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公开(公告)号：US20060273187A1
公开(公告)日：2006-12-07
申请号：US11419593
申请日：2006-05-22
摘要： The invention involves developing and stabilizing cultivating droplets within a matrix of a porous medium. A cultivation medium may be selected, prepared and mixed with a surfactant. Where cells are desired to be cultured in droplets, cells may be added. The mixture may be converted into cultivating droplets. The cultivating droplets may be stabilized by introducing them to a porous medium. The porous medium may contain hydrophobic particles. Stabilized, cultivating droplets having one or more cells may form an aseptic microenvironment for the concentrated growth of cells.
摘要翻译： 本发明涉及在多孔介质的基质内培养和稳定培养液滴。 培养基可以选择，制备并与表面活性剂混合。 当需要细胞培养液滴时，可加入细胞。 混合物可以转化为培养液滴。 培养液滴可以通过将它们引入多孔介质来稳定。 多孔介质可能含有疏水性颗粒。 具有一个或多个细胞的稳定化培养液滴可形成用于细胞浓缩生长的无菌微环境。
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2.发明申请Electrospray Neutralization Process and Apparatus for Generation of Nano-Aerosol and Nano-Structured Materials 有权用于生成纳米气溶胶和纳米结构材料的电喷雾中和过程和装置公开(公告)号：US20070113530A1
公开(公告)日：2007-05-24
申请号：US11561138
申请日：2006-11-17
IPC分类号： B01D46/00
CPC分类号： B01D46/0001 , B01D39/1623 , B01D46/10 , B01D2239/0435 , B01D2275/10 , D01D5/0007
摘要： The claimed invention describes methods and apparatuses for manufacturing nano-aerosols and nano-structured materials based on the neutralization of charged electrosprayed products with oppositely charged electrosprayed products. Electrosprayed products include molecular ions, nano-clusters and nano-fibers. Nano-aerosols can be generated when neutralization occurs in the gas phase. Neutralization of electrospan nano-fibers with molecular ions and charged nano-clusters may result in the formation of fibrous aerosols or free nano-mats. Nano-mats can also be produced on a suitable substrate, forming efficient nano-filters.
摘要翻译： 所要求保护的发明描述了基于带有相反电荷的电喷雾产物的带电电喷雾产物的中和来制造纳米气溶胶和纳米结构材料的方法和装置。 电喷产品包括分子离子，纳米簇和纳米纤维。 当在气相中发生中和时，可产生纳米气溶胶。 用分子离子和带电荷的纳米簇中和电纺丝纳米纤维可能导致纤维状气溶胶或游离纳米垫的形成。 纳米垫也可以在合适的基底上生产，形成有效的纳米过滤器。
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公开(公告)号：US20060275824A1
公开(公告)日：2006-12-07
申请号：US11397908
申请日：2006-04-05
IPC分类号： G06F19/00
CPC分类号： C30B7/00 , C30B29/58 , Y10T436/105831 , Y10T436/12 , Y10T436/25 , Y10T436/2575
摘要： The invention contemplates a method for recognition of proteins and other biological molecules by imaging morphology, size and distribution of crystalline and amorphous dry residues in droplets (further referred to as “crystallization pattern”) containing predetermined amount of certain crystal-forming organic compounds (reporters) to which protein to be analyzed is added. It has been shown that changes in the crystallization patterns of a number of amino-acids can be used as a “signature” of a protein added. It was also found that both the character of changer in the crystallization patter and the fact of such changes can be used as recognition elements in analysis of protein molecules.
摘要翻译： 本发明考虑了通过将含有预定量的某些成晶有机化合物的液滴（进一步称为“结晶图案”）的晶体和无定形干燥残余物的形态，尺寸和分布进行成像，鉴定蛋白质和其他生物分子的方法。 ）加入待分析的蛋白质。 已经表明，许多氨基酸的结晶模式的变化可以用作加入的蛋白质的“特征”。 还发现，结晶模式中变换器的特征和这种变化的事实都可以用作分析蛋白质分子的识别元件。
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公开(公告)号：US07918980B2
公开(公告)日：2011-04-05
申请号：US11397906
申请日：2006-04-05
申请人： Victor Morozov
发明人： Victor Morozov
IPC分类号： G01N27/453
CPC分类号： G01N33/5438 , G01N27/44769 , G01N2030/0035
摘要： A flow cell for electrophoretically-assisted capturing analytes from a flow. The flow cell includes a specimen chamber, a first membrane, a second membrane, a first electrode chamber, and a second electrode chamber. The specimen chamber may have a sample inlet and a sample outlet. A first portion of the first membrane may be coupled to a first portion of the specimen chamber. A first portion of the second membrane may be coupled to a second portion of the specimen chamber. The first electrode chamber may be configured to accept a charge. A portion of the first electrode chamber may be coupled to a second portion of the first membrane. A second electrode chamber may be configured to accept an opposite charge. A portion of the second electrode chamber may be coupled to a second portion of the second membrane.
摘要翻译： 用于电泳辅助从流中捕获分析物的流动池。 流动池包括试样室，第一膜，第二膜，第一电极室和第二电极室。 样品室可以具有样品入口和样品出口。 第一膜的第一部分可以联接到样品室的第一部分。 第二膜的第一部分可以联接到样品室的第二部分。 第一电极室可以被配置为接受电荷。 第一电极室的一部分可以耦合到第一膜的第二部分。 第二电极室可以被配置为接受相反的电荷。 第二电极室的一部分可以联接到第二膜的第二部分。
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5.发明授权公开(公告)号：US07189365B1
公开(公告)日：2007-03-13
申请号：US09674304
申请日：2000-03-03
申请人： Yutaka Yamagata , Tsunehiko Ozaki , Victor Morozov , Kozo Inoue
发明人： Yutaka Yamagata , Tsunehiko Ozaki , Victor Morozov , Kozo Inoue
IPC分类号： B01L3/02
CPC分类号： G05D9/12 , Y10T137/7287 , Y10T137/7303 , Y10T137/731 , Y10T137/7313
摘要： In a liquid treating equipment to maintain a small amount of liquid to a desired liquid level with constantly supplying an additional liquid into a small capacity vessel, a storage vessel 13 to store the small amount of liquid, an injection tube 16 to inject the small amount of liquid into the storage vessel 16, liquid supplying apparatus 23–25 to supply the small amount of liquid into the storage vessel through the injection tube, a flow path 15 of which one end is joined with the storage vessel, a discharge vessel 14, joined with the storage vessel 13 via the flow path, having a larger surface area than that of the storage vessel, a discharge tube 17 of which the discharging inlet is positioned at the same level position as a desired liquid level of the small amount of liquid in the storage vessel and liquid discharging apparatus 27–29 to discharge the liquid from the discharge vessel through the discharge tube are provided. Even though the liquid level in the discharge vessel is largely fluctuated, the liquid level in the storage vessel is almost never done.
摘要翻译： 在液体处理设备中，将少量液体维持在期望的液面上，同时向小容量容器中供应额外的液体，存储容器13以储存少量液体，注射管16注入少量 的液体进入储存容器16，液体供应装置23-25通过注射管将少量液体供应到储存容器中，其一端与储存容器接合的流路15，放电容器14， 经由流路与储存容器13连接，具有比储存容器更大的表面积;排出管17，其排出口位于与少量液体的期望液面相同的水平位置 在存储容器和液体排出装置27-29中，通过放电管将液体从放电容器排出。 即使放电容器中的液位大大波动，储存容器中的液面几乎从未完成。
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公开(公告)号：US07871824B2
公开(公告)日：2011-01-18
申请号：US11397911
申请日：2006-04-05
申请人： Victor Morozov
发明人： Victor Morozov
IPC分类号： G01N35/08
CPC分类号： B01L3/502715 , B01L2200/027 , B01L2200/0689 , B01L2300/0636 , B01L2300/0822 , B01L2300/0877 , G01N21/05 , G01N2021/0346 , Y10T436/117497
摘要： A flow chamber having a vacuum chamber and a specimen chamber. The specimen chamber may have an opening through which a fluid may be introduced and an opening through which the fluid may exit. The vacuum chamber may have an opening through which contents of the vacuum chamber may be evacuated. A portion of the flow chamber may be flexible, and a vacuum may be used to hold the components of the flow chamber together.
摘要翻译： 具有真空室和试样室的流动室。 样品室可以具有可以引入流体的开口和流体可以通过该开口离开的开口。 真空室可以具有可以抽空真空室的内容物的开口。 流动室的一部分可以是柔性的，并且可以使用真空来将流动室的部件保持在一起。
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7.发明授权公开(公告)号：US07368288B2
公开(公告)日：2008-05-06
申请号：US11397908
申请日：2006-04-05
IPC分类号： G01N21/00
CPC分类号： C30B7/00 , C30B29/58 , Y10T436/105831 , Y10T436/12 , Y10T436/25 , Y10T436/2575
摘要： The invention contemplates a method for recognition of proteins and other biological molecules by imaging morphology, size and distribution of crystalline and amorphous dry residues in droplets (further referred to as “crystallization pattern”) containing predetermined amount of certain crystal-forming organic compounds (reporters) to which protein to be analyzed is added. It has been shown that changes in the crystallization patterns of a number of amino-acids can be used as a “signature” of a protein added. It was also found that both the character of changer in the crystallization patter and the fact of such changes can be used as recognition elements in analysis of protein molecules.
摘要翻译： 本发明考虑了通过将含有预定量的某些成晶有机化合物的液滴（进一步称为“结晶图案”）的晶体和无定形干燥残余物的形态，尺寸和分布进行成像，鉴定蛋白质和其他生物分子的方法。 ）加入待分析的蛋白质。 已经表明，许多氨基酸的结晶模式的变化可以用作加入的蛋白质的“特征”。 还发现，结晶模式中变换器的特征和这种变化的事实都可以用作分析蛋白质分子的识别元件。
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公开(公告)号：US6033913A
公开(公告)日：2000-03-07
申请号：US879513
申请日：1997-06-20
申请人： Victor Morozov , Tamara Morozova
发明人： Victor Morozov , Tamara Morozova
IPC分类号： G01N33/483 , C12M1/00 , C12N15/09 , C12Q1/68 , G01N3/04 , G01N11/16 , G01N33/53 , G01N33/543 , G01N33/566 , G01N33/68 , G01N33/48
CPC分类号： G01N33/54353 , G01N3/04 , G01N33/54366 , G01N11/16 , Y10S977/704
摘要： A method and apparatus for measuring ligand binding to DNA or protein uses changes in the mechanical properties of strips of the DNA or protein to detect the formation of ligand complexes. Samples strips are prepared by pouring protein solution onto a glass surface, drying the solution, and causing the protein to be cross-linked; the cross-linked film is scraped off, cut into strips, and mounted in the stress/strain measuring apparatus, which includes force and strain transducers. The sample is held by arms and can be immersed into solutions. The arms are attached to transducers. The apparatus includes a sample holder. The sample films may include end reinforcements and may be pinned or glued to the arms.
摘要翻译： 用于测量配体与DNA或蛋白质结合的方法和装置使用DNA或蛋白质条的机械性质的变化来检测配体复合物的形成。 通过将蛋白质溶液倒入玻璃表面，干燥溶液并使蛋白质交联而制备样品条; 将交联膜刮去，切成条，并安装在包括力和应变传感器的应力/应变测量装置中。 样品由手臂夹持，可以浸入溶液中。 臂连接到换能器上。 该装置包括样品架。 样品膜可以包括端部增强件，并且可以被固定或胶合到臂上。
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公开(公告)号：US20120076694A1
公开(公告)日：2012-03-29
申请号：US12891135
申请日：2010-09-27
IPC分类号： G01N30/00
CPC分类号： G01N33/543 , G01N27/447
摘要： Analytes using an active assay may be detected by introducing an analyte solution containing a plurality of analytes to a lacquered membrane. The lacquered membrane may be a membrane having at least one surface treated with a layer of polymers. The lacquered membrane may be semi-permeable to nonanalytes. The layer of polymers may include cross-linked polymers. A plurality of probe molecules may be arrayed and immobilized on the lacquered membrane. An external force may be applied to the analyte solution to move the analytes towards the lacquered membrane. Movement may cause some or all of the analytes to bind to the lacquered membrane. In cases where probe molecules are presented, some or all of the analytes may bind to probe molecules. The direction of the external force may be reversed to remove unbound or weakly bound analytes. Bound analytes may be detected using known detection types.
摘要翻译： 使用活性测定法的分析物可以通过将含有多种分析物的分析物溶液引入涂漆膜来检测。 漆膜可以是具有用聚合物层处理的至少一个表面的膜。 漆膜可能对非分析物半渗透。 聚合物层可以包括交联聚合物。 可以将多个探针分子排列并固定在涂漆膜上。 可以将外力施加到分析物溶液以将分析物移动到漆膜。 运动可能导致部分或全部分析物结合到漆膜上。 在存在探针分子的情况下，部分或全部分析物可能与探针分子结合。 外力的方向可以颠倒以去除未结合或弱结合的分析物。 可以使用已知的检测类型检测结合的分析物。
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10.发明申请Apparatuses for Detecting and Recognizing Analytes Based on Their Crystallization Patterns 审中-公开基于其结晶模式检测和识别分析物的装置公开(公告)号：US20110105344A1
公开(公告)日：2011-05-05
申请号：US12941650
申请日：2011-01-19
IPC分类号： C40B30/00
CPC分类号： C30B7/00 , C30B29/58 , Y10T436/105831 , Y10T436/12 , Y10T436/25 , Y10T436/2575
摘要： The invention contemplates apparatuses for recognition of proteins and other biological molecules by imaging morphology, size and distribution of crystalline and amorphous dry residues in droplets (further referred to as “crystallization patterns”) containing predetermined amount of certain crystal-forming organic compounds (reporters) to which protein to be analyzed is added. Changes in the crystallization patterns of a number of amino-acids can be used as a “signature” of a protein added. Also, changes in the crystallization patterns, as well as the character of such changes, can be used as recognition elements in analysis of protein molecules.
摘要翻译： 本发明考虑通过成像含有预定量的某些结晶形成有机化合物（记者）的液滴中的结晶和无定形干燥残余物（进一步称为“结晶图案”）的形态，尺寸和分布来识别蛋白质和其它生物分子的装置， 加入待分析的蛋白质。 许多氨基酸的结晶模式的变化可以用作加入的蛋白质的“特征”。 此外，结晶模式的变化以及这种变化的特征可以用作分析蛋白质分子的识别元件。
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