公开(公告)号：US20090090628A1

公开(公告)日：2009-04-09

申请号：US12188792

申请日：2008-08-08

申请人： Toshiyuki Kato ,  Hitoshi Nagahora ,  Masaaki Onda ,  Shingo Tanaka

发明人： Toshiyuki Kato ,  Hitoshi Nagahora ,  Masaaki Onda ,  Shingo Tanaka

IPC分类号： G01N27/447 ,  C07H21/00 ,  C07H1/00 ,  C07D239/54

CPC分类号： C07D239/54 ,  C07D221/14 ,  C07D221/22 ,  C09B62/503 ,  C09B62/505 ,  C09B62/51 ,  C09B62/513 ,  C09B69/101 ,  C09B69/105 ,  C09B69/106 ,  C09B69/109 ,  G01N33/582 ,  G01N33/583

摘要： The objective of the invention is to provide nucleic acid derivatives with designated compounds introduced specifically into the base of nucleic acids. A DNA or RNA, or a nucleic acid molecular weight marker, wherein a designated compound (R), which is selected from any one of dyes, fluorescent dyes, RI labeled substances, and compounds capable of linking specifically to other compounds, is introduced at the N−3 position of a thymine base or uracil base in which (R′) at Position 1 is other than hydrogen atom.

摘要翻译： 本发明的目的是提供具有特异引入核酸碱基的指定化合物的核酸衍生物。 DNA或RNA或核酸分子量标记，其中从染料，荧光染料，RI标记的物质和能够与其它化合物特异性连接的化合物中选出的指定化合物（R）引入 胸腺嘧啶碱或尿嘧啶碱的N-3位，其中位置1处的（R'）不是氢原子。

公开(公告)号：US5094947A

公开(公告)日：1992-03-10

申请号：US758481

申请日：1991-09-09

申请人： Hiroshi Nakajima ,  Masaaki Onda ,  Ryoichi Turutani ,  Hayato Ishihara

发明人： Hiroshi Nakajima ,  Masaaki Onda ,  Ryoichi Turutani ,  Hayato Ishihara

IPC分类号： C12P19/02 ,  C12R1/01 ,  C12R1/07 ,  C12R1/19

CPC分类号： C12P19/02

摘要： A process for producing fructose-1,6-diphosphate comprising the steps of: (a) enzymatically converting adenosine 5'-diphosphate to adenosine 5'-triphosphate using an acetate kinase-containing microorganism or an extract of the microorganism and phosphate donor; and (b) enzymatically converting a substrate capable of being converted to glucose or fructose to fructose-1,6-diphosphate using the adenosine 5'-triphosphate resulting from step (a) and the acetate kinase-containing microorganism or the extract of the microorganism.

公开(公告)号：US5434255A

公开(公告)日：1995-07-18

申请号：US37395

申请日：1993-03-26

申请人： Tatsuo Katayama ,  Hayato Ishihara ,  Kenji Okada ,  Masaaki Onda ,  Hiroshi Nakajima

发明人： Tatsuo Katayama ,  Hayato Ishihara ,  Kenji Okada ,  Masaaki Onda ,  Hiroshi Nakajima

IPC分类号： B01D15/08 ,  B01D61/02 ,  B01D61/44 ,  B01J41/20 ,  C07H1/06 ,  C07H11/04 ,  C07H13/00

CPC分类号： C07H11/04

摘要： A process for the purification of fructose 1,6-diphosphate which comprises subjecting a fructose 1,6-diphosphate-containing solution to anion exchange column chromatography to separate fructose 1,6-diphosphate therefrom, and subsequently subjecting the resulting FDP fraction to a desalting treatment, so as to provide a highly purified FDP preparation useful as a pharmaceutical drug and the like in a high yield.

摘要翻译： 1,6-二磷酸果糖的纯化方法，该方法包括将含有1,6-二磷酸的果糖溶液进行阴离子交换柱层析，从其中分离出果糖1,6-二磷酸酯，然后将得到的FDP馏分进行脱盐 进行处理，从而以高产率提供可用作药物等的高度纯化的FDP制剂。

公开(公告)号：US5376535A

公开(公告)日：1994-12-27

申请号：US974216

申请日：1992-11-10

申请人： Masaaki Onda ,  Hiroshi Ibuki ,  Hiroshi Nakajima

发明人： Masaaki Onda ,  Hiroshi Ibuki ,  Hiroshi Nakajima

IPC分类号： C12P17/18 ,  C12N9/12 ,  C12P19/32 ,  C12R1/01 ,  C12R1/07 ,  C12P19/30

CPC分类号： C12N9/12 ,  C12P19/32

摘要： A method for producing 3'-phosphoadenosine 5'-phosphosulfate, which comprises (1) reacting adenosine 5'-triphosphate with a sulfate donor in the presence of heat-stable adenosine 5'-triphosphate sulfurylase to produce adenosine 5'-phosphosulfate, and (2) reacting said adenosine 5'-phosphosulfate with adenosine 5'-triphosphate in the presence of heat-stable adenosine 5'-phosphosulfate kinase; and another method for producing 3'-phosphoadenosine 5'-phosphosulfate which comprises (1) reacting adenosine 5'-triphosphate with a sulfate donor in the presence of adenosine 5'-triphosphate sulfurylase to produce adenosine 5'-phosphosulfate, (2) reacting said adenosine 5'-phosphosulfate with adenosine 5'-triphosphate in the presence of adenosine 5'-phosphosulfate kinase to produce 3'-phosphoadenosine 5'-phosphosulfate and adenosine 5'-diphosphate, and (3) converting said adenosine 5'-diphosphate into adenosine 5'-triphosphate in the presence of a phosphate donor and an enzyme capable of converting adenosine 5'-diphosphate into adenosine 5'-triphosphate are disclosed.

摘要翻译： 一种产生3'-磷酸腺苷5'-磷酸硫酸的方法，其包括（1）在热稳定的腺苷5'-三磷酸硫酸化酶存在下使腺苷5'-三磷酸与硫酸盐供体反应以产生腺苷5'-磷酸硫酸，以及 （2）在热稳定的腺苷5'-磷酸硫酸激酶的存在下使所述腺苷5'-磷酸硫酸与腺苷5'-三磷酸反应; 另外一种产生3'-磷酸腺苷5'-磷酸硫酸的方法，它包括（1）在5'-三磷酸磷酸腺苷酸腺苷存在下使腺苷5'-三磷酸与硫酸盐供体反应，产生腺苷5'-磷酸硫酸，（2） 在腺苷5'-磷酸硫酸激酶的存在下，腺苷5'-磷酸硫酸腺苷与腺苷5'-磷酸三磷酸腺苷产生3'-磷酸腺苷5'-磷酸硫酸和腺苷5'-二磷酸，和（3）转化所述腺苷5'-二磷酸 在磷酸盐供体和能够将腺苷5'-二磷酸腺苷转化为腺苷5'-三磷酸的酶存在下，转化为腺苷5'-三磷酸。

公开(公告)号：US5322782A

公开(公告)日：1994-06-21

申请号：US963255

申请日：1992-10-19

申请人： Hiroshi Nakajima ,  Masaaki Onda ,  Ryoichi Tsurutani ,  Kenzo Motosugi

发明人： Hiroshi Nakajima ,  Masaaki Onda ,  Ryoichi Tsurutani ,  Kenzo Motosugi

IPC分类号： C12P7/56 ,  C12P7/40 ,  C12P41/00 ,  C12P7/42 ,  C12P17/02

CPC分类号： C12P41/00 ,  C12P7/40 ,  Y10S435/877

摘要： Optically active .beta.-halolactic acids can be produced by contacting an .alpha.,.beta.-dihalopropionic acid with 2-halo acid dehalogenase. When the pH of the reaction system is above 9, this process gives optically active glycidic acid. Treatment of the optically active .beta.-halolactic acid thus obtained with an alkali also gives optically active glycidic acid.

摘要翻译： 可以通过使α，β-二卤代丙酸与2-卤代脱卤素酶接触来制备光学活性的β-阿拉伯糖酸。 当反应体系的pH高于9时，该方法得到光学活性的缩水甘油酸。 用碱处理由此获得的光学活性β-卤代乳酸也可得到光学活性的缩水甘油酸。