公开(公告)号：US11890339B2

公开(公告)日：2024-02-06

申请号：US17261828

申请日：2019-08-05

申请人： The United States of America, as represented by the Secretary, Department of Health and Human Services ,  Trustees of Dartmouth College

发明人： Barney Graham ,  Rebecca Loomis ,  Guillaume Stewart-Jones ,  John Mascola ,  Jason McLellan

IPC分类号： A61K39/155 ,  A61P31/14 ,  C07K14/005 ,  A61K39/00

CPC分类号： A61K39/155 ,  A61P31/14 ,  C07K14/005 ,  A61K2039/70 ,  C07K2319/03 ,  C12N2760/18222 ,  C12N2760/18223 ,  C12N2760/18234

摘要： Embodiments of immunogens comprising a recombinant Nipah virus (NiV) F ectodomain trimer stabilized in a prefusion conformation are provided. Also provided are embodiments of immunogens comprising chimeric proteins comprising the recombinant NiV F ectodomain trimer and one or more G ectodomains, a multimer of NiV G ectodomains, and protein nanoparticles comprising the recombinant NiV F ectodomain trimer or an NiV G ectodomain. Also disclosed are nucleic acids encoding the immunogens and methods of their production. Methods for inducing an immune response in a subject by administering a disclosed immunogen to the subject are also provided. In some embodiments, the immune response treats or inhibits NiV infection in a subject.

公开(公告)号：US10960070B2

公开(公告)日：2021-03-30

申请号：US16344774

申请日：2017-10-25

申请人： The United States of America, as represented by the Secretary, Department of Health and Human Services ,  The Scripps Research Institute ,  Trustees of Dartmouth College

发明人： Barney Graham ,  Jason McLellan ,  Andrew Ward ,  Robert Kirchdoerfer ,  Christopher Cottrell ,  Michael Gordon Joyce ,  Masaru Kanekiyo ,  Nianshuang Wang ,  Jesper Pallesen ,  Hadi Yassine ,  Hannah Turner ,  Kizzmekia Corbett

IPC分类号： A61K39/215 ,  C12N7/00 ,  A61P31/14 ,  C07K14/005

摘要： Coronavirus S ectodomain trimers stabilized in a prefusion conformation, nucleic acid molecules and vectors encoding these proteins, and methods of their use and production are disclosed. In several embodiments, the coronavirus S ectodomain trimers and/or nucleic acid molecules can be used to generate an immune response to coronavirus in a subject. In additional embodiments, the therapeutically effective amount of the coronavirus S ectodomain trimers and/or nucleic acid molecules can be administered to a subject in a method of treating or preventing coronavirus infection.

公开(公告)号：US11248039B2

公开(公告)日：2022-02-15

申请号：US16566542

申请日：2019-09-10

申请人： VIB VZW ,  UNIVERSITEIT GENT ,  THE UNITED STATES OF AMERICA, AS REPRESENTED BY THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES ,  TRUSTEES OF DARTMOUTH COLLEGE

发明人： Xavier Saelens ,  Bert Schepens ,  Iebe Rossey ,  Barney Graham ,  Jason McLellan ,  Morgan Gilman

IPC分类号： C07K16/10 ,  A61P31/14 ,  A61K39/12 ,  A61K39/00

摘要： The present invention relates to immunoglobulin single variable domains (ISVDs) that are directed against respiratory syncytial virus (RSV). More specifically, it relates to ISVDs that bind to the prefusion form of the fusion (F) protein of RSV. The invention relates further to the use of these ISVDs for prevention and/or treatment of RSV infections, and to pharmaceutical compositions comprising these ISVDs.

公开(公告)号：US11964010B2

公开(公告)日：2024-04-23

申请号：US17194834

申请日：2021-03-08

申请人： The United States of America, as represented by the Secretary, Department of Health and Human Services ,  The Scripps Research Institute ,  Trustees of Dartmouth College

发明人： Barney Graham ,  Jason Mclellan ,  Andrew Ward ,  Robert Kirchdoerfer ,  Christopher Cottrell ,  Michael Gordon Joyce ,  Masaru Kanekiyo ,  Nianshuang Wang ,  Jesper Pallesen ,  Hadi Yassine ,  Hannah Turner ,  Kizzmekia Corbett

IPC分类号： A61K39/215 ,  A61P31/14 ,  C07K14/005 ,  C12N7/00

CPC分类号： A61K39/215 ,  A61P31/14 ,  C07K14/005 ,  C12N7/00 ,  C12N2770/20022 ,  C12N2770/20034 ,  C12N2770/20071

摘要： Coronavirus S ectodomain trimers stabilized in a prefusion conformation, nucleic acid molecules and vectors encoding these proteins, and methods of their use and production are disclosed. In several embodiments, the coronavirus S ectodomain trimers and/or nucleic acid molecules can be used to generate an immune response to coronavirus in a subject. In additional embodiments, the therapeutically effective amount of the coronavirus S ectodomain trimers and/or nucleic acid molecules can be administered to a subject in a method of treating or preventing coronavirus infection.

公开(公告)号：US12018051B2

公开(公告)日：2024-06-25

申请号：US18162530

申请日：2023-01-31

申请人： The United States of America, as represented by the Secretary, Department of Health and Human Services

发明人： Peter Collins ,  Bo Liang ,  Shirin Munir ,  Anne Schaap Nutt ,  Ursula Buchholz ,  Natalie Mackow ,  Peter Kwong ,  Barney Graham ,  Jason McLellan

IPC分类号： C07K14/005 ,  A61K39/12 ,  A61K39/155 ,  A61P31/14 ,  A61K39/00

CPC分类号： C07K14/005 ,  A61K39/12 ,  A61K39/155 ,  A61P31/14 ,  A61K2039/5254 ,  A61K2039/5256 ,  A61K2039/575 ,  A61K2039/70 ,  C07K2319/03 ,  C07K2319/033 ,  C12N2760/00022 ,  C12N2760/18534 ,  C12N2760/18571 ,  C12N2760/18622 ,  C12N2760/18634 ,  C12N2760/18641 ,  C12N2760/18643 ,  C12N2760/18671 ,  C12N2760/18722 ,  C12N2760/18734 ,  C12N2760/18741 ,  C12N2760/18743 ,  C12N2760/18771

摘要： Recombinant paramyxoviruses including a viral genome encoding a heterologous gene are provided. In several embodiments, the recombinant paramyxovirus is a recombinant parainfluenza virus, such as a recombinant PIV3 including a viral genome encoding a heterologous respiratory syncytial virus F ectodomain linked to the transmembrane domain and the cytoplasmic tail of the F protein from the PIV3. Nucleic acid molecules including the genome of a recombinant paramyxoviruses are also provided. The recombinant viruses may advantageously be used in vaccine formulations, such as for vaccines against parainfluenza virus and respiratory syncytial virus.

公开(公告)号：US10858400B2

公开(公告)日：2020-12-08

申请号：US15633578

申请日：2017-06-26

申请人： The United States of America, as represented by the Secretary, Department of Health and Human Services

发明人： Peter Kwong ,  Barney Graham ,  Jason McLellan ,  Man Chen ,  Baoshan Zhang ,  Tongqing Zhou ,  Michael Gordon Joyce ,  Yongping Yang

IPC分类号： C07K14/005

摘要： Disclosed are immunogens including a recombinant RSV F protein stabilized in a prefusion conformation. Also disclosed are nucleic acids encoding the immunogens and methods of producing the immunogens. Methods for generating an immune response in a subject are also disclosed. In some embodiments, the method is a method for treating or preventing a RSV infection in a subject by administering a therapeutically effective amount of the immunogen to the subject.

公开(公告)号：US10273288B2

公开(公告)日：2019-04-30

申请号：US16190676

申请日：2018-11-14

申请人： The United States of America, as represented by the Secretary, Department of Health and Human Services ,  Institute for Research in Biomedicine ,  The United States of America, as represented by the Secretary of the Army ,  Humabs BioMed SA

发明人： Nancy Sullivan ,  Sabue Mulangu ,  Davide Corti ,  Antonio Lanzavecchia ,  Barney Graham ,  Jean-Jacques Muyembe-Tamfun ,  John Trefry ,  Julie Ledgerwood ,  Daphne Stanley

IPC分类号： C07K16/10 ,  A61K39/00 ,  A61K39/395 ,  A61K39/42 ,  A61K45/06 ,  G01N33/569

摘要： Neutralizing antibodies and antigen binding fragments that specifically bind to Ebola virus glycoprotein are disclosed. Nucleic acids encoding these antibodies, vectors and host cells are also provided. Methods for detecting Ebola virus using the antibodies and antigen binding fragments are disclosed. The antibodies, antigen binding fragments, nucleic acids, and vectors, can be used, for example, to prevent and/or treat Ebola virus infection in a subject.

公开(公告)号：US11939356B2

公开(公告)日：2024-03-26

申请号：US17202231

申请日：2021-03-15

申请人： The United States of America, as represented by the Secretary, Department of Health and Human Services

发明人： Peter Kwong ,  Ivelin Georgiev ,  Michael Gordon Joyce ,  Masaru Kanekiyo ,  Aliaksandr Druz ,  Ulrich Baxa ,  Joseph Van Galen ,  Cheng Cheng ,  John Mascola ,  Yaroslav Tsybovsky ,  Yongping Yang ,  Barney Graham ,  Syed Mohammad Moin ,  Jeffrey Boyington

IPC分类号： C07K14/11 ,  A61K39/21 ,  C07K14/08 ,  C07K14/16 ,  C07K14/165 ,  C07K14/435 ,  C12N7/00 ,  A61K39/00

CPC分类号： C07K14/11 ,  A61K39/21 ,  C07K14/082 ,  C07K14/162 ,  C07K14/165 ,  C07K14/43563 ,  C12N7/00 ,  A61K2039/55555 ,  C07K2319/735 ,  C12N2740/16134 ,  C12N2760/16134 ,  C12N2770/20034

摘要： Disclosed are recombinant insect ferritin nanoparticles that can be used to display two different trimeric antigens at an equal ratio. Also disclosed are nucleic acids encoding the recombinant insect ferritin nanoparticles and methods of producing the recombinant insect ferritin nanoparticles. Methods for eliciting an immune response in a subject are also provided.

公开(公告)号：US11174292B2

公开(公告)日：2021-11-16

申请号：US16089993

申请日：2017-03-29

申请人： The United States of America, as represented by the Secretary, Department of Health and Human Services

发明人： Peter Kwong ,  Barney Graham ,  John Mascola ,  Li Ou ,  Aliaksandr Druz ,  Man Chen ,  Wing-Pui Kong ,  Ivelin Stefanov Georgiev ,  Emily Rundlet ,  Michael Gordon Joyce ,  Yaroslav Tsybovsky ,  Paul Thomas ,  Marie Pancera ,  Mallika Sastry ,  Cinque Soto ,  Joseph Van Galen ,  Guillaume Stewart-Jones ,  Yongping Yang ,  Baoshan Zhang ,  Ulrich Baxa

IPC分类号： C07K14/005 ,  A61K39/12 ,  A61P37/04 ,  A61P31/14 ,  A61K39/00

摘要： Embodiments of a recombinant Respiratory Syncytial Virus (RSV) F ectodomain trimer stabilized in a prefusion conformation are provided. Also disclosed are nucleic acids encoding the RSV F ectodomain trimer and methods of producing the RSV F ectodomain trimer. Methods for inducing an immune response in a subject are also disclosed. In some embodiments, the method can be a method for treating or preventing a RSV infection in a subject by administering a therapeutically effective amount of the recombinant RSV F ectodomain trimer to the subject.

公开(公告)号：US10160795B2

公开(公告)日：2018-12-25

申请号：US15526661

申请日：2015-11-13

申请人： The United States of America, as represented by the Secretary, Department of Health and Human Services ,  Institute for Research in Biomedicine ,  The United States of America as represented by the Secretary of the Army ,  Humabs BioMed SA

发明人： Nancy Sullivan ,  Sabue Mulangu ,  Davide Corti ,  Antonio Lanzavecchia ,  Barney Graham ,  Jean-Jacques Muyembe-Tamfun ,  John Trefry ,  Julie Ledgerwood ,  Daphne Stanley

IPC分类号： C07K16/10 ,  A61K39/00 ,  A61K39/395 ,  A61K45/06 ,  C07K16/00 ,  G01N33/569

摘要： Neutralizing antibodies and antigen binding fragments that specifically bind to Ebola virus glycoprotein are disclosed. Nucleic acids encoding these antibodies, vectors and host cells are also provided. Methods for detecting Ebola virus using the antibodies and antigen binding fragments are disclosed. The antibodies, antigen binding fragments, nucleic acids, and vectors, can be used, for example, to prevent and/or treat Ebola virus infection in a subject.