-
公开(公告)号：US20050103629A1
公开(公告)日：2005-05-19
申请号：US10961308
申请日：2004-10-07
申请人： Tom Diller , Tom Beardslee , Regina Rooney , Joseph Amshey
发明人： Tom Diller , Tom Beardslee , Regina Rooney , Joseph Amshey
IPC分类号： G01N27/447 , G01N33/559
CPC分类号： G01N27/44795 , G01N27/44747
摘要： Provided herein are rapidly-rehydratable prior-cast, dehydrated, electrophoresis separation media particularly useful for isoelectric focusing, including methods of making, and methods of use thereof.
摘要翻译： 本文提供了对于等电聚焦特别有用的快速可再氢化的预铸，脱水，电泳分离介质，包括制备方法及其使用方法。
-
公开(公告)号：US20050202405A1
公开(公告)日：2005-09-15
申请号：US10996737
申请日：2004-11-24
申请人： Joseph Amshey , Tom Diller , Regina Rooney
发明人： Joseph Amshey , Tom Diller , Regina Rooney
IPC分类号： C12Q1/00 , C30B20060101
摘要： The present invention provides methods, compositions, and kits for protein crystallization. The present invention involves electrophoretically focusing at least a first protein species within a matrix comprising at least 2 regions of different pH, the protein being present in amount sufficient to permit crystallization within said pH gradient.
摘要翻译： 本发明提供蛋白质结晶的方法，组合物和试剂盒。 本发明涉及将包含至少两个不同pH的区域的基质中的至少第一种蛋白物质电泳聚焦，所述蛋白质的量足以允许在所述pH梯度内结晶。
-
3.发明申请Methods and apparatus for electrophoresis of prior-cast, hydratable separation media 审中-公开预浇铸水合分离介质的电泳方法和装置公开(公告)号：US20050284764A1
公开(公告)日：2005-12-29
申请号：US11179823
申请日：2005-07-11
IPC分类号： B01D57/02 , B03C5/00 , C07K1/26 , C08F2/58 , G01N27/447 , G01N33/559 , G01N33/561 , B01D59/42
CPC分类号： G01N27/44795 , C07K1/26 , G01N27/44704 , G01N33/559 , G01N33/561
摘要： Methods and apparatus are presented that facilitate electrophoresis of prior-cast, hydratable separation media, usefully immobilized pH gradient (IPG) strips. The method exploits the swelling of prior-cast, hydratable separation media upon rehydration to help lodge the media in an enclosing member that permits spaced electrical communication with the enclosed separation media. The electrical communication permits a voltage gradient to be established in the enclosed separation medium sufficient to effect separation of analytes therein. Cassettes, buffer cores, electrophoresis systems and kits are presented for effecting the methods of the invention.
摘要翻译： 提出了方便和装置，其促进了预先浇铸的可水合分离介质的电泳，有用的固定化pH梯度（IPG）条带。 该方法利用了预先浇铸的可水合分离介质在再水合时的溶胀，以帮助将介质置于封闭的构件中，该封闭构件允许与封闭的分离介质间隔开的电连通。 电气通信允许在封闭的分离介质中建立足以实现其中分析物分离的电压梯度。 提出了盒，缓冲核心，电泳系统和试剂盒用于实现本发明的方法。
-
公开(公告)号：US20070290173A1
公开(公告)日：2007-12-20
申请号：US11840164
申请日：2007-08-16
CPC分类号： C07K7/08 , C07K1/1075 , C07K1/1077 , C07K1/26 , C07K14/00 , G01N27/44726
摘要： The invention relates to marker molecules for identifying physical properties of molecular species separated by the use of electrophoretic systems. The invention further relates to methods for preparing and using marker molecules.
摘要翻译： 本发明涉及用于鉴定通过使用电泳系统分离的分子物质的物理性质的标记分子。 本发明还涉及制备和使用标记分子的方法。
-
公开(公告)号：US20050023139A1
公开(公告)日：2005-02-03
申请号：US10870131
申请日：2004-06-16
申请人： Regina Rooney , Bradley Scott , Thomas Jackson , Joseph Amshey
发明人： Regina Rooney , Bradley Scott , Thomas Jackson , Joseph Amshey
IPC分类号： C02F1/469 , G01N27/26 , G01N27/447
CPC分类号： G01N27/44704 , C02F1/4696 , G01N27/44795
摘要： A multielevational cover for assembling a fluid retaining chamber on a face of an electrophoresis cassette is presented. The cover comprises a cassette-containing member, at least one chamber-defining member, and sealing means, typically sealing means that are capable of reversible attachment. Also presented are methods for contacting cassette-immobilized gels to desired fluids by assembling a fluid retaining chamber upon the cassette using the covers of the present invention.
摘要翻译： 提出了一种用于在电泳盒的表面上组装流体保持室的多电极盖。 该盖包括一个含盒的构件，至少一个室限定构件和密封装置，通常是能够可逆地附接的密封装置。 还提出了通过使用本发明的盖子将盒固定的凝胶与所需流体接触的方法，该流体保持室通过在盒上组装。
-
6.发明授权公开(公告)号：US08034223B2
公开(公告)日：2011-10-11
申请号：US12485379
申请日：2009-06-16
IPC分类号： G01N27/447 , G01N27/453
CPC分类号： C07K1/26 , G01N27/44704 , G01N27/44747 , G01N27/44795 , G01N33/559 , G01N33/561
摘要： Methods and apparatus are presented that facilitate electrophoresis of prior-cast, hydratable separation media, usefully immobilized pH gradient (IPG) strips. The method exploits the swelling of prior-cast, hydratable separation media upon rehydration to help lodge the media in an enclosing member that permits spaced electrical communication with the enclosed separation media. The electrical communication permits a voltage gradient to be established in the enclosed separation medium sufficient to effect separation of analytes therein. Cassettes, buffer cores, electrophoresis systems and kits are presented for effecting the methods of the invention.
摘要翻译： 提出了方便和装置，其促进了预先浇铸的可水合分离介质的电泳，有用的固定化pH梯度（IPG）条带。 该方法利用了预先浇铸的可水合分离介质在再水合时的溶胀，以帮助将介质置于封闭的构件中，该封闭构件允许与封闭的分离介质间隔开的电连通。 电气通信允许在封闭的分离介质中建立足以实现其中分析物分离的电压梯度。 提出了盒，缓冲核心，电泳系统和试剂盒用于实现本发明的方法。
-
公开(公告)号：US08444838B2
公开(公告)日：2013-05-21
申请号：US12392851
申请日：2009-02-25
申请人： Joseph Amshey , Thomas Diller , Regina Rooney
发明人： Joseph Amshey , Thomas Diller , Regina Rooney
IPC分类号： G01N27/447 , G01N27/453
摘要： The present invention provides methods, compositions, and kits for protein crystallization. The present invention involves electrophoretically focusing at least a first protein species within a matrix comprising at least 2 regions of different pH, the protein being present in amount sufficient to permit crystallization within said pH gradient.
摘要翻译： 本发明提供蛋白质结晶的方法，组合物和试剂盒。 本发明涉及将包含至少两个不同pH的区域的基质中的至少第一种蛋白物质电泳聚焦，所述蛋白质的量足以允许在所述pH梯度内结晶。
-
公开(公告)号：US20070026479A1
公开(公告)日：2007-02-01
申请号：US11475260
申请日：2006-06-27
申请人： Thomas Beardslee , Cinnamon Bolz , Timothy Updyke , Regina Rooney
发明人： Thomas Beardslee , Cinnamon Bolz , Timothy Updyke , Regina Rooney
CPC分类号： G01N27/44704 , C07K1/16 , C07K1/26
摘要： Marker sets are provided for use on nondenaturing gels. The protein molecular weight markers are provided in liquid form, and are stable in liquid form for at least two months at 4 degrees C. and at least one year at −20 degrees C. Methods of using the markers and kits containing stable native protein markers in liquid form for determining molecular mass of proteins using electrophoresis are also provided. Furthermore, methods for generating revenue by selling the liquid molecular weight markers, are provided.
摘要翻译： 标记组提供用于非变性凝胶。 蛋白质分子量标记物以液体形式提供，并且在4℃下以液体形式稳定至少两个月，在-20℃下至少一年。使用含有稳定天然蛋白质标记物的标记物和试剂盒的方法 还提供用于使用电泳测定蛋白质分子量的液体形式。 此外，提供了通过销售液体分子量标记来产生收入的方法。
-
9.发明授权公开(公告)号：US08288155B2
公开(公告)日：2012-10-16
申请号：US11777919
申请日：2007-07-13
申请人： John R. Havens , Thomas J. Onofrey , Charles H. Greef , Gregory J. Kevorkian , Jain Krotz , Kristie L. Lykstad , Daniel E. Raymond , Howard R. Reese , Regina Rooney , John J. Scott
发明人： John R. Havens , Thomas J. Onofrey , Charles H. Greef , Gregory J. Kevorkian , Jain Krotz , Kristie L. Lykstad , Daniel E. Raymond , Howard R. Reese , Regina Rooney , John J. Scott
CPC分类号： C40B50/14 , B01J19/0046 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00626 , B01J2219/00635 , B01J2219/00637 , B01J2219/00639 , B01J2219/00641 , B01J2219/00653 , B01J2219/00659 , B01J2219/00713 , B01J2219/0072 , C07B2200/11 , C07C319/02 , C07H21/00 , C40B40/06 , G01N33/54353 , G01N33/545 , C07C323/60
摘要： Methods of addressing a biomolecule to a selectively addressable electrode are described. A permeation layer overlying a plurality of selectively addressable electrodes is provided. The permeation layer includes a reactive group that is adapted to bond to a biomolecule and that requires activation through a chemical transformation before bonding to the biomolecule. At least one selectively addressable electrode is biased such that a pH change occurs in an overlying solution of the at least one selectively addressable electrode. The reactive group in a portion of the permeation layer above the at least one selectively addressable electrode is then chemically transformed to an activated reactive group as a result of the pH change. A biomolecule is then bound to the permeation layer overlying the at least one selectively addressable electrode through the activated reactive group.
摘要翻译： 描述了将生物分子寻址到选择性寻址电极的方法。 提供了覆盖多个选择性寻址电极的渗透层。 渗透层包括适于结合生物分子的反应性基团，并且在结合到生物分子之前需要通过化学转化的活化。 至少一个选择性寻址的电极被偏压，使得在所述至少一个选择性寻址电极的上覆溶液中发生pH变化。 作为pH变化的结果，将至少一个可选寻址电极上方的渗透层的一部分中的反应基团化学转化成活化的反应性基团。 然后将生物分子与穿过活化的反应性基团的至少一个选择性寻址电极重叠的渗透层结合。
-
公开(公告)号：US20110226620A1
公开(公告)日：2011-09-22
申请号：US12786406
申请日：2010-05-24
IPC分类号： G01N27/447 , C07K7/08 , C07K14/245 , C07K1/13 , C25B7/00
CPC分类号： C07K7/08 , C07K1/1075 , C07K1/1077 , C07K1/26 , C07K14/00 , G01N27/44726
摘要： The invention relates to marker molecules for identifying physical properties of molecular species separated by the use of electrophoretic systems. The invention further relates to methods for preparing and using marker molecules.
摘要翻译： 本发明涉及用于鉴定通过使用电泳系统分离的分子物质的物理性质的标记分子。 本发明还涉及制备和使用标记分子的方法。
-
-
-
-
-
-
-
-
-
搜索结果
11 条记录 (耗时 0.026 秒)
