公开(公告)号：US07604776B2

公开(公告)日：2009-10-20

申请号：US12113643

申请日：2008-05-01

申请人： Tomohiro Yamamoto ,  Toshihiko Yoshioka ,  Nobuhiko Ozaki

发明人： Tomohiro Yamamoto ,  Toshihiko Yoshioka ,  Nobuhiko Ozaki

IPC分类号： G01N35/00 ,  G01N35/08

CPC分类号： G01N35/00069 ,  G01N35/08 ,  G01N2035/00544 ,  Y10T436/111666 ,  Y10T436/25 ,  Y10T436/25375

摘要： A reaction of a sample mixture liquid is detected accurately, by allowing a solid reagent to be dissolved and reacted in a liquid sample quickly and accurately. A disc main body, and at least one sample mixing unit provided at the disc main body are provided, and the sample mixing unit is provided with: a liquid sample reserve unit; a reagent chamber capable of disposing a plurality of the solid reagents; and a measurement chamber to which a sample mixture liquid containing a liquid sample and a solid reagent mixed in the reagent chamber is supplied. The reagent chamber has a form which allows a plurality of the solid reagents to be disposed in the direction substantially parallel to the radial direction of the disc in which centrifugal force is generated, or in the direction substantially perpendicular to the radial direction of the disc.

摘要翻译： 通过使固体试剂在液体样品中溶解和反应迅速而准确地准确地检测样品混合液体的反应。 设置盘主体和设置在盘主体上的至少一个样品混合单元，样品混合单元设置有：液体样品储备单元; 能够配置多个固体试剂的试剂室; 以及测量室，供给包含液体试样和混合在试剂室中的固体试剂的样品混合液。 试剂室具有允许多个固体试剂在基本上平行于产生离心力的盘的径向方向或者基本上垂直于盘的径向方向的方向上设置的形式。

公开(公告)号：US08168444B2

公开(公告)日：2012-05-01

申请号：US12595077

申请日：2008-04-04

申请人： Nobuhiko Ozaki ,  Tomohiro Yamamoto

发明人： Nobuhiko Ozaki ,  Tomohiro Yamamoto

IPC分类号： B01L3/00

CPC分类号： G01N35/10 ,  G01N2035/0449 ,  G01N2035/1034 ,  Y10T436/25 ,  Y10T436/2575

摘要： A substrate having a channel portion in which a liquid transfers, wherein, even if the channel portion has no micro-channel interconnecting chambers, a liquid can transfer stepwise from one region to the next region according to the speed of rotation of the substrate. The substrate can be rotated about a rotation axis as the center, and has the channel portion including the chambers formed therein. The inner wall of each chamber has a first area including an area intersecting with a centrifugal direction from the center; and also has a second area placed at a position farther from the center than the first area, and including a surface intersecting with the centrifugal direction from the center. Further, the first area has a region for holding a droplet of a liquid provided; and also has a region where a contact with the droplet expands when the substrate is rotated, and that communicate with the second area.

摘要翻译： 具有液体转移的通道部分的基板，其中即使通道部分没有微通道互连室，液体也可以根据基板的旋转速度从一个区域逐步转移到下一个区域。 基板可以围绕作为中心的旋转轴线旋转，并且具有包括形成在其中的室的通道部分。 每个室的内壁具有第一区域，其包括从中心与离心方向相交的区域; 并且还具有放置在比第一区域更远离中心的位置的第二区域，并且包括从中心与离心方向相交的表面。 此外，第一区域具有用于保持设置的液体的液滴的区域; 并且还具有当基板旋转时与液滴的接触膨胀并且与第二区域连通的区域。

公开(公告)号：US20090238724A1

公开(公告)日：2009-09-24

申请号：US12092242

申请日：2006-10-31

申请人： Tomohiro Yamamoto ,  Nobuhiko Ozaki

发明人： Tomohiro Yamamoto ,  Nobuhiko Ozaki

IPC分类号： G01N33/00 ,  B05D3/00

CPC分类号： G01N35/00069 ,  G01N35/08

摘要： It is an object of the present invention to provide a disc for analyzing a fluid sample, which can analyze the fluid sample with accuracy by having the fluid sample react quickly and uniformly with the solid reagent held in the chamber. The disc has a chamber is defined by a gap formed with at least two opening, and defined by a spacer intervening between an upper substrate and a lower substrate, the upper substrate having a convex section, the lower substrate having a concave section, the reagent being attached to the concave section of the lower substrate.

摘要翻译： 本发明的目的是提供一种用于分析流体样品的盘，其可以通过使流体样品与保持在室中的固体试剂快速且均匀地反应而精确地分析流体样品。 所述盘具有由形成有至少两个开口的间隙限定的间隔，所述间隙由介于上基板和下基板之间的隔离物限定，所述上基板具有凸部，所述下基板具有凹部，所述试剂 附着在下基板的凹部。

公开(公告)号：US08329116B2

公开(公告)日：2012-12-11

申请号：US12741435

申请日：2008-11-21

申请人： Shuhei Tanaka ,  Tomohiro Yamamoto ,  Nobuhiko Ozaki ,  Shinki Kojima

发明人： Shuhei Tanaka ,  Tomohiro Yamamoto ,  Nobuhiko Ozaki ,  Shinki Kojima

IPC分类号： G01N15/06 ,  G01N33/00 ,  G01N33/48

CPC分类号： G01N33/54366 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2300/0645 ,  B01L2300/0877 ,  B01L2300/0887 ,  B01L2400/0415 ,  Y10T436/11

摘要： Provided is a measuring chip, which can spot a specimen liquid of a minute quantity for an immune agglutination easily and precisely to the measuring chip and which can prevent the flow of the specimen liquid and the contamination of the specimen. The measuring chip comprises at least two substrates, a spacer arranged between the substrates, at least one passage made of the substrates and the spacer into a hollow shape and having two open ends, counter electrodes for applying an electric field, and a specimen-spotted portion to be spotted with the specimen. The specimen-spotted portion has a structure made of a square, circular, elliptical or sector shape by that spacer. Moreover, the specimen-spotted portion has two open ends, one of which is connected to one open end of that passage.

摘要翻译： 提供了一种测量芯片，其可以容易且准确地将测量芯片上的微量的微量样品液体点样，并且可以防止样品液体的流动和样品的污染。 测量芯片包括至少两个基板，布置在基板之间的间隔件，由基板和间隔件形成中空形状的至少一个通道，并具有两个开口端，用于施加电场的对电极和被检测点 用样品点样的部分。 样品点状部分具有由该间隔物形成的正方形，圆形，椭圆形或扇形的结构。 此外，样本点状部分具有两个开口端，其中一个连接到该通道的一个开口端。

公开(公告)号：US20100266452A1

公开(公告)日：2010-10-21

申请号：US12741435

申请日：2008-11-21

申请人： Shuhei Tanaka ,  Tomohiro Yamamoto ,  Nobuhiko Ozaki ,  Shinki Kojima

发明人： Shuhei Tanaka ,  Tomohiro Yamamoto ,  Nobuhiko Ozaki ,  Shinki Kojima

IPC分类号： G01N33/544

CPC分类号： G01N33/54366 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2300/0645 ,  B01L2300/0877 ,  B01L2300/0887 ,  B01L2400/0415 ,  Y10T436/11

摘要： Provided is a measuring chip, which can spot a specimen liquid of a minute quantity for an immune agglutination easily and precisely to the measuring chip and which can prevent the flow of the specimen liquid and the contamination of the specimen. The measuring chip comprises at least two substrates, a spacer arranged between the substrates, at least one passage made of the substrates and the spacer into a hollow shape and having two open ends, counter electrodes for applying an electric field, and a specimen-spotted portion to be spotted with the specimen. The specimen-spotted portion has a structure made of a square, circular, elliptical or sector shape by that spacer. Moreover, the specimen-spotted portion has two open ends, one of which is connected to one open end of that passage.

摘要翻译： 提供了一种测量芯片，其可以容易且准确地将测量芯片上的微量的微量样品液体点样，并且可以防止样品液体的流动和样品的污染。 测量芯片包括至少两个基板，布置在基板之间的间隔件，由基板和间隔件形成中空形状的至少一个通道，并具有两个开口端，用于施加电场的对电极和被检测点 用样品点样的部分。 样品点状部分具有由该间隔物形成的正方形，圆形，椭圆形或扇形的结构。 此外，样本点状部分具有两个开口端，其中一个连接到该通道的一个开口端。

公开(公告)号：US20100120166A1

公开(公告)日：2010-05-13

申请号：US12595077

申请日：2008-04-04

申请人： Nobuhiko Ozaki ,  Tomohiro Yamamoto

发明人： Nobuhiko Ozaki ,  Tomohiro Yamamoto

IPC分类号： G01N1/00 ,  G01N37/00 ,  G01N35/00

CPC分类号： G01N35/10 ,  G01N2035/0449 ,  G01N2035/1034 ,  Y10T436/25 ,  Y10T436/2575

摘要： A substrate having a channel portion in which a liquid transfers, wherein, even if the channel portion has no micro-channel interconnecting chambers, a liquid can transfer stepwise from one region to the next region according to the speed of rotation of the substrate. The substrate can be rotated about a rotation axis as the center, and has the channel portion including the chambers formed therein. The inner wall of each chamber has a first area including an area intersecting with a centrifugal direction from the center; and also has a second area placed at a position farther from the center than the first area, and including a surface intersecting with the centrifugal direction from the center. Further, the first area has a region for holding a droplet of a liquid provided; and also has a region where a contact with the droplet expands when the substrate is rotated, and that communicate with the second area.

摘要翻译： 具有液体转移的通道部分的基板，其中即使通道部分没有微通道互连室，液体也可以根据基板的旋转速度从一个区域逐步转移到下一个区域。 基板可以围绕作为中心的旋转轴线旋转，并且具有包括形成在其中的室的通道部分。 每个室的内壁具有第一区域，其包括从中心与离心方向相交的区域; 并且还具有放置在比第一区域更远离中心的位置的第二区域，并且包括从中心与离心方向相交的表面。 此外，第一区域具有用于保持设置的液体的液滴的区域; 并且还具有当基板旋转时与液滴的接触膨胀并且与第二区域连通的区域。

公开(公告)号：US20100062414A1

公开(公告)日：2010-03-11

申请号：US12593986

申请日：2008-04-04

申请人： Tomohiro Yamamoto ,  Tetsuo Yukimasa ,  Ryou Hamada ,  Fumihisa Kitawaki ,  Nobuhiko Ozaki

发明人： Tomohiro Yamamoto ,  Tetsuo Yukimasa ,  Ryou Hamada ,  Fumihisa Kitawaki ,  Nobuhiko Ozaki

IPC分类号： C12Q1/00 ,  G01N31/22 ,  C12M1/34 ,  G01N21/00 ,  H01R43/00

CPC分类号： G01N33/558 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/502738 ,  B01L2200/027 ,  B01L2200/0621 ,  B01L2300/043 ,  B01L2300/0645 ,  B01L2300/0825 ,  B01L2300/087 ,  B01L2400/0406 ,  B01L2400/0633 ,  B01L2400/0688 ,  Y10T29/49117

摘要： A sample liquid analytical chip that whilst being of simple structure, has a reaction region and a detection region isolated from each other and further has liquid feed control means for a sample liquid, thereby realizing speedy reliable analysis. The sample liquid analytical chip comprises a tubular sample liquid transfer channel with its both ends open, a reaction reagent layer disposed on the internal wall surface of the sample liquid transfer channel and a detection region for detecting any physical or chemical change of the sample liquid. In the sample liquid analytical chip, the sample liquid transfer channel at an area of its wall surface is provided an opening and a lid fitted to the opening. Portion of the lid forms a hinge in cooperation with the wall surface.

摘要翻译： 一种样品液体分析芯片，其结构简单，具有彼此隔离的反应区域和检测区域，并且还具有用于样品液体的液体进料控制装置，从而实现了快速可靠的分析。 样品液体分析芯片包括其两端打开的管状样品液体传输通道，设置在样品液体传送通道的内壁表面上的反应试剂层和用于检测样品液体的任何物理或化学变化的检测区域。 在样品液体分析芯片中，在其壁表面的区域处的样品液体输送通道设置有开口和装配到开口的盖子。 盖的一部分与壁表面配合形成铰链。

公开(公告)号：US08247218B2

公开(公告)日：2012-08-21

申请号：US12133452

申请日：2008-06-05

申请人： Masaya Nakatani ,  Nobuhiko Ozaki ,  Hiroaki Oka

发明人： Masaya Nakatani ,  Nobuhiko Ozaki ,  Hiroaki Oka

IPC分类号： C12M1/34

CPC分类号： G01N33/48728

摘要： A sensing element for measuring extracellular potential including a substrate, a well provided in a substrate, a guide section provided on the wall of the well, and a detective electrode formed at a lower surface of the substrate. The guide section is for guiding drug. The well is provided at the bottom with a depression, and a first throughhole penetrating through the depression and the lower surface of the substrate. The well is for mixing a subject cell, a culture solution and the drug together. The above-configured sensing element accurately measures a change generated by a subject cell.

摘要翻译： 用于测量细胞外电位的感测元件，包括衬底，设置在衬底中的阱，设置在阱的壁上的引导部分和形成在衬底的下表面处的检测电极。 指导部分是指导药物。 井在底部设置有凹陷，并且第一通孔穿过基底的凹陷和下表面。 该井用于将主体细胞，培养液和药物混合在一起。 上述构成的感测元件精确地测量由被摄体单元产生的变化。

公开(公告)号：US07736477B2

公开(公告)日：2010-06-15

申请号：US10595275

申请日：2005-07-14

申请人： Masaya Nakatani ,  Nobuhiko Ozaki ,  Hiroaki Oka

发明人： Masaya Nakatani ,  Nobuhiko Ozaki ,  Hiroaki Oka

IPC分类号： G01N33/487

CPC分类号： G01N33/48728

摘要： A probe for measuring an electric potential of a cell includes a plate having a surface having a first cavity provided therein, and a sensor element provided in the first cavity. A second cavity is provided in the bottomsurface of the first cavity. The first flow passage having first and second openings is provided in the plate. The first and second openings of the first flow passage open to the second cavity and outside the plate, respectively. The sensor element includes a thin plate, and a supporting substrate provided around the thin plate and in the first cavity of the plate. The thin plate has a through-hole therein having a first opening and a second opening communicating with the second cavity of the plate. The first flow passage allows fluid to flow therein. A sucking device is coupled with the second opening of the first flow passage as to suck the fluid flowing in the first flow passage. This probe can measure an electric potential of a cell floating in solution as it is in this environment.

摘要翻译： 用于测量电池的电位的探针包括具有设置在其中的具有第一腔的表面的板和设置在第一腔中的传感器元件。 在第一腔的底面设有第二腔。 具有第一和第二开口的第一流动通道设置在板中。 第一流动通道的第一和第二开口分别向第二腔室和板外开口。 传感器元件包括薄板，以及设置在薄板周围和板的第一腔中的支撑基板。 薄板的通孔中具有第一开口和与板的第二腔连通的第二开口。 第一流道允许流体流入其中。 抽吸装置与第一流动通道的第二开口联接以吸收在第一流动通道中流动的流体。 该探头可以在这种环境中测量浮动在溶液中的电池的电位。

公开(公告)号：US20100132820A1

公开(公告)日：2010-06-03

申请号：US12598146

申请日：2008-04-23

申请人： Nobuhiko Ozaki ,  Airi Takagi

发明人： Nobuhiko Ozaki ,  Airi Takagi

IPC分类号： B65D25/00

CPC分类号： G01N35/00069 ,  Y10T137/86187 ,  Y10T436/11 ,  Y10T436/111666 ,  Y10T436/2575

摘要： A substrate including a channel part having a chamber in which a liquid can be fed stepwise from a chamber to another chamber at the channel part formed in the substrate, depending on the rotational speed of the substrate. A first chamber, a second chamber, a third chamber, and a channel interconnecting them are formed at the channel part formed in the substrate. Furthermore, the width and/or the depth of the first chamber is set smaller than the width and/or the depth of the second chamber. Consequently, the volume of solution subjected to centrifugal force in the first chamber is larger than the volume of solution subjected to centrifugal force in the second chamber.

摘要翻译： 基板包括具有腔室的通道部分，其中液体可以根据基板的旋转速度从腔室逐步进入形成在基板中的通道部分处的另一腔室。 第一室，第二室，第三室和与其相互连接的通道形成在形成在基板中的沟道部分。 此外，第一室的宽度和/或深度被设定为小于第二室的宽度和/或深度。 因此，在第一室中经受离心力的溶液的体积大于在第二室中经受离心力的溶液的体积。