各个标记位点。 各个标记位点可以通过使用与各个探针位点相同种类的生物聚合物形成。

公开(公告)号：US20060275813A1

公开(公告)日：2006-12-07

申请号：US11446225

申请日：2006-06-05

申请人： Takeo Tanaami ,  Hisao Katakura ,  Saya Satou

发明人： Takeo Tanaami ,  Hisao Katakura ,  Saya Satou

IPC分类号： C12Q1/68 ,  C12M1/34

CPC分类号： B01L3/502715 ,  B01L7/52 ,  B01L2300/0636 ,  B01L2300/0681 ,  B01L2300/0825 ,  B01L2300/087 ,  B01L2300/0877 ,  B01L2400/0421 ,  B01L2400/0481

摘要： The cartridge for chemical reaction according to the invention is a cartridge for chemical reaction that causes chemical reaction by feeding an internal liquid by way of deformation caused by external forces. The entirety of a target molecule detecting chip is housed in the cartridge for chemical reaction. According to the cartridge for chemical reaction, the entire target molecule detecting chip is housed in the cartridge for chemical reaction so that it supports various forms of target molecule detecting chips. The target molecule detecting chip may be a penetration type chip that allows a solution including target molecules to penetrate the target molecule detecting chip. In this case, the solution may pass inside the penetration type chip by way of deformation of the cartridge for chemical reaction caused by external forces applied to the cartridge for chemical reaction.

摘要翻译： 根据本发明的用于化学反应的药筒是用于通过由外力引起的变形而通过供给内部液体而引起化学反应的化学反应的药筒。 靶分子检测芯片的整体容纳在用于化学反应的盒中。 根据用于化学反应的盒，整个靶分子检测芯片被容纳在用于化学反应的盒中，从而它支持各种形式的靶分子检测芯片。 靶分子检测芯片可以是允许包含靶分子的溶液穿透靶分子检测芯片的穿透型芯片。 在这种情况下，溶液可以通过由施加到用于化学反应的盒的外力引起的用于化学反应的盒的变形而穿过穿透型芯片。

公开(公告)号：US07854897B2

公开(公告)日：2010-12-21

申请号：US10833158

申请日：2004-04-28

申请人： Takeo Tanaami ,  Hisao Katakura ,  Saya Satou ,  Naoki Seki

发明人： Takeo Tanaami ,  Hisao Katakura ,  Saya Satou ,  Naoki Seki

IPC分类号： B01L3/00

CPC分类号： B01L3/502738 ,  B01J19/0093 ,  B01J2219/00783 ,  B01J2219/00833 ,  B01J2219/00889 ,  B01J2219/00891 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502746 ,  B01L3/505 ,  B01L2200/0689 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2300/14 ,  B01L2300/1822 ,  B01L2400/0481 ,  B01L2400/0655 ,  G01N33/18

摘要： The present invention describes a disposable chemical reaction cartridge comprising a container formed of a rigid substrate and an elastic body, wherein two or more chambers are linked or configured to be linked through flow paths, and wherein the flow paths, the chambers or both, can be partially closed by applying an external force to the elastic body from outside of the container, to move or block fluids in the flow paths or chambers. The cartridge has a structure protected against viruses or dangerous drugs and facilitates foolproof implementation of prescribed protocols for the samples to be tested.

摘要翻译： 本发明描述了一种一次性化学反应盒，其包括由刚性基材和弹性体形成的容器，其中两个或更多个室被连接或构造成通过流动路径连接，并且其中流动路径，室或两者可以 通过从容器的外部向弹性体施加外力来移动或阻挡流动路径或腔室中的流体而部分闭合。 药筒具有防病毒或危险药物的结构，并且有助于对要测试的样品的规定的协议的实施。