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    Degree of hybridization detection method
    4.
    发明授权
    Degree of hybridization detection method 失效
    杂交检测方法

    公开(公告)号：US06775621B1

    公开(公告)日：2004-08-10

    申请号：US09459712

    申请日：1999-12-13

    申请人： Noriko Yurino ,  Kenji Yamamoto ,  Toshiaki Ito ,  Toshimasa Watanabe

    发明人： Noriko Yurino ,  Kenji Yamamoto ,  Toshiaki Ito ,  Toshimasa Watanabe

    IPC分类号： G01N3348

    CPC分类号： C12Q1/6837 ,  C12Q2545/114 ,  C12Q2545/101 ,  C12Q2563/107 ,  C12Q2565/102

    摘要： The present invention provides a hybridization detection method capable of quantitatively determining the degree of the hybridization between a sample biopolymer and a probe biopolymer. In the method, the amount of a fluorescently labeled probe immobilized on a substrate as a spot is quantitatively determined by determining the fluorescence emitted from a fluorescent material labeling the probe, and the amount of a fluorescently labeled sample hybridized to the probe is quantitatively determined by determining the fluorescence emitted from the fluorescent material labeling the sample. The difference between the amount of the probe and the amount of the sample is normalized with the amount of the probe. Based on the normalized value, the amount of the sample hybridized to the probe can be determined relative to the amount of the probe spotted on the substrate.

    摘要翻译： 本发明提供能够定量测定样品生物聚合物和探针生物聚合物之间的杂交程度的杂交检测方法。 在该方法中，通过测定从标记探针的荧光物质发出的荧光来定量测定固定在基板上的荧光标记探针作为斑点的量，并且通过用探针杂交的荧光标记的样品的量定量地测定 确定从标记样品的荧光材料发出的荧光。 探针的量与样品量之间的差异用探针的量进行归一化。 基于归一化值，可以相对于在基板上点样的探针的量来确定与探针杂交的样品的量。

    Method and device for detecting hybridization reaction
    5.
    发明授权
    Method and device for detecting hybridization reaction 失效
    检测杂交反应的方法和装置

    公开(公告)号：US06589740B2

    公开(公告)日：2003-07-08

    申请号：US09802804

    申请日：2001-03-09

    申请人： Motonao Nakao ,  Kenji Yamamoto ,  Junji Yoshii ,  Katsuya Mizuno

    发明人： Motonao Nakao ,  Kenji Yamamoto ,  Junji Yoshii ,  Katsuya Mizuno

    IPC分类号： C12Q168

    CPC分类号： C12Q1/6837 ,  B01L7/52 ,  B01L2300/0819 ,  C12Q2563/107 ,  C12Q2527/107

    摘要： The present invention detects and quantities only specific hybridization bindings. A biochip 10 which is spotted with a plurality of probe biopolymers is accommodated in a container 20 into which a washing solution is supplied from a liquid supplying unit 23. A heat block 31 controls the temperature of the biochip according to a predetermined time pattern. A pickup unit 55 picks up an image of the spot surface of the biochip at predetermined pickup intervals. The plurality of images picked up with the pickup unit are stored in a computer 40. By analyzing the images for individual spots, hybridization can be detected with high reliability for every spots without being influenced by optimal hybridization temperatures which differ depending upon the types of probes on the spots.

    摘要翻译： 本发明仅检测和量化特异性杂交结合。 用多个探针生物聚合物点样的生物芯片10容纳在从液体供应单元23供应洗涤溶液的容器20中。加热块31根据预定的时间模式控制生物芯片的温度。 拾取单元55以预定的取样间隔拾取生物芯片的光斑表面的图像。 用拾取单元拾取的多个图像被存储在计算机40中。通过分析各个斑点的图像，可以以高可靠性检测每个斑点的杂交，而不受根据探针类型而不同的最佳杂交温度的影响 在斑点上。

    Apparatus for reading a luminescence pattern of a sample
    6.
    发明授权
    Apparatus for reading a luminescence pattern of a sample 失效
    用于读取样品的发光图案的装置

    公开(公告)号：US5991030A

    公开(公告)日：1999-11-23

    申请号：US66875

    申请日：1998-04-28

    申请人： Kenji Yamamoto ,  Hisanori Nasu ,  Toshimasa Watanabe ,  Yuuji Tsukamoto ,  Tateo Kondou ,  Takehiko Nishida ,  Hitoshi Fujimiya ,  Noriko Yurino

    发明人： Kenji Yamamoto ,  Hisanori Nasu ,  Toshimasa Watanabe ,  Yuuji Tsukamoto ,  Tateo Kondou ,  Takehiko Nishida ,  Hitoshi Fujimiya ,  Noriko Yurino

    IPC分类号： G01N27/447 ,  G01R9/02 ,  F21V9/16

    CPC分类号： G01N27/44721

    摘要： The apparatus for reading a luminescence pattern is equipped with a stage on which the sample to be read is mounted. A photoreceptor disk is installed in the lower part of the stage and scans and condenses light from the luminescent pattern of the sample at positions determined by the rotation of a rotary plate. A transport mechanism causes the stage and the photoreceptor disk to undergo relative motion. An optical guide part guides the light from the luminescent pattern to a photoelectric converter. The optical guide part may include mirrors or one or more optical fibers. The photoelectric converter converts the light into an electrical signal. A controller controls how scanning is performed by the photoreceptor disk and transport mechanism and generates a read scanning position signal. A data processor performs data processing by converting the electrical signal into a digital signal and obtains a scanning position signal from the controller. Additionally, a laser light source may be used to shine laser light onto an electrophoresis gel of the sample.

    摘要翻译： 用于读取发光图案的装置装备有待安装样品的阶段。 感光盘安装在舞台的下部，并且通过旋转板旋转确定的位置扫描并冷凝来自样品的发光图案的光。 传送机构使舞台和感光盘发生相对运动。 光导部分将光从发光图案引导到光电转换器。 光导部分可以包括反射镜或一个或多个光纤。 光电转换器将光转换成电信号。 控制器控制感光盘和传送机构的扫描方式，并生成读取扫描位置信号。 数据处理器通过将电信号转换为数字信号并从控制器获得扫描位置信号来执行数据处理。 此外，可以使用激光源将激光照射到样品的电泳凝胶上。

    Scanning apparatus
    7.
    发明授权
    Scanning apparatus 失效
    扫描仪

    公开(公告)号：US5953133A

    公开(公告)日：1999-09-14

    申请号：US641424

    申请日：1996-05-01

    申请人： Hitoshi Fujimiya ,  Kenji Yamamoto ,  Toshiaki Ito ,  Hisanori Nasu

    发明人： Hitoshi Fujimiya ,  Kenji Yamamoto ,  Toshiaki Ito ,  Hisanori Nasu

    IPC分类号： G01N27/447 ,  G02F1/055 ,  G06T1/00 ,  H04N1/04 ,  G01B11/00 ,  G01N21/00 ,  H01L31/14

    CPC分类号： G01N27/44721

    摘要： A scanning apparatus for scanning and reading a luminescent pattern of a sample in a flat-plate shape, having a placing member for placing the sample as an object for reading; a light condensing member for condensing light emitted from the luminescent pattern of the sample; a movement member for moving the light condensing member relative to the placing member; a light receiving member for dividing the light of the luminescent pattern of the sample condensed by the light condensing member into predetermined segments and receiving the light by scanning the light from the segments in a one-dimensional way; a photoelectrical conversion member for converting optical signals of the light received by the light receiving member into electrical signals; a control member for controlling a scan by the light receiving member in accordance with the electrical signals from the photoelectrical conversion member; and a data processing member for converting the electrical signals from the photoelectrical conversion member into digital signals and reconfiguring an image from the optical signals of the light from the segment received selectively in a one-dimensional way. The scanning apparatus can likewise scan and read a non-luminous or less luminous pattern of a sample by allowing light from a plane light source to transmit through the pattern of the sample.

    摘要翻译： 一种用于扫描和读取平板形状的样品的发光图案的扫描装置，具有用于放置样品作为读取对象的放置构件; 用于聚集从样品的发光图案发出的光的聚光部件; 用于使所述聚光部件相对于所述放置部件移动的移动部件; 光接收部件，用于将通过聚光部件冷凝的样品的发光图案的光分成预定的段，并以一维方式扫描来自该段的光; 光电转换构件，用于将由光接收构件接收的光的光信号转换为电信号; 控制构件，用于根据来自光电转换构件的电信号控制受光构件的扫描; 以及数据处理部件，用于将来自光电转换部件的电信号转换成数字信号，并且以一维方式从选择性地接收来自光盘的光的光信号重构图像。 扫描装置同样可以通过允许来自平面光源的光透过样品的图案来扫描和读取样品的非发光或更少的发光图案。

    Electrocatalyst
    8.
    发明授权
    Electrocatalyst 有权

    公开(公告)号：US11901568B2

    公开(公告)日：2024-02-13

    申请号：US17653400

    申请日：2022-03-03

    申请人： Ryo Shimizu ,  Kumiko Nomura ,  Tomohiro Takeshita ,  Shu Miyasaka ,  Kenji Yamamoto

    发明人： Ryo Shimizu ,  Kumiko Nomura ,  Tomohiro Takeshita ,  Shu Miyasaka ,  Kenji Yamamoto

    IPC分类号： H01M4/00 ,  H01M4/92 ,  H01M4/88 ,  C22C5/04

    CPC分类号： H01M4/926 ,  H01M4/8842 ,  C22C5/04

    摘要： To provide an electrocatalyst for fuel cells, which is configured to ensure both the initial performance and durability of fuel cells. An electrocatalyst for fuel cells, wherein the electrocatalyst comprises a carbon support including a mesopore and a catalyst alloy supported on the carbon support, and the catalyst alloy is a catalyst alloy of platinum and a transition metal; wherein the mesopore includes at least one throat; wherein an average effective diameter of the at least one throat is 1.8 nm or more and less than 3.2 nm; and wherein a transition metal ratio of the catalyst alloy supported on a deeper-side region than the at least one throat, is lower than the transition metal ratio of the catalyst alloy supported on a nearer-side region than the at least one throat.

    Gear spindle and oil seal used therein
    10.
    发明授权
    Gear spindle and oil seal used therein 有权

    公开(公告)号：US10016798B2

    公开(公告)日：2018-07-10

    申请号：US14424582

    申请日：2012-08-31

    申请人： Kenji Yamamoto

    发明人： Kenji Yamamoto

    IPC分类号： F16D3/18 ,  B21B35/14 ,  F16D3/16 ,  F16D3/10 ,  F16D3/84 ,  F16J15/52

    CPC分类号： B21B35/147 ,  F16D3/10 ,  F16D3/16 ,  F16D3/185 ,  F16D3/845 ,  F16D2300/06 ,  F16J15/525

    摘要： In a gear spindle, outer cylinder gear sections (11) are each integrally formed on an inner peripheral surface of a spindle outer cylinder and inner cylinder gear sections (14) are each integrally formed on an outer peripheral surface of a spindle inner cylinder (13). An oil seal (27) that seals in the lubricating oil (20) for each of the aforementioned gear sections includes, a seal body (29) having a channel-shaped cross section and interposed in the peripheral gap between the inner peripheral surface of the spindle outer cylinder and the outer peripheral surface of the spindle inner cylinder (13), and a seal mounting member (30) that includes a band, a spring, or the like that tightens and fixes the seal body to the outer peripheral surface of the spindle inner cylinder (13) to allow expansion of the seal body in the axial direction in the aforementioned peripheral gap.