公开(公告)号：US20100184033A1

公开(公告)日：2010-07-22

申请号：US12504630

申请日：2009-07-16

Applicant: Michael D. West ,  Geoffrey Sargent ,  James T. Murai ,  Steven Kessler ,  Karen Chapman ,  David Larocca

Inventor： Michael D. West ,  Geoffrey Sargent ,  James T. Murai ,  Steven Kessler ,  Karen Chapman ,  David Larocca

IPC: C12Q1/68 ,  C12N5/07 ,  C12N5/071

CPC classification number: C12N5/0606

Abstract: Aspects of the present invention relate to methods to differentiate pluripotent primordial stem cells, such as human embryonic stem (“hES”) cells, human embryonic germ (“hEG”) cells, human embryo-derived (“hED”) cells and human embryonal carcinoma (“hEC”) cells, to obtain subpopulations of cells from heterogeneous mixtures of cells, wherein the subpopulation of cells possess reduced differentiation potential compared to the original pluripotent stem cells and where the subpopulation is capable of being propagated 20 or more population doublings. This invention also provides novel compositions of such subpopulations of cells and methods to propagate and differentiate said cells.

Abstract translation: 本发明的方面涉及区分多能原始干细胞（例如人胚胎干细胞（“hES”）细胞，人胚胎细胞（“hEG”）细胞，人胚来源的（“hED”）细胞和人胚胎干细胞 癌细胞（“hEC”），以从细胞的异质混合物中获得细胞亚群，其中与原始多能干细胞相比，细胞亚群具有降低的分化潜力，并且亚群能够传播20种或更多种群倍增。 本发明还提供了这种细胞亚群的新组合物和传播和分化所述细胞的方法。

公开(公告)号：US20120001475A1

公开(公告)日：2012-01-05

申请号：US12828194

申请日：2010-06-30

Applicant: Gregory Henry Dubay ,  Kevin W. Ingall ,  Randall Steven Kessler

Inventor： Gregory Henry Dubay ,  Kevin W. Ingall ,  Randall Steven Kessler

IPC: E21C35/19 ,  E21C35/18 ,  B23P6/00 ,  E21C25/10

CPC classification number: E21C35/18 ,  E21C25/10 ,  E21C35/19 ,  E21C2035/1826

Abstract: A base block for a cutting drum is disclosed. The base block may have a first end. The first end may have an opening configured to receive a tool. The first end may also have a flat surface surrounding the opening. The base block may have a second end configured to abut the cutting drum. The base block may further have an internal passageway commencing at the opening configured to receive the tool. The passageway may define a longitudinal axis that is offset by an acute angle from being perpendicular to the flat surface.

Abstract translation: 公开了一种用于切割滚筒的基座。 基座可以具有第一端。 第一端可以具有构造成接收工具的开口。 第一端还可以具有围绕开口的平坦表面。 基座可以具有构造成邻接切割滚筒的第二端。 基座还可以具有从配置成接收工具的开口开始的内部通道。 通道可以限定纵向轴线，该纵向轴线与垂直于平坦表面的锐角相抵消。

公开(公告)号：US20070244497A1

公开(公告)日：2007-10-18

申请号：US11735798

申请日：2007-04-16

Applicant: Russell Ahlberg ,  Charles Hart ,  Steven Kessler

Inventor： Russell Ahlberg ,  Charles Hart ,  Steven Kessler

IPC: A61B17/3201

CPC classification number: A61B17/3201 ,  A61B17/320016 ,  B24B3/52 ,  B26B13/06 ,  B26B13/08

Abstract: A laparoscopic scissor with a shaft for use through an access port, such as a trocar cannula, with one or more parabolic, cubic, quadratic or higher order cutting blades extending from the shaft is provided.

Abstract translation: 提供了一种具有轴的腹腔镜剪刀，其通过诸如套管针套管的进入口使用，具有从轴延伸的一个或多个抛物线，立方，二次或更高次级的切割刀片。

公开(公告)号：US08602503B2

公开(公告)日：2013-12-10

申请号：US12828194

申请日：2010-06-30

Applicant: Gregory Henry Dubay ,  Kevin W. Ingall ,  Randall Steven Kessler

Inventor： Gregory Henry Dubay ,  Kevin W. Ingall ,  Randall Steven Kessler

IPC: E21C35/18 ,  E21C35/19

CPC classification number: E21C35/18 ,  E21C25/10 ,  E21C35/19 ,  E21C2035/1826

Abstract: A base block for a cutting drum is disclosed. The base block may have a first end. The first end may have an opening configured to receive a tool. The first end may also have a flat surface surrounding the opening. The base block may have a second end configured to abut the cutting drum. The base block may further have an internal passageway commencing at the opening configured to receive the tool. The passageway may define a longitudinal axis that is offset by an acute angle from being perpendicular to the flat surface.

Abstract translation: 公开了一种用于切割滚筒的基座。 基座可以具有第一端。 第一端可以具有构造成接收工具的开口。 第一端还可以具有围绕开口的平坦表面。 基座可以具有构造成邻接切割滚筒的第二端。 基座还可以具有从配置成接收工具的开口开始的内部通道。 通道可以限定纵向轴线，该纵向轴线与垂直于平坦表面的锐角相抵消。

公开(公告)号：US20080070303A1

公开(公告)日：2008-03-20

申请号：US11604047

申请日：2006-11-21

Applicant: Michael West ,  Geoffrey Sargent ,  James Murai ,  Steven Kessler ,  Karen Chapman

Inventor： Michael West ,  Geoffrey Sargent ,  James Murai ,  Steven Kessler ,  Karen Chapman

IPC: C12N5/02 ,  C12N5/00

CPC classification number: C12N5/0662 ,  A61K35/30 ,  A61K35/34 ,  A61K35/39 ,  A61K35/407 ,  C12N5/0606 ,  C12N5/0619 ,  C12N5/0652 ,  C12N5/0657 ,  C12N5/067 ,  C12N5/0676 ,  C12N2506/02

Abstract: This invention generally relates to methods to differentiate pluripotent stem cells, such as embryonic stem, embryonic germ, or embryo-derived cells, to obtain subpopulations of cells from heterogeneous mixtures of cells wherein the subpopulation of cells possess reduced differentiation potential compared to the original pluripotent stem cells and where the subpopulation is capable of being propagated.

Abstract translation: 本发明一般涉及区分多能干细胞（例如胚胎干，胚胎胚芽或胚源性细胞）的方法，以从细胞的不均匀混合物获得细胞亚群，其中与原始多能性相比，细胞亚群具有降低的分化潜能 干细胞和亚群能够传播的地方。

公开(公告)号：US5599703A

公开(公告)日：1997-02-04

申请号：US142569

申请日：1993-10-28

Applicant: Thomas A. Davis ,  Steven Kessler ,  Douglas H. Robinson

Inventor： Thomas A. Davis ,  Steven Kessler ,  Douglas H. Robinson

IPC: A61K35/12 ,  C12N5/0789 ,  C12N5/00 ,  A01N63/00 ,  A01N65/00

CPC classification number: C12N5/0647 ,  A61K2035/124 ,  C12N2501/125 ,  C12N2501/22 ,  C12N2501/23 ,  C12N2502/28

Abstract: The present invention relates to a method of amplifying in vitro stemcells. In this method hematopoietic CD34.sup.+ stem and progenitor cells are isolated from human bone marrow and contacted with endothelial cells. The contacted stem cells and endothelial cells are cultured in the presence of at least one cytokine in an amount sufficient to support amplification/expansion of the hematopoietic CD34.sup.+ stem and progenitor cells. This method produces increased yields of hematopoietic CD34.sup.+ stem and progenitor cells which can be used in human therapeutics.

Abstract translation: 本发明涉及一种放大体外干细胞的方法。 在这种方法中，造血CD34 +干细胞和祖细胞从人骨髓中分离并与内皮细胞接触。 在至少一种细胞因子存在下培养接触的干细胞和内皮细胞，其量足以支持造血CD34 +干细胞和祖细胞的扩增/扩增。 该方法产生可用于人类治疗剂的造血CD34 +干细胞和祖细胞的增加的产量。

公开(公告)号：US20120295347A1

公开(公告)日：2012-11-22

申请号：US13477002

申请日：2012-05-21

Applicant: Steven Kessler

Inventor： Steven Kessler

IPC: C12N5/071 ,  C12N5/0735

CPC classification number: C12N5/0692 ,  C12N2501/115 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/165 ,  C12N2501/727 ,  C12N2506/02 ,  C12N2533/52

Abstract: Aspects of the present invention are drawn to methods and compositions for producing endothelial progenitor cells (EPCs) in vitro from pluripotent stem cells and compositions containing such EPCs. The methods produce sufficient EPCs to use in therapeutic applications. In certain embodiments the EPCs are bipotent, giving rise to both vascular and lymphatic endothelial cells. In certain embodiments, EPCs express one or more of the following gene products: LYVE-1, PV-1/PAL-E, CD31, and CD34.

Abstract translation: 本发明的方面涉及从多能干细胞和含有这些EPC的组合物在体外产生内皮祖细胞（EPCs）的方法和组合物。 该方法产生足够的EPCs用于治疗应用。 在某些实施方案中，EPCs是双向的，产生血管和淋巴内皮细胞。 在某些实施方案中，EPCs表达以下基因产物中的一种或多种：LYVE-1，PV-1 / PAL-E，CD31和CD34。

公开(公告)号：US20060073095A1

公开(公告)日：2006-04-06

申请号：US10224997

申请日：2002-08-21

Applicant: Steven Kessler

Inventor： Steven Kessler

IPC: A61K51/00 ,  C12P21/04 ,  C12N5/06

CPC classification number: G01N33/56972 ,  C07K16/00 ,  C07K16/005

Abstract: Methods and compounds are disclosed that relate to screening and selection of monoclonal antibodies specific for antigens in heterogeneous antigen mixtures. Antibody-secreting cells such as hybridomas are modified to make them capable of directly binding antigens by capturing their secreted antibody products onto their surface membranes in appropriate binding density and orientation. Selectivity of binding to novel or desired antigens is achieved by first reacting the antigen mixtures affixed to a solid substrate with a polyclonal antibody library that prevents access to the majority of antigens or epitopes other than those that are novel or desired.

Abstract translation: 公开了涉及在异种抗原混合物中抗原特异性的单克隆抗体的筛选和选择的方法和化合物。 修饰抗体分泌细胞如杂交瘤以使其能够通过以适当的结合密度和取向将其分泌的抗体产物捕获到其表面膜上而直接结合抗原。 通过首先将固定在固体底物上的抗原混合物与多克隆抗体文库反应，来实现与新型或所需抗原结合的选择性，所述多克隆抗体文库阻止接近除了新颖或期望的那些以外的大多数抗原或表位。