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    In vitro amplification/expansion of CD34.sup.+ stem and progenitor cells
    1.
    发明授权
    In vitro amplification/expansion of CD34.sup.+ stem and progenitor cells 失效
    CD34 +干细胞和祖细胞的体外扩增/扩增

    公开(公告)号：US5599703A

    公开(公告)日：1997-02-04

    申请号：US142569

    申请日：1993-10-28

    申请人： Thomas A. Davis ,  Steven Kessler ,  Douglas H. Robinson

    发明人： Thomas A. Davis ,  Steven Kessler ,  Douglas H. Robinson

    IPC分类号： A61K35/12 ,  C12N5/0789 ,  C12N5/00 ,  A01N63/00 ,  A01N65/00

    CPC分类号： C12N5/0647 ,  A61K2035/124 ,  C12N2501/125 ,  C12N2501/22 ,  C12N2501/23 ,  C12N2502/28

    摘要： The present invention relates to a method of amplifying in vitro stemcells. In this method hematopoietic CD34.sup.+ stem and progenitor cells are isolated from human bone marrow and contacted with endothelial cells. The contacted stem cells and endothelial cells are cultured in the presence of at least one cytokine in an amount sufficient to support amplification/expansion of the hematopoietic CD34.sup.+ stem and progenitor cells. This method produces increased yields of hematopoietic CD34.sup.+ stem and progenitor cells which can be used in human therapeutics.

    摘要翻译： 本发明涉及一种放大体外干细胞的方法。 在这种方法中，造血CD34 +干细胞和祖细胞从人骨髓中分离并与内皮细胞接触。 在至少一种细胞因子存在下培养接触的干细胞和内皮细胞，其量足以支持造血CD34 +干细胞和祖细胞的扩增/扩增。 该方法产生可用于人类治疗剂的造血CD34 +干细胞和祖细胞的增加的产量。

    METHODS TO ACCELERATE THE ISOLATION OF NOVEL CELL STRAINS FROM PLURIPOTENT STEM CELLS AND CELLS OBTAINED THEREBY
    2.
    发明申请
    METHODS TO ACCELERATE THE ISOLATION OF NOVEL CELL STRAINS FROM PLURIPOTENT STEM CELLS AND CELLS OBTAINED THEREBY 审中-公开
    用于加速从其中获得的细胞干细胞和细胞分离新细胞株的方法

    公开(公告)号：US20100184033A1

    公开(公告)日：2010-07-22

    申请号：US12504630

    申请日：2009-07-16

    申请人： Michael D. West ,  Geoffrey Sargent ,  James T. Murai ,  Steven Kessler ,  Karen Chapman ,  David Larocca

    发明人： Michael D. West ,  Geoffrey Sargent ,  James T. Murai ,  Steven Kessler ,  Karen Chapman ,  David Larocca

    IPC分类号： C12Q1/68 ,  C12N5/07 ,  C12N5/071

    CPC分类号： C12N5/0606

    摘要： Aspects of the present invention relate to methods to differentiate pluripotent primordial stem cells, such as human embryonic stem (“hES”) cells, human embryonic germ (“hEG”) cells, human embryo-derived (“hED”) cells and human embryonal carcinoma (“hEC”) cells, to obtain subpopulations of cells from heterogeneous mixtures of cells, wherein the subpopulation of cells possess reduced differentiation potential compared to the original pluripotent stem cells and where the subpopulation is capable of being propagated 20 or more population doublings. This invention also provides novel compositions of such subpopulations of cells and methods to propagate and differentiate said cells.

    摘要翻译： 本发明的方面涉及区分多能原始干细胞（例如人胚胎干细胞（“hES”）细胞，人胚胎细胞（“hEG”）细胞，人胚来源的（“hED”）细胞和人胚胎干细胞 癌细胞（“hEC”），以从细胞的异质混合物中获得细胞亚群，其中与原始多能干细胞相比，细胞亚群具有降低的分化潜力，并且亚群能够传播20种或更多种群倍增。 本发明还提供了这种细胞亚群的新组合物和传播和分化所述细胞的方法。

    Methods and Compositions for Producing Endothelial Progenitor Cells from Pluripotent Stem Cells
    4.
    发明申请
    Methods and Compositions for Producing Endothelial Progenitor Cells from Pluripotent Stem Cells 审中-公开
    用于从多能干细胞产生内皮祖细胞的方法和组合物

    公开(公告)号：US20120295347A1

    公开(公告)日：2012-11-22

    申请号：US13477002

    申请日：2012-05-21

    申请人： Steven Kessler

    发明人： Steven Kessler

    IPC分类号： C12N5/071 ,  C12N5/0735

    CPC分类号： C12N5/0692 ,  C12N2501/115 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/165 ,  C12N2501/727 ,  C12N2506/02 ,  C12N2533/52

    摘要： Aspects of the present invention are drawn to methods and compositions for producing endothelial progenitor cells (EPCs) in vitro from pluripotent stem cells and compositions containing such EPCs. The methods produce sufficient EPCs to use in therapeutic applications. In certain embodiments the EPCs are bipotent, giving rise to both vascular and lymphatic endothelial cells. In certain embodiments, EPCs express one or more of the following gene products: LYVE-1, PV-1/PAL-E, CD31, and CD34.

    摘要翻译： 本发明的方面涉及从多能干细胞和含有这些EPC的组合物在体外产生内皮祖细胞（EPCs）的方法和组合物。 该方法产生足够的EPCs用于治疗应用。 在某些实施方案中，EPCs是双向的，产生血管和淋巴内皮细胞。 在某些实施方案中，EPCs表达以下基因产物中的一种或多种：LYVE-1，PV-1 / PAL-E，CD31和CD34。

    Methods for screening antibody-producing cells on heterogeneous antigen substrates
    5.
    发明申请
    Methods for screening antibody-producing cells on heterogeneous antigen substrates 审中-公开
    在异种抗原底物上筛选抗体产生细胞的方法

    公开(公告)号：US20060073095A1

    公开(公告)日：2006-04-06

    申请号：US10224997

    申请日：2002-08-21

    申请人： Steven Kessler

    发明人： Steven Kessler

    IPC分类号： A61K51/00 ,  C12P21/04 ,  C12N5/06

    CPC分类号： G01N33/56972 ,  C07K16/00 ,  C07K16/005

    摘要： Methods and compounds are disclosed that relate to screening and selection of monoclonal antibodies specific for antigens in heterogeneous antigen mixtures. Antibody-secreting cells such as hybridomas are modified to make them capable of directly binding antigens by capturing their secreted antibody products onto their surface membranes in appropriate binding density and orientation. Selectivity of binding to novel or desired antigens is achieved by first reacting the antigen mixtures affixed to a solid substrate with a polyclonal antibody library that prevents access to the majority of antigens or epitopes other than those that are novel or desired.

    摘要翻译： 公开了涉及在异种抗原混合物中抗原特异性的单克隆抗体的筛选和选择的方法和化合物。 修饰抗体分泌细胞如杂交瘤以使其能够通过以适当的结合密度和取向将其分泌的抗体产物捕获到其表面膜上而直接结合抗原。 通过首先将固定在固体底物上的抗原混合物与多克隆抗体文库反应，来实现与新型或所需抗原结合的选择性，所述多克隆抗体文库阻止接近除了新颖或期望的那些以外的大多数抗原或表位。